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Citations for Bio-Rad :
3651 - 3700 of 4440 citations for Human Brain Derived Neurotrophic Factor BDNF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µg of RNA from each sample was reverse-transcribed in 40 µl using iScript™ cDNA Synthesis Kit (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... proteins from SDS-PAGE gels were transferred onto nitrocellulose membranes using the Transblot Turbo RTA Midi Transfer kit (BioRad, USA). Mouse anti-α-tubulin antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine RNA concentrations and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 200 ng of RNA ...
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bioRxiv - Biochemistry 2021Quote: ... 500 ng of DNaseI-treated RNA was used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative RT-PCR was performed using SsoAdvanced SYBR green supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was then treated for 45 min at room temperature with the appropriated HRP-conjugated secondary antibody: Proteins were visualized using an ECL+ detection kit (Amersham) and imaged using a Chemidoc Touch Imaging system (Biorad). Densitometry was analyzed using the ImageLab software.
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bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
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bioRxiv - Cancer Biology 2022Quote: ... About 500 ng of total RNA was used for cDNA synthesis (iScript cDNA synthesis kit, Bio-Rad, Hercules, California, USA). cDNA was diluted 1:5 before use in PCR reactions ...
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bioRxiv - Molecular Biology 2022Quote: ... 500ng for males per sample) was subsequently reverse-transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.)
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bioRxiv - Molecular Biology 2019Quote: ... The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad, USA). Protein bands were visualized using Gel DocTM XR+ System (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse transcription was performed on 0.5-1μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:10 in nuclease-free water ...
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bioRxiv - Developmental Biology 2019Quote: ... the samples were run in a 12% SDS-polyacrylamide gel and Western blots were developed using an ECL Western blotting kit (Biorad). Rabbit anti-countin antibodies were used at 1 ...
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bioRxiv - Genomics 2019Quote: Two hundred clonal adult male worms were used to prepare high molecular weight genomic DNA using CHEF Genomic DNA Plug Kits (BioRad). The DNA was quantified on a FEMTO Pulse® ...
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bioRxiv - Developmental Biology 2019Quote: ... Homogenates were centrifuged (11,000 rpm, 10 min, 4°C) and resultant supernatant assayed using a DC Protein Assay Kit (Bio-Rad). A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad) ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). RUNX1 and ACTB were amplified in parallel in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Physiology 2021Quote: ... the concentration of protein in the whole homogenates was determined with the DC protein assay kit (Bio-Rad, Hercules, CA). Equal amounts of protein from each sample were dissolved in Laemmli buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Complementary DNA (cDNA) was made by converting at least 100 ng RNA with the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2021Quote: Small pieces of dissected frozen pancreatic tumor tissues were collected and lysed (Bio-Plex Cell Lysis Kit, BioRad, USA; 171304011), followed by a repeated procedure of vortexing ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized on 1μg of isolated RNA by reverse transcription with random hexamer primers using the iScript Select cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the gels were washed in ddH2O and then silver stained following the manufacturers instruction (Silver Stain Plus Kit, 1610449, Biorad).
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bioRxiv - Microbiology 2021Quote: After the protein concentration of cell lysates and homogenates had been determined using the DC Protein Assay Kit (Bio-Rad) 20 µg of each sample were dissolved in NuPAGE LDS Sample buffer (including 2% β-mercaptoethanol) ...
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bioRxiv - Neuroscience 2020Quote: ... Results were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories, California, USA).
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bioRxiv - Neuroscience 2020Quote: ... Data were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories, California, USA).
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bioRxiv - Cell Biology 2021Quote: ... Gene expression analysis was conducted with 50 ng RNA using iTaq Universal One-Step RT-qPCR Kit (Bio-rad, 1725140). The reaction was carried out according to the manufacturer’s instructions using CFX Connect Real-Time PCR Detection System (Bio-rad ...
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bioRxiv - Immunology 2021Quote: ... Total protein content was measured by the Pierce BCA Protein Assay Kit (Thermo) and equivalent protein levels were loaded into an polyacrylamide gel (BioRad). Samples were transferred onto a nitrocellulose membrane using a semi-dry transfer apparatus before blocking with a 5% skim milk solution for thirty minutes at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Equal amounts of RNA (1.5 µg per sample) were reverse transcribed using an iScript Advanced cDNA synthesis kit (Bio-Rad). Resulting cDNA was diluted 1:50 in ultrapure water ...
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bioRxiv - Developmental Biology 2019Quote: ... Clarified protein lysates were measured according to standard methods using a DC protein assay kit (Bio-rad, Mississauga, Ontario, Canada). Bradford assays (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... RNA quality was assessed with an Experion Automated Electrophoresis Station using the Experion RNA StdSens analysis kit (BioRad, Hercules, CA) and yield was quantified with an ND-1000 spectrophotometer (Nanodrop ...
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bioRxiv - Neuroscience 2019Quote: ... conjugated secondary antibody (1:10 000) was subsequently applied and visualized by reaction with chemiluminesence reagent (Clarity Western Blotting Substrates Kit, BioRad) on radiography film (Carestream Blue X-ray film).
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bioRxiv - Genetics 2020Quote: ... Cell lysates were clarified by centrifugation at 6,000 rpm for 7 min and protein concentration was determined using a DC protein assay kit (Bio-Rad). Samples containing equal amounts of protein were brought to a total volume of 1 ml with appropriate buffer.
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bioRxiv - Physiology 2021Quote: RNA was isolated using a Macherey-Nagel NucleoSpin RNA kit and cDNA was synthesized using iScript RT Supermix (Bio-Rad). Samples were run on a Bio-Rad CFX96 Touch to assess mRNA levels of BNP ...
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bioRxiv - Biochemistry 2021Quote: The 100µl plasma was inactivated by heating at 56°C for 30 min 10 .60µl plasma was used for albumin and globulin depletion using an Aurum serum mini kit (BioRad, USA). The six depleted plasma samples were used for SWATH-MS analysis ...
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bioRxiv - Physiology 2020Quote: ... and circulating factor concentrations were measured using the following kits according to the respective manufacturer’s protocols: Bio-Plex protein array system (Biorad, UK) with one multiplex panel was used to measure total Glucagon-like peptide-1 (GLP-1) ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred to PVDF membranes using Trans-Blot Turbo RTA Mini 0.45 mM LF PVDF Transfer Kit (Bio-Rad), and blotted with anti-HA antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein transfer to the PVDF membrane was performed following manufacturer’s instructions for the membrane kit (Trans-Blot Turbo Midi 0.2 μm PVDF, 1704157, BioRad, Hercules, CA) and the transfer apparatus (Trans-Blot® Turbo™ Transfer System ...
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bioRxiv - Genetics 2021Quote: ... Immunolabelling was visualized using chemiluminescence kits (SuperSignal West Pico PLUS Chemiluminescent Substrate; ThermoScientific) on a Chemidoc MP Imaging System (Biorad) and analyzed with the ImageLab Software (version 5.2 ...
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bioRxiv - Neuroscience 2021Quote: ... A qScript cDNA Synthesis kit (QuantaBio, Beverly, MA, USA) and T100 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) were used to generate the cDNA ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA was used for RNA-seq analysis or prepared for qRT-PCR using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was used as template in SYBR green qPCR supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: qPCR was performed using the iTaq universal SYBRGreen® kit following the manufacturer protocol (Bio-Rad Laboratories Inc, Hercules, CA) on an Agilent Mx3005P qPCR Systems (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... was added to the samples according to the manufacturer’s instruction and equivalent amounts of protein (30 μg as measured using the DC Protein Assay Kit (Bio-Rad) and proteins were separated on a 4 to 12% on a 12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: Thymi isolated from Foxp3hCD2 x Rag1GFP reporter mice were homogenized and lysed using Bio-Plex Cell Lysis Kit (BioRad Laboratories), and the protein content of the thymic lysates was measured using Pierce™ BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... 100-200 ng of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad) on an Arktik thermal cycler (Thermo scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes went through a final TBST wash and then visualized on the ChemiDoc XRS+ using the Clarity Western ECL kit (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... elegans cDNA was amplified from extracted mRNA from wild type worms using the iScript Select cDNA Synthesis kit (Bio-Rad). KLP-18 cDNA was amplified from whole worm cDNA with gene specific primers and Q5 DNA polymerase ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA was reverse transcribed with oligo(dT) primers for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad). The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized using 1 µg of total RNA and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). Synthesized cDNAs were then quantified by real time quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: The secretome samples were fractionated by one-dimensional SDS-PAGE with the Mini-protean® 3 kit (Bio-Rad, USA) using a 12% resolving gel and a 5% stacking gel at 200 V for 45 min ...