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Citations for Bio-Rad :
3601 - 3650 of 4440 citations for Human Brain Derived Neurotrophic Factor BDNF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Detection of viral RNA using one-step real-time reversetranscription PCR was performed according to (Corman et al., 2020) with iTaq Universal Probes One-Step Kit (Biorad) using primers and probes against the N-gene (N_Sarbeco_F ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using the SsoAdvanced universal SYBR green supermix kit (Bio-Rad, Bio-Rad CFX connect RT-PCR machine). Custom primers were used for gene amplification (Table 2 ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using the SsoAdvanced universal SYBR green supermix kit (Bio-Rad, Bio-Rad CFX connect RT-PCR machine). Custom primers were used for gene amplification (Table 2 ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was synthesized from 1 μg of isolated RNA using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05 ...
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bioRxiv - Biochemistry 2021Quote: ... Total RNA from ddHEKs and ddMEFs were harvested utilizing the Aurum Total RNA Mini kit (Bio-Rad, Hercules, CA, USA) and DNase treated on-column ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6) were quantified using a real-time PCR detection system and SsoAdvanced Universal SYBR Green Supermix kit (BIO-RAD). In brief ...
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bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The purified RNA was quantified and an equal amount of RNA was used for cDNA preparation using iScript cDNA synthesis kit (Biorad). GAPDH was used as a housekeeping gene was used ...
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bioRxiv - Neuroscience 2020Quote: ... We used 1 microgram of the total RNA for cDNA synthesis (iScript cDNA Synthesis Kit, Cat. #170-8891, Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Mini-PROTEAN TDX Precast Protein Gels and Trans-Blot Turbo RTA Mini PVDF Transfer Kit were purchased from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Cell Biology 2021Quote: ... The concentrations of the extracts were determined using the Bio-Rad Protein assay kit (Bio-Rad Laboratories Inc. Hercules, CA). The extracts were then aliquoted into individual tubes ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was developed by using ECL prime western blotting detection kit (Amersham) and images were captured using Versadoc (Biorad).
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bioRxiv - Cell Biology 2021Quote: ... and the RNA was reverse transcribed into cDNA using an iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). Quantitative PCR was performed with RT2 SYBR Green qPCR Master Mix (QIAGEN ...
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bioRxiv - Plant Biology 2021Quote: ... Up to 500 ng of RNA was used for reverse transcription using either the iScriptTM cDNA synthesis kit (Bio-Rad) or the qScript cDNA Synthesis Kit (Quantabio) ...
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bioRxiv - Plant Biology 2020Quote: ... the soybean AATRhg1 and GmRBOHC2 (Glyma.06G162300.1) ORFs were PCR-amplified from Williams82 cDNA generated by the iScript cDNA Synthesis Kit(Bio-Rad) and KAPA HiFi polymerase (Kapa Biosystems) ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA was synthesized from 1 µg DNA-free RNA using the iSCRIPT cDNA synthesis kit (BioRad, Hercules, CA, U.S.A). Real-time PCR master mixes were prepared using iQ sybr Green Mix (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... The purified total RNAs were converted to first-strand cDNAs by using a reverse transcription kit (Bio-Rad, Cat#1708891). The relative levels of HIV-1 un-spliced transcript and human MMP-2 mRNA were measured by qRT-PCR using the primers 5’AGGGACCTGAAAGCGAAAG3’ (HIV-1 un-spliced-forward ...
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bioRxiv - Genetics 2021Quote: ... RNA quantity and quality were determined on a NanoDrop 2000c (PeqLab) and cDNA was prepared using the iScript cDNA Synthesis Kit (BioRad). To quantify RNA expression ...
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bioRxiv - Microbiology 2021Quote: ... purified RNA (1000 ng) was converted to cDNA by using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) following the manufacturer’s standard procedure ...
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bioRxiv - Microbiology 2021Quote: ... and relative transcript levels were measured for each sample in triplicate by quantitative qRT-PCR using SYBR Green PCR Kit (Biorad), on a MyiQ™ Real time PCR system (Biorad) ...
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bioRxiv - Microbiology 2020Quote: ... IL-10) responses were measured using a customized Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Assay kit (Biorad, USA) with the BioPlex 200 platform according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2021Quote: ... DNAse treatment were performed as in (Gigli-Bisceglia et al., 2018; Savatin et al., 2014) and cDNA synthetized with iScript cDNA Synthesis Kit (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: cDNA synthesis and quantitative reverse transcription PCR (qRT-PCR) were performed using the iTaq Universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Complementary DNA (cDNA) from extracted RNA was obtained using the iScript™ cDNA Synthesis Kit (Bio-rad, CAT No.:1708890) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... DNAase treatment and cDNA synthesis were done with the iSript gDNA clear cDNA synthesis kit (BIO-RAD, Hercules, CA, USA). Quantitative reverse transcription-polymerase chain reaction (RT-PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 µg of total RNA was reverse transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Feldkirchen, Germany). Real-time PCR was performed using Platinum SYBR Green qPCR Super Mix-UDG (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... qPCR was performed using the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad, Cat No.: 172-5150) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The amounts of isolated mitochondria were expressed as the protein concentration using a Bio-Rad protein assay kit (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed three times with TBST and were developed using a chemiluminescent ECL kit (Pierce, Cat # 32106) and images were acquired using a ChemiDocTM imaging system (BioRad).
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bioRxiv - Cancer Biology 2020Quote: The isolated total RNA (1 μg) was reverse-transcribed to produce cDNA using iScript Reverse Transcription Supermix kit (Bio-Rad). Real-time PCR was performed using SYBR Green supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... A standardized amount of RNA was transcribed into cDNA using the iScript Advanced cDNA Synthesis Kit (Bio-Rad; 170-8843), and cDNA was then amplified using the primers ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were treated with an enhanced chemiluminescence kit according to the user manual and developed using a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Cancer Biology 2022Quote: Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The protein content of the LDL sample was determined by a protein assay kit (Bio-Rad Laboratories, Inc., Tokyo, Japan) based on the Bradford method using bovine serum albumin as a standard ...
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bioRxiv - Developmental Biology 2022Quote: ... 1ug RNA was in a 20ul reaction volume to generate cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad, 1708891). Typically ...
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bioRxiv - Microbiology 2022Quote: ... we synthesized first-strand cDNA from cellular RNA isolated (100ng) from each sorted sample using iScriptTM cDNA synthesis kit (BioRad). Each rtPCR reaction included 25°C for 5 mins ...
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bioRxiv - Neuroscience 2022Quote: ... an equal amount (500 ng) of RNA was reverse transcribed to cDNA using iScript reverse-transcription kit (Bio-rad, 1708841). Quantitative real time PCR (45 cycles – LightCycler480 software ...
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bioRxiv - Microbiology 2022Quote: ... Viral copy numbers were assessed via digital-droplet PCR (ddPCR) using SARS-CoV-2 ddPCR kit (#12013743) from Bio-Rad with N1 and N2 primers and probes targeting the nucleocapsid genes ...
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bioRxiv - Microbiology 2022Quote: HCV RNA copies were determined by Taqman RT-qPCR using a Quanta BioSciences qScript XLT One-Step RT-qPCR kit according to the manufacture’s instruction (Bio-Rad). GLT1_probe was used to detect for GLT1 RNA ...
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bioRxiv - Cell Biology 2022Quote: ... following the manufactures instructions and cDNA was synthesized from 1μg of total RNA using iScript cDNA Synthesis Kit (Bio-Rad). QPCR was performed on a CFX96 real-time PCR thermocycler (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Up to 1 µg of total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) or gDNA Clear iScript cDNA Synthesis Kit for patient-derived tumor cells (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... The blots were developed with an enhanced chemiluminescence kit (Thermo Fischer Scientific) and signals acquired by a ChemiDoc instrument (BioRad). All primary histone variant-specific and H3 marks-specific antibodies were used at 1 µg/ml dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) in the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of whole protein from each sample was subjected to SDS-PAGE using the TGX Stain-Free FastCast Acrylamide kit (BioRad). Protein bands were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was quantified via a one-step qRT-PCR using the iTaq universal probes one-step kit (Bio-Rad) per the manufacturer’s instructions for a 20µL reaction ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 1 μg of purified RNA using the iScript cDNA synthesis kit (Cat. no. 1708891, Bio-Rad). Quantitative real-time PCR assays were performed using the iQ SYBR Green Supermix (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Blots were developed using the SuperSignal West Pico PLUS chemiluminescent substrate kit (Thermo) and imaged using the ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed and normalised for protein concentration using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Inc. # 5000001) and a spectrophotometer ...
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bioRxiv - Plant Biology 2021Quote: ... Quantification of RNA sample quality and concentration was performed using the Experion ‘StdSens’ kit (Bio-Rad Laboratories, Inc., Hercules, CA). Stranded RNA-Seq libraries were created and quantified by qPCR (2×76 bp or 2×151 bp ...