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Citations for Bio-Rad :
3551 - 3600 of 4440 citations for Human Brain Derived Neurotrophic Factor BDNF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 1µg total RNA per sample using a Bio-Rad cDNA Synthesis Kit (Bio-Rad Laboratory). Quantitative real-time polymerase chain reaction (RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: cDNA was synthesized from 500 ng RNA using the iScript™-cDNA Kit (Bio-Rad Laboratories, Hercules, CA, USA). Real-time quantitative PCR was carried out using mRNA-specific primers (Table S1) ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was performed in triplicate with a PCR starter kit in CFX96 Real-Time System (Bio-Rad, USA). hsa-miR-16-5p was used as an internal control to normalize the gene expression ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes ...
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bioRxiv - Genomics 2024Quote: ... Total protein concentration was determined by a detergent compatible DC™ Protein Assay Kit II (Bio-Rad Laboratories, #5000112). For Western blot analysis ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μg of total RNA was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Standard curves were run for all primer pairs to ensure high efficiencies (>97% ...
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bioRxiv - Plant Biology 2024Quote: The AtGCN5 coding sequence amplified from Arabidopsis Col-0 cDNA using iProof™ High-Fidelity PCR Kit (Bio-Rad) was inserted into pDONR221 vector (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were transferred to Nitrocellulose membrane with the Trans-Blot Turbo RTA transfer kit (BIO-RAD Cat.#170-4270). The chemiluminescent signal was detected with Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... was used to determine RNA concentrations and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 200 ng of RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 µg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). FCGRT ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted using the Total RNA Kit I (EZNA) and cDNA synthesis was carried out using iScript (Biorad) according to the manufacturers instructions and using 300 ng total RNA for each sample.
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bioRxiv - Cell Biology 2020Quote: ... RNA (1µg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... We used SuperSignal West Pico or Dura chemiluminescent detection kits (Thermo Fischer) and the chemiluminescent signal was acquired using the ChemiDoc system equipped with an XRS camera (BioRad).
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bioRxiv - Cell Biology 2020Quote: ... 500ng of RNA was reversed transcribed into cDNA in a 20 μL reaction volume using iScript cDNA synthesis kit (Biorad) in accordance to manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... Quantity of libraries was assessed by performing digital droplet PCR (ddPCR) with Library Quantification Kit for Illumina TruSeq (BioRad, #1863040). Libraries were diluted to 2 nM and paired-end sequencing was performed on a HiSeq 2500 sequencer (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized using 600 ng input total RNA per 40 ¼l reaction with the iScript cDNA Synthesis Kit (BioRad), cycling for 5 min at 25°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was washed three times with TBST and detection was performed with the Clarity™ ECL kit (Bio-Rad) according to the manufacturer’s instructions and visualized with a CCD camera of the Odyssey Fc imaging system (Li-COR Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... was used to isolate the total RNA followed by cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad, 1708891). Real-time qPCR quantification was done on a Bio-Rad CFX96 Real-Time system using SsoFast EvaGreen Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA was harvested as described above and used to create cDNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories). qPCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... Synthesis of cDNA was performed in duplicate from 500ng RNA in 10μL reactions using the iScript Advanced cDNA Synthesis kit (Bio-Rad). Reactions were diluted 1:5 in water ...
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bioRxiv - Neuroscience 2020Quote: ... 500 ng of RNA was used for Realtime RT-PCR analysis using iTaq universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The RNA was then cleared of genomic DNA using DNAse from an iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 65 −175 ng of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Target-specific primers designed with the assistance of the free web-based PrimerQuest® Tool (IDT ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with random primers using an iScript cDNA synthesis kit (Bio-Rad) and analyzed with a Bio-Rad CFX96 real time system ...
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bioRxiv - Immunology 2022Quote: ... 1-0.2 μg of RNA was used to synthesize cDNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA template was mixed with iTAQ SYBR green universal super mix (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA (cDNA) was synthesized from 500 ng of purified RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad) following protocols provided by the manufacturer.
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared and analyzed using a Bio-Plex Pro Mouse Cytokine 23-Plex Assay kit (Bio-Rad, Hercules, CA). Assays were performed according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... RT-PCR reactions were carried out using the Bio-Rad One-Step RT-ddPCR Advanced Kit for Probes (BioRad, 1864021) following the manufacturer’s protocol with the following reaction conditions ...
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bioRxiv - Biochemistry 2019Quote: ... Protein content was measured using the BCA kit and 50 micrograms of protein per sample were loaded on a Bio Dot apparatus (Biorad) equipped with a 0.2 micron pore size nitrocellulose membrane soaked in buffer B (10 mM Tris-HCL pH 8.0 ...
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bioRxiv - Genomics 2019Quote: ... The exact molarity of the pools were assess by qPCR using the KAPA Library Quantification Kit Illumina on CFX96 system (Biorad). Then each pool was sequenced on 1 flowcell of HiSeq 2000 system (paired-end ...
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bioRxiv - Cancer Biology 2019Quote: ... according to the manufacture’s protocol and 1 µg RNA was used to convert to cDNA using iScript Select cDNA Synthesis Kit (Bio-Rad) in the presence of both oligo (dT ...
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bioRxiv - Molecular Biology 2019Quote: ... Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce) and imaged and quantified with a ChemiDoc MP Imaging System (BioRad). For quantification of Cas9 protein in dCas9-KRAB and dCas9-VPR parental clones ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse transcription of 1 μg of total RNA per sample was performed using the iScript cDNA synthesis kit (Bio-RAD). Transcript levels were determined by quantitative real-time RT-PCR as previously reported [14] using primers provided in Supplementary Table 1 and normalizing expression to ATCB transcript levels.
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bioRxiv - Microbiology 2019Quote: ... The antigen-antibody reaction was analyzed by using Opti-4CN detection kit according to the instructions of the manufacturer (Biorad).
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bioRxiv - Physiology 2020Quote: ... The integrity of the extracted RNA samples was additionally determined by using an Experion RNA StdSens Analysis Kit (Bio-Rad). Any samples showing poor RNA quality were also excluded from further analysis ...
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bioRxiv - Neuroscience 2020Quote: cDNA was synthesized from total RNA isolated from LCLs by using a reverse transcription kit iScriptTM cDNA synthesis (1708891, Biorad). The reaction was performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The quality of the resulting RNA library was assessed using the Experion RNA Analysis Kit (Bio-Rad; Hercules, CA, USA), and only the samples with an integrity number 8-10 were selected for transcriptomic analysis ...
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bioRxiv - Physiology 2020Quote: ... Protein concentration was determined in triplicate with a commercial colorimetric assay (Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia), against bovine serum albumin standards (BSA ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System RTA kit following the manufacturer’s protocols (Bio-Rad). Subsequently ...
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bioRxiv - Molecular Biology 2019Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified using Qbit dsDNA HS Assay kit (Invitrogen) ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension is then embedded in 1% low-melt agarose plugs and treated using the CHEF Mammalian Genomic DNA Plug Kit (Biorad), according to the manufacturer’s protocol38,39 ...
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bioRxiv - Microbiology 2019Quote: ... 1 µg total RNA was subjected to random-primed cDNA synthesis using an iScript cDNA Synthesis kit following the manufacturer’s protocol (Bio-Rad). 1 µL of the resulting cDNA was used in triplicate 25-µL qRT-PCR reactions ...
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bioRxiv - Plant Biology 2019Quote: ... The amount of protein recovered after lysis was determined using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad), with bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized from pre-TRAP and TRAP RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad, #170-8841). mRNA expression was measured using TaqMan Fast Advanced Master Mix (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1µg of RNA from each sample was converted to double stranded cDNA using iScript cDNA synthesis kit (Bio-Rad). RT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were removed at stated time points and secreted cytokine quantities were measured using Luminex Bead technology according to Bioplex protocols and cytokine Kit (Biorad).
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 0.5 µg of total RNA using a RevertAid Premium Kit (Fermentas) and subjected to RT-qPCR using iTaq Universal SYBR Green Supermix (BioRad) and 10 ng of cDNA per reaction ...
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bioRxiv - Neuroscience 2020Quote: ... RNA (1 μg) was reverse-transcribed to cDNA with the iScript ™ cDNA Synthesis Kit (Bio-Rad Laboratories, CA, USA) according to manufacturer’s protocol ...