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Citations for Bio-Rad :
3901 - 3950 of 4440 citations for Human Brain Derived Neurotrophic Factor BDNF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and 30 ng of RNA from each sample was reverse transcribed using the iScript™ cDNA Synthesis Kit (1708891; Bio-Rad). Real-time PCR was performed using Applied Biosystems™ TaqMan™ Gene Expression Master Mix (4369016 ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA was quantified on a Bio-Rad T100 96-Well PCR Gradient Thermal Cycler with the SYBR Green kit (Bio-Rad, SsoAdvanced™ Universal SYBR® Green Supermix) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Lysates from the ribopuromycylation assays were loaded into gels prepared using the TGX Stain-Free FastCast Acrylamide Kit (Bio-Rad, 1610183) (Fig ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then reverse transcription was performed by HiScript III RT SuperMix for qPCR kit (R323, Vazyme, Nanjing, China) by BIO-RAD iCycler(BIO-RAD ...
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bioRxiv - Systems Biology 2022Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2022Quote: One ng of total RNA was used for single-tube reverse transcription (RT) and ddPCR using the One-Step RT-ddPCR Advanced kit (Bio-Rad). Droplets were generated and subsequently analyzed using the QX100 system (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of total RNAs was used to generate cDNA using the iScript cDNA synthesis kit (Bio-Rad, Richmond, CA, USA). Real-time PCR was subsequently performed using the iTaq Universal SYBER Green Supermix using the primers listed in Supplemental Table 1 on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche) ...
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bioRxiv - Immunology 2022Quote: ... was converted to cDNA with the C1000 Touch Thermal Cycler using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad, 1708890), with the following modifications ...
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bioRxiv - Microbiology 2022Quote: ... about 25 ng of DNA-free total RNA for each lncRNA was reverse-transcribed using iTaq cDNA Synthesis Kit (Bio-Rad) and then analyzed with ABI 7500 Fast Real-time PCR System (Applied Biosystem ...
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bioRxiv - Plant Biology 2019Quote: ... RNA samples confirmed as free of genomic DNA were diluted to equivalent concentrations and subjected to cDNA synthesis with iScript cDNA Synthesis Kit according to the manufacturer’s instructions (BioRad, Hercules, CA). All cDNA synthesis reactions for a single time-point were performed simultaneously to decrease any inherent variability in the synthesis process ...
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bioRxiv - Genomics 2020Quote: ... Concentration and 260/280 ratios were quantified using a NanoDrop 1000 spectrophotometer before cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad). Primers were designed to span exon-exon boundaries wherever possible (Supplementary Table 2) ...
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bioRxiv - Genetics 2019Quote: ... Reverse transcription of 400ng of RNA into cDNA was completed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA) and the Veriti 96-well thermal cycler according to the manufacturer’s instructions (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... total RNA from Astyanax embryos of various stages (2-24 hpf) was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad) and amplified using the following primers:
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bioRxiv - Cancer Biology 2019Quote: ... membranes were incubated with secondary antibodies for one hour at room temperature and developed using Clarity ECL Detection Kit (Bio-Rad).
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bioRxiv - Genomics 2019Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2019Quote: ... Cells were collected and resuspended in OMNI Tagmentation Mix containing ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme that are parts of the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad). The OMNI Tagmentation Mix was buffered with 1X PBS supplemented with 0.1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... was performed using 300 ng RNA with the CFx Connect Real-Time system with the iTaq Universal SYBR Green One Step Kit (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and was reverse transcribed to cDNA using either qScript XLT cDNA SuperMix (Quantabio) or iScript™cDNA Synthesis Kit (Bio-Rad). Samples were run on an Applied Biosystems Viia7 Real-time PCR system ...
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bioRxiv - Physiology 2019Quote: ... 1 μg of RNA was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170-8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of total RNA from all samples were transcribed into cDNA using iScript cDNA Synthesis Kits (Bio-Rad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD, USA) for sequencing analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... 500ng of RNA from clinical samples were converted to cDNA using the iScript cDNA synthesis kit from Biorad (Catalog number: 1708891). Primers that overlapped intron-exon junction were designed using the NCI Primer-blast software and are listed in Supplementary Table 13 ...
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bioRxiv - Pathology 2020Quote: ... 1 μg of total RNA was used as a template for reverse transcription assay using the iScript cDNA Synthesis Kit (Bio-Rad); cDNA was used for detecting FAM210A and GAPDH expression ...
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bioRxiv - Cell Biology 2021Quote: ... The peroxidase-conjugated secondary antibody was incubated and reacted using ECL kits (Cytiva).The signal was detected using ChemiDoc XRS + (Bio-Rad). The membrane was stripped using stripping buffer (strong ...
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bioRxiv - Microbiology 2019Quote: ... First-strand cDNA for gene expression analysis using real-time qPCR was carried out using the iScript cDNA synthesis kit from Bio-Rad following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 250 ng of RNA was used as a template in each reaction with iTaq Universal One-Step RT-qPCR Kit (Bio-Rad) or Luna Universal One-Step RT-qPCR Kit (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2020Quote: RNA extraction of positive samples of the GFP fluorescence assay as well as butanediol fermentation processes was performed using the Aurum Total RNA Mini Kit (BioRad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Reverse transcription: 200 ng of RNA was reverse transcribed using iScript Advanced cDNA Synthesis kit for RT-qPCR (1702537, Bio-Rad) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were resolved on SDS polyacrylamide gels and blotted onto PVDF membranes using Trans-Blot Turbo RTA Midi 0.45 μm LF PVDF Transfer Kit (Bio-Rad, 1704275). Transfer was run in Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was then developed by incubation with chemiluminescence (Amersham ECL prime kit) and imaged with a ChemiDoc™ XRS+ System (BIORAD). The intensity of GFP bands were quantified with Image J software ...
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bioRxiv - Bioengineering 2021Quote: ... Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves: an iScript cDNA synthesis kit (Bio-Rad) was used to reverse transcribe total RNA into cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Austria).52 The luciferase activity data was normalised to protein content measured by DC Protein determination kit (Bio-Rad Laboratories, Inc., USA) and SCO effect was expressed as a fold-change over untreated cells ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Immunology 2020Quote: ... the signals were detected using an enhanced chemiluminescence detection kit (Super Signal West Pico Plus, 34580, ThermoFischer Scientific) and membranes imaged with ChemiDoc Imaging System (Bio-Rad). The same anti-S1 and anti-S2 antibodies as well secondary antibodies were also used to detect the Spike protein on EVs by ELISA (see protocol below).
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bioRxiv - Molecular Biology 2020Quote: 500ng of genomic RNA were used for reverse transcription into cDNA using the iScript cDNA synthesis kit (Bio-Rad, #170-8891). cDNA was diluted 10X and 2uL was added for each qPCR reaction (iQ™ SYBR Green Supermix ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence substrate was then applied to the blot according to the manufacturer’s instructions (Bio-Rad Clarity™ Western ECL Substrate kit). The chemiluminescent signals were recorded using a Syngene G:BOX (Synoptics ...
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bioRxiv - Cancer Biology 2019Quote: Plasma activities of ALT and AST were assessed with the ALT and AST Reagent Kit (Biosystems Reagents) using a Benchmark Plus microplate spectrophotometer (Bio-Rad). Plasma concentration of non-sulfated bile acids were measured with the Bile Acid Assay Kit (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme (ATAC Tagmentation Buffer and Enzyme are both included in the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad)) ...
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bioRxiv - Genomics 2021Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2021Quote: ... One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD, 1708840). qPCR reactions for targeted genes were performed with the Luminaris HiGreen qPCR Master Mix (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
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bioRxiv - Microbiology 2021Quote: ... total 600 ng of RNA was used for cDNA synthesis by using Random hexamer oligonucleotide primer (iScript Select cDNA Synthesis Kit, BioRad, USA). Gene specific primers (Table S2 ...
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNAs were synthesized from 500 ng DNase-treated total RNA by RT with the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 µL according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane by semi-dry transfer using the Trans-Blot turbo RTA Mini PVDF transfer kit (Bio-Rad) and the Trans-Blot turbo system ...