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Citations for Bio-Rad :
3601 - 3650 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed using 350 ng (CD11B+) and 1,000 ng (brain) mRNA with an iScript cDNA synthesis kit (BioRad). Real-time quantitative PCR was performed on triplicate samples using SYBR Green Supermix (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Freshly isolated mRNA was reverse transcribed into complementary DNA with random primers by iScript cDNA Synthesis Kit (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Signal was detected using the HCL kit (ECLTM Primer Western Blotting Detection Reagent, AmershamTM) and a Chemidoc system (Biorad). Signal were quantified with the ImageLab Software.
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bioRxiv - Developmental Biology 2023Quote: ... total RNA samples were reverse transcripted into first-strand cDNA using the iScript Select cDNA Synthesis kit (Bio-rad). 1 µl of a 10X diluted cDNA sample was then used in the qPCR reaction with SsoAdvanced Universal SYBR Green Supermix (Bio-rad) ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using the iScript™ cDNA Synthesis Kit and iTaq Universal SYBR Green Supermix (Bio-Rad) and run on a Biorad CFX384 real-time system (C1000 Touch Thermal Cycler) ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was synthesized from 1 µg total input RNA using iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad 1725035). In a qPCR plate (Bio-Rad MLL9601) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared from 500ng RNA per reaction using the iScript cDNA Synthesis Kit (#1708891, Bio-Rad, Hercules, CA). QPCR was performed using probe-based primer sets and master mix (Prime Time ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of RNA were converted into cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA; 1708891) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of purified RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR analysis was performed using the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed to cDNA using the iScript™ cDNA Synthesis kit (Bio-Rad #1708890, Hercules, CA, USA), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... cDNA was generated from total RNA via reverse transcription reaction using an iScript RNA-to-cDNA kit according to the manufacturer’s instructions (BioRad). cDNA was then amplified using the iQ SYBR Green Supermix (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... RNA templates were then utilized for complimentary DNA (cDNA) synthesis utilizing the iScript cDNA synthesis kit protocol (Bio-Rad).
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bioRxiv - Genomics 2022Quote: ... 0.5 μg of purified RNA was reverse transcribed using either the iScript gDNA Clear cDNA Synthesis Kit (BioRad, #1725034) or Maxima H Minus First Stand cDNA Synthesis Kit (Thermo Fisher Scientific M1681) ...
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bioRxiv - Physiology 2022Quote: ... and the first-strand cDNA was prepared using the iScriptTM cDNA synthesis kit (Bio-Rad, #1708890, Hercules, CA, USA). qPCR analysis was performed with SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants were used for protein quantification via the use of the DC(tm) Protein Assay Kit (Bio-Rad). Equal loading of proteins (10–20 µg ...
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bioRxiv - Plant Biology 2022Quote: One microgram of RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative real-time PCR analysis was performed on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... One-step RT-qPCR was performed using an iTaq Universal SYBR Green One-Step RT-qPCR Kit (BIO-RAD). The reaction was performed in a total volume of 20 μL by mixing 10 μL iTaq universal SYBR Green reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Purified RNA was analyzed by TaqMan qRT-PCR one-step RT-PCR kit for probes (Bio-Rad laboratories, Italy) with a TaqMan RT-PCR instrument (CFX384 real time system ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 μ of total RNA using iScript gDNA clear cDNA synthesis kit (Bio-Rad, 1725035) with random primers ...
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bioRxiv - Microbiology 2023Quote: ... was used to purify RNA followed by cDNA synthesis (0.5 μg of RNA) using iScript RT kit (Bio-Rad) and qPCR was performed using the Brilliant II SYBR® Green QPCR master mix (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were blotted onto a nitrocellulose membrane using the Trans-Blot Turbo TRA Transfer Kit (Bio-Rad, Feldkirchen, Germany). Following protein transfer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunoblot was performed by transferring protein to a PVDF membrane using Trans-Blot Turbo RTA Transfer Kit (Biorad, 1704272) and the Biorad Transblot Turbo System ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was then incubated in a transfer buffer from the trans-blot turbo RTA transfer kit (Biorad, 1704272) for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... at 100 V and then transferred using the Trans-Blot Turbo transfer kit onto nitrocellulose membranes (Bio-Rad, #1704270). Membranes were blocked with 5% nonfat dry milk prepared in TBST (Tris-buffered saline with Tween 20 ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was extracted and purified from RPE tissue punches using Single Shot Cell Lysis Kit (Bio-Rad Laboratories). cDNA synthesis was performed from 1.5 μg RNA using RT² First Strand Kits (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: qRT-PCR was performed on a C1000 Thermal Cycler using SsoFast™ EvaGreen Supermix kit (Bio-Rad, Hercules, CA) according to the instructions ...
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bioRxiv - Immunology 2023Quote: ... The protein bands were visualized using an ECL kit (Meilunbio, CHN) and imaged on ChemiDoc XRS+ (Bio-Rad, USA).
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bioRxiv - Cancer Biology 2023Quote: Retrotranscription of RNA was performed using 3μg of total RNA in a 40μl final volume reaction mixture using iScript Advanced cDNA Synthesis Kit for RT-qPCR (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 500ng RNA was used as input for cDNA synthesis through iScript™ advanced cDNA synthesis kit (Bio-Rad 1725038) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was revealed with the Clarity™ Western ECL Substrates Kit (Bio-Rad Laboratories Inc, Hercules, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized using the iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories, Hercules, CA, USA) with 2.0-20 ng input corneal RNA according to a manufacturer protocol ...
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bioRxiv - Genomics 2024Quote: ... Total protein concentration was determined by a detergent compatible DC™ Protein Assay Kit II (Bio-Rad Laboratories, #5000112). For Western blot analysis ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μg of total RNA was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Standard curves were run for all primer pairs to ensure high efficiencies (>97% ...
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bioRxiv - Plant Biology 2024Quote: The AtGCN5 coding sequence amplified from Arabidopsis Col-0 cDNA using iProof™ High-Fidelity PCR Kit (Bio-Rad) was inserted into pDONR221 vector (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... 7µl nuclease-free water and 10 µl of SSo advanced universal SYBR Green qPCR kit (Bio-Rad, Ref: 1725272) for gill derived samples ...
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bioRxiv - Bioengineering 2024Quote: ... Sample concentrations were normalized to 1 ng/µL during cDNA preparation using the iScript Synthesis Kit (Bio-Rad, 1708841), following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 1µg total RNA per sample using a Bio-Rad cDNA Synthesis Kit (Bio-Rad Laboratory). Quantitative real-time polymerase chain reaction (RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: cDNA was synthesized from 500 ng RNA using the iScript™-cDNA Kit (Bio-Rad Laboratories, Hercules, CA, USA). Real-time quantitative PCR was carried out using mRNA-specific primers (Table S1) ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression was quantified using the iTaq Universal One-Step RT-qPCR Kit (cat. 1725150, BioRad, Hercules, CA, USA) in Applied Biosystems and normalized to GAPDH or Actb ...
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bioRxiv - Bioengineering 2024Quote: ... transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) at 25V ...
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bioRxiv - Molecular Biology 2024Quote: ... Five hundred nanograms of total RNA were used for a SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad). rRNA was depleted with Mouse/Rat riboPOOL (siTOOLs Biotech) ...
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bioRxiv - Molecular Biology 2024Quote: ... Four hundred nanograms of total RNA were used for a SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supernatant was recover and protein concentration was measured using the DC™ Protein Assay Kit II (Bio-Rad, 5000112). Lysates were boiled in the NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Neuroscience 2024Quote: ... Counts per minute (CPM) were quantified and normalised to overall protein using a DC protein assay kit II (Biorad).
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bioRxiv - Molecular Biology 2024Quote: ... Chemiluminescence was induced by the Chemi-Lumi One reagent kit (Nacalai Tesque) and detected with ChemiDoc Touch (Bio-Rad). The bands were quantified using ImageJ48 or Image Lab software (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of total RNA was used to prepare cDNA using iScript cDNA Synthesis Kit (Bio-Rad Laboratories #1708891) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were then determined via the RC DC Protein assay kit (Bio-Rad, Hercules, CA, USA, cat #: 5000121) and absorbance readings at 700 nm using a microplate spectrophotometer (Biotek Synergy H1 hybrid reader ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 300ng of total RNA was used to synthesize cDNA using the iScript kit reverse transcriptase (Bio-Rad 1708891). The cDNA was then amplified using the SYBR Premix (iScript Advanced cDNA Synthesis Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, catalog #1708891). TaqMan primers ...