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Citations for Bio-Rad :
3501 - 3550 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of each RNA sample was reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR was performed on cDNA samples using the iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was prepared from 10 to 50 ng RNA according to instructions of iScript™ cDNA Synthesis Kit (BioRad). Qiagen RNeasy® Plus mini kits (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... and quantified using the ddPCR Library Quantification Kit for Illumina TruSeq in a QX200 Droplet Digital PCR system (BioRad). Details information on the NGS protocol are described in the SI.
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated anti-rabbit and anti-mouse were used for detection using the chemiluminescence detection kit Clarity ECL (Biorad).
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR reactions were set up using the iTaq Universal Sybr Green One-Step RT-PCR kit (Biorad) according to manufacturer’s directions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using a Bio-Rad DC™ protein assay kit (Bio-Rad, Marnes-La-Coquette, France). Proteins (30 µg ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was extracted as described above and cDNA was synthesized using an iScript™ cDNA Synthesis Kit (Bio-Rad). PCR was performed using Bio-Rad CFX96 Real-Time PCR systems ...
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bioRxiv - Plant Biology 2021Quote: ... The iScript™ cDNA synthesis kit and iTaq universal SYBR green PCR master mix were obtained from Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... were detected by staining membranes with anti-mouse IFN-γ biotin (1μg/ml) (R46A2) followed by streptavidin-Alkaline Phosphatase (1μg/ml) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Microbiology 2021Quote: ... Purified RNA (500 μg) was used to synthesize cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Pre Developed Taqman® (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 8 μl sample lysate ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was then quantified by qRT-PCR using the iTaq™ Universal Probes One-Step Kit (BIO-RAD) according to manufacturer’s protocol using a forward primer (5’-CCGCTGCCCAACACAAG-3’) ...
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bioRxiv - Microbiology 2021Quote: ... and amplicons were generated via qRT-PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD) according to manufacture protocol ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from RNA using Biogen iScript kit and qPCR was performed using either Sybr Green iTaq (BioRad) or TaqMan (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... an iScript reverse transcription kit was used to convert one microgram of RNA to cDNA (Bio-Rad, Hercules, CA). No reverse transcriptase controls were included ...
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bioRxiv - Cell Biology 2022Quote: ... 1000 ng RNA was reverse-transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Bio-RAD, CA, USA). cDNA was diluted 1:5 in PCR-grade dH2O and 1.5 µl of the cDNA-mix was used per 20 µl real-time PCR reaction with 0.25 µM of each primer and 2 x FastStart Essential DNA Green Master Mix (Roche) ...
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bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions and reverse transcribed to cDNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Quantitative PCR was performed on a Bio-Rad CFX96 real-time system using SsoFast Evagreen mix (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription of 1 μg RNA/20μl was performed with the iScript™ cDNA Synthesis Kit (1708890, Bio-Rad). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (1725120 ...
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bioRxiv - Neuroscience 2021Quote: ... We used 1µg of RNA to produces cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). Primers used for RT-PCR are listed in Table 1.
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from plants using the Aurum Total RNA kit according to the manufacturer’s instructions (Bio-Rad). First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: Genomic DNA for optical mapping was extracted from agarose-embedded specimens using the CHEF Genomic Plug DNA kit (BioRad) in order to minimise fragmentation ...
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bioRxiv - Plant Biology 2021Quote: ... 1μg of total RNA were used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-rad Laboratories, India). For quantitative real-time PCR (qRT-PCR) ...
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of all fractions was quantified using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: cDNA was synthesized from 0.5 μg of total RNA using the iScript Advanced cDNA synthesis kit (Bio-Rad; 1725037) following the protocol 25°C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... and loaded onto 10% SDS-PAGE gel (TGX™ Fast Cast™ Acrylamide Kit 10%, BioRad, California, United States). Proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ semi-liquid rapid transfer system (BioRad) ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized from total RNA (0.1 – 1.0 µg) with iScript Advanced cDNA Synthesis Kit (BioRad, Hercules, CA, USA). QRT-PCR was performed using PowerUp SYBR Green Master Mix reagent (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: The single nucleus ATAC-seq experiment was performed using the SureCell ATAC-Seq Library Prep Kit (Bio-Rad, 17004620) following the manufacturer’s protocol for the OMNI-ATAC version (45) ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... The protein lysate concentration was quantified using Pierce BCA Protein Assay Kit (ThermoScientific) and10μg of soluble protein lysates were separated on a 4-12% Bis-Tris gel (BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2020Quote: ... digital droplet PCR (ddPCR) was performed using the One-step RT-ddPCR advanced kit for probes (Bio-Rad, #1864022). The final 20 μL reaction mix contained ...
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bioRxiv - Molecular Biology 2020Quote: ... Complementary DNA was synthesized from 1 μg total RNA with an iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). A CFX 96 Connect™ system (Bio-Rad ...
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bioRxiv - Systems Biology 2021Quote: ... 1μg of purified RNA was used to make cDNA using the iScript™ cDNA synthesis kit (Bio-Rad, USA) using the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... or 15% SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes using Trans-Blot Turbo RTA Mini 0.45μM LF PVDF Transfer Kit (Bio-Rad). Membranes were incubated for 1h at room temperature with blocking buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Digestion of genomic DNA was carried out using Turbo DNase treatment (TURBO DNA-free™ Kit, Bio-Rad, USA) per the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Physiology 2021Quote: ... Same amount of total RNA was used for reverse transcription by using iScript™ cDNA Synthesis Kit (Bio-Rad). RT-qPCR was done in a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... The cDNA for each sample was reverse-transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA) from 1 □g of input RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA synthesis kit (BIORAD, CAT#1708891). Quantitative real-time PCR reactions were set up in technical triplicates with Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... One microgram of extracted RNA was reverse transcribed into cDNA using iScript cDNA Synthesis Kit (BIO-RAD, Cat #1708890). Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward ...
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bioRxiv - Cell Biology 2021Quote: ... each sample was used for the cDNA synthesis carried out by the iScript cDNA synthesis kit (BioRAD, CA, USA). The real-time PCR reactions were performed by the KAPA SYBR FAST qPCR Master Mix Kit (KAPABIOSYSTEMS ...
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bioRxiv - Cancer Biology 2021Quote: ... The RNA samples (1μg) were thereafter reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, 1708890) according to the accompanying instructions ...
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bioRxiv - Microbiology 2021Quote: ... the RNA was transcribed into cDNA using the iScript™ Select cDNA Synthesis Kit with random primers (Biorad, Germany). The amount of speC transcripts was quantified using primers 29 and 30 (Tab ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were developed using Chemiluminescent Substrate kit (Pierce) and visualized using ChemiDoc XRS+ system (Bio-Rad, Hercules, CA, USA). All densitometric bands were quantified using ImageJ (NIH).
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bioRxiv - Immunology 2021Quote: ... 100ng-1μg RNA was used to synthesize cDNA by reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad). Quantitative PCR reactions were run with cDNA template in the presence of 0.625μM forward and reverse primer and 1x solution of iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... 1 ug of mRNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA). RNA was hydrolyzed and re-suspended in nuclease free water ...
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bioRxiv - Immunology 2020Quote: cDNA was synthesized from the indicated tissue RNA using iScript cDNA synthesis kit (#170-8891) acquired from Bio-Rad according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2020Quote: Cytokine concentrations in the serum of mice were determined using a Bio-Plex Pro assay kit (Bio-Rad Laboratories). A total of 50 μl of antibody-conjugated beads was added to the assay plate ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysate was spun down at 14000 rpm and protein concentration was determined using the Bradford assay as detailed in the manufacturer’s kit (Biorad). Samples were denatured with SDS and methylene blue ...
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bioRxiv - Cell Biology 2021Quote: ... and total cellular protein content was determined using the DC Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA). Then ...