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Citations for Bio-Rad :
3401 - 3450 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Total cDNA was synthesized as per the manufacturer’s instructions using the iScript cDNA synthesis kit (Bio-Rad, USA). For expression analysis using real-time PCR ...
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bioRxiv - Cell Biology 2023Quote: Knockdown was validated by qPCR analysis: RNA was isolated using the Aurum Total RNA Mini Kit (Bio-Rad). RNA yield and purity were verified by absorbance at 260/280 nm (NanoVUE spectrophotometer ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was generated from 7.5 μg of RNA using the iScript™ Advanced cDNA Synthesis Kit (Biorad, 1725038), and 20-30 ng of cDNA was then used for quantitative PCR with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: 1000 ng of DNAse-treated RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad, #1708891). This cDNA was then diluted 1:3 using ultrapure ...
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bioRxiv - Cancer Biology 2023Quote: ... 1000 ng of DNAse-treated RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad, #1708891). This cDNA was then diluted 1:3 using ultrapure ...
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bioRxiv - Physiology 2023Quote: ... The Numb immunoprecipitated material was analyzed by SDS-PAGE and gels stained with silver stain kit (Bio-Rad)
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bioRxiv - Neuroscience 2023Quote: ... and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad). Membranes were blocked for 30Lmin in 5% low-fat milk in TBS-Tween 20 0.1% at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®). Transfer and equal loading were evaluated by activation of stain-free gels by UV light ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcriptase-PCRs (RT-PCR) were carried out using the iScript RT Kit for cDNA synthesis (BioRad, 1708841). Where the final mix include 1000ng of RNA sample and 5µL of iScript RT-PCR mix with reverse transcriptase ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cDNA was reverse transcribed from 500ng of RNA using iScript IV VILO cDNA synthesis kit (Bio-Rad), with reactions incubated at 25°C for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... They were loaded in 10% acrylamide gels (TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10%, Biorad), with protein ladder (NZYColour Protein Marker II ...
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bioRxiv - Pathology 2024Quote: ... Total RNA was transcribed reversely to cDNA using iScript™ cDNA Synthesis kits (Cat # 170-8891; Bio-Rad). Quantitative PCR was performed to quantify Lrp2 mRNA abundance in the kidney using SsoFast™ EvaGreen® Supermix kits (Cat # 172-5204 ...
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bioRxiv - Molecular Biology 2024Quote: ... HMGA2 fibroid (HMGA2F) expression was determined by RT-qPCR using the iScript Advanced cDNA Synthesis Kit (BioRad, #1725037) for the reverse transcription and HMGA2 PrimePCR primer (BioRad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The quantification of mRNA levels was conducted via an iTaq Universal SYBR Green One-Step Kit (Bio-Rad). The assay was performed on an ABI StepOnePlus PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... We assessed library quantities using digital droplet PCR (ddPCR) with the Library Quantification Kit for Illumina TruSeq (BioRad). We diluted libraries to 2nM and performed paired-end sequencing using a HiSeq 2500 (Illumina).
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bioRxiv - Immunology 2024Quote: ... 1 ug of total RNA was reverse transcribed into cDNA (iScript cDNA synthesis kit, Bio-Rad cat# 1708840). cDNA was diluted 1:30 in qPCR experiments ...
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bioRxiv - Genomics 2024Quote: ... chr10:28,469,205-28,662,312 in EquCab3.0) were extracted from 10 ml bacterial cultures with the Quantum Prep Plasmid miniprep kit (BioRad), according to supplier instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred to ethanol-activated 0.2μm PVDF membranes using the Trans-Blot Turbo RTA Mini Transfer kit (#1704272) from BioRad. Each blot was blocked using 5%/0.1% BSA/Tween in TBS and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was generated using 1000 ng of total purified RNA following iScript cDNA synthesis kit (1708890, Bio-Rad) manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: RNA was extracted using the OMEGA total RNA kit and reverse transcribed using iScript reverse transcriptase (Bio-Rad). qPCR was performed using TB Green Premix Ex Taq (Takara) ...
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bioRxiv - Plant Biology 2024Quote: ... Signal detection and quantification was achieved using the Clarity Western ECL substrate kit (Bio-Rad, Catalogue No.: 1705061), a ChemiDoc MP imaging system (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The total RNA was used in generation of cDNA using a reverse transcription kit (Bio-Rad, Hercules, CA) with gene-specific anti-sense primers ...
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bioRxiv - Plant Biology 2024Quote: ... Selaginella moellendorffii total RNA was prepared from leaf midriff tissue using Aurum RNA extraction kit (Bio-Rad Laboratories) and double stranded cDNA was made with iScript cDNA synthesis kit (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2024Quote: ... protein concentrations were determined using the Bio-Rad DCTM protein assay kit (Bio-rad, Marnes-La-Coquette, France). Protein extracts (100 μg ...
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bioRxiv - Immunology 2024Quote: ... iScript™ cDNA Synthesis Kit (1708891) and iQ™ SYBR® Green Supermix (1708882) were from Bio-Rad Laboratories (CA ...
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bioRxiv - Plant Biology 2024Quote: ... The chemiluminescence-based detection was performed by using the Clarity™ Western ECL substrate detection kit (Bio-Rad) in accordance with (Giovannoni ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 µg of RNA was used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from two-week-old plants using the Aurum Total RNA Mini Kit (Bio-Rad). Reverse transcription of 1 μg of total RNA was carried out using the iScript Reverse Transcription Supermix for RT-PCR according to the manufacturer’s instructions (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... and protein concentration was determined by the DC Protein Assay Kit (Bio-Rad, based on the Lowry assay). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentration of the protein samples was calculated by using DC protein assay kit II (Biorad 500-0112).
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bioRxiv - Molecular Biology 2024Quote: ... 25 ng per sample of RNA was reverse transcribed using the iScript Reverse Transcriptase kit (BioRad, Hercules CA). cDNA was amplified within 24 hours of reverse transcription using iQ SYBR Green Supermix (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... DNase treatment was performed prior to cDNA synthesis with iScript gDNA Clear cDNA synthesis Kit (Bio-Rad, #1725035). Following this ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were then determined with the DC protein assay kit (Bio-Rad, Hercules, CA, USA, cat #: 5000121) and absorbance readings at 700 nm using a microplate spectrophotometer (Biotek Synergy H1 hybrid reader ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was synthesized from 1 μg of total RNA using iScript Advanced cDNA Synthesis Kit (Bio-Rad, 1708890). Real time PCR was performed using a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were further lysed using the lysis buffer of Aurum™ Total RNA Mini Kit (Bio-Rad, #7326820). Lysates were passed 10 times through a sterile BD Microlance™ 3 needle (#300635 ...
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bioRxiv - Immunology 2024Quote: ... One microgram of RNA per sample was reverse transcribed into cDNA using Advanced iScript cDNA synthesis kit (BioRad). cDNA was then amplified using murine gene-specific primers (Supplementary Table ...
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bioRxiv - Immunology 2024Quote: ... 0.5 μg of the isolated RNA was used for cDNA synthesis with iScript cDNA Synthesis Kit (Bio-Rad). Real-time quantitative PCR was performed with iTaq Universal SYBR® Green Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of RNA was retro transcribed using the iScript cDNA Synthesis Kit and polydT primers (Bio-Rad).
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bioRxiv - Immunology 2024Quote: ... cDNA was prepared by reverse transcription of extracted RNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories), and qPCR of cDNA was performed using the KAPA SYBR FAST qPCR Kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... One ug of RNA was used for synthesis of complementary DNA (cDNA) by reverse transcription kit (Bio-Rad). The sequence extracted from Ensembl database was utilized to design primer sets for real-time PCR ...
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bioRxiv - Cell Biology 2024Quote: ... The separated proteins were blotted on nitrocellulose membranes using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized from 1 μg of total RNA using the iScriptTM cDNA synthesis kit (#1708891, Bio-Rad). PowerUp SYBR Green Master Mix (Applied Biosystems #A25776) ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of protein lysates was measured by Bradford assay using the BioRad protein detection kit (Bio-Rad). Typically ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three further times before being developed using a BioRad ECL Chemiluminescence Kit (Bio-Rad Laboratories) and imaged using a BioRad imager using Image Lab software.
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bioRxiv - Biochemistry 2024Quote: ... cDNA was synthesized from 1 μg of total RNA using iScript Advanced cDNA Synthesis Kit (Bio-Rad, 1708890). Real time PCR was performed using a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... One microgram of total RNA was employed for cDNA synthesis with the iScript cDNA synthesis kit (Bio-Rad). The resulting cDNA was diluted fivefold ...
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bioRxiv - Genetics 2024Quote: ... the signals were detected with PDS standard ECL GeneSpin kit using the ChemiDoc Touch Imaging System (Bio-Rad). After S9.6 signal detection ...
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bioRxiv - Developmental Biology 2024Quote: ... 1µg of total RNA was used for generation of cDNA (iScript cDNA synthesis kit, 170–8891, BioRad, USA). For quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of RNA was subjected to reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad; 1708891) and diluted 1:5 in molecular grade water (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®). Transfer and equal loading were evaluated by activation of stain-free gels by UV light ...