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Citations for Bio-Rad :
3451 - 3500 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 8 µl sample lysate ...
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bioRxiv - Cancer Biology 2021Quote: ... Messenger RNA (mRNA) was converted to a single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad). Q-PCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein quantification was assessed using the BCA Protein Assay Kit (Pierce) or the RC DC Protein Assay (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Messenger RNA (mRNA) was converted to a single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad). qPCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... Molarity of the pool was quantified by qPCR using KAPA Library Quantification Kit and the CFX96 qPCR system (Biorad) before sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant were transferred to fresh tube and solubilized protein was measured using a DC protein assay kit (Bio-Rad). For immunoblotting an appropriate volume of 1 x Laemmli (Bio-rad ...
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bioRxiv - Cell Biology 2020Quote: ... Synthesis of complementary DNA (cDNA) was completed using 1 μg of RNA and the iScript cDNA synthesis kit (BioRad). Each qRT-PCR was performed as triplicate reactions with the following reaction mixture ...
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bioRxiv - Immunology 2021Quote: ... Approximately 400 pg of RNA was subjected to reverse transcription using the iScript RT kit (BioRad, Inc., Hercules, CA). Resulting cDNA products were included with 50nM VH1-VH6 specific primers and 250nM Ca ...
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bioRxiv - Microbiology 2021Quote: ... and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat. # 1708890, Bio-Rad). Real-time qPCR was conducted using the iTaq™ Universal SYBR® GreenSupermix (Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 0.5 to 1μg RNA using SensiFast cDNA synthesis kit and qRT-PCR was performed using iTaq Universal SYBR Green Supermix (Biorad) (refer Purushothaman et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNAs were extracted by the Aurum ™ Total RNA mini kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subject to qRT-PCR assays by the Power SYBR® Green RNA-to-Ct™ 1-Step Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA synthesis was carried out using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA Cat# 1708890) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of total RNA was reverse transcribed with random primers using an iScript gClear cDNA synthesis kit (BioRad) and including a no-RT and controls for each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs was prepared using RevertAid First Strand cDNA Synthesis Kit (Thermoscientific) according to the manufacturer’s recommendation to perform qRT-PCR (BioRad) (Sup Table 1) ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from REF subclones 2c and 11b using the Aurum Total RNA Mini Kit (Bio-rad) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad, 170-4270). After blocking with 5 % fat-free milk in PBS-Tween 20 ...
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bioRxiv - Genetics 2021Quote: ... Chemiluminescence detection was performed according to the manufacturer’s instructions (Amersham ECL Western Blotting Detection Reagent kit) followed by exposure using Chemidoc gel-documentation system (BioRad). For imaging using Li-Cor ...
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bioRxiv - Genetics 2021Quote: ... normalized to 10ng/μL and then reverse transcribed into cDNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The cDNA was used as template for the droplet digital PCR (ddPCR ...
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bioRxiv - Cancer Biology 2021Quote: ... Signals were developed using Amersham ECL Western Blotting Detection Kit (Cytiva, # RPN2108) and imaged on a Chemidoc Touch (Biorad). Relative protein quantitation was calculated based on normalized integrated intensity.
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bioRxiv - Immunology 2021Quote: RNA was isolated using QIAGEN RNeasy Mini Kit and cDNA was synthesized using the iScript Reverse Transcription Supermix (Biorad). Quantitative real-time (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... the samples were transferred onto a membrane using Trans-Blot® TurboTM RTA transfer system and kit (Bio-Rad). The membranes were saturated with 5% skimmed milk ...
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bioRxiv - Cell Biology 2021Quote: ... RNA (1 μg) was reverse transcribed into cDNA using iScript Reverse Transcription Supermix Kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared to the control gene 18S were determined by quantitative real-time PCR using SYBER Green PCR Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 ug) was converted to cDNA using the iScriptTM cDNA synthesis kit (# 1708891, BIO-RAD, Hercules, CA) following the manufacturer’s protocol (BIO-RAD ...
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bioRxiv - Bioengineering 2021Quote: ... The total RNA was then used to synthesize 200 ng of cDNA using the iScript Synthesis Kit (Biorad, #1708890) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were then used to isolate the total RNA using the Aurum Total RNA Mini Kit (Biorad, #7326820) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 1μg of RNA for each sample was used to make cDNA using the iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNA was synthesized using equal quantities of RNA (200 ng) with iScript Reverse Transcription kit (Bio-Rad Laboratories®).
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bioRxiv - Neuroscience 2020Quote: ... 500 ng of RNA was retrotranscribed to single-stranded cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891). The analyses were performed in 3 animals/group ...
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bioRxiv - Cell Biology 2020Quote: ... and quantified by real-time PCR using the IQ SYBR green supermix kit (Bio-Rad Laboratories, Hercules, CA, USA) and the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... 600ng of RNA was reverse-transcribed (RT) to generate cDNA using iScript reverse transcription kit (Biorad, ref. 170-8841), following manufacturer’s instruction ...
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bioRxiv - Plant Biology 2021Quote: ... DNase treated with Ambion Turbo DNase (AM1907) and cDNA was synthesized with the iScript cDNA synthesis kit (Bio-Rad). Real-time RT-PCR was performed with the iQ SYBR Green Supermix from Bio-Rad with a standard 2-step program of 40 cycles ...
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bioRxiv - Cell Biology 2021Quote: Protein concentrations were determined by use of the Bradford assay (Bio-Rad protein assay kit II, Cat. No. 5000002). For immunoblotting ...
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bioRxiv - Cell Biology 2021Quote: ... Chemiluminescence detection was performed according to the manufacturer’s instructions (Amersham ECL Western Blotting Detection Reagent kit) followed by exposure using Chemidoc gel-documentation system (BioRad). For imaging using Li-Cor ...
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bioRxiv - Plant Biology 2020Quote: ... at a 1:10,000 dilution and we developed blots using the AP conjugate substrate kit (Bio-Rad; Hercules, USA). We used polyclonal GFP antibodies (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2021Quote: ... and cDNA synthesis was performed on 900 ng DNased RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated with 300ng of total RNA using iScript™ Select cDNA Synthesis Kit (Bio-Rad, Cat# 1708897) with the following conditions (25 °C for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... PCR reaction mixtures were as follows: 5.5 μl One-Step RT-ddPCR Advanced Kit for Probes Supermix (no dUTP’s) (Bio-Rad), 2.2 μl reverse transcriptase ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was isolated as described above with cDNA prepared using the iScript cDNA synthesis kit (Bio-Rad #1708890) and qPCR conditions previously described [19] ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated with 300ng of total RNA using iScript™ Select cDNA Synthesis Kit (Bio-Rad, Cat# 1708897) with the following conditions (25 °C for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was collected and protein concentration was determined using the Bio-Rad protein assay kit (BIORAD, Hercules, USA) and bovine serum albumin as a standard ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted and qRT-PCR was performed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA) and 1000 ng of total RNA according to the manufacturer’s instructions as previously described (64) ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed using using the SYBR Green kit (iTaq Universal SYBR Green Supermix, BIO-RAD, 1725124) on a BIO-RAD CFX384 ...
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bioRxiv - Microbiology 2020Quote: ... Immunoreactive bands were detected using an ECL detection kit (EzWestLumi plus, ATTO) with a ChemiDoc imaging system (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... About 500-1000ng RNA was used as a template for cDNA synthesis using a cDNA synthesis kit from Biorad. The cDNA was further diluted to 1:10 with nuclease-free water ...
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bioRxiv - Cell Biology 2021Quote: ... signals were obtained with peroxidase-conjugated secondary antibodies (Cell Signalling Technology) and revealed with Clarity Western ECL kit (Biorad) and a ChemiDoc ™ XRS Imaging system (BioRad).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kit and BioRad chemiluminiscence machine scanner.The densitometry analysis of immunoblot images was performed using quantity One Software(Bio-Rad). Densitometry values were normalzed to the loading control(Gapdh or Actin ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The total RNA extracted was reverse transcribed using the iScript™ cDNA Synthesis kit (Bio-Rad Laboratories, CA, USA) with a thermal profile ...
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bioRxiv - Neuroscience 2022Quote: ... 300-400 ng of cDNA from VP was synthesized using the reverse transcriptase iScript cDNA synthesis kit (Bio-Rad). mRNA expression changes were measured using qRT- PCR with PerfeCTa SYBR Green FastMix (Quanta) ...