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Citations for Bio-Rad :
2951 - 3000 of 3545 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were analyzed with SDS–PAGE using 4-15% Mini-PROTEAN TGX Stain-Free Gels (4568083 Bio-Rad). Proteins were transferred to a nitrocellulose membrane (1704271 ...
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bioRxiv - Microbiology 2024Quote: Total bacterial DNA was extracted from LB broth cell pellets by InstaGene Matrix (Bio-Rad, Marnes-la-Coquette, France), and real-time ZKIR PCR was performed using Rotor-Gene Q (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto a polyvinyldiene fluoride (PVDF) membrane in a Mini Trans-Blot cell (Bio-Rad, CA, USA) at a constant current of 315 mA on ice for 90 min ...
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Genetics 2024Quote: ... were loaded and run on a mini gel system (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad REF-1658004) at 200V for 35-45 minutes ...
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bioRxiv - Immunology 2024Quote: ... pre-soaked in transfer buffer (20 mM Tris, 190 mM glycine, 20% Methanol, pH 8.3) using a Mini-Transfer cell (BioRad) for 1.5 hours (100 V constant ...
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bioRxiv - Bioengineering 2020Quote: ... A 10 μL cell-free sample was injected into a BioRad chromatographic column (Aminex HPX-87H, 7.8 mm 300 mm, Biorad, USA). The HPLC was equipped with a P680 pump ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined at indicated time points by adding 1/10 volume AlamarBlue solution (Biorad, Hercules, CA, United States) to each well ...
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bioRxiv - Systems Biology 2020Quote: ... 20 µg of each extract was analyzed by electrophoresis in 10% polyacrylamide gels and transferred to polyvinylidene difluoride membranes using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Biorad). After 1 h incubation at room temperature in Odyssey Blocking Buffer (TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... whole cell lysates were separated on 8% sodium dodecyl sulfate (SDS) polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (BioRad). The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The measurements were performed using the blue laser (488 nm) of the S3e Cell Sorter (Bio-Rad GmbH; Munich, Germany). The cell cycle phases were determined with the FlowJo (FlowJo ...
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bioRxiv - Immunology 2021Quote: ... samples were transferred to FACS polystyrene tubes using a disposable bacterial filter (50 μm, Sysmex) and macrophages were sorted into 100 μL of PBS using a S3TM Cell Sorter (BioRad). Isolated cells were verified by fluorescence microscopy and differential interference contrast.
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bioRxiv - Developmental Biology 2020Quote: ... and electrophoresed at 100-200 V for one to two hours in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (BIORAD). Proteins were transferred onto PVDF membranes (TransBlot Turbo Mini PVDF Transfer Packs ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... supplemented with 210 mM NaCl) to 200 µL of cell suspension and transferring into 0.4 cm gap electroporation cuvettes (Bio-Rad). The cells were electroporated at 350 µF/400 V using a Bio-Rad Gene Pulser II electroporation system ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Molecular Biology 2020Quote: ... cell viability was determined by measuring the absorbance at 490 nm using a 550 BioRad platereader (Bio-Rad, Hertfordshire, UK). At least triplicates were done for each condition to get statistical significance ...
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bioRxiv - Molecular Biology 2020Quote: ... ½ of a confluent T75 flask of WT F1 2-1 MS2129 female ESCs were electroporated with 40μg of PciI linearized PCR2.1-Puro XistΔE targeting plasmid (800v, 0.2ms, 4mm cuvette, Biorad X-Cell electroporation module) and plated at varying dilutions on 10cm plates of confluent irradiated DR4 feeders ...
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bioRxiv - Molecular Biology 2020Quote: ... were electroporated with 40μg of Not1 linearized paa2Δ1.7 plasmid DNA (800v, 0.2ms, 4mm cuvette using a Biorad X-Cell electroporation machine) and plated on confluent irradiated DR4 feeders ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were loaded at 100000 cells/lane and separated by electrophoresis under denaturing conditions using the Mini-Protean IITM system (BioRad) for 50 minutes at 180 V ...
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bioRxiv - Molecular Biology 2020Quote: Whole-cell lysates were run on 10 or 15% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (1620097, Bio-Rad) by standard methods ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cell were lysed with modified RIPA buffer and EP300 was co-immunoprecipitated using magnetic Sure-bead Protein G (Bio-Rad)-coupled p300 (clone 2033 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA from the barcoded cells for each sample was subsequently reverse-transcribed in a C1000 Touch Thermal cycler (Bio-Rad) and all subsequent steps to generate single-cell libraries were performed according to the manufacturer’s protocol with no modifications ...
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bioRxiv - Plant Biology 2019Quote: ... The embedded cells were then lysed and DNA was separated using a CHEF Mapper® XA System (BioRad, Munich, Germany) essentially as previously described (Swiatek et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...
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bioRxiv - Cancer Biology 2019Quote: ... total RNA was extracted from dissociated single cells using QIAGEN RNease Mini Kit and cDNA was prepared using 1µg RNA with iScript cDNA Synthesis Kit (Bio-Rad). SYBR green-based QPCR was performed using murine primers listed as below (GLI1 ...
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bioRxiv - Immunology 2019Quote: ... Donor CD103+ KLRG1-CD8 T cells from LP of transplanted tissue were identified following the gating strategy in FigureS11 and single cells were sorted into 96-well plates (BioRad) containing 5 µL capture buffer (20 mM Tris-HCl pH8 ...
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Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cellsbioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4– 15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and 25-30 μg of protein were loaded on a 7.5% separating gel using Mini Trans-Blot Cell (Bio-Rad). Nitrocellulose membranes were stained with primary antibodies ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h and then incubated with primary overnight and secondary antibodies for 2 h ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Microbiology 2019Quote: Approximately 1 × 105 bacterial cells were suspended in PBS containing 1% (w/v) PBS-BSA and incubated with fH (Biorad) at a final concentration of 5 μg/mL for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h in nonfat-milk ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 2-10 μl of unmethylated DNA (>50 ng/μl) was added to cells and transferred to a 0.4 cm gap electroporation cuvette (Bio-Rad). Cells were exposed to 2.5kV for 4-6 msec ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in 1X PBS and mechanically lysed by sonication before protein standardized by DC assay (BIO-RAD) and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR) ...
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bioRxiv - Plant Biology 2020Quote: Transient expression in onion cells (bulb sliced to ~16 cm2) was conducted using the Biolistic PDS 1000/He Particle Delivery System (Biorad) (1100 psi ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: ... DGF-298 electrocompetent cells were transformed with 300 ng of the purified PCR product (described above) by electroporation in a Genepulser XCell (Biorad) at 1800 V ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were separated by SDS-PAGE using a Mini-Protein Tera Cell system (Bio-Rad Laboratories, Inc., Hercules, USA, CA). Proteins were visualized by CBB staining as described elsewhere.
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bioRxiv - Biochemistry 2020Quote: ... by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany). Proteins were transferred to a nitrocellulose membrane (Millipore ...
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bioRxiv - Neuroscience 2021Quote: MV pellets were sonicated three times for 10 s at 20 Hz (Vibra cell VCX130) in 2% SDS and heated at 56°C in Laemmli loading buffer (Biorad). The protein content was measured using the Pierce 660 nm protein assay kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The quantification of protein content in exosome suspensions and whole-cell lysates was undertaken by a modified Lowry assay (DC Protein Assay, BIORAD). For immunoblotting ...
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bioRxiv - Cancer Biology 2021Quote: ... 48 cytokines were quantified in the cell supernatant using a Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad, #12007283) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... based on the manufacturer’s protocol and 30 μg of protein lysate of each sample was separated by electrophoresis in 7.5% or 12% SDS-PAGE gel and transferred into 0.45 μm PVDF transfer membrane using a Semi-Dry transfer cell (Trans-Blot SD, Bio-Rad). After transfer ...
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bioRxiv - Cancer Biology 2019Quote: RNA was isolated from cell lines or flash-frozen tissue as described above was reverse-transcribed using iScript (Bio-Rad). Real-Time PCR was conducted using VeriQuest SYBR Green qPCR Master Mix (Affymetrix) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were separated by 10% SDS-PAGE followed by electrophoretic transfer to polyvinylidene difluoride membranes (Bio-Rad, Hercules CA). Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween 20 and incubated at 4°C overnight with the TRPM4 antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were Western blotted overnight with constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA). Membranes were destained from residual Coomassie traces by washes in methanol for several minutes ...