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Citations for Bio-Rad :
3001 - 3050 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was denatured with 1:1 Laemlli loading buffer (BIORAD) by boiling for 10 minutes on a dry bath (Thermolyne Dri-Bath ...
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bioRxiv - Biochemistry 2023Quote: ... The collected cells were mixed 1:1 with TrypanBlue (BioRad) and counted.
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bioRxiv - Genetics 2023Quote: ... 1:500 to 1:1000 anti-rabbit (BioRad, 172-1019). Signal was detected using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... (BioRad Cl:A3-1) and goat anti-rat IgG cross-absorbed Alexa Fluor® 488 secondary antibody (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts (1 mg) of protein were diluted 1:1 in sample loading buffer (2x, Bio-Rad, UK) for each extraction method and loaded onto a 12% Tris-glycine pre-cast SDS gel (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Sigma Aldrich respectively) and Sca-1 (1:100, Bio-Rad, MCA2782) at room temperature to detect M2 macrophages ...
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bioRxiv - Neuroscience 2021Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 5% milk/PBST at RT ...
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bioRxiv - Cell Biology 2020Quote: ... and 1:500 to 1:1000 anti-rabbit (BioRad, 172–1019). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and F4/80 (dilution 1:200, clone A3-1, Bio-Rad); ER2 EDTA for CD3 (dilution 1:100 ...
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bioRxiv - Molecular Biology 2019Quote: ... diluted 1:3000 (or 1:4000 goat anti-mouse (Bio-Rad) for α-tubulin ...
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bioRxiv - Neuroscience 2019Quote: ... Sheep anti-GFP (Bio-Rad, 4745-1051, 1:250-1:1000), Chicken anti-GFP (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 0.1% Tween-20 in 1x PBS (PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-Gapdh antibody, BioRad) in 5% milk/PBST at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were mixed 1:1 with 2x Laemmli sample buffer (BioRad) supplemented with 5% □-ME (Sigma ...
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bioRxiv - Genetics 2023Quote: ... and mixed 1:1 with 2X Sample Loading Buffer (Bio-Rad) + 5% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were diluted 1:1 with 2× Laemmli buffer (Bio-Rad) supplemented with 54 mg ml-1 DTT ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for 1 hour in 1% casein (Bio-Rad) in PBST (0.1% Tween-20 in PBS) ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Cancer Biology 2021Quote: ... The same amounts of protein extracts were subjected to polyacrylamide gel electrophoresis using the 4% to 20% Mini-Protean TGX gel system (Bio-Rad), transferred to PVDF (0.45 μm pore size ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples from LNCaP cells used to measure luciferase activity (described in Fig. 4A) were loaded into 4% to 15% SDS-PAGE (Bio-Rad) and subjected to electrophoretic analysis and subsequent blotting ...
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bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... 1.5 μl for bound franction for GFP detection) were loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Protein samples were heated at 55°C for 10 min and 10 μl was loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was transferred to new tubes and 20 µL of the sample were loaded onto a 4-20 % gradient SDS-PAGE gel (Bio-Rad) and analyzed by immunoblotting.
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bioRxiv - Immunology 2021Quote: ... They were incubated at the room temperature for 5-10 min and they were placed inside a 4-mm cuvette (Bio-Rad). The cuvettes were pulsed using the BTX electroporator (300 V ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad, Hercules, CA), and then transferred to PVDF membranes ...
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bioRxiv - Genetics 2021Quote: ... 150 μg of protein was then separated on Criterion Precast Gradient 4-15% SDS-PAGE gels (Bio-Rad, cat. number 5671083) and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution ...
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 5 minutes at 95°C before separation on a gradient 4-20% SDS-PAGE gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semi-dry transfer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Ten to twenty micrograms of denatured protein was loaded onto a 4-20% TGX stain-free polyacrylamide gel (Bio-Rad, USA) or NuPAGE 3-8% Tris acetate gel and subsequently transferred onto pre-cut nitrocellulose membranes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... SDS PAGE was performed using 7.5% or 4-20% Mini-PROTEAN® TGX™ precast protein gels (BIO-RAD, 4561023/4561094) run in running buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... 15µg of each sample was loaded per lane of a MIDI 4-15% Criterion Precast Midi SDS page gel (BioRad #5671085) and electrophoresis was performed at 120mV for 75 minutes ...
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bioRxiv - Developmental Biology 2020Quote: Equal amounts of protein were resolved on either a 15% or a 4-20% Tris-glycine gradient SDS-PAGE gel and transferred to a polyvinylidene difluoride Immobilon-FL membrane (PVDF; Bio-rad). Membranes were blocked for 30 min at room temperature with 5% BSA in TBST (50 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... 15-25 μg of proteins were loaded onto 4-12% Mini-PROTEAN Tris-Tricine Precast Gels or Criterion Tris-Tricine Precast Gels (Bio-Rad), and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science) ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was then added and the solution was mixed at 4°C for 10 minutes before addition of 100 mg of Biobeads SM2 (Bio-Rad). Biobeads were washed into methanol ...
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bioRxiv - Molecular Biology 2020Quote: ... aliquots of the supernatant and resuspended pellet fractions were separated by SDS-PAGE using 4-15% gradient TGX gels (Bio-Rad) and analyzed by densitometry using Image Lab software (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins associated with half of the Dynabeads were eluted with 22 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) by boiling at 95 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The α-FN-EDA Fab was verified as >90% pure by SDS-PAGE (performed on 4-20% gradient gels (Bio-Rad)).
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bioRxiv - Microbiology 2020Quote: ... concentration of total aerobic GNB and of MDR-GNB were determined by plating serial dilutions (pure, 10−2, 10−4) of initial faeces or EA sample onto Drigalski agar (Bio-Rad) with or without 1mg/L cefotaxime ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... were separated by SDS PAGE in 4-12% Bolt Bis-Tris Plus gels (ThermoFischer Scientific) and transferred to 0.2 µm Immun-Blot® PVDF membrane (BIO-RAD). After blocking with 5% (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... The denatured samples were resolved on a 4-20% Mini-PROTEAN TGX Stain-Free precast protein gel (Bio-Rad, cat# 4568094) and transferred to a nitrocellulose membrane (0.45 μm ...
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bioRxiv - Neuroscience 2019Quote: ... Protein samples were electrophoresed on NovexTM 4-20% Tris-Glycine gels (Thermo-Fisher) and transferred onto nitrocellulose membrane using the CriterionTM Blotter transfer system (Bio-Rad). Membranes were blocked in 5% milk in .1% Tween/Tris-buffered saline (TBST ...