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Citations for Bio-Rad :
2851 - 2900 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation at 10,000xg for 10 minutes at 4°C and then boiled in 2x Laemmli Sample Buffer (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: Eluted samples were resolved by SDS-PAGE using 4-20% gradient gels and then transferred to the nitrocellulose membrane using the Trans-Blot Turbo transfer system (Biorad). Following the blocking for 1 hour in blocking buffer (5% milk in TBST) ...
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bioRxiv - Biochemistry 2021Quote: ... two probes (250 nM final each) (Supplementary Table 4) and template DNA were combined with ddPCR Supermix for Probes (No dUTP) (BioRad) in a final 20 μL reaction volume ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μg of protein was loaded on 4-20% Mini-PROTEAN TGS Precast Protein Gels (Cat. No. 4561094, Bio-Rad) from each condition and ran at 100 V for 1 hour followed by transferring the protein on a PVDF membrane ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were then loaded (15-20 μg of protein per well) in a 4–20% Precast Protein Gel (Biorad) and resolved by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated according to size by SDS-PAGE in a Mini-Protean TGX stain-free 4-20% gradient gel (BioRad) and thereafter transferred onto a PVDF membrane ...
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bioRxiv - Physiology 2021Quote: ... and proteins were stripped from beads by incubation in gel loading buffer with beta-mercaptoethanol and loaded to 4-15 % gels (Criterion; BioRad) for SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... and electroporated (2500 V, 200 Ω, 25 μF, exponential decay, time constant ~ 4 ms) using Gene Pulser Xcell (Bio-Rad). 1 mL warm BHI medium was added and the cells were incubated at 30 °C for 6 h under shaking at 200 rpm and plated on BHI agar plates with 10 μg/mL erythromycin ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 20 mg of total protein were loaded for electrophoresis into 4–20% sodium dodecyl sulfate polyacrylamide gels (Bio-Rad). Separated proteins were transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 4-15% SDS-PAGE gel (Bis-Tris Criterion™ TGX™ pre-cast gels, Bio-Rad) and transferred to PVDF membranes (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared for 10 min at 14000 rpm at 4°C and protein concentration of the supernatant was determined by using Protein Assay (BioRad). The co-immunoprecipitation was performed using GFP trap® beads (Chromotek ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoblotting was performed with 4-15% gradient Mini-PROTEAN TGX precast polyacrylamide gels and nitrocellulose membranes (Bio-Rad, Hercules, CA). Membranes were blocked with 5% milk in TBS with 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples from total or immunopurified ribosomes or aliquots from TCA precipitated sucrose gradient fractions were run in 1X Laemmli buffer on 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad) in 1X Tris/Glycine/SDS buffer (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cell lysates and 5 μl of purified viruses were diluted in Laemmli buffer and loaded on a pre-casted Criterion XT 4-12% gradient gel (Biorad). Gels were transfered to PVDF membrane using a wet-electroblotting chamber system (Biorad ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein cellular lysates (20μg) were analysed by 4-15% gradient SDS-PAGE and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were resolved using 4-12% BisTris-PAGE and transferred to a PVDF membranes (Bio-Rad catalogue number 162-0177) using a Gel Transfer Cell (BioRad catalogue number 1703930) ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... 40 μg of cell lysates was separated by 4-20% Tris-glycine MINI-PROTEAN TGX gels (Bio-Rad, 456-1094) and transferred to PVD membranes using Trans-Blot Turbo Transfer Pack (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: SDS-page gel electrophoresis analysis (12.5% running gel, 4% stacking gel stained with Coomassie brilliant blue R-250 Bio-Rad) was carried out for checking the yield of PsbS at every step of overexpression ...
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 95°C for 5 min and the transcription products were resolved on denaturing 4% polyacrylamide gel and visualized using a Personal Molecular Imager (BioRad).
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bioRxiv - Immunology 2021Quote: ... Lysates (30-50 µg) were resolved according to molecular weight by electrophoresis using Mini-PROTEAN TGX Precast gels 4-20 % gradient (BioRad). Next ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 14,000 rcf for 20 min at 4°C and protein amounts in the supernatant determined using the Bradford assay (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared in Licor protein sample loading buffer and run on TGX 7.5% and 4-20% Tris-glycine gels (Bio-Rad) and blotted onto a nitrocellulose membrane ...
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Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuolesbioRxiv - Cell Biology 2021Quote: ... The same volume of supernatant was used to separate GFP from GFP-Atg8 isoforms onto a commercial 4–20% acrylamide gradient gel (BioRad) and migrated 45 min at 100 V in 1x MES buffer ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were sonicated prior to being separated by SDS PAGE using a 4-20% Tris-glycine polyacrylamide pre-cast gel (BioRad) and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... 4-8 µL of samples and 3 µL of Precision Plus Protein Dual Color Standard (cat. no. 1610374, Bio-Rad) were run on 4-12% Bis-Tris Bolt gels (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... The same quantity of samples used for the competition assay was run on a 4%–20% gradient Tris-Glycine Gel (BioRad) and transferred to nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 μg of total protein was separated on a 4–20% SDS-PAGE gel (mini-PROTEAN TGX gels, Bio-Rad) along with PageRuler Prestained protein ladder (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were performed with 4-20% Mini-PROTEAN(R) TGX(tm) Precast Protein Gels (Bio-rad, #4561095 or #4561096) and Trans-Blot ® Turbo™ Mini PVDF Transfer Packs (Bio-rad #1704156) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged at 14,000g for 15 min at 4°C and supernatant protein concentrations were determined by Bradford assay (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... 20 μL of cell lysates were then loaded into precast 4-20% polyacrylamide SDS-PAGE gels (Bio-Rad, Catalog #: 5671094) for electrophoretic separation ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Cell Biology 2022Quote: ... 30 and 36 hpi respectively on a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (4561083, Bio-Rad). Blotting was performed using the Trans-Blot Turbo Mini 0.2 μm Nitrocellulose Transfer Pack (1704158 ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts of total protein were loaded on a 4-20% gradient SDS-PAGE gel (Mini Protean TGX, Bio-Rad), then blotted onto nitrocellulose membranes and probed with anti-villin mouse monoclonal antibody (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... and 15 μl of each was loaded on to a 4 - 15% polyacrylamide Tris/Glycine minigel (Bio-Rad, Hercules, CA) and separated at 100 V ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein was determined directly on the membrane using the 4-15% Mini-PROTEAN TGX-Stain Free Protein Gel system (BioRad). Antibodies used for Western blotting analysis were ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 20 μg of total proteins in tissue lysates were separated on electrophoresis using 4 - 12% gradient SDS-polyacrylamide gels (BioRad) and transferred to the PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were subsequently separated by electrophoresis on precast gels (4-20% mini protein TGX gels, Bio-Rad catalog #456-1094) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 60-70 μg of protein was loaded onto 7 cm pH 3-10 NL and pH 4-7 IPG strips (BioRad) and subjected to first-dimensional electrophoresis with increasing voltages from 50V to 2000V ...
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bioRxiv - Neuroscience 2022Quote: ... equal amounts of protein (2.5 or 10 µg) were separated by SDS-PAGE on 4-12% Bis-Tris or 3-8% Tris acetate gels (Criterion XT, BioRad) and transferred to nitrocellulose using TransBlot Turbo (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: Equivalent amounts of 5-15 μg of protein for gel loading (cell lysates, mitochondrial/cytoplasmic fractions) were separated by SDS/PAGE on 4-20% Tris/glycine precast gels (BioRad) and transferred to PVDF membranes (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: Three biological exosome samples (20 μg/sample) were separated on 4-15% mini-protein TGX precast protein gels (Bio-Rad) and subsequently stained with Coomassie Blue ...
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bioRxiv - Neuroscience 2022Quote: ... Regional lysates were centrifuged at 21,000 x g for 20 minutes at 4 °C and the supernatant was removed and suspended in 4X laemmli buffer (BioRad) with 2-mercaptaethanol (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... were resolved by SDS-PAGE using stain-free precast gels (4-15% Mini-Protean Tgx Stain-Free Protein Gels, Biorad). Gels were then activated by Ultraviolet light with the ChemiDocTM Touch Imaging System (Biorad ...
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bioRxiv - Physiology 2022Quote: Equal amounts of protein extracts were resolved by SDS–PAGE using 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to PVDF membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad) staining.
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...