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Citations for Bio-Rad :
2951 - 3000 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 10-50 μg protein was loaded on 10% acrylamide home-made or gradient (4-15% acrylamide) commercial (BIO-RAD, 4561086) Tris-Glycine-SDS gels along a protein ladder (PageRuler prestained protein ladder ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein per sample was resolved on a NuPage 4-12% Bis-Tris polyacrylamide mini gel (Bio-Rad) and transferred to a nitrocellulose membrane in Towbin transfer buffer containing 20% MeOH at 100V for 1 hour or 25V for 16 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... held at 4 °C) was performed in a C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad). After RT reaction ...
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bioRxiv - Genetics 2023Quote: ... Total protein (75 μg per lane) was loaded onto pre-cast 4-20% gradient Mini-PROTEAN TGX Stain-Free SDS PAGE gels (BioRad). Proteins were transferred onto nitrocellulose membrane (0.2 μm pore size ...
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bioRxiv - Biochemistry 2023Quote: ... Blue Native PAGE gel experiments were performed in a cold room (4 °C) using 10% precast gels (BioRad Mini-PROTEAN) and run at 170 V for 55 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 15 µL bound and 7 µl input sample was separated on 4-15% TGX SDS-PAGE gel (BioRad #456-1086) and transferred to PVDF membrane (Immobilon-FL ...
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bioRxiv - Cell Biology 2023Quote: ... or poly-acrylamide gels and proteins were transferred overnight at 4°C on a nitrocellulose membrane using a liquid transfer system (BioRad). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2023Quote: ... the eluted biotinylated proteins were collected and run on a 4-15% Mini-Protean TGX Precast Protein Gel (Bio-Rad) before being transferred to a nitrocellulose membrane and stained for biotin ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed by spectrophotometry with PierceTM BCA Protein Assay Kit (ThermoFischer Scientific, cat # 23225) and proteins were separated on a 4% to 20% polyacrylamide gel (Biorad), and transferred to PVDF membrane (Bio-rad ...
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bioRxiv - Immunology 2023Quote: ... SDS-PAGE was performed at 100 V on 4-15% gradient acrylamide Stain-free gels in Tris-Glycine-SDS buffer (BioRad), with 5-10 µg of protein ...
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bioRxiv - Neuroscience 2023Quote: ... 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel, #567-1085, Bio-Rad) under denaturing conditions ...
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bioRxiv - Immunology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were loaded into wells of a Mini-PROTEAN® TGX™ precast gradient (4% - 15%) gel (#4561083, Bio-Rad) and electrophoresis was run at 30 mA per gel for approximately 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were electrophoresed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis using a 4%–20% gradient polyacrylamide gel (Bio-Rad, 5678093) and transferred to 0.45-μm polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... Equivalent amounts of total protein were separated by SDS-PAGE on 4% to 15% Criterion TGX gels (Bio-Rad; 5671083). Megalin and cubilin were detected with anti-megalin antibody generously provided by Dr ...
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bioRxiv - Neuroscience 2023Quote: Proteins were separated in 4-15% Mini-PROTEAN® TGX Stain Free™ Protein gels (Bio-Rad, Hercules, CA, USA) and transferred to a nitrocellulose membrane (Hybond™-P ...
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bioRxiv - Microbiology 2023Quote: Protein samples were centrifuged (150,000 × g for 10 min at 4°C) and loaded onto an Enrich SEC 650 10 × 300 column (Bio-Rad) using the AKTA explore system (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled 5min at 90°C and 10µl was loaded on a 4-20% gradient SDS-PAGE gel (Biorad #4561096). Gel was stained with Coomassie brilliant blue R.
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... and equal amounts of protein were loaded and run on 4-20% gradient SDS-PAGE gels (4561096; Bio-Rad Laboratories). Proteins were transferred from SDS-PAGE gels to nitrocellulose membranes using Trans-Blot Turbo (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Cell Biology 2023Quote: ... Protein extracts were mixed with Laemmli sample buffer and separated by SDS-PAGE using 4-20% TGX gels (Bio-Rad) and transferred to 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... cuvette gap width 0.4 cm) with 10 µg of in vitro transcribed HCV full-length RNA using the Gene Pulser Xcell system (BioRad). Cells of two electroporations were combined in 20 ml of DMEM complete and seeded into one 15 cm dish ...
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bioRxiv - Microbiology 2023Quote: ... Denatured samples (15 µL) were separated by SDS-PAGE using pre-cast 4 - 20% gels (Mini.PROTEAN TGX Gels, Bio-Rad) and transferred onto a 0.45 µm PVDF membrane (Thermo Scientific™) ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blots were loaded at 2.5 to 50 ug total protein per lane in 4-20% gradient pre-cast polyacrylamide gels (#3450033, Bio-Rad). Gels were also loaded with the Dual pre-stained protein standards (#1610374 ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated by SDS-PAGE 4 - 12 % gradient Bis-tris gel (Novex) followed by protein transfer to a PVDF membrane (BioRad) and antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysates were fractionated by SDS-PAGE on a 10% gel or a 4-15% gradient gel (Bio-rad) and the separated proteins were transferred to a polyvinylidene difluoride membrane (Merck) ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... bound proteins were eluted with 2x Laemmli Sample Buffer and samples were then separated via SDS-PAGE using 4-20% Criterion TGX gels (BioRad) and transferred to a PVDF membrane ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 µg of protein was separated on a 4–20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Cancer Biology 2024Quote: Protein (30-60μg) were resolved via SDS polyacrylamide gel electrophoresis (SDS-PAGE) using precast 4-20% gradient polyacrylamide gels (Bio-Rad). Blocked membranes were probed with primary antibody for 3 hours at room temperature and subsequently for 1 hour species-appropriate secondary antibody at room temperature using enhanced chemiluminescence substrate (Pierce) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto either a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (4568094, Bio-Rad) or 4– 20% Mini-PROTEAN® TGX™ Precast Protein Gel (4561094 ...
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bioRxiv - Bioengineering 2024Quote: ... sonicated and heated before being processed via SDS-PAGE (,4-20% Mini-PROTEAN® TGX™ precast gels, 4561094, BioRad). Proteins were transferred to a membrane (Immobilon-FL PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... resolved on a self-cast 8% sodium dodecyl sulphate poly-acrylamide gel (SDS-PAGE) or on 7.5% or 4-20% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad), and transferred onto a Amersham Protran 0.45 nitrocellulose membrane (Cytiva) ...
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bioRxiv - Neuroscience 2024Quote: ... boiled for 5 min at 95 °C and then loaded on 4–15% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Gels were transferred onto polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were boiled for 10 minutes in loading dye containing 10% βME and run on 4-15% Protean SDS-PAGE gels (BioRad). Gels were stained with Coomassie Brilliant Blue R250 and imaged on a BioRad Chemidoc.
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bioRxiv - Neuroscience 2024Quote: ... 80-100 μg of protein were loaded onto 4–20% Mini-PROTEAN® TGX™ Precast Gel (Biorad, 4561093EDU, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... 30µg of RIPA lysate (starting material was included as a Western blot positive control) and samples were resolved on 4-15% Criterion Stain free gels (cat# 5678085 Biorad) at 200 Volts ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were centrifuged (12,000 xg) for 10 min at 4°C and the supernatant was mixed with 4X Laemmli sample buffer (Bio-Rad) containing DTT (12.5 µM final concentration) ...
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bioRxiv - Plant Biology 2024Quote: ... Thermo #26616) were run in pre-cast 4– 20% TGX stain-free denaturing protein gels (Biorad #4568093, #4568096, #5678094, #5678095) in a minigel system or criterion system (Biorad #1658005 ...
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bioRxiv - Biochemistry 2024Quote: ... Cell lysates (30 μg) were separated on precast 4-15% or 7.5% SDS-polyacrylamide Criterion gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... before heating at 95°C for 5 minutes and finally loaded onto 4-20% polyacrylamide gel (Bio-Rad, Cat # 4561094). Samples were then transferred to a PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured in Laemli Buffer and boiled for 10 minutes then run on a 4-20% Criterion TGX precast midi protein gel (BioRad) and transferred to a PVDF membrane (Millipore Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360, Bio-Rad) overnight at 4°C with rotation in DNA/RNA LoBind tubes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Immunology 2024Quote: ... 20μg sample per lane were loaded onto 4-20% precast polyacrylamide gel membrane and then transferred onto nitrocellulose membranes (BioRad). Membranes were labeled with anti-DDX41 (Novus Bio) ...
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bioRxiv - Physiology 2021Quote: ... and mixed 1:1 with 2x Laemmli buffer (Bio-Rad) plus 5% β-mercaptoethanol ...
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bioRxiv - Pathology 2019Quote: ... F4/80 (Clone Cl:A3-1, Bio-Rad, MCA497R, 1:100), CD3 (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... The blot was stained with 1:1 enhancer:substrate reagents (BioRad) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...