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Citations for Bio-Rad :
3201 - 3250 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Protein content was measured by Bradford assay and 30 mg of protein per sample were loaded into 4-20 % precast SDS-PAGE gels (Bio-Rad) under denaturing conditions ...
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bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
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bioRxiv - Biochemistry 2020Quote: ... After 24 h incubation at 4 °C the chelating agent was removed using a desalting column (Econo-Pac 10DG from Bio-Rad), pre-equilibrated with 20 mM Hepes buffer ...
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bioRxiv - Developmental Biology 2021Quote: Proteins extracted from Padi4CTL and Padi4CKO were subjected to SDS–PAGE (4–20% Mini-PROTEAN® TGX™ Precast gel, Biorad) and then transferred onto a nitrocellulose membrane ...
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Production and composition of group B streptococcal membrane vesicles varies across diverse lineagesbioRxiv - Microbiology 2021Quote: ... MVs (1.5 μg) were concentrated into a single band in a 4-20% Tris-Glycine SDS-PAGE gel (BioRad, Hercules, CA) that was fixed and stained using colloidal Coomassie blue (22).
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bioRxiv - Biochemistry 2021Quote: ... and the sample was heated to 90°C for 5 minutes before loading of 3 pmol onto a 4-15% Mini-PROTEAN TGX Stain-Free Precast Gel (Bio-Rad), alongside PageRuler Prestained Protein Ladder (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... equal amounts of protein for each sample was loaded on 4-15% Criterion TGX pre-cast midi protein gel (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein electrophoresis was performed by loading 20 μg of each sample onto 4–20% Bio-Rad Mini-Protean TGX Stain-Free Precast Gels (Bio-Rad). Gel imaging was obtained after activation using the Bio-Rad ChemiDoc Touch Imaging System ...
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bioRxiv - Neuroscience 2021Quote: ... cell lysates or cell media were subjected to SDS-PAGE using 4-20% gradient Tris-glycine gels (Bio-Rad; Hercules, CA) followed by transfer to Immobilon P membranes (EMD Millipore) ...
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bioRxiv - Pathology 2021Quote: ... and equal volumes of each suspension were loaded into two sodium dodecyl sulphate-polyacrylamide gels (SDS-PAGE; 4-20% Mini-PROTEAN TGX Protein Gels, Bio-Rad) and run at 150 V for one hour ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were electrophoresed on SDS-PAGE using 4-15% Mini-PROTEAN® TGX™ precast gel (Cat #456-1085, Bio-Rad), transferred onto Nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal masses of protein (5 ug – 40 ug) were separated by 4—15% of SDS/ PAGE and were transferred onto nitrocellulose membranes (Bio-Rad) for protein blot analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were loaded to each lane on a 4-20% gradient SDS/PAGE gel and transferred to nitrocellulose membranes (Bio-Rad). Signals were developed with HRP-conjugated secondary antibodies and SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
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bioRxiv - Genomics 2020Quote: ... heated at 95°C for 10 min and run on a Mini-protean TGX 4%-20% polyacrylamide gel (Biorad, 456-1095). The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were resolved using 4–20% precast polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA). After blocking ...
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bioRxiv - Microbiology 2020Quote: ... HEPES and TBT extractions and 20 μL from the TRIS and SDS samples were separated side-by-side on two separate pre-cast 4-20% TGX gradient gels (Bio-Rad) by SDS-PAGE and proteins transferred to nitrocellulose following the protocol described in (40) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times with 1X PBS for 5 min and developed with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate in alkaline phosphatase buffer (Bio-Rad).
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bioRxiv - Immunology 2021Quote: Lysates were centrifuged for 15 minutes at 4°C to recover supernatant and quantified for protein concentration using Protein Assay Dye Reagent Concentrate (Bio-Rad). 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were separated on a 4 to 12% on a 12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad) at 150-200V ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 µg protein was separated by electrophoresis on 4-12% polyacrylamide gels followed by transfer to nitrocellulose membranes (all gels/reagents from Bio-Rad). Membranes were then blocked (5% milk in TBS-Tween buffer ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates or lung homogenates in denaturing sample buffer (10-20 µg total protein) were separated by SDS-PAGE on 4-15% Mini Protean TGX gels (Bio-Rad) and transferred electrophoretically to a 0.45 µm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of the resulting fractions were run on a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad) at 200V for 30 min and stained by Imperial protein stain (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... boiled for 5 min at 95 °C and separated by discontinuous sodium dodecyl sulfate (SDS) 4-15% polyacrylamide gel electrophoresis (SDS-PAGE precast gels, Bio-Rad). The SDS-PAGE gels were stained in Coomassie Blue solution (Bio-Rad).
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bioRxiv - Immunology 2021Quote: Purified proteins were analysed for purity determination via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient gel (BioRad – 4568094), with or without the presence of 2-mercaptoethanol as reducing agent.
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bioRxiv - Immunology 2021Quote: ... In vitro translated proteins in 1x Lämmli buffer were proteins were separated on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and transferred to an Immobilon-FL PVDF membrane (Merck ...
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bioRxiv - Immunology 2021Quote: ... Cell lysate proteins were resolved on a 4–12% gradient SDS-PAGE gel and transferred to Trans-Blot nitrocellulose membrane (Bio-Rad). Blots were incubated with polyclonal goat anti-mouse TLR2 Ab (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... 30 µg of protein sample was loaded onto a 4 to 15% gradient sodium dodecyl sulfate (SDS)-polyacrylamid gel (Bio-Rad), followed by protein transfer to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: ... Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad). Membranes were incubated with the following primary antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... 2μg (pEGFP plasmid-based experiments) and 70μg (nodamuravirus-based experiments) were analyzed by SDS-PAGE on a 4-15% acrylamide gradient gel (BIO-RAD®) and gels were subsequently blotted onto a 0.45μm nitrocellulose membrane (BIO-RAD® ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates containing approximately 40 μg of protein were boiled for 10 min prior to separation by 4-20% SDS-PAGE (BIO-RAD). After transferring at 90V to a nitrocellulose membrane for 90 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of proteins were separated on either 10% SDS/PAGE gels or 4-20% Mini-PROTEAN TGX Stain-Free Gels (#4568094, Bio-rad). Proteins were then transferred onto PVDF membranes and probed with the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of each sample was determined as described before and a total amount of 20 μg protein/sample was resolved on 4-20% gradient gels (Bio-Rad). Proteins were transferred onto nitrocellulose or PVDF membranes futher blocked with 5% non-fat dry milk-TBST ...
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bioRxiv - Microbiology 2022Quote: ... Equivalent protein amounts (EVs and proteins) were separated on 4-15% stain-free pre-cast SDS-PAGE gradient gels (Bio-Rad) under non-reducing conditions and transferred onto PVDF membranes (Sigma Aldrich) ...
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bioRxiv - Genetics 2022Quote: ... Protein samples were heated at 37°C for 5 min and 10 μl was loaded on SDS–polyacrylamide gel electrophoresis (PAGE) gels (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad). After electrophoresis for 30 min at 200V ...
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bioRxiv - Genetics 2022Quote: ... total proteins were visualized by in-gel fluorescence using a trihalo compound incorporated in SDS–PAGE gels (stain-free TGX gels, 4–20%; Bio-Rad) after 45 sec UV-induced photoactivation using a ChemiDoc MP imager (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... 5 µL of the soluble fractions of cell lysates were run on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad) and stained using Gelcode Blue (Thermo Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... Digestion was confirmed by running 5 µL of digested or diluted samples on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad), followed by staining with Gelcode Blue (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 3-4 μg of total RNA was reverse transcribed using the iScript advanced cDNA synthesis kit (Bio-Rad). Quantitative PCR of target genes (Table 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 μL sample was run on an SDS-PAGE gel (Mini-PROTEAN TGX, 4-15%, Bio-Rad Laboratories, Inc., Hercules, CA) prior to transfer to a PDVF membrane (Immobilon-P Membrane ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 14000 rpm for 20 min at 4°C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). Fifty micrograms of total extract were then loaded in SDS-polyacrylamide gels and transferred onto nitrocellulose membranes for western blot analysis ...
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bioRxiv - Genetics 2022Quote: ... and 25µg of protein was loaded in each well and resolved on 4-15% gradient Tris-Glycine gel (Bio-Rad - 4561086). Semi-dry transfer was done onto 0.2µm Nitrocellulose membrane as per Trans-BlotTurbo Kit (Bio-Rad - 1704270 ...
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bioRxiv - Genetics 2022Quote: ... or 25 µg (mouse tissues) of proteins were loaded per lane on Any kD or 4-20% Criterion TGX stain-free precast gels (Bio-Rad) and transferred to Immobilon-FL PVDF membrane (Millipore ...
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bioRxiv - Genetics 2022Quote: ... 5% of the resulting lysate from the nuclear and cytoplasmic fractions were resolved by SDS-PAGE gel electrophoresis using 4-15% gradient gels (Bio-rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on 10% or 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were boiled for 5 min at 95°C before separation on a gradient 4 to 20% sodium dodecyl sulfate (SDS)-polyacrylamide gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semidry transfer (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... All radioactive protein samples were separated by size on a 4-to-20% Mini-Protean TGX precast SDS-PAGE gel (Bio-Rad). The gel was then dried and imaged using a Typhoon phosphorimager (Molecular Dynamics GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Synthetic Biology 2022Quote: ... Obtained soluble and insoluble fraction (pellet of cell debris) was analyzed by SDS-PAGE using 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and Precision Plus Protein Dual Color Standard (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
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bioRxiv - Neuroscience 2022Quote: Protein samples and dual plus molecular weight ladders were separated by SDS-PAGE and loaded on a Precast stain free gels with a 4–15% gradient (Bio-Rad). Proteins were transferred to PVDF membranes (Bio-Rad ...