Labshake search
Citations for Bio-Rad :
51 - 100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Droplets were generated using the QX200 Droplet Digital PCR System per the manufacturer’s protocols using Droplet Generation Oil for Probes (BioRad #1863005) and DG8 Cartridges and Gaskets (BioRad #1864007) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and DG8 Cartridges and Gaskets (BioRad #1864007). PCR was performed with the following cycle ...
-
bioRxiv - Cancer Biology 2024Quote: ... and ddPCR Supermix for Probes (No dUTP) (Biorad #1863024). Droplets were generated using the QX200 Droplet Digital PCR System per the manufacturer’s protocols using Droplet Generation Oil for Probes (BioRad #1863005 ...
-
bioRxiv - Cancer Biology 2024Quote: ... custom ddPCR primer/probes were designed as described above and ordered from BioRad. Then ...
-
bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed on 1 μg of RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). Then ...
-
bioRxiv - Cancer Biology 2024Quote: ... real-time qPCR was performed on a Bio-Rad CFX96 Touch Deep Well Real-Time PCR System using iTaq™ Universal SYBR® Green Supermix (Bio-Rad #1725125) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... Triplicate samples were run on a c1000 Thermal cycler (BIORAD). The temperature profile set for the run was 95 °C for 12 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... and transferred to a nitrocellulose membrane (BioRad, Hercules, CA). Membranes were blocked with 5% nonfat milk (LabScientific Inc. ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were quantified by the protein quantification assay (BioRad, Hercules, CA). Equal amounts of protein (40-50µg ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein lysates diluted in 4 X Laemmli Sample Buffer (Bio-Rad#161–0747) were loaded onto Bio-Rad 4–20% precast gels ...
-
bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred to a pre-activated PVDF membrane using the Trans-Blot®Turbo™ Transfer System and visualized using ECL (Bio-Rad Chemidoc). The following are the antibodies used in this study ...
-
bioRxiv - Cancer Biology 2024Quote: ... Western blot was done using BioRad pre-casted gels and a trans-turbo transfer machine (BioRad). Following electrophoresis ...
-
bioRxiv - Cancer Biology 2024Quote: ... and densitometric analyses were done using the QuantityOne software v4.6.7 (Bio-Rad). The membranes were stripped with stripping buffer (62.5 mM Tris ...
-
bioRxiv - Cancer Biology 2024Quote: ... Quantifications were performed with Image Lab software (Bio-Rad). Samples were normalized to the indicated loading controls ...
-
bioRxiv - Cancer Biology 2024Quote: ... Chemiluminescent visualization was performed with a ChemiDoc™ Imaging System (Bio-Rad) after incubation of the membranes with Clarity Western ECL reagent (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The assays were conducted using a calibrated Bio-Plex 200 system (Bio-Rad), and data analysis was performed with the Bio-Plex Manager software ...
-
bioRxiv - Cancer Biology 2024Quote: ... after incubation of the membranes with Clarity Western ECL reagent (Bio-Rad). Quantifications were performed with Image Lab software (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and captured by ChemiDoc MP Imaging System (Bio-Rad). For membrane re-hybridization ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA was retrotranscribed using the iScript™cDNA synthesis kit (Bio-Rad Laboratories AG ...
-
bioRxiv - Cancer Biology 2024Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentration of the supernatants were quantified by Bradford protein assay (Bio-Rad, 5000006). 30 µg of protein samples were used for immunoblotting with nitrocellulose membranes (Amersham Protran ...
-
bioRxiv - Cancer Biology 2024Quote: ... The signals were captured by ChemiDoc XRS+ Imaging System (Bio-Rad).
-
bioRxiv - Cancer Biology 2024Quote: ... First strand synthesis was performed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA) or qScript cDNA Synthesis Kit (Quantabio ...
-
bioRxiv - Cancer Biology 2024Quote: ... or iTaq Universal SYBR Green Supermix (Bio-Rad). Quantification of each gene was calculated using the ΔΔCt method with values normalized to housekeeping genes (Table 2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Signal was recorded using a ChemiDoc MP Imaging System (Biorad).
-
bioRxiv - Cancer Biology 2024Quote: ... and the microglia/macrophage marker F4/80 (BioRAD, MCA497) combined with the following secondary antibodies (FITC ...
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were blocked with 5% non-fat milk (Bio-Rad,1706404) in TBS containing 0.1% Tween20 (MilliporeSigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated with respective peroxidase-conjugated secondary antibodies and signals were detected using chemiluminescence with a ChemiDoc MP Imaging System (Biorad). Antibodies and dilutions are listed in Supplementary Table 2.
-
bioRxiv - Cancer Biology 2024Quote: ... The concentration of the protein samples was measured using Bradford protein assay (Bio-Rad, 5000006). 50 µg of whole cell lysate was used for SDS-PAGE and then transferred to PVDF membranes (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the DC Protein Assay Kit (Bio-Rad). 25-30 µg protein samples were run in SDS–PAGE followed by protein transfer using Mini Gel Tank and Mini Blot Module (Life Technologies) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The PDVF membranes were imaged by an imaging system (Bio-rad) according to the Enhanced Chemiluminescent (ECL ...
-
bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of up to 1μg of total RNA was performed using the iScript cDNA Synthesis Kit (Bio-Rad, #1725038).
-
bioRxiv - Cancer Biology 2024Quote: ... the total protein concentration of each sample’s supernatant was quantified with DC Protein Assay (cat no. 5000116, BioRad) in a 96-well plate ...
-
bioRxiv - Cancer Biology 2024Quote: ... The signal was then captured by ChemiDoc™ Touch Imaging System (Bio-Rad). Quantification of the relative protein expression in ΔPr-A9 cells compared to the WT cells was determined in Image Lab software (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Quantification of the relative protein expression in ΔPr-A9 cells compared to the WT cells was determined in Image Lab software (Bio-Rad). The average signal of duplicate spots subtracting background value from negative control spots represented the levels of each protein ...
-
bioRxiv - Cancer Biology 2024Quote: ... using a semi-dry method (Bio-Rad Trans-Blot® Turbo™ Transfer System). Membranes were blocked in 5% skimmed milk powder in tris-buffered saline-0.1% Tween 20 (TBS-T ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse transcribed to generate cDNA using the iScript Reverse Transcriptions SuperMix for RT-qPCR kit (Bio-Rad Laboratories). Real-time PCR was performed using the SYBR select Master Mix for CFX (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a PVDF membrane using the Bio-Rad Turbo Transblot System and transfer kit (Bio-Rad Laboratories) and fixed in methanol prior to blocking in 5% non-fat milk in TNT buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Cancer Biology 2024Quote: ... in hard-shell 96-well PCR plates (Bio-Rad Laboratories) on a CFX Connect Real Time System (Bio-Rad Laboratories) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were imaged on a ChemiDoc™ imaging system (Bio-Rad Laboratories). When necessary ...
-
bioRxiv - Cancer Biology 2024Quote: ... or Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards (Bio-Rad Laboratories) were included as protein ladders on the same gels ...
-
bioRxiv - Cancer Biology 2024Quote: SDS-PAGE Gels (8% or 10%) were transferred to nitrocellulose membranes (Bio-Rad) using a Trans-blot apparatus (2.5 A constant ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was quantified using the DC protein assay (Bio-Rad Laboratories) and a standard curve obtained with increasing concentrations of Bovine Serum Albumin (BSA ...
-
bioRxiv - Cancer Biology 2024Quote: ... on a CFX Connect Real Time System (Bio-Rad Laboratories). Primer sequences used were ...
-
bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using a CFX384 Touch Real-Time PCR Detection System (Bio-Rad). The thermocycling parameters were 50°C for 2 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was mixed with an equal volume of SDS-PAGE sample buffer (Bio-Rad) supplemented with 5% β-mercaptoethanol (Sigma) ...
-
bioRxiv - Cell Biology 2024Quote: ... After protein transfer onto methanol-activated PVDF membranes (Cat# 1620177, Bio-Rad), membranes were blocked using a 1% gelatin solution for 1 hour at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... and amplified using a CFX96 PCR Cycler (Bio-rad). Data was normalized by β-ACTIN levels ...
-
bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed in triplicate with the BioRad C1000 Touch™ Thermal Cycler and CFX384™ Real-Time System using SYBR Green PCR Master Mix (BioRad) together with sequence specific primers ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed using Superscript II RT and random hexamer primers according to the manufacturer’s instructions (BioRad). qRT-PCR was performed in triplicate with the BioRad C1000 Touch™ Thermal Cycler and CFX384™ Real-Time System using SYBR Green PCR Master Mix (BioRad ...