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Citations for Bio-Rad :
2801 - 2850 of 2973 citations for CBP p300 IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The relative amount of present HRP was detected by adding 60 µL/well DPBS + 1 mM CaCl2 and 20 µl/well HRP substrate (Bio-Rad, 170-5060). The plate was incubated for 10 minutes at room temperature before recording of luminescence on an EnVision plate reader (Perkin Elmer).
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bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and the protein were subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent of total protein loading and transfer ...
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bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and then subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent loading of total protein ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were synthesized from 1 μg total RNAs and qPCR were performed by using the iScript™ cDNA Synthesis Kit (BioRad, the Netherlands) and iQ™ SYBR Green Supermix (BioRad ...
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
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bioRxiv - Immunology 2021Quote: ... Transcript expression was measured by qRT-PCR with the appropriate primers (Supplemental Table 1) using a Bio-Rad MyiQ™ Real-Time PCR Detection System (Bio-Rad Laboratories) to measure SYBR Green (IQ™ SYBR® Green Supermix ...
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bioRxiv - Neuroscience 2021Quote: ... PAI-1 and insulin) from plasma samples were analyzed by duplicate using the Bio-PlexPro mouse Diabetes group from Bio-Rad (Ref. 171F7001M) by Luminex® 200TM technology as previously described (Ortega Moreno et al. ...
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bioRxiv - Neuroscience 2020Quote: ... cys free or AAA SNAREs were reconstituted at a 1:400 protein:lipid ratio of the same lipid compositions by dialysis in the presence of Bio-Beads (Bio-Rad Laboratories, Hercules, CA) into metal-free buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The following day it was probed with 1:2000 anti-Myc primary antibody (rabbit polyclonal, Santa-Cruz, A14) and with 1:10000 mouse anti-rabbit HRP linked secondary antibody (Bio-Rad, 170-6515). Amersham ECL prime HRP substrate (GE-Lifesciences ...
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bioRxiv - Plant Biology 2021Quote: ... The binary vector pMGE599 was introduced into Agrobacterium tumefaciens AGL-1 strain (62) through electroporation (E. coli Pulser; Bio-Rad, http://www.bio-rad.com). The scutella tissue of barley caryopsis was used for Agrobacterium-mediated transformation as described previously (63) ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription (RT) was performed with 1 μg of total RNA using SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, 172-5272). cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... After three washes (5, 10, 15 minutes) the blot was incubated for 60 minutes with secondary Goat Anti-Rabbit-HRP (Bio-Rad, 1:7000) and Alexa 647 Goat-Anti-Mouse antibodies (Molecular Probes ...
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bioRxiv - Cell Biology 2020Quote: ... transferred to 0.2 μm pore-size PVDF membranes, and blocked for 1 hour in blocking buffer (5% milk, 0.1% Tween-20 in 1x TBS (Bio-Rad, Hercules, CA, USA)) ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% skimmed milk in TBST for 30 min at room temperature then incubated with primary antibodies overnight at 4°C and secondary antibody conjugated with horseradish peroxidase (HRP) for 1 h at room temperature before visualization by ECL Plus Western blotting detection reagent (Bio-Rad, Hercules, CA). Semi-quantification of the bands were performed using NIH Image J software.
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bioRxiv - Plant Biology 2022Quote: ... CLso mature effectors (without signal peptide) were amplified from haplotype A and B infected tomatoes genomic DNA under standard PCR conditions using iProof (Biorad, Supplementary Table 1). Annealing temperatures of primer pairs were calculated using FastPCR (Kalendar et al. ...
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bioRxiv - Immunology 2022Quote: ... The affinity matrix was resuspended in 150 μl PB for the unbound and full plasma fraction and in 40 μl PB with a 1% blocking buffer background (Bio-Rad, The Netherlands) for the bound fractions ...
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bioRxiv - Microbiology 2022Quote: ... were used as secondary antibodies for 1 hour at the room temperature and revealed with the addition of Clarity Western ECL reagent (Bio-Rad Laboratories Ltd). ChemiDoc MP Imaging System (Bio-Rad Laboratories Ltd ...
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bioRxiv - Plant Biology 2021Quote: ... A second polyclonal antibody conjugated with alkaline phosphatase was used for immunodetection (1:1000) at wavelength 405 nm (BioRad Microplate Reader model 680) via the substrate p-nitrophenyl-phosphate ...
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bioRxiv - Neuroscience 2020Quote: ... plates were coated with 100 μl of either 1 μg/ml of rh-TfR1-ECD (purified in-house) or liver purified ferritin (Bio-Rad, 4420-4804) overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4° with primary antibodies diluted in blocking solution as follows: mouse α-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360); mouse α-SP56 (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... or POPC:palmitoyloleoylphosphatidylserine (POPS (85:15) at a 1:200 protein to lipid ratio by dialysis in the presence of Bio-Beads (Bio-Rad, Hercules, CA) into metal free buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... and α-TUBULIN (1:4000) were detected using SuperSignal™ West Pico or West Femto Chemiluminescent Substrate chemiluminescence in ChemiDoc MP imaging system (BioRad, Berkeley, USA). The densitometry analyses were performed using Bio-Lab Software ...
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Cell Biology 2019Quote: ... cells from a 10 cm dish were pelleted and lysed with 30 µl lysis buffer (20 nM Tris-HCl, 150 mM NaCl, 1% Triton X-100 (Bio-Rad, Hercules, CA), 20 mM beta-glycerol phosphate and protease inhibitors cocktail ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PPIases and PPIase fragments were vacuum aspirated onto a nitrocellulose membrane as serial dilution starting with 1 µg/ml using the dot blot system (Bio-Rad, Feldkirchen, Germany). Recombinant C3 toxin from C ...
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bioRxiv - Microbiology 2021Quote: ... The quality of extracted DNA samples was also checked by loading one μl of DNA in 1% Agarose Gel (Bio-Rad, California, USA). The extracts were stored at -20 C until use.
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bioRxiv - Developmental Biology 2020Quote: ... protein samples were boiled with β-mercaptol and ran in SDS-PAGE gels casted from 30% Acrylamide/Bis solution 29:1 (Bio-rad, 1610156) using the Mini-PROTEAN® system (Bio-rad) ...
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bioRxiv - Genomics 2019Quote: ... the integrity and quantity of total RNA was confirmed by 1% agarose gel electrophoresis and the Experion™ automated electrophoresis system (Biorad Laboratories, Australia).
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bioRxiv - Physiology 2021Quote: ... Consistent protein loading and sufficient transfer of proteins was confirmed using 1× Amido Black stain and images were captured using a Versadoc System (Bio-Rad, Mississauga, ON). Membranes were blocked overnight at 4°C with Tris-buffered saline (TBS)/0.1% Tween 20 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and gene expression of MCL-1 in the parental and MCL-1 deleted cells were verified by qPCR performed using the iTaq™ Universal SYBR Green Supermix (BioRad, California, USA), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The total RNA (1 μg) from each sample was reverse transcribed into cDNA using the iScript cDNA select Synthesis Kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNAs were subjected to PCR analyses using specific primers (The primer sequences are listed in Table 1) on an amplification detection system (Bio-Rad, Hercules, USA). β-actin served as a housekeeping gene ...
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bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was diluted 1:40 in water before using 1 μL as template in 10 μL qPCRs with iTaq™ Universal SYBR Green Supermix (Catalog# 1725121, Bio-Rad, USA) and 400 nM each primer ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and OD600 were measured from bacterial cultures diluted 1:10 using a Synergy H1 plate reader (Bio-Tek, Winooski, WI) and a Bio-Rad spectrophotometer (Bio-Rad, Hercules, CA), respectively ...
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bioRxiv - Immunology 2022Quote: ... and 1 µl of respective primer pairs (200 nM final concentration) were mixed and distributed into 96-well qPCR plate (Bio-Rad; cat. #12001925). Droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were boiled 1 min and subjected to SDS-PAGE using a 4-15% Mini-PROTEAN TGX Stain-Free Protein Gel (Bio-Rad Ref. 4568085). After SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: 100 microlitres of the secreted fractions and 100 µl of 1:20 diluted (in PBS) lysates from the secretion assays were loaded into a bio-blot microfiltration blotting system (BioRad. Cat. Nº: 1706545). Samples were left to flow through a 0.45 µm nitrocellulose blotting membrane (Amersham Protran ...
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bioRxiv - Cell Biology 2022Quote: ... Then membranes were washed 3 times with TBS-T and incubated with goat anti-mouse or anti-rabbit IgG HRP-conjugated secondary antibody (1:10000 in 5% BSA TBS-T, Bio-rad; #1706515, #1706516) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer: 2% Blotting Grade Blocker (Bio-Rad cat. # 1706404) and 2% heat-inactivated goat serum (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. no. 1708890) or Superscript III (Invitrogen cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times in TBST prior to incubation with secondary antibody (1/5000 dilution horseradish peroxidase (HRP)-conjugated anti-mouse (Bio-Rad, 170-6516) or anti-rabbit (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA). qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The results were obtained from channels 1 (FAM) and 2 (HEX/VIC) and analysed with QuantaSoft™ Analysis Pro 1.0 (Bio-Rad Laboratories, Inc). All ddPCR reactions were prepared with QX200™ ddPCR™ EvaGreen® Supermix (2x).
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bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Immunology 2023Quote: ... The expression of immune system markers were mostly studied with inmunohistochemistry using the following antibodies: mouse anti rat CD68 (dilution 1:300 BIORAD, ref. MCA 341R) for monocytes/macrophages ...
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bioRxiv - Microbiology 2023Quote: The resulting amplicons and their corresponding lengths were visualized and documented via gel electrophoresis and analysis (Quantity 1 software, Bio-rad, CA, USA).
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...