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Citations for Bio-Rad :
2701 - 2750 of 2973 citations for CBP p300 IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Expression levels of ICAM-1 and IL6 mRNAs were quantified using SYBR Green-based primers and the iTaq master mix (Bio-Rad Inc) in the Bio-Rad CFX Real-Time PCR detection system (Bio-Rad Inc) ...
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bioRxiv - Immunology 2022Quote: The collected fractions were then pooled by the expected oligomeric state for both human milk and serum and again IgAs were captured on beads with a 1% blocking buffer background (Bio-Rad, Netherlands) and addition of the 7D8-IgA1 and 5D5v2-IgA1 at 0.2 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... and cDNA was reverse transcribed from 1 ug of the resultant RNA with the Bio-Rad iScript kit (Bio-Rad 170-8891) in a reaction volume of 20 µl ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3-HB and n-butanol were separated with 5 mM sulfuric acid as the mobile phase and one of two column conditions: (1) an Aminex HPX-87H or Fast Acids anion exchange columns (Bio-Rad Laboratories) at 35 or 55 °C and a flow rate of 0.6 ml min-1 or (2 ...
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bioRxiv - Microbiology 2020Quote: ... containing 2 g l-1 glucose and 1.32 mM K2HPO4 by treating prepared media with a universal chelator (Chelex® 100 Chelating Resin, Bio-Rad 1421253), filtering sterilizing away the Chelex resin ...
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bioRxiv - Microbiology 2021Quote: ... the gel was stained with 1 mg/mL ethidium bromide and visualized with the ChemiDoc XRS system (Bio-Rad Laboratories, CA, USA). Genetic similarities were measured using the Dice coefficient and the clustering was carried out by the unweighted-pair group method with arithmetic averages (UPGMA ...
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bioRxiv - Developmental Biology 2021Quote: ... Following 4x 10 min washes with PBS-T the membrane was incubated for 1 min with Clarity Western ECL Substrate (170-5060, Bio-Rad Laboratories) and imaged with Amersham™ Imager 600 (GE ...
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bioRxiv - Microbiology 2019Quote: ... and visualized with rabbit-α-FLAG (1:3,000) primary and goat-α-rabbit-HRP conjugated secondary antibodies on the ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: Two hundred µL of overnight bacterial cultures were transferred 5 mL of a 1:10 dilution of TSB medium (Bio-Rad, USA) supplemented with 1 mg mL-1 L-tryptophan and incubated at 30°C overnight (Gordon & Weber 1951) ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using 1:10 dilutions of cDNA synthesis products with the iTaq Universal SYBR green supermix (Bio-Rad; 1725121) on a CFX Connect real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The separated protein was transferred to a nitrocellulose membrane in Towbin buffer 1× using the Mini Trans-Blot system (Biorad, United States) at 120mA for 120 minutes in ice cold conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... reverse transcription (RT) was carried out with 1 µg of total RNA using the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Cell Biology 2021Quote: ... 12.5 ng of cDNA of each sample were pre-amplified using 1 μL of all primers mix (500 nM) in a T100 Thermal cycler (BioRad, Hercules, CA), using the following program ...
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and analyzed for the expression of the exosome production-related genes listed in Appendix Table 1 by RT-PCR on a CFX Connect Real-Time System (Bio-Rad Laboratories).
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bioRxiv - Neuroscience 2021Quote: ... was incubated at 65°C for 10 min and at 85°C for 1 min into a T100™ thermocycler (Bio-Rad). The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™) ...
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bioRxiv - Physiology 2021Quote: ... were used for real-time PCR with gene-specific primers (Supplemental Table 1) and Tbp as a house keeping gene on a CFX96™ Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Genomics 2021Quote: ... Reverse transcription (RT) was performed on 1 μg of DNase-treated total RNA using the iScript cDNA synthesis kit (Bio-Rad #1708890), with the same amount of total RNA diluted in water as negative controls for RT-PCR ...
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bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... and purified from remaining salts using Dowex 50 W X 8 50-100 mesh (Bio-Rad, 10 x 1 cm, H+ form) equilibrated with water ...
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bioRxiv - Immunology 2021Quote: ... the enzyme reaction was stopped with 50 μL of 1 M sulfuric acid per well and the absorbance was measured in Bio-Rad Model 550 microplate reader (Bio-Rad Laboratories). Sera were assayed in duplicates and antibody titer represents the last reciprocal serum dilution above blank.
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bioRxiv - Immunology 2020Quote: ... Conjugate DNA was measured by qPCR amplification from 1 μL of lysate in a 10 μL reaction containing 5 μL of iTaq Universal SYBR Green Supermix (Bio-Rad, 1725125) and 5 pmol of each primer (Supplementary Table 1) ...
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bioRxiv - Immunology 2019Quote: ... Upfront tagmentation of ~35,000 nuclei was performed for 1 h at 37°C in a C1000 Touch thermal cycler with 96-Deep Well Reaction Module (Bio-Rad, 1851197). Tagmented nuclei were mixed the master mix and loaded onto a Chromium Chip with Gel beads and partitioning oil on different wells ...
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bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were visualized with goat anti-mouse or goat anti-rabbit IgG secondary antibodies (1:5000) diluted in 2% Omniblot milk (AmericanBio) in 1X TBST using a chemiluminescence detection system (BioRad ChemiDoc MP).
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bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were blocked with Bovine Serum Albumin (BSA) in DPBS for 30 min and then incubated with anti-His6 antibody (1:100, Bio-Rad, MCA1396) for 2 h at 25°C ...
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bioRxiv - Cell Biology 2022Quote: ... for 20 minutes treated with PBS containing 1 % w/v BSA and 0.1 % v/v Triton X-100 (Bio-Rad, München, Germany) for 30 minutes ...
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bioRxiv - Synthetic Biology 2022Quote: ... #FABRK 001-1) and 400 ng of cDNA was synthesized using the iScript™ Select cDNA Synthesis Kit (Bio-Rad Laboratories, #1708897) on a Mastercycler® nexus PCR Thermal Cycler (Eppendorf) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantified by nanodrop and cDNA was produced from 1□μg of purified RNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories). qPCR reactions were performed using the SsoFast Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were run on a 1% Agarose gel containing .08% ethidium bromide and visualized using the ChemiDoc imaging system (BioRad, Hercules, USA). PCR amplicons were sanger sequenced (Psomagen ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Neuroscience 2022Quote: ... The diluted cDNA (1:10) was used as a template for real-time PCR (Bio-Rad CFX 96™ Real-Time system) with an SYBR® Green Supermix mixture following the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Germany) and 1 μg of total mRNA was translated to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the instructions.
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was diluted to 1:200 (snRNA KD) or 1:25 (motor neurons and EXOSC10 KD) and qRT-PCR was performed with Ssofast EvaGreen (Bio-Rad, 1725201) with the following cycling conditions ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0, 150 mM NaCl, 1% NP-40 [United States Biological, 19628], 0.5% deoxycholate [Sigma-Aldrich, D6750], 0.1% SDS [Bio-Rad, 1610302]) containing protease inhibitor cocktail and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates were boiled in loading buffer [1:9 ratio of 2-mercaptoethanol:4x Laemmli Sample Buffer (Cat.#1610747; Bio-Rad Laboratories)] for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Molecular Biology 2024Quote: ... 10% Acrylamide:Bis Acrylamide) for 1 hour and transferred to a nitrocellulose membrane (0.45µm) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... at 75 V for 15 min and 100 V for 1 hr 15 min using 1X Tris/Glycine/SDS running buffer (BioRad Cat. # 1610772). Following electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Developmental Biology 2023Quote: ... Transfer buffer was prepared as a 1X final solution by mixing 7:2:1 of ddH2O: methanol: 10X Tris/Glycine Transfer Buffer (Bio-Rad #1610734). Following transfer ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were detected using chemiluminescence using horseradish peroxidase–conjugated secondary antibodies of anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) origin and enhanced chemiluminescence substrate (Thermo Scientific # 32106) ...
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bioRxiv - Immunology 2024Quote: ... A 3.3 mm DBS punch from each sample was combined with 250 µl elution buffer (Tris-buffered saline, 1% casein blocker) (Bio-Rad Laboratories) in a 96 well plate ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, USA). The detection limit was calculated to be 1000 copies per reaction ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...