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Citations for Bio-Rad :
2751 - 2800 of 2973 citations for CBP p300 IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 15,000 x g for 1 min in a tabletop centrifuge to pellet cell debris and the supernatant was diluted 1:10 in sterile ddH2O for a 96-well microplate Bradford microassay (Bio-Rad 5000201) to estimate the amount of leaked protein ...
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bioRxiv - Bioengineering 2023Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... wre incubated for 1 h at RT and membranes were revealed by using ECL reagents with the ChemiDoc imager (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2023Quote: ... cDNA was diluted 1:10 in nuclease free water prior to qPCR analysis using a CFX384 real-time PCR system (Bio-Rad, USA). Reaction mixtures containing 5 μl PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) synthesis was performed using 1 μg of total RNA with the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The genes encoding for the zonula occludens-1 (Zo-1) ...
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bioRxiv - Neuroscience 2023Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS solution with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
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bioRxiv - Physiology 2023Quote: ... The blots were washed four times with 0.1% tween TBS before being incubated for 1 h at room temperature with goat Anti-rabbit IgG HRP conjugated secondary antibody (Bio-Rad, USA) in 0.1% tween TBS buffer containing 3% BSA (Bovogen ...
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bioRxiv - Plant Biology 2023Quote: ... The 1st strand cDNA was synthesised from 1 µg of RNA for each sample using iScript™ cDNA Synthesis Kit (Bio-Rad). This cDNA was then used as the template in qRT-PCR reaction mixture after 10 times dilution ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... qPCR reactions were conducted using 1 μl of cDNA product in 10 μl total reaction volume using Itaq Universal SYBR green (Bio-Rad, #1725121) with primers at a concentration of 500nM ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody incubation was done at room temperature for 1h (HRP-conjugated goat anti-rabbit IgG, 1:2000 dilution, Bio-Rad, 403005). Membranes were washed with Tris-buffered saline containing 0.05% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... before injecting into 1 mL Hitrap Heparin HP column pre-equilibrated with Wash Buffer C operated in the BioLogic DuoFlow™ FPLC system (Bio-rad). The protein was eluted from the column by running a gradient flow rate that exchanges Wash Buffer C and Elution Buffer D (2000 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of inflammatory cytokines were quantified using Bio-Plex Pro Human Inflammation Panel 1 37-plex (#171AL001M) magnetic bead-based assay (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Gel demonstrating protein purity was generated using by loading 1 μg of each protein onto a Mini-PROTEAN TGX 4-20% gradient gel (BIO-RAD, 4561096) and then staining with Coomassie blue.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were detected using chemiluminescence using HRP conjugated secondary antibodies of either anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) or anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated for 1-2 h at 4 °C and incubated overnight with pre-washed bio-beads (Bio-Rad Laboratories) at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized with 1 µg of total RNA by using the iScript cDNA Synthesis Kit (Bio-Rad, Cat# 1708891). cDNA was diluted 20 times and real-time quantitative PCR was performed by using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction collection was performed using a Masterflex L/S easy-load II (Cole Parmer, 77200-50) running at approx 1 mL/min and BioFrac™ fraction collector (Bio-Rad) with a fraction time window of 2 min ...
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bioRxiv - Immunology 2023Quote: ... and cell pellets were lysed in 1× Laemmli buffer before proteins were separated on 4-20% Mini- PROTEAN® TGXTM Precast Protein Gels (Bio-Rad) and transferred to a nitrocellulose membrane (Odyssey® nitrocellulose membrane ...
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bioRxiv - Microbiology 2023Quote: ... before destaining with Tris buffered saline Tween-20 (TBST) and incubation for 1 hour with blocking buffer (TBST with 50 g/L blocking agent (Bio-Rad, 1706404)) ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated for 1 h in water with 3X GelRed (Phenix Research Products) and imaged on ChemiDoc XRS imaging system (Bio-Rad Laboratories) and Quantity One imaging software (Bio-Rad Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... a total of 20 µL of 1 µg/µL plasmid DNA was combined with 50 µL of gold microparticles (1 µm, 50 mg/ml, suspended in 50% (v/v) sterile glycerol) (#1652263, Bio-Rad, USA), 100 µL 2.5 M CaCl2 and 40 µL 0.1 M spermidine (S0266 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane using the iBlot 2 device and membranes were blocked for 1 hour at RT in 5% (w/v) Blotting Grade Blocker/PBS (Biorad, #170-6404). GCase was detected using rb mAb to hGBA antibody (abcam ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0, 150 mM NaCl, 1% NP-40 [United States Biological, 19628], 0.5% deoxycholate [Sigma-Aldrich, D6750], 0.1% SDS [Bio-Rad, 1610302]) containing protease inhibitor cocktail and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Immunology 2024Quote: RNA was isolated (ZymoResearch R2050) from the infected Hsu cells at various passages and 1 μg of RNA was used to prepare cDNA (BioRad 170-8891). The cDNA was used as the template for various PCR reactions targeting the 4 genes of interest - E ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.5 μL of forward and reverse primers (see Supplementary Table 1 for primer sequences) for the genes of interest were combined in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a CFX384 Real Time PCR Detection System using the following program ...
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bioRxiv - Developmental Biology 2024Quote: ... see Supplementary Table 1 for primer sequences) and diluted to 20 μL with nuclease free water in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a Bio-Rad CFX384 Real Time PCR Detection System with the following program ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then rinsed 3 times with PBS to ensure the complete removal of glutaraldehyde and blocked for 1 h in blocking buffer (5% milk (Bio-Rad #1706404) in Tris-Buffered Saline (TBS ...
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bioRxiv - Plant Biology 2024Quote: ... and 1 µl of cDNA was used as a template for qRT-PCR using iQTM SYBR® Green Supermix (BIO-RAD, USA) on a CFX96TM Real-Time PCR machine (BIO-RAD ...
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bioRxiv - Immunology 2024Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The stain-free gel was UV-activated for 1 minute and imaged for total protein (BioRad Chemi Doc XR+, Hercules, CA, USA) with ImageLab software (BioRad Image Lab v6) ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μL aliquots were mixed with 1 μg of the plasmid and transferred to 0.2 cm electro cuvettes (BIO-RAD, Hercules, USA), chilled for 15 min on ice ...
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bioRxiv - Biophysics 2024Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... concentrated fractions containing CDT holotoxin were loaded onto a gravity flow size exclusion column (1 g, P-60 resin; #1504164, Bio-Rad, USA) pre-equilibrated with PBS pH 7.4 as the mobile phase and 500 μL elution fractions taken.
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were prepared at a concentration of 1 μg/μL and 15 μL of sample was loaded onto 4-15% gradient SDS-polyacrylamide gels (Bio-Rad Laboratories). Following the electrotransfer of proteins onto PVDF membranes ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
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bioRxiv - Immunology 2024Quote: ... were covalently coupled to carboxyl-functionalized fluorescent magnetic beads (1-3µg/1.25*10^6 beads) (Luminex Corp. MagPlex® Microspheres; Bio-Rad; Temse, Belgium) employing the BioPlex amine coupling kit (Ref. ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with fresh serum-free medium containing 10% CCK-8 for 1 hour at 37°C and then the absorbances at 450 nm were measured using a microplate reader (Bio-Rad, Hercules, USA).
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg DNA from each sample was transferred to a nitrocellulose membrane using the Bio-Dot SF microfiltration apparatus (Bio-Rad, #170-6542). The membrane was blocked in 5% non-fat milk in TBS with 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.08 of each M13 dye and 1 μL of the DNA template using a Bio-Rad thermocycler T100 (Bio-Rad, Pleasanton, CA, USA). PCR products were analyzed on an ABI 3500 genetic analyzer and sized against the LIZ500 internal standard (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... an affinity purified antibody generated against the SAX-7 cytoplasmic tail [gift of (Chen et al., 2001)] and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). For the loading control ...
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bioRxiv - Microbiology 2022Quote: ... and collecting 1 mL fractions from the top at a pace of 1 mL per minute while continuously measuring A254 using the BioLogic LP system (BioRad, Hercules, California, USA).
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bioRxiv - Genomics 2019Quote: ... Total HIV-1 DNA content of CD4 T-cell DNA isolates was measured by digital droplet PCR (ddPCR, QX200 platform, Bio-Rad, Temse, Belgium), as described by Rutsaert et al.56 The DNA was subjected to a restriction digest with EcoRI (Promega ...
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bioRxiv - Immunology 2019Quote: Mouse patellofemoral joints and hearts were homogenized using an electric homogenizer with toothed blades in tissue lysis buffer (Machery-Nagel, 740962) containing beta-mercaptoethanol (1:100) (Bio-Rad, 161-0710) and Triton-X-100 (1:100 ...
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bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...