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Citations for Bio-Rad :
2651 - 2700 of 2973 citations for CBP p300 IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were applied for 1 hour at room temperature (1:5,000; CST, #7074) before detecting protein bands at the BioRad ChemiDoc MP Imaging System using chemiluminescence (BioRad, 170-5061).
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bioRxiv - Neuroscience 2024Quote: ... The gel was electrophoresed for 10 minutes at 50V then 1 hour at 120V in Tris-Glycine SDS buffer (Bio-rad). The gel and nitrocellulose membrane were then equilibrised in transfer buffer (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The cell mixture was transferred to a 1 mm electroporation cuvette and pulsed using a Gene Pulser Xcell Electroporation System (Bio-Rad) using 2.1 kV/cm ...
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bioRxiv - Immunology 2024Quote: ... After final wash and supernatant removal, 1x Loading Buffer (40 μL, 3:1 Lysis Buffer:4x Laemmli Loading Buffer (Bio-Rad)) was added to the sample and the sample boiled (10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 ng template library DNA[21] in a total reaction volume of 20 µL using T100 Thermal Cycler (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times with and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Each well was then blocked with 150 µL of blocking buffer consisting of 1×PBS + 4% (w/v) nonfat milk (Bio-Rad). The plates were blocked for 1 h at room temperature and then washed five times with PBST ...
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bioRxiv - Cell Biology 2024Quote: ... After gel polymerization was performed pre-electrophoresis of empty gel in 5% acetic acid (running buffer) for 1-1.5h/150 V in reverse polarity of electrophoretic BioRad system (Bio-rad, USA). The running buffer was discarded ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated on 10 % polyacrylamide gels and blotted onto a nitrocellulose membrane at 100 V for 1 h using the Mini Trans-Blot system (Bio-Rad). Membranes were blocked with 5 % milk powder in PTw (1x PBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of total RNA from each sample was converted to cDNA using iScript™ Reverse Transcription Supermix (Bio-Rad, 1708841). Then qPCR reactions were set up using TaqMan® Fast Advanced Master Mix (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... was added to all samples and the products were separated on 15% polyacrylamide denaturing urea gels (19:1 acrylamide-bisacrylamide, Bio-Rad). The gels were fixed in fixing solution (40% methanol ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was used as template for real-time PCR (primers in Supplemental table 1) using CFV384 Touch™ Real-Time PCR Detection System (Bio-Rad). Housekeeping genes (AT4G34270 ...
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 1 μg of RNA was subjected to retrotranscription using iScript™cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... slices were bombarded with biolistic particles created by coating 1.6 μm gold particles (Bio-Rad, #165-2262; ∼1 μg DNA/mg gold) with constructs in mammalian expression plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. #: 1708890). cDNA was diluted to 20 ng/µl and 2.5 µl/reaction was used as input for the qPCR reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad, catalog 161-0737) containing 10% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2019Quote: ... Gene silencing efficiencies were performed as described previously18 with the following modifications: cDNA was synthesized from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, #1708891) following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: ... Gene expression was assessed using commercially available Taqman primer/probe sets (Thermo Fischer) or specific oligonucleotide pairs (Table 1) and SYBR® Green supermix (Bio-Rad). Relative expression of the target genes was calculated with the comparative Ct method [43] using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent analysis was conducted on a 12% SDS-PAGE gel and blots were probed with either mouse α-V5 (SV5-Pk1, 1:1000, Bio-Rad), mouse α-HA (HA.11 ...
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bioRxiv - Immunology 2021Quote: ... in 0.1% BSA in 0.1% TBST and subsequently imaged using HRP-conjugated anti-rabbit antibodies (Bio-Rad Cat#172-1019, 1:10000) and Western Lightning Plus-ECL (PerkinElmer Cat#El103001EA ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with ethidium bromide (0.5 mg mL-1) and photographed using the Quantity One electrophoresis analysis system (Bio-Rad, CA, USA). Gel images were digitally normalized to a single DNA marker to reduce gel-to-gel banding pattern variability ...
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bioRxiv - Neuroscience 2020Quote: ... Up to 1 μg of RNA was used to synthesise cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) and PowerUp SYBR Green (Thermofisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Gel electrophoresis was performed on a 1% agarose gel and imaged using Molecular Imager® Gel Doc™ XR (Bio-Rad, USA).
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of RNA was used to generate cDNA with the iSCRIPT Clear kit (Bio-Rad, Hercules, CA, cat# 170-8891). For the qRT-PCR ...
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bioRxiv - Systems Biology 2021Quote: Samples were normalized to 1 mg/mL protein in 0.1% Azo by the Bradford assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006) using bovine serum albumin to generate a standard curve ...
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bioRxiv - Plant Biology 2020Quote: ... or 1 μl (for the rest) of a 1/2 dilution of the cDNA was added to a reaction containing 1X of SsoFast EvaGreen (Bio-Rad, USA), 0.5 μM Forward and Reverse primers (Table 2) ...
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bioRxiv - Plant Biology 2020Quote: ... we used 2 µl of a 1/5 dilution of the cDNA obtained as above in a reaction containing 5 µl of SsoFast EvaGreen (Bio-Rad, USA), 0.5 µl of Forward primer (10 µM ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed three times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1,706,515, 1:10,000) secondary antibodies for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were made at a 9:1 ratio of sgRNA:Cas9 (90pmol:10pmol) in Gene Pulser® Electroporation Buffer (Bio-Rad, 1652677) and incubated for 10 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All qPCR amplifications were performed in duplicate and each contained 15 µL total volume of 1 × SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA), 0.3 µM of each primer ...
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bioRxiv - Cancer Biology 2021Quote: ... 60 of High Capacity NeutrAvidin slurry (Thermo PI29204) were washed three times with 1 mL 2X RIPA/1mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231) ...
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bioRxiv - Microbiology 2022Quote: ... The melt curve analysis was performed at 65°C for 1 minute in CFX connects TM Real-time PCR detection system (Bio-Rad, USA). The primers used are listed inTable 1 ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 4 ul of diluted cDNA (1:40 dilution rate) were used for each reaction with 5 ul SsoAdvanced Universal SYBR® Green Supermix (BIO-RAD) and 1 ul of primer mix (5uM) ...
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bioRxiv - Neuroscience 2022Quote: ... 1:4 & 1:8) and loaded in triplicates onto a nitrocellulose membrane assembled in a 96-well dot-blot apparatus (Biorad, Cat#1703938). The membrane was then stained with Ponceau (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
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bioRxiv - Biophysics 2022Quote: ... TMEM87A in buffer 1 was exchanged into soybean l-α-phosphatidylcholine (Soy PC, MillaporeSigma) with the addition of Biobeads SM2 (Bio-Rad) and an hour incubation at a protein:lipid ratio of 1:10 (corresponding to 0.4 mg purified TMEM87A and 4 mg of Soy PC lipid or 1:50 in buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... Electrophoresis was carried out for 1 hour at 90 V using an electrophoresis system (mini–DNA SUB CELL, Bio-Rad, Mississauga, Canada). The DNA fragments were visualized in a Gel Doc XR+ Gel Documentation System (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad) and stored at −20 °C overnight ...
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: cDNA was synthesized using 1 μl of total RNA from melanocytes or iridophores with iScript™ Advanced cDNA Synthesis Kit (#1725038, Bio-Rad) following by 6 cycles of pre-amplification with pooled 47 pairs of MTE primers designed for NanoString CodeSet (SsoAdvanced™ PreAmp Supermix ...
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bioRxiv - Immunology 2020Quote: ... 40 cycles of 15 s 95°C and 1 min 60°C) was carried out using a CFX 96 (BioRad, Hercules, USA) qPCR machine ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA concentration was estimated by nanodrop and 1 μg of RNA was used to synthesize cDNA with iScript cDNA synthesis kit™.(Biorad 1708890) qPCR was performed using Precision™ 2X qPCR master mix (Primerdesign ...
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bioRxiv - Neuroscience 2022Quote: ... macrophages were plated in a 24-well plate containing cover slips and incubated with the Magic Red cathepsin B substrate (1/250) (Bio-Rad, ICT938) for 2h ...
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bioRxiv - Neuroscience 2022Quote: ... then placed in 4% normal goat serum in 0.01M PBS blocking solution for 60 minutes with agitation followed by incubation with rat anti-CD68 (1:500; BioRad, cat no. MCA1957GA) and rabbit anti-MBP (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 μg of RNA was reverse transcribed into cDNA using BUNiScriptTM cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 170-8891). For pancreas samples ...