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Citations for Bio-Rad :
2651 - 2700 of 2723 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The cell suspension then was divided equally and heated for 3 min at indicated temperatures on T100™ Thermal Cycler (Bio-Rad Laboratories). Cells were snap-frozen and thawed twice and centrifuged at 17.000 g for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit). Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µL of the first PCR amplification product was then used for analysis on a 2% ethidium bromide-stained agarose gel (120 minutes at 80V in a Mini-Sub® Cell GT gel chamber connected to a PowerPac™ HC (Bio-Rad) power supply ...
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bioRxiv - Biochemistry 2022Quote: ... The cell suspension was transferred to a 0.2 cm gap cuvette and immediately electroporated by GENE PULSER II (Bio-Rad, Hercules, CA, USA) at 0.45 kV and 50 μF ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were collected and stored at −80□°C and cells were lysed in 1x Laemmli Buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) for further analysis.
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bioRxiv - Bioengineering 2023Quote: ... at 100V with a constant current for 60 mins at 4°C in a Bio-Rad Mini-Transfer Cell (Bio-Rad, Cat# 1703930) according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2023Quote: ... or both at the University of Georgia (Athens, Georgia) Flow Cytometry Core Center using a Bio-Rad S3e cell sorter (Bio-Rad, Irvine, California).
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bioRxiv - Microbiology 2024Quote: ... The recombinant protein was transferred to 0.45 μm nitrocellulose membranes using the Trans-Blot® Semi-Dry Transfer Cell (Bio-Rad, CA, USA), and bands were confirmed by 0.1% Amido black staining solution (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Percentage of targeted alleles within a cell population was measured with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7; Bio-Rad, Hercules, CA, USA) using the following reaction mixture ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System; Bio-Rad; 250 V, 500 μF, 0.4-cm cuvettes). Cells were subsequently plated on MEF feeder layers (DR4 MEFs for puromycin or neomycin selection ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were separated in 12.5 % sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE; 100V for 90 minutes) in Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad, Hercules, CA, USA) and transferred into polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2019Quote: ... Second-dimension sodium dodecyl sulphate-polyacrilamide gel electrophoresis (SDS-PAGE) was done on 12.5% Tris-HCL Precast Gels using a Criterion electrophoresis cell (Bio-Rad Laboratories, Hercules CA, USA), run at 80V for 20 min before switching to 100V for approximately 2 h ...
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bioRxiv - Bioengineering 2019Quote: ... Equal volumes of the cell suspension (RT) and the agarose solution (75°C) were mixed and immediately transferred to plug molds (Bio-Rad Laboratories, Cat. # 1703713), ∼100 μl mixture per plug ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μg proteins of the cell line were exposed to a 10% SDS-PAGE gel and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... the cell lysates were separated by 15% SDS-polyacrylamide gel electrophoresis and electro-blotted onto a Immun-Blot PVDF Membrane using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 2% skimmed milk and probed with antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... or self-made gel and subjected to a sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) using a Mini-PROTEAN® Electrophoresis cell (Biorad, Cat No 1658002EDU). Gels were run at 130 V for approx ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of transcribed RNA were electroporated into 8×106 Vero cells using the Gene Pulser Xcell™ Electroporation Systems (Bio-rad, California, USA) under as described previously41 ...
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bioRxiv - Cancer Biology 2021Quote: Separation of 20 μg or 50 μg protein from cell lysate was performed on a 10% Mini-PROTEAN® TGX™ gel (BioRad, Portland, ME) and subsequently transferred onto Immunoblot PVDF 0.45μm membranes (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... Equal concentrations of samples (15μg for whole cell lysates and 5μg for nuclear fractions) were resolved on Criterion gels (Bio-Rad, catalog number 5671093 and 5671094) and transferred to nitrocellulose (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... protein extracts were obtained from mock-or MAYV-infected HeLa cells or HDFs that were treated or untreated with PIM kinase inhibitors in Laemmli buffer with 10% Dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were separated in SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were solubilized in Laemmli sample buffer and heated at 95°C for 10 min to denature proteins and separated via SDS-PAGE for 40–60 min at 270 V using 4–20% stain-free gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Thereafter ...
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bioRxiv - Cancer Biology 2022Quote: Equal amounts of cell lysate were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Blocking was performed for 60 min with 5% nonfat dry milk in Tris-buffered saline and Tween 20 ...
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bioRxiv - Neuroscience 2021Quote: ... QPCR was performed to determine Piezo2 knockdown effects by transfection of piezo2-shRNA into N2A cells using IQ Syber Green supermix (Bio-rad, Hercules, CA, USA) on a Bio-rad CFX96 Real-time PCR Machine and specific intron-spanning primers to quantify the cDNA levels of Piezo2 (Table 1) ...
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bioRxiv - Cell Biology 2019Quote: Sequence-confirmed constructs were linearized and transformed into the macronucleus of WT cells by biolistic bombardment using a PDS-1000 particle bombarder (Bio-Rad, Hercules, CA, USA) (Bruns and Cassidy-Hanley ...
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bioRxiv - Neuroscience 2021Quote: A multiplex biometric immunoassay containing fluorescent dyed microbeads was used to measure cytokines in the cell culture supernatant (Bio-Rad Laboratories, Hercules, CA, USA). The following cytokines were quantified ...
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bioRxiv - Cancer Biology 2019Quote: ... for approximately 45 min using the Mini-PROTEAN Tetra Cell and PowerPac™ 300 using tris-glycine SDS running buffer (all Bio-Rad, Watford, UK). Protein ladder SeeBlue™ Plus 2 Pre-stained Protein Standard (Invitrogen™ through Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The resuspended cDNA was divided into six aliquots and each aliquot was transformed into ElectroMax™ DH10B™ T1 phage resistant competent cells in a separate vial using a Gene pulsor Xcell™ electroporation system (Bio-Rad) as mentioned in the kit’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... Native-SDS PAGE was carried out as follows: samples were subjected to a Bio-Rad Protean II xi slab cell (Bio-Rad, Richmond, CA, USA), with a discontinuous buffer system (Laemmli ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Neuroscience 2022Quote: We prepared cellular extracts by a single-step lysis method.75 We suspended harvested cells in SDS-PAGE sample buffer [Laemmli sample buffer (Bio-Rad, Cat# 161-0737)] before boiling for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on 10%–12% SDS polyacrylamide gels by electrophoresis and transferred onto nitrocellulose membranes using a Trans-Blot SD Semi-Dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% skim milk for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... Separation in the second dimension was accomplished using an 10% SDS-PAGE in a Protean II xi 2-D Cell (Bio-Rad, Hercules, CA, USA) at 50 mA until the bromophenol blue reached the bottom of the gel ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred from polyacrylamide gel to polyvinylidine difluoride (PVDF) membranes as described by (Towbin et al., 1979) using a Trans-blot® electrophoretic transfer cell (Bio-Rad, city, country). The PVDF membrane with transferred proteins was blocked overnight at 4°C in blocking solution (5% (w/v ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Microbiology 2021Quote: ... 50 μl of cells were mixed with PCR products and transferred to a precooled 2 mm gap electroporation cuvette (Bio-Rad Laboratories, Hercules, CA). A BTX Gemini X2 instrument was used for electroporation with the following settings ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins or whole cell lysates were separated by SDS-polyacrylamide gel electrophoresis on a 4-20% gradient gel (Bio-Rad Laboratories, Hercules, CA) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: We mixed 500 μg of extracted cell lysates and 100 μL of patients’ sera with 100 μL of SureBeads™ Protein G Magnetic Beads (Bio-Rad, Hercules, CA) and added phosphate-buffered saline containing 0.2% of Tween 20 (PBS-T ...
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bioRxiv - Immunology 2020Quote: ... The cellular localization of EqPD-1-EGFP and EqPD-L1-EGFP was then confirmed using the ZOE Fluorescent Cell Imager (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... 30 μl were loaded and resolved on 6% sodium dodecyl sulfate (SDS) gels and transferred onto nitrocellulose membranes with Mini-PROTEAN III and Mini Trans-Blot cells (Bio-Rad, Hercules, CA, USA), respectively ...
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bioRxiv - Microbiology 2022Quote: ... the red fluorescent intensities of the microchambers on each device were examined to determine the MIC using a fluorescent microscope (ZOE™ Fluorescent Cell Imager system; Bio-rad, Hercules, California). For slow growth samples where the MIC was not clear after 4 h incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred to PVDF transfer membrane (IPVH00010 Immobilon®-P; MilliporeSigmaTM, Burlington, USA) overnight at 10 mA in a cold room (Mini trans-blot cell; Bio-Rad, Hercules, USA). The membrane was blocked for non-specific sites and incubated with anti-HvHsfA6a in 1:2000 dilutions overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated on an 8-12% SDS-polyacrylamide gel and blotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), The membrane was incubated overnight at 4°C with primary Abs ...
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bioRxiv - Biochemistry 2023Quote: ... Total protein concentrations of the cell extracts were determined using the Bio-Rad Protein Assay Kit according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA) using BSA as standard.
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bioRxiv - Cell Biology 2023Quote: ... the protein was transferred to the 0.2 μm nitrocellulose membrane using a semi-dry transfer system (#1703940, Trans-Blot® SD Semi-Dry Transfer Cell, Bio-Rad, Hercules, CA, USA) for 55 min at 25 V using 1x transfer buffer (Pierce Western Blot Transfer Buffer 10x ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were obtained from MAYV- or UNAV-infected HMC3 or HBEC-5i cells in Laemmli buffer with 10% dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were fractionated in SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: Vaginal mucosa cells and splenocytes were analyzed by flow cytometry using the following antibodies: mouse anti-guinea pig CD8 (clone MCA752F, Bio-Rad Laboratories, Hercules, CA), mouse anti-guinea pig CD4 (clone MCA749PE ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cell protein lysate was pre-cleared with 25 μL of SureBeads TM Protein G magnetic beads (BIO-RAD, Catalog number – 1614023) for 1 hour at 4 ºC and the beads were removed three times using a magnetic stand ...
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bioRxiv - Biophysics 2023Quote: ... then transferred onto PVDF membrane (Hybond-LFP, Amersham BioSciences, Little Chalfont, Buckinghamshire, United Kingdom) by using a semi-dry blotter (Trans-Blot Cell, Bio-Rad, Hercules, CA, USA). To differentiate the truncated product from T2 ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.