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Citations for Bio-Rad :
2551 - 2600 of 2723 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... the samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using the mini-PROTEAN II cell electrophoresis system (Bio-Rad, USA). The proteins were denatured in 2× loading buffer at 100°C for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from freshly isolated or cultured cells using the Aurum Total RNA Mini Kit (732-6820, Bio-Rad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were loaded on 4-12% Bis-Tris gel and separated by SDS-PAGE on the Criterion system (Bio-Rad Laboratories), and electrophoretically transferred to nitrocellulose membrane ...
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bioRxiv - Microbiology 2022Quote: ... or in-house prepared 15% polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories). The membranes were incubated in blocking buffer [5% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... in transfer buffer (50 mM Tris, 40 mM glycine, and 20% methanol) using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) at 25 V for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Electrophoresis was carried out for 1 hour at 90 V using an electrophoresis system (mini–DNA SUB CELL, Bio-Rad, Mississauga, Canada). The DNA fragments were visualized in a Gel Doc XR+ Gel Documentation System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Equal total protein was loaded from each of the indicated cell lysates and run on a Criterion™ XT 4-12% Bis-Tris protein gel (Bio-Rad). Protein was transferred at 4°C to nitrocellulose membrane for 2 hours at 50V ...
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bioRxiv - Biochemistry 2021Quote: ... Harvested cells were lysed in TNI lysis buffer and 10 μg of protein (as measured using Bio-Rad protein assay dye reagent) was aliquoted and precipitated via methanol/chloroform precipitation as described below ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed and homogenized as described elsewhere.30 Protein concentration was determined using a DC protein assay kit (Bio-Rad, 5000116). 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were resuspended in MACS buffer (PBS +2% FBS +2mM EDTA) and counted using trypan blue with the TC20™ Automated Cell Counter (Bio-Rad). In some cases ...
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bioRxiv - Plant Biology 2019Quote: ... Ten microliters of the cell extract was subjected to 4-20% sodium dodecyl sulfate polyacrylamide electrophoresis gels (SDS-PAGE, Bio-Rad, CA). SDS-PAGE containing proteins were transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... SDS-PAGE was carried out by using 4-20% SDS CriterionTM TGXTM pre-cast gels in a cell separation unit Criterion DodecaTM (BIO-RAD, Australia), in the buffer containing Tris-glycine-HCl at 200 V for 100 min ...
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bioRxiv - Microbiology 2019Quote: The purity of the proteins was checked using SDS-PAGE electrophoresis in a vertical electrophoretic mini-cell unit (Bio-Rad, Hercules, CA), in Tris-glycine running buffer (25mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... Chlorophyll amounts and cell numbers were determined according to the method of (Porra et al., 1989) and using a TC20 automated cell counter (Bio-Rad Laboratories), respectively ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were run under reducing conditions on an Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gel (BioRad) and imaged using a ChemiDoc imaging system (Biorad) ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... either by wet blot in transfer buffer (25 mM Tris, 192 mM glycine, 20 % ethanol) using Mini Trans-Blot® Cell chambers (Bio-Rad) or by semi-dry blot using the Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from RNA isolated from the cell lysates using a iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA), and qRT-PCR was conducted as described previously (21 ...
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bioRxiv - Genomics 2021Quote: ... Nuclei from the 0hr myoblast sample and 72hr sample in single-nucleus suspensions were counted on a TC20 Automated Cell Counter (Bio-Rad, 1450102), and ∼4 million were filtered through a 40 μm strainer into 15 mL polypropylene falcon tubes ...
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bioRxiv - Immunology 2021Quote: ... 10 μg of total cell lysates proteins were separated by electrophoresis on 10% SDS polyacrylamide gels (previously prepared following the Bio-Rad protocol) and transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysates were separated by electrophoresis on pre-cast 4-15% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... The product from the final elution and whole cell lysate supernatant were subjected to silver staining using Silver Stain Plus Kit (BIO-RAD, # 1610449) to check pull down efficiency and purity ...
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bioRxiv - Evolutionary Biology 2021Quote: RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Cat# 74104) and cDNA was prepared using the iScript cDNA Synthesis Kit (Biorad, Cat# 1708890). Assays were done in triplicate using Express SYBR GreenER master mix (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... were boiled at 95⁰C for 5 min with Roti load reducing loading buffer (Carl Roth, Karlsruhe, Germany) and subjected to SDS-PAGE followed by transfer (Semi-Dry Transfer Cell, Biorad, Munich, Germany) to a PVDF membrane (Merck Millipore ...
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bioRxiv - Biophysics 2019Quote: ... GFP+ cells were sorted from an unbiased FSC-SSC gate on a Sony SH800 cell sorter into 384-well hard-shell PCR plates (Bio-Rad HSP3901) containing .4 μl of lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... and 40ng of RNA for lung or 5-10ng of RNA for cells was converted to cDNA with iScript (Biorad, Hercules, CA). Real-time PCR was done on the cDNA with SYBR green master mix (Biorad ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were blotted onto 0.45 µm nitrocellulose membrane (Amersham) overnight at a constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA).
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bioRxiv - Synthetic Biology 2022Quote: ... The solid cultures were grown at 30ºC without shaking for 48 h and pictures of cell plates were taken using the colorimetric mode of Chemidoc Touch Imaging Systems (Bio-Rad, USA). All halo assays were conducted in three biological replicates.
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bioRxiv - Microbiology 2022Quote: ... intrahepatic cccDNA values were normalized by the cell number measured by the hRPP30 copy number variation assay (Bio-Rad, Pleasanton, California, USA)68 ...
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bioRxiv - Microbiology 2023Quote: ... 100 µL of the electrocompetent cells were aliquoted into pre-chilled 0.2 cm gap Gene Pulser Electroporation Cuvettes (Bio-rad Laboratories #1652086) and kept on ice ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Amersham, Cytiva, Marlborough, USA) using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) for 1.5 h at 1.5 mA/cm2 ...
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bioRxiv - Plant Biology 2024Quote: Protein samples of whole cell extracts (5 μg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, US) and blotted onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein(s) in the gel were transferred to a PVDF membrane using a Mini-PROTEAN Tetra Cell (Bio-Rad, United States). After blocking the membrane with 3% BSA at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% Acrylamide:Bis Acrylamide) for 1 hour and transferred to a nitrocellulose membrane (0.45µm) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysates were separated on SDS/PAGE and proteins on the gels were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% skim milk diluted in TBST for 1 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... were loaded onto a 12.5 % SDS-PAGE gel and separated at 160 V for 70 min in a mini-protean tetra cell (Biorad, Hercules, CA, USA) using Tris-glycine-SDS running buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Histone samples (4 μg protein/well) or whole cell lysates (see above, 40 μg protein/well) were separated on precast gels (4-20%, BioRad, CA, USA) and transferred to 0.2 μm nitrocellulose membranes (Turbo transfer pack ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments was separated on 20% denaturing acrylamide gel (5% crosslinker, 7 M urea, 1X TBE buffer) using Criterion™ cell apparatus (Bio-Rad) at 300 V for 40 to 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 15,000 x g for 1 min in a tabletop centrifuge to pellet cell debris and the supernatant was diluted 1:10 in sterile ddH2O for a 96-well microplate Bradford microassay (Bio-Rad 5000201) to estimate the amount of leaked protein ...
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bioRxiv - Cancer Biology 2023Quote: ... 40–50 μg of cell lysate were loaded in precast SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) gels (Biorad #456-8096) and then transferred onto nitrocellulose membrane by Trans-Blot Turbo Transfer Pack (Biorad #170-4157) ...
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bioRxiv - Microbiology 2023Quote: ... intrahepatic cccDNA values were normalized by the cell number measured by the hRPP30 copy number variation assay (Bio-Rad, Pleasanton, California, USA)[53] ...
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bioRxiv - Microbiology 2023Quote: ... donghuensis P482 strains were obtained by transforming the bacteria cells with pPROBE-GTkan vector (Miller et al., 2000) by electroporation (Gene Pulser Xcell; Bio-Rad, USA), as described in (Krzyzanowska et al. ...
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bioRxiv - Microbiology 2023Quote: Recombinant VP1 protein and lysate prepared from Vero E6 cells infected with EMCV strain SING-M105 were separated by SDS-PAGE (Bio-Rad, USA) and the proteins transferred onto polyvinylidene fluoride membrane (Immobilion-P ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells in the top chamber were removed and those on the bottom of the transwell were visualised at 20x magnification under the ZOE™ Fluorescent Cell Imager (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... The supernatants of the cell lysates were normalized for total protein concentration according to Bradford assay (#5000006, Bio-Rad, Hercules, CA, USA), mixed with SDS loading dye ...
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bioRxiv - Cell Biology 2023Quote: ... Depletion and/or induced expression of RB was monitored by western blot analysis of whole cell lysates prepared using 2x Laemmli sample buffer (Bio-Rad #1610737) supplemented with 2-mercapto-ethanol (Fisher Scientific #BP176-100 ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA coated with gold particles was then bombarded into rice callus cells using the PDS-1000 HeTM system (BioRad, Hercules, CA) at the University of Missouri plant transformation facility ...
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bioRxiv - Cancer Biology 2023Quote: ... was evaluated daily for 5 consecutive days by trypan blue staining with automated cell counting using a TC20™ counter (Bio-Rad). At day 0 ...
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bioRxiv - Systems Biology 2023Quote: ... 1.5 μl of concentrated ligation reactions was mixed with 25 μl of competent cells and pipetted into a pre-chilled 0.1 cm Gene Pulser Cuvette (Bio-Rad, Hercules, California). Each mixture was electroporated using a Gene Pulser Xcell (BioRad ...