Labshake search
Citations for Bio-Rad :
2601 - 2650 of 2723 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... The cell suspension was collected and centrifuged at 4°C for 15 minutes at 10,000g and cell protein extracts were stored at −80°C or brought forward for total protein quantification using Bradford assay (Bio-Rad; USA). Cell protein samples were suspended in 2x Laemmli buffer (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 μg of cell lysates were separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), transferred to a PVDF membrane for analysis using anti-FLAG (Sigma Aldrich #F3165 ...
-
bioRxiv - Biochemistry 2024Quote: ... an equal number of cells were loaded and run on an SDS-PAGE gel (Bio-Rad #4561086: 4-15 Mini-PROTEAN TGX) along with Precision Plus Protein Dual Color Standards (BIO-RAD ...
-
bioRxiv - Physiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes using a semi-dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% non-fat dry milk in TBS-Tween for 1 h at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in RPMI complete media at 1×106 cells/ml and stimulated with 5 µg/ml of anti-Igκ plus anti-Igλ F(ab’)2 antibodies (BioRad, Table 2) for the indicated time at 37°C ...
-
bioRxiv - Immunology 2024Quote: RNA was isolated (ZymoResearch R2050) from the infected Hsu cells at various passages and 1 μg of RNA was used to prepare cDNA (BioRad 170-8891). The cDNA was used as the template for various PCR reactions targeting the 4 genes of interest - E ...
-
bioRxiv - Plant Biology 2020Quote: Temperature sensitive mutant cells were transformed with the linearized plasmid by electroporation (500 V, 50 μF, 800□Ω) using GenePulser Xcell (Bio-Rad, CA). The cells were recovered in TAP containing 40□mM sucrose ...
-
bioRxiv - Biophysics 2021Quote: ... and transfected with Fluorescence Multiplex-Host Cell Reactivation (FM-HCR) reporter plasmids using the Gene Pulser MXCell Plate Electroporation System (Bio-Rad, #165-2670) using conditions 260 V ...
-
bioRxiv - Cell Biology 2020Quote: ... 40 μg of whole cell protein lysate were applied to 4%-15% Mini-PROTEAN TGX precast protein Gel (Bio-Rad, Cat No. 4561084) with electrophoresis and then transferred to a nitrocellulose membrane ...
-
bioRxiv - Cell Biology 2020Quote: ... Supernatants were collected and stored at −80°C and cells were lysed in 1x Laemmli Buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) for further analysis.
-
bioRxiv - Molecular Biology 2019Quote: ... rab6-TAG-nluc and rab6-CAG-nluc were separately cloned into vector pVALIUM10-roe using Gateway recombination. RNA extracted from S2 cells (a kind gift from Dr. Gong Cheng) was used to synthesize cDNA (Bio-Rad, cat#1708890). Coding sequences of rps20 ...
-
bioRxiv - Genomics 2019Quote: ... Total HIV-1 DNA content of CD4 T-cell DNA isolates was measured by digital droplet PCR (ddPCR, QX200 platform, Bio-Rad, Temse, Belgium), as described by Rutsaert et al.56 The DNA was subjected to a restriction digest with EcoRI (Promega ...
-
bioRxiv - Physiology 2019Quote: Cell or tissue protein lysates were separated by electrophoresis on pre-cast 4-15% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (all purchased from Bio-Rad Laboratories), blocked in non-fat milk and incubated overnight at 4°C with primary antibody for HILPDA (Santa-Cruz Biotechnology) ...
-
bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
-
bioRxiv - Biochemistry 2020Quote: Cell or tissue protein lysates were separated by SDS-PAGE on pre-cast 8-16% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (all purchased from Bio-Rad Laboratories), blocked in non-fat milk and incubated overnight at 4°C with primary antibody for HILPDA (1:750 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The lysates were centrifuged at 12,000g for 20 mins to remove cell debris and protein concentration in supernatants was determined using DC™ Protein Assay (Bio-Rad, #5000111). Twenty μg of proteins from L ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were recovered from the resulting BAL fluid by centrifugation (500 g, 20 min, 4°C) and counted using an automated cell counter (TC20; Bio-Rad, Hercules, CA). All animal care procedures and experiments were approved by the University of Arizona Animal Care and Use Committee (Approval #13-490).
-
bioRxiv - Plant Biology 2020Quote: Proteins were transferred from an SDS-polyacrylamide gel to a polyvinylidene difluoride (PVDF) membrane by electroblotting using a Trans-Blot SD transfer cell (Bio-Rad, www.bio-rad.com) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
-
bioRxiv - Cell Biology 2021Quote: ... Similar amount of total cell lysate were fractionated through a 10% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Lab, California, USA). The proteins transferred to the membrane were visualized by Ponceau solution stain 0.3% for 5 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... The proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane applying 20 mA for 20 minutes in a semi-dry transfer cell (Bio-Rad Laboratories, USA). The membrane was blocked in 0.5% powdered milk in TBS-T buffer at 4°C overnight ...
-
bioRxiv - Biochemistry 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
-
bioRxiv - Neuroscience 2021Quote: Protein lysates from SH-SY5Y cells were quantified (bicinchoninic acid protein assay, Pierce) and then electrophoresed in 4—12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad) in 1X TGS Tris/Glycine/SDS Buffer (10X TGS Buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
-
bioRxiv - Bioengineering 2021Quote: ... All plasmids in this study have been transformed into cells using electroporation with the Bio-Rad Micropulser™ (Bio-Rad Laboratories, Hercules, CA) and were created using Gibson Assembly[51] using the Gibson Assembly Master Mix (New England Biolabs ...
-
bioRxiv - Biophysics 2021Quote: ... loaded them into polyacrylamide gel wells and ran them for approximately 45 minutes at 190 V in a mini-PROTEAN tetra vertical electrophoresis cell (Bio-Rad Laboratories, Inc). We transferred the protein bands onto PVDF membranes (170-4156 ...
-
bioRxiv - Biochemistry 2020Quote: ... EV proteins were electrophoresed on a 12.5 % polyacrylamide gel (50V, 30 min) to obtain a single band (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France). The gel was stained with Coommassie Blue and the single band of proteins was excised and subjected to in-gel digestion with bovine trypsin (Roche Diagnostics GmbH ...
-
bioRxiv - Microbiology 2019Quote: ... Purified protein was denatured and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a Criterion™ Cell and transferred to a nitrocellulose membrane in a Criterion™ Blotter (Bio-Rad) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Equal amounts of protein from cell lysates were separated by SDS PAGE and transferred onto a nitrocellulose membrane by a semi-dry transfer method (Trans Blot® SD Semi-Dry Transfer cell, Bio-Rad, USA). Membranes were blocked with 5 % milk solution before incubation with primary antibodies at 1:1000 dilution unless otherwise stated ...
-
bioRxiv - Physiology 2020Quote: ... The following antibodies were selected and tested on half of each cornea: anti-CD31 mouse monoclonal IgG1 targeting vascular endothelial cells (BioRad, Kidlington, Oxford, England), anti-Neutrophil rabbit polyclonal IgG targeting neutrophils (LifeSpan BioSciences ...
-
bioRxiv - Neuroscience 2020Quote: 15 μg of total protein extract from purified proteins were separated by native electrophoresis using a Tetra cell (Bio-Rad; Hercules, CA, USA), in 12% polyacrylamide separation gel and a 4% polyacrylamide stacking gel in non-denaturing conditions ...
-
bioRxiv - Immunology 2019Quote: Intestinal epithelial cells lysate (20 µg per lane) were separated by SDS-PAGE through 4%-20% Mini-PROTEAN® TGX™gel (BIO-RAD), transferred to nitrocellulose membranes ...
-
bioRxiv - Biochemistry 2020Quote: Protein lysate from DENV infected and non-infected Huh7 cells (10 µg of protein as measured using Bio-Rad protein assay dye reagent) was aliquoted and purified using methanol/chloroform precipitation ...
-
bioRxiv - Cancer Biology 2021Quote: ... and gene expression of MCL-1 in the parental and MCL-1 deleted cells were verified by qPCR performed using the iTaq™ Universal SYBR Green Supermix (BioRad, California, USA), according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: The protein extracts from the cells (40 μg) were subjected to electrophoresis in SDS-PAGE on 4–15% precast protein gels (Bio-Rad, Hercules, CA), separated and transferred to polyvinylidene fluoride (PVDF ...
-
bioRxiv - Developmental Biology 2021Quote: 2 μg of total protein derived from meiotic or post-meiotic germ cell populations was loaded onto a Mini-PROTEAN TGX gel (Bio-Rad, 456-8093) and separated by SDS-PAGE for 1 hour at 150V ...
-
bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
-
bioRxiv - Microbiology 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
-
bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
-
bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
-
bioRxiv - Genetics 2022Quote: ... and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc., CA, USA). Membranes were blocked with 5% non-fat dry milk in PBST for 2h ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR products (700 ng) were introduced into electrocompetent cells of JW0452 carrying pKD46 by electroporation using a Gene Pulser XcellTM (BioRad, Hercules, CA, USA). The occurrence of recombination at the expected site was confirmed by PCR using primers CAT-584 and BW25113_2815723f (Table 2) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... cervicornis sperm samples from 2015 were brought to a final concentration of 3 x 107 cells ml-1 after the addition of Cell Suspension Buffer and 2% agarose using the Bio-Rad CHEF Genomic DNA Plug Kits (Bio-Rad, Hercules, CA). Genomic DNA plugs were processed according to the manufacturer’s protocol and stored at 4 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
-
bioRxiv - Microbiology 2024Quote: ... Then 300 ng of plasmids were transformed into 100 μl of competent cells using the bacteria program on the MicroPulser Electroporator (Bio-Rad, CA, USA). After 1 h recovery culture at 37°C in 500 μl of LB ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10-20 µl of the cell lysates were separated on 4-15% Mini-PROTEAN® TGX™ Stain-FreeTM Protein Gels from Bio-Rad and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
-
bioRxiv - Cell Biology 2024Quote: Total protein lysates were prepared from the cells followed by protein quantification using the protein DC assay kit (Bio-Rad, Cat # 5000-001) as described previously (28) ...
-
bioRxiv - Genetics 2024Quote: ... The percentage of targeted alleles within a cell population was measured with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7; Bio-Rad, Hercules, CA, USA) using the following reaction mixture ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 µL of the first PCR amplification product was then used for analysis on a 2% ethidium bromide-stained agarose gel (120 minutes at 80V in a Mini-Sub® Cell GT gel chamber connected to a PowerPac™ HC (Bio-Rad) power supply ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in cell lysates were measured using a RC DC Protein Assay Kit (cat. no. 50000119; Bio-Rad Laboratories, Hercules, CA, USA). Immunoblotting was carried out using standard procedures ...