Labshake search
Citations for Bio-Rad :
2451 - 2500 of 2723 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... tabacum) BY-2 suspension-cultured cells and biolistic bombardment using a PDS-1000/He biolistic particle delivery system (Bio-Rad Laboratories) were performed as previously described (Banjoko and Trelease ...
-
bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
-
bioRxiv - Molecular Biology 2022Quote: ... A final concentration of 0.1 μg/mL DAPI was then added for 10 mins and digital images were captured using ZOE Fluorescent Cell Imager (Bio-Rad). For negative controls ...
-
bioRxiv - Molecular Biology 2022Quote: ... 25 µg total RNA each from WT and rad6Δ cells was resolved on 15% TBE-Urea polyacrylamide gel (Bio-Rad, #3450091). The RNAs were then transferred onto positively charged nylone membrane (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... 30 μg of whole-cell lysate or IP elutions were resolved on 4 to 15% mini-PROTEAN TGX gels (Bio-Rad). Transfers to PVDF membranes (Bio-Rad ...
-
bioRxiv - Microbiology 2022Quote: All native electrophoresis gels were run in a Tetra Vertical Electrophoresis Chamber (Mini-PROTEAN® Tetra Cell, Bio-Rad, CA, USA). Recombinant PNPase (100 pmols ...
-
bioRxiv - Cell Biology 2022Quote: ... The resulting lysate was resolved on SDS-PAGE gel and protein was blotted to nitrocellulose membrane (Li-COR #926-31902) with mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for an hour in Odyssey Blocking Buffer (TBS ...
-
bioRxiv - Immunology 2022Quote: ... Even amounts of each protein sample were separated on a 4–16% Mini-PROTEAN TGX Stain-Free Precast Gels using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
-
bioRxiv - Systems Biology 2022Quote: ... Equal amounts of each protein sample were separated on 4% to 16% Mini-Protean TGX stain-free precast gels using the Mini-Protean Tetra cell electrophoresis system (Bio-Rad). Upon the completion of electrophoresis ...
-
bioRxiv - Biophysics 2022Quote: ... Both this sample and the total cell protein sample were mixed 1:1 with 2x loading buffer: 50% 4x Laemmli Sample Buffer (Bio-Rad), 30% 100 mM DTT ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were grown at 30°C for 3-6 days and images captured using a Chemidoc XRS+ imager (BioRad, Hercules, CA). All images were evenly adjusted in Photoshop (Adobe Systems Incorporated ...
-
bioRxiv - Molecular Biology 2024Quote: Cell cultures at the log phase of growth were collected and checked for cell number and viability using TC20 automated counter (Bio-Rad). Samples with >90 % viability were processed in order to prepare single cell suspensions and diluted into the target numbers plus the multiplet rate (1000 cells plus 0.8 % per library) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Total protein content of cell free BALF was determined using the DC Protein Assay Kit (Cat # 5000111; BioRAD, Inc, Hercules CA) with bovine serum albumin as a standard according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2024Quote: ... the proteins were blotted onto methanol-activated polyvinylidene difluoride (PVDF) membranes (0.2 µm) with a Trans-Blot SD Semi-Dry Transfer Cell system (Bio-Rad Laboratories). After blotting ...
-
bioRxiv - Molecular Biology 2024Quote: ... a total of 20 µg whole cell lysate was resolved in a 10 % precast Mini-PROTEAN TGX Stain-Free gel (Bio-Rad). At the end of the run ...
-
bioRxiv - Neuroscience 2024Quote: ... The samples were separated on a 15% Tris-Glycine gel with a 4% stacker using a Tris/Glycine/SDS buffer and transferred overnight onto an Immobilon-FL Transfer Membrane using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for one hour (0.5% Non-Fat Dry Milk in PBS ...
-
bioRxiv - Microbiology 2023Quote: ... run on a 12% SDS‒PAGE gel and transferred onto a PVDF membrane using a Trans-Blot Turbo electrophoretic transfer cell (Bio-Rad) at 25 V for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
-
bioRxiv - Cancer Biology 2023Quote: ... For Western blotting equal amounts (up to 80 μg) of total cell lysates were loaded on SDS–polyacrylamide gel electrophoresis system (Bio-Rad) and transferred on nitrocellulose membrane which was proceeded for immunoblotting to detect specific proteins ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 50 μl of competent cells were mixed with 500 ng of the PCR fragment in an ice-cold 0.2 cm cuvette (Bio-Rad Inc.). Cells were electroporated at 2.5 kV with 25 mF and 200 Ω ...
-
bioRxiv - Bioengineering 2023Quote: ... For detection, we used an anti-rabbit HRP-coupled secondary antibody (1:5000, Cell Signalling) and Western ECL substrate (#1705061, Bio-Rad. As a loading control ...
-
bioRxiv - Cell Biology 2023Quote: ... proteins incubated with Mal-B were electrotransferred to PVDF membranes using a Trans-blot SD semi-dry transfer cell (Bio-Rad). Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v ...
-
bioRxiv - Genetics 2024Quote: ... Forty micrograms of whole-cell lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Bio-Rad). The membranes were blocked with 5% nonfat dry milk in Tris-buffered saline with 0.05% Tween 20 (TBST ...
-
bioRxiv - Microbiology 2024Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
-
bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
-
bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
-
bioRxiv - Bioengineering 2023Quote: ... base editor cells were kept in the dark for 48 hours after light exposure and fluorescence micrographs were taken on a ZOE Fluorescent Cell Imager (Bio-Rad). To achieve optogenetic base editing of an endogenous gene ...
-
bioRxiv - Microbiology 2023Quote: ... and the gel was run at 180 V for about 35 minutes (Mini-PROTEAN electrophoresis cell and PowerPac power supply, Bio-rad). Coomassie brilliant blue (Bio-Safe Coomassie stain ...
-
bioRxiv - Microbiology 2023Quote: ... Change in bacteria and host cell gDNA copies was assessed by qPCR using iTaq universal SYBR green Supermix (Bio-Rad; 1725125) in duplicate reactions ...
-
bioRxiv - Microbiology 2022Quote: ... Virus pellets and clarified cell lysates were then analyzed by Western blotting using a nitrocellulose membrane and wet transfer (350 A, 75 min, Bio-Rad). Western blots were developed with 1:2,000 goat anti-gp120 polyclonal antibody (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... such that a fluorescent construct can be used as an expression reporter for cells co-transfected with a non-fluorescent construct.1 Cells were electroporated at 280 V and 950 μF using Gene Pulser X (Bio-Rad), then immediately resuspended in 6 ml medium and cultured in MatTek dishes (2 ml/dish ...
-
bioRxiv - Cell Biology 2023Quote: ... 30 µg protein extract was loaded onto a 15% Bis-Tris polyacrylamide gel for electrophoresis then transfer to a PVDF membrane using a Mini Trans Blot Cell (Bio-Rad). Membranes were blocked for 1 h at 23°C in 4% milk/PBST (0.1% Tween-20 in PBS) ...
-
bioRxiv - Cancer Biology 2023Quote: Formalin-fixed and paraffin-embedded 5μm sections were stained with hematoxylin and eosin (HE), DCT (Pep8h), SOX10 (clone EP268, Cell Marque, 383R-15) or CD3 (clone CD3-12, BioRad, MCA1477) antibodies using standard immunohistochemistry methods ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfer was performed for 50 min and a constant voltage of 120 V on a Mini Trans-Blot Cell (Biorad R) system ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The purity and size of the proteins were validated on an SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at 4° C.
-
bioRxiv - Cell Biology 2023Quote: ... whole cell lysates were quantified and aliquots of samples were resolved on a Criterion TGX Stain-Free precast gel (Bio-Rad). The membrane was blocked in 1XPBS + 0,1% Tween (1XPBS-T ...
-
bioRxiv - Physiology 2022Quote: ... Images were captured for each well in a 24 well plate using a cell imager equipped with the correct LED lights (ZOE, Bio-Rad). Fluorescence intensity was analyzed and quantified using ImageJ software(22 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were then transferred to a nitrocellulose membrane (GE Water and Process Technologies) using a wet transfer protocol and a Mini Trans-Blot Cell (Bio-Rad) transfer apparatus at 4C ...
-
bioRxiv - Immunology 2023Quote: ... Bovine CD4+ T cells were isolated from PBMCs by magnetic-activated cell sorting (MACS) using a mouse anti-bovine CD4 antibody (clone CC8, Bio-Rad), anti-mouse IgG MicroBeads (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2023Quote: ... Denatured lysates were analyzed by SDS-PAGE and gels were transferred to PVDF membranes using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked in 5% non-fat dry milk in TBS containing 0.1% Tween-20 (TBST ...
-
bioRxiv - Molecular Biology 2023Quote: ... single yeast cells were gated on a forward-scatter area (FSC-A) by side-scatter area (SSC-A) plot around the clustered population (P1) on a ZE5 Cell Analyzer (Bio-Rad). P1 was then gated on a side-scatter width (SSC-W ...
-
bioRxiv - Molecular Biology 2023Quote: ... Western blot analysis was accomplished through SDS-PAGE of cell lysates or affinity-resin eluates in 1X laemmli loading-buffer (Bio-Rad) containing β-mercaptoethanol (1/10 in 4X buffer ...
-
bioRxiv - Developmental Biology 2023Quote: ... 0.1 µg RNA (sorted cells) or 1 µg RNA (tissues) was used to prepare cDNA with the iScript™ cDNA synthesis kit (Biorad) with the following protocol ...
-
bioRxiv - Developmental Biology 2023Quote: Cell lysates from ITFG1-Myc-expressing MDA-MB-231 and non-expressing control MDA-MB-231 cells were separated in a 4-20% SDS-PAGE gel (Bio-Rad). The gels were stained with Imperial Protein Stain (Thermo Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... Purified CreS controls and equivalent OD600 nm units of cleared cell lysates by centrifugation were resolved on a 4-20% SDS-polyacrylamide gel (Bio-Rad). In this step ...
-
bioRxiv - Systems Biology 2023Quote: ... by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany, 1658005EDU). Proteins were transferred to a nitrocellulose membrane (Carl Roth ...
-
bioRxiv - Genomics 2023Quote: ... from cell preparations were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a PVDF membrane (Bio-Rad) using a Trans-Blot Turbo™ transfer system (Bio-Rad) ...
-
bioRxiv - Microbiology 2023Quote: Total protein extracts (5x106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
-
bioRxiv - Synthetic Biology 2023Quote: ... we electroporated a small fraction of the assembly reaction into inhouse DH10B cells using 1 mm cuvettes and a micropulser (Bio-Rad) set at 1.8 kV ...
-
bioRxiv - Bioengineering 2022Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...