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Citations for Bio-Rad :
1901 - 1950 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The amount of protein in the biomass was determined by the Quick StartTM Bradford Protein Assay (BioRad, Mississauga, ON, Canada).
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bioRxiv - Molecular Biology 2021Quote: ... was added to the samples according to the manufacturer’s instruction and equivalent amounts of protein (30 μg as measured using the DC Protein Assay Kit (Bio-Rad) and proteins were separated on a 4 to 12% on a 12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufacturer’s instruction and measured using the Eon High Performance Microplate Spectrophotometer (BioTek ...
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bioRxiv - Immunology 2022Quote: ... Lysates containing 40 μg of proteins were analyzed by SDS-PAGE on 9-13% gels and proteins were transferred onto nitrocellulose membranes (BioRad). The blots were blocked in non-fat milk diluted in tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2022Quote: ... and the resulting supernatants were normalized by total protein concentration measured by the RC DC Protein Assay kit (Bio-Rad). Equal volumes of retinal lysate containing 10 μg total protein from each mouse genotype were subjected to SDS/PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... The molecular masses of the proteins were estimated relative to the electrophoretic mobility of a co-transferred prestained protein marker (Precision Plus Protein Standards Dual Colour; 161-0374; BioRad). Densitometry analysis of the bands was carried out used the Fiji software (open source ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 50 μL of 1X PBS and protein concentration was using The DC Protein Assay Kit (Biorad).
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bioRxiv - Molecular Biology 2022Quote: ... Total protein was determined directly on the membrane using the 4-15% Mini-PROTEAN TGX-Stain Free Protein Gel system (BioRad). Antibodies used for Western blotting analysis were ...
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bioRxiv - Neuroscience 2022Quote: 20ug of proteins from N2A cells or 10ug of protein from DRG were loaded to SDS-polyacrylamide precast gels (Biorad) and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2023Quote: Three biological exosome samples (20 μg/sample) were separated on 4-15% mini-protein TGX precast protein gels (Bio-Rad) and subsequently stained with Coomassie Blue ...
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bioRxiv - Molecular Biology 2022Quote: ... For the activity assay (see below) protein concentration was determined using Bio-Rad Protein Assay dye reagent concentrate (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The protein concentration of soluble cytoplasmic and membrane fractions was determined using the Bio-Rad Protein Assay (Bio-Rad Laboratories). Samples were boiled in 4X sample buffer ...
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bioRxiv - Physiology 2022Quote: ... lysates were centrifuged at top speed for 10 min and supernatant were collected and proteins were quantified using Protein DC assay (BioRad). Proteins were running and separated on 4%-12% Bis-Tris Pre-cast Gel (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... and 40ug of protein were loaded per lane on a Mini-PROTEAN TGX Pre-cast Protein gel (Bio-Rad, #4568094) with the Precision Plus Protein Dual Color Standards Ladder (Bio-Rad #1610374) ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations of the cellular extracts were determined using the DC Protein Assay (ref 5000112, Bio-Rad, Hercules, California, USA). Equal amounts of proteins (20 μg ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µg of total protein was loaded to AnykD™ Criterion™ TGX Stain-Free™ Protein Gels (BIORAD; #5678125). Transfer of the proteins from gel to the Immun-Blot® Low Fluorescence PVDF membrane with low fluorescent transfer buffer (BIORAD ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were assessed using the Protein Assay BCA kit (Nacalai Tesque) with bovine serum albumin (Bio-Rad, Hercules, CA) as a standard ...
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bioRxiv - Cell Biology 2023Quote: ... TAG content was normalized to total protein determined using a standard Bradford Protein assay (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Cell Biology 2023Quote: The protein concentrations of the cell lysates were determined using a Bio-Rad Protein Assay Dye Reagent (Bio-Rad, 5000006). Volumes of lysates containing the same amount of total protein were heated to 95°C for 5 minutes after addition of 1/4 volume of 4x SDS-PAGE Loading Solution (250 mM Tris ...
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bioRxiv - Systems Biology 2023Quote: ... and total protein abundance was normalized to 2 μg using the Detergentcompatible Protein Assay (Biorad, Hercules, CA, USA, Cat. # 5000111). 2 μg α-RelA antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...
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bioRxiv - Neuroscience 2022Quote: ... in nuclear and cytoplasmic reagents from the kit NE-PER as described by the manufacturer (Thermo Scientific).The cytoplasmic and nuclear supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Biochemistry 2023Quote: ... and flash-frozen in liquid nitrogen for storage at −80°C The protein concentration was determined using the DC protein assay (BioRad) per the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... 10-20 µg/lane total protein was electrophoresed on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) with SDS running buffer and transferred to Polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentration of protein in the eluted fractions was measured using the Bradford method (protein assay dye purchased form Bio-Rad). Fractions containing the proteoliposomes were combined and adjusted to 0.2 mg ml-1 protein concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration in the lysates was determined using the Bio-Rad Protein Assay Kit – BSA (Cat#5000002, Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... proteins were separated by SDS-PAGE using 4–20% Criterion™ TGX™ Precast Midi Protein Gels (Bio-Rad, #5671094), and then transferred to the low fluorescence background PVDF membrane (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... The protein samples were separated on 10% SDS-polyacrylamide gels and separated proteins were transferred on to nitrocellulose membrane (BIORAD) for immunodetection ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was used as a reference for the samples and was measured by Bradford protein assay from Bio-Rad.
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bioRxiv - Molecular Biology 2023Quote: ... Complexes were assembled for 30 minutes at 30 °C then passed through a nitrocellulose membrane (Amersham Protran 0.45 μm NC) to retain protein and protein-bound DNA using a 96-well Bio-Dot apparatus (Bio-Rad). Each well was then washed twice with 50 μl buffer containing 25 mM Tris-HCL pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants containing the protein extracts were transferred into fresh 1.5 ml tubes and protein concentration was measured by Bio-Rad Protein Assay Kit using BSA as protein standard (5000002 ...
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bioRxiv - Cancer Biology 2023Quote: ... Precision Plus Protein™ WesternC™ Blotting Standards and Precision Protein™ StrepTactin-HRP Conjugate (Bio-Rad, 1610376 and 1610381) were used ...
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bioRxiv - Bioengineering 2023Quote: The total protein concentration of the ECM digests was measured using a DC protein assay kit (Bio-Rad, Watford, UK) following the manufacturer’s instruction (n = 4) ...
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bioRxiv - Biochemistry 2023Quote: Proteins were separated by SDS-PAGE on 10% gels (20 μg protein/lane) and transferred to PVDF membrane (Bio-Rad) for 20 min at 0.1 A ...
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bioRxiv - Biochemistry 2023Quote: Proteins were separated by SDS-PAGE on 10% gels (20 µg protein/lane) and transferred to PVDF membrane (Bio-Rad) for 20 min at 0.1 A ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein concentrations of the supernatants were determined using DC™ Protein Assay Kit (Bio-Rad #5 000 112). Equal concentrations of protein (30 μg ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 0,5 %) and protein amount was measured using a Lowry based method (DC Protein Assay Kit 5000111, Bio-Rad). 10μg protein per sample were subjected for protein digestion and peptide purification using the iST label free sample preparation kit (PreOmics GmbH) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations of the cellular extracts were determined using the DC Protein Assay (ref 5000112, Bio-Rad, Hercules, California, USA). Equal amounts of proteins (20 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the eluted biotinylated proteins were collected and run on a 4-15% Mini-Protean TGX Precast Protein Gel (Bio-Rad) before being transferred to a nitrocellulose membrane and stained for biotin ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration in the conditioned media and lysates were determined by DC protein assay (Bio-Rad, Hercules CA, 500-011).
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed by spectrophotometry with PierceTM BCA Protein Assay Kit (ThermoFischer Scientific, cat # 23225) and proteins were separated on a 4% to 20% polyacrylamide gel (Biorad), and transferred to PVDF membrane (Bio-rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The total protein concentration for each supernatant was measured with a DC protein assay kit (5000113, 5000114, 5000115; Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The lysates were then centrifuged at 13,000 rpm for 15 min and protein amounts in the supernatants were measured by DC Protein assay (Bio-Rad). Proteins (10-20 μg ...