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Citations for Bio-Rad :
1751 - 1800 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Fifty micrograms of protein from each group was separated on 10% Tris-HCl gel using protein electrophoresis (BioRad, USA). The gels were stained in Coomassie brilliant blue overnight (o/n) ...
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bioRxiv - Microbiology 2019Quote: Protein samples were resolved on 4-20% Mini-PROTEAN TGX Precast Protein SDS-PAGE gels (BioRad Laboratories; Hercules, CA) and blotted on PVDF membranes using the Trans-Blot Turbo transfer system (BioRad) ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using the Bradford method with the Quick Start™ Bradford Protein Assay Kit (#5000202, BIORAD) according to the manufacturer’s instruction34 ...
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bioRxiv - Plant Biology 2019Quote: ... 16.000 x g) the protein concentration of the clear supernatant was determined by the Bradford protein assay (Bio-Rad). After SDS-PAGE (7.5%) ...
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bioRxiv - Neuroscience 2019Quote: ... The protein concentration of the samples was determined using the Bio-Rad DC protein assay (Bio-Rad, 500-0116). Samples were normalized with the same lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: Protein concentrations were determined by Bradford assay (Quick Start Bradford Protein Assay, Bio-Rad Laboratories Inc., Hercules, CA, USA) (Table S1) ...
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bioRxiv - Genetics 2021Quote: ... protease inhibitor cocktail) and protein concentrations were determined by Bradford protein assay (Bio-Rad Laboratories, Inc. Hercules, CA, USA) and absorbance was measured at 595 nm on the Tecan Infinite® 2000 PRO plate reader (Tecan ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were clarified by centrifugation and the protein concentration was determined using DC protein assay system (Bio-Rad Laboratories). Equal amount of protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... The concentration of the purified protein extracts was measured using the Bradford method (Protein Assay Dye Reagent, Bio-Rad). Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Cell Biology 2021Quote: Protein concentrations were determined by use of the Bradford assay (Bio-Rad protein assay kit II, Cat. No. 5000002). For immunoblotting ...
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bioRxiv - Plant Biology 2020Quote: The total protein in each extract was quantified using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), and 24 μg run in a 10% SDS-PAGE ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein concentrations in the cell lysates (mg/mL) were assayed using the Bradford protein assay (Bio-Rad, Hercules, CA) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were resolved by SDS-PAGE using 8–16% Mini-PROTEAN TGX Stain-Free protein gels (Bio-Rad) and transferred onto a PVDF membrane using a semi-dry apparatus (Trans-Blot Turbo Transfer System ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was collected and protein concentration was determined using the Bio-Rad protein assay kit (BIORAD, Hercules, USA) and bovine serum albumin as a standard ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μg of protein was loaded and run on 10% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Separated proteins were transferred onto Nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... the lysates were cleared by centrifugation at 16,000 × g for 15 min and protein concentrations were estimated using QuickStart Bradford Protein assay (BioRad). After combining the same amount of proteins from each label ...
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bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad).
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bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad). One unit of Acetyl-CoA Carboxylase activity is defined as the enzyme generates 1 nmol of PO43-per hour.
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bioRxiv - Cancer Biology 2022Quote: ... Diluted protein samples (15ug of protein per lane) were separated on pre-cast 4-20% gradient (Bio-Rad, 5671095) or 7.5% SDS-polyacrylamide gels (Bio-rad ...
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bioRxiv - Synthetic Biology 2022Quote: Protein concentrations of the cell lysates were determined using a Bio-Rad Protein Assay Dye Reagent (Bio-Rad, 5000006). Volumes of lysates containing equal amounts of total protein were boiled at 100°C for 5 minutes after addition of 1/4 volume of 4x SDS-PAGE Loading Solution (250 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein concentrations were determined using the Bradford method38 (Bio-Rad Protein Assay, Bio-Rad Lab, Mississauga, ON, Canada) and immunoblotting was performed as described previously39 ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration for both cytosolic fraction and mitochondrial fraction was then determined by DC Protein Assay (Bio-Rad), and 175 μL of 100–500 μg/ml protein solution was generated with assay buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: The concentrations of protein in the supernatant were determined by using the Bio-Rad protein assay reagent (Bio-Rad). Samples ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using a Bio-Rad DC™ protein assay kit (Bio-Rad, Marnes-La-Coquette, France). Proteins (30 µg ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations in cell-free extracts and purified protein preparations were quantified using Bradford reagent (catalog # 5000006, Bio-Rad), with ovalbumin as a standard ...
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bioRxiv - Physiology 2020Quote: ... Proteins were separated on an Any kD™ Criterion™ TGX Stain-Free™ Protein Gel (Biorad, Hercules, CA) and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: ... 25 µg of total protein was separated on 4-20% Criterion TGX precast midi protein gels (Bio-Rad #5671094) and transferred onto nitrocellulose membranes (Thermo Fisher Scientific #IB23001 ...
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bioRxiv - Immunology 2022Quote: ... Protein concentration in cell lysates was determined by using a standard Coomassie Bradford protein assay (Bio-Rad, Hercules, CA). For SDS-PAGE cell lysates were mixed with an equal volume of 2X SDS sample buffer (50 mMTris ...
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bioRxiv - Cancer Biology 2022Quote: ... was used to determine protein concentration then equal amounts of total proteins were separated by SDS-PAGE (Bio-Rad) and transferred to PVDF membrane (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... The concentration of solubilized protein was determined with a Bio-Rad Protein Assay kit (Bio-Rad, Hercules, CA USA). The retinal lysate with loading buffer was boiled for 3 min prior to loading onto an 8% sodium dodecyl sulfate (SDS)-polyacrylamide gel with the same amount of protein (5ug ...
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bioRxiv - Genomics 2019Quote: ... 20 µg of protein for each sample was loaded onto 7.5% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad) and run at 100V for 60 minutes using 1x Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... The amount of protein in the supernatants was determined by using the Bio-Rad protein assay (Bio-Rad, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Physiology 2019Quote: ... sonicated for 20 seconds and protein concentration was determined with the commercial kit (DC™ Protein Assay; Bio-Rad). Fifteen µg of proteins were reduced with modified Laemmli buffer (40 mM Tris pH 6.8 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Protein concentrations in the resuspended samples were quantified using a DC Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA). After quantification ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Cells were lysed and protein concentration was determined using the DC™ Protein Assay from Biorad (Hercules, Ca, USA) for data normalization.
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bioRxiv - Evolutionary Biology 2020Quote: Equal amounts of protein were run on 4 – 15% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad) followed by blotting to a Polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein samples were resolved using 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad) and transferred onto PVDF membrane (GE Healthcare ...
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bioRxiv - Systems Biology 2019Quote: ... Protein concentration values for each resulting lysate were determined using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) employing vendor provided protocols ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of all fractions was quantified using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad Laboratories ...
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bioRxiv - Pathology 2021Quote: ... The concentration of proteins was determined using a Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, #5000006). A total of 15 μg of protein from each sample were mixed with NuPAGE™ LDS Sample Buffer (Thermofisher ...
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bioRxiv - Developmental Biology 2019Quote: ... Lysates were cleared at 16,000 g and protein concentration determined using the Bio-Rad Protein Assay Dye (Bio-Rad). Lysates were resolved by 8% SDS-PAGE ...
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bioRxiv - Pathology 2019Quote: ... The resulting band sizes were resolved using Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standard (1610375, Bio-Rad). For autoantibody detection ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... The protein lysate concentration was quantified using Pierce BCA Protein Assay Kit (ThermoScientific) and10μg of soluble protein lysates were separated on a 4-12% Bis-Tris gel (BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... 15 min) and the protein amount was quantified with the use of Bio-Rad Protein Assay (Bio-Rad, 5000006). For western blotting the following primary antibodies were used ...
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bioRxiv - Cell Biology 2021Quote: ... Twenty micrograms of protein were separated via electrophoresis using 4 – 20% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad). Proteins were transferred to PVDF membranes and blocked in TBS with 0.1% Tween-20 (TBST ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration of lysates was measured using the BIO-RAD DC protein assay according to the manufacturer’s instructions (Biorad). For precipitation experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein lysates were separated on 12% or 4%-15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad), transferred on a nitrocellulose membrane (BioRad ...
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bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... Protein gels used for protein normalization and blots were imaged using the ChemiDoc™ MP Imaging System (BioRad, 12003154). Antibody information is available in Methods - Key resources table.
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bioRxiv - Immunology 2020Quote: ... The total amount of proteins in lysates was quantified by the DC™ Protein Assay (500-0116JA; Bio-Rad) and 2104 EnVision Multilabel Plate Readers (PerkinElmer ...