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Citations for Bio-Rad :
1801 - 1850 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Total protein concentration of each cell lysate sample was determined by Bradford assay with Protein Assay Dye (Bio-Rad) on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2021Quote: ... and total cellular protein content was determined using the DC Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA). Then ...
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bioRxiv - Genomics 2021Quote: ... Protein concentrations of the lysates were determined using a Bio-Rad DC protein assay kit (Bio-Rad, catalog # 5000111), the lysates were denatured in the SDS-PAGE loading buffer at 100°C for 10 min ...
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bioRxiv - Bioengineering 2020Quote: Protein concentration from each secretome was estimated using the Bio-Rad protein assay reagent (Bio-Rad, Hercules, CA, USA) and bovine serum albumin as standard ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were sonicated on ice for 30 seconds and quantified using a DC™ Protein Assay (Bio-Rad, 5000113) before loading 10 μg of protein to 6-10% polyacrylamide gels ...
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bioRxiv - Immunology 2022Quote: ... The protein samples were separated on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad cat. # 4561086) and transferred to a Hybond Amersham PVDF transfer membrane (MilliporeSigma cat ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants were used for protein quantification via the use of the DC(tm) Protein Assay Kit (Bio-Rad). Equal loading of proteins (10–20 µg ...
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bioRxiv - Microbiology 2022Quote: ... Equal total protein was loaded onto on a Criterion™ XT 4-12% Bis-Tris protein gel (Bio-Rad) and the was run for 2 hours at 100V ...
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bioRxiv - Neuroscience 2023Quote: ... membrane suspension was thawed and protein content determined using a Bradford protein assay (Bio-Rad Laboratories, Inc., Hercules, CA), with bovine albumin (Sigma-Aldrich Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were run on 4-20% precast polyacrylamide gel (4-20% Mini-PROTEAN TGX Precast Protein Gels, BioRad) to separate them and transferred onto PVDF membranes using semi-dry transfer ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was determined using the Bradford colorimetric assay (Quick Start™ Bradford Protein Assay Kit; Bio-Rad; 5000201).
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bioRxiv - Molecular Biology 2022Quote: ... and a calibration curve of protein content was created from the BSA protein standard kit (Bio-Rad Laboratories, CA). An equal amount of protein (50 ng ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were passed through a 26G syringe and protein was quantified by DC Protein Assay (Bio-Rad, cat #5000112). Lysates were diluted to equal volumes and 30 ug protein was loaded per well using the Novex WedgeWell Tris-Glycine Welcome Pack (ThermoFisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... The total protein content was determined using the colorimetric Bio-Rad Protein Assay Kit II (Bio-Rad Laboratories, CA). Seven dilutions of a protein standard containing 0 to 30 µg/mL of the total protein content were used ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the samples was determined by the BioRad Protein Assay method (BioRad Laboratories, Hercules, CA, USA). For our gels ...
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bioRxiv - Molecular Biology 2024Quote: ... 10µg of total protein was loaded per lane onto a 4-20% Criterion Tris-HCl Protein gel (Bio-Rad) and separated by electrophoresis at 150 V for 1hr ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µg total protein was loaded on AnykD™ Criterion™ TGX™ Precast Midi Protein Gel (Biorad, #5671125) and 60 kDa region was excised from the Coomassie stained gel ...
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bioRxiv - Biochemistry 2023Quote: ... The protein concentration of each lysate was analyzed using Bio-Rad protein assay reagent according to the manufacturer’s instructions (Biorad). Luciferase units obtained were normalized to the protein content of each well ...
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bioRxiv - Biophysics 2024Quote: ... Total protein concentration in purified microsomes and RyR1 fractions was measured with Quick Start Bradford Protein Assay (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Total protein concentration was determined by Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 20µg of protein was loaded into a 4-15% gradient mini-PROTEAN TGX precast protein gel (4561086, Bio-Rad). Gels were run at 150V in 1x Tris/Glycine/SDS buffer (1610772 ...
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bioRxiv - Microbiology 2023Quote: ... both proteins were supplemented with 5 mM MgCl2 and protein concentration was determined using a colorimetric assay (Bio-Rad). Proteins were then mixed at a 2.5:1 mass ratio of KtrC to N-terminal Strep-tagged KtrA (11 mg of KtrC and 4.5 mg of N-terminal Strep-tagged KtrA in 10 mL total volume of 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified by centrifugation at 16,000 × g for 10 min and the protein concentration was determined using a Lowry assay (DC Protein Assay, BioRAD). Nucleic acid coated beads were incubated with 2 mg protein of cell lysates for 60 min and then washed three times with TAP buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Elution of biotinylated protein was performed by heating the beads in 30LμL of 2X protein loading buffer (1610737; BioRad) supplemented with 2LmM biotin + 20LmM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford colorimetric assay (Quick Start™ Bradford Protein Assay Kit; Bio-Rad; 5000201).
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT) until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Proteins were eluted with 10 ml elution buffer (50mM Tris-HCl (PH=8),150mM NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were separated by SDS-PAGE and fluorescent proteins were visualized using the ChemiDocTM MP Imaging system (Bio-Rad). The protein gel was then stained in Coomassie blue solution.
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations were determined by Bradford Assay with the Bio-Rad Protein Assay Dye reagent concentrate (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... protein was separated by SDS-PAGE on a 4-15% Criterion TGX pre-cast midi protein gel (Bio-Rad), and transferred to nitrocellulose membrane using a standard tank electrotransfer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Identical amounts of proteins (20-40μg) were electrophoresed on 4–15% Mini-PROTEAN TGX precast protein gels (Bio-Rad). For transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein concentrations of sample supernatants were measured with the Bio-Rad protein assay dye reagent (Bio-Rad, 5000006). Equivalent amounts of protein samples were mixed with Laemmli buffer (100 mM Tris-HCl pH 6.8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Identical amounts of proteins (20-40μg) were electrophoresed on 4–15% Mini-PROTEAN TGX precast protein gels (Bio-Rad). For transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... The total protein concentration was determined using Bradford protein assay according to the instructions from the manufacturer (Bio-Rad). POSTN protein expression from the ELISA assay was normalized with the total protein levels ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (30–50 μg) were loaded onto 4%–20% Mini-PROTEAN TGX Precast Protein Gels (BioRad). Proteins were separated by electrophoresis at 16 mA followed by transferring the proteins to Immobilon-P membranes (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad). Proteins were transferred to the PVDF membrane using a Trans-blotter (Biorad) ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration das measured of each eluate sample in a 1:20 dilution by Bradford (BioRad protein assay). Per sample ...
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bioRxiv - Neuroscience 2024Quote: Protein samples from mouse tissue were prepared by diluting 20-40 μg of protein with 1X Laemmli buffer (Biorad) supplemented with beta-mercaptoethanol (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supernatant was recover and protein concentration was measured using the DC™ Protein Assay Kit II (Bio-Rad, 5000112). Lysates were boiled in the NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated by electrophoresis using 4-12% Bis-Tris mini protein gels and transferred to PVDF membranes (BioRad). Blots were incubated with primary antibodies at 4°C and then with HRP conjugated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 1 tablet EDTA-free protease inhibitors) and protein content was determined using a Bradford protein assay (Bio-Rad, #5000001). 50-100 µg protein was run on an SDS-PAGE and blotted using a wet-transfer system (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... Total protein concentration was determined by a detergent compatible DC™ Protein Assay Kit II (Bio-Rad Laboratories, #5000112). For Western blot analysis ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The protein concentration in the thylakoid membrane fraction was determined via Bradford’s method (Protein Assay, Bio-Rad, Hercules, CA) using a bovine serum albumin solution of known concentration as a standard.
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bioRxiv - Neuroscience 2024Quote: The total amount of protein present in the samples was quantified using the Bio-Rad Protein assay (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... The resin was resuspended in 5 mL of extraction buffer and transferred to a gravity flow column (Biorad, Hercules, CA). After loading ...
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bioRxiv - Molecular Biology 2019Quote: ... Amplicons were then run on a 1.5% agarose gel at 130 V for 40 min and the DNA in the 235bp band extracted using Freeze’N Squeeze DNA Gel Extraction Spin Columns (BioRad 7326165). Extracted DNA was sequenced on an Illumina NextSeq500 using SV40_NLS_Read1 ...
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bioRxiv - Biochemistry 2021Quote: ... AA-tRNAIles were purified by phenol/chloroform extraction followed by two consecutive steps on Bio-Spin P30 columns (Bio-Rad) and dialyzed against 10 mM NaOAc pH 4.5 ...
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bioRxiv - Immunology 2021Quote: ... Immunoprecipitated and input DNA were extracted by phenol-chloroform extraction and used for qPCR with SsoAdvanced Universal SYBR Green supermix (Biorad). All primer sequences used are listed in Supplementary Table 5.
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bioRxiv - Plant Biology 2022Quote: ... The resin was resuspended in 5 mL of extraction buffer and transferred to a gravity flow column (Biorad, Hercules, CA). After loading ...
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bioRxiv - Molecular Biology 2024Quote: 5 μL of the final extraction elutions or the resuspended pellet were blotted onto a nitrocellulose membrane (0.2 μm; Bio-Rad). The transthyretin from the extractions was observed by using anti-transthyretin antibody (1:1000 ...