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Citations for Bio-Rad :
1851 - 1900 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then sonicated and their protein content was quantified using the Bradford protein assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufactureŕs instruction and measured using the EonTM High Performance Microplate Spectrophotometer (BioTek ...
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bioRxiv - Cell Biology 2022Quote: ... 30 µg of total protein per sample was loaded on NuPage 4-12% Bis-Tris Protein gels (Thermo Fischer Scientific) or 4–15% Mini-PROTEAN TGX StainFree Protein gels (Biorad) and run at 200 V as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Each gel was loaded with a Precision Plus Protein™ Dual Color Standards as a protein marker (Bio-Rad, 1610374). Gel electrophoresis was stopped when the Bromophenol blue dye reached the bottom of the gel ...
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bioRxiv - Physiology 2020Quote: ... Protein concentration was determined in triplicate with a commercial colorimetric assay (Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia), against bovine serum albumin standards (BSA ...
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bioRxiv - Genomics 2019Quote: ... media were collected for insulin determination and rat islet cells were lysed with acid-ethanol (0.2 mM HCl in 75% ethanol) to extract total insulin content or with protein lysis buffer to measure total protein content (Bradford, BioRad). The amount of insulin released in the medium and remaining in the cells was measured by insulin Elisa kit (Mercodia) ...
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bioRxiv - Cancer Biology 2019Quote: ... The volume of the target protein bands and total protein volume of each lane were normalized and quantified using ImageLab software (Biorad). For all figures shown ...
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bioRxiv - Plant Biology 2019Quote: ... The amount of protein recovered after lysis was determined using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad), with bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Trehalase activity was standardized to total protein content using the DC protein quantification technique according to the manufacturer’s recommendations (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Equal protein content for each condition (targeting 10 μg) was run on 10% Mini-PROTEAN TGX precast protein gels (Biorad). After electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... The quantification of protein content in exosome suspensions and whole-cell lysates was undertaken by a modified Lowry assay (DC Protein Assay, BIORAD). For immunoblotting ...
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bioRxiv - Physiology 2021Quote: Equal amounts of skeletal muscle protein extract (45µg) were separated on Mini-Protean TGX precast protein gels (Biorad, CA, USA) and transferred to a PVDF membrane using a Trans-Blot semi-dry transfer system (Biorad ...
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bioRxiv - Biochemistry 2021Quote: ... Equal protein content for each condition (targeting 10 μg) was run on 10% Mini-PROTEAN TGX precast protein gels (Biorad). After electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... AS and EVs were lysed in RIPA buffer and protein concentration was measured with DC Protein Assay (Biorad, 500-0116), using BSA as standard ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatants were collected and protein concentration was determined using the Bradford-based Bio-Rad protein assay (Bio-Rad). Samples were then denatured and separated using SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were eluted in SDS page loading buffer and separated on 4-15% Mini-PROTEAN TGXTM Precast Protein Gels (BioRad). Samples were processed for immunoblotting or fixed and stained for mass spectrometry using Imperial protein stain (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... The amounts of isolated mitochondria were expressed as the protein concentration using a Bio-Rad protein assay kit (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... for 20 min at 4 °C) protein concentrations in supernatants collected were determined using a Bradford protein assay (Bio-Rad). Remaining supernatants were mixed with sample buffer at 3:1 v /v ratio ...
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bioRxiv - Genetics 2022Quote: ... Normalization among different technical replicates and conditions was carried out by normalizing the relative luminescence units (RLUs) against total protein content (calculated with the Bradford protein assay, BioRad). Each condition was measured in quadruplicates and each experiment was performed at least twice.
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bioRxiv - Biochemistry 2022Quote: ... The protein content of the LDL sample was determined by a protein assay kit (Bio-Rad Laboratories, Inc., Tokyo, Japan) based on the Bradford method using bovine serum albumin as a standard ...
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bioRxiv - Biochemistry 2022Quote: ... protein samples were separated by precast 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad Laboratories 5678085), transferred to a nitrocellulose membrane using Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs (Bio-Rad Laboratories 1704159EDU ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... 1x PIC) using a sonifier BRANSON 250.The protein in crude homogenates was determined by Bradford protein assay (BioRad, USA) and 50 μg of protein was used for a subsequent chloroform/methanol extraction ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed and normalised for protein concentration using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Inc. # 5000001) and a spectrophotometer ...
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bioRxiv - Molecular Biology 2020Quote: ... Soluble extract was collected and total protein concentration determined using Protein Assay Dye Reagent Concentrate (BIO-RAD, Cat No 5000006) with BSA as standard ...
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was dissolved in RapiGest SF (Waters) and the protein concentration was determined (Bio-Rad protein assay, Bio-Rad). The mitoribosomal lysates were frozen in liquid nitrogen and analyzed at the Proteomics core facility of the Medical University of Vienna ...
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was dissolved in RapiGest SF (Waters) and the protein concentration was determined (Bio-Rad protein assay, Bio-Rad). The mitoribosomal lysates were frozen in liquid nitrogen and analyzed at the Proteomics core facility of the Medical University of Vienna ...
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bioRxiv - Cancer Biology 2021Quote: ... centrifuged for 20 minutes at 13,000 rpm at 4°C and the protein concentration in the supernatant was measured with an DC protein assay (BioRad). Samples were separated by SDS-PAGE ...
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bioRxiv - Pathology 2022Quote: ... The soluble protein extracts were separated by SDS-PAGE (4-15% mini-protein TGX precast gel, 12 or 15 well, BioRad) and transferred to a PVDF membrane (Trans-blot Turbo mini-Format 0.2 μM PVDF ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Equal amounts of protein were run on 4 – 15% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad, USA) followed by blotting to a Polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: All proteins were snap frozen in single-use aliquots and protein purity was assessed by 10% SDS-PAGE (BioRad Laboratories) and stained with Coomassie Brilliant Blue (Supplemental Figure S1).
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bioRxiv - Cancer Biology 2019Quote: ... Quantification of protein concentration was done using the RC DC Protein Assay (Bio-Rad, Hercules, CA; Cat. No. 500–0119). Equal amounts of lysate were denatured and loaded onto a 10% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein band of interest was subsequently quantitated (normalized to the reference protein band) using quantity one software (Bio-rad). Antibodies used for western blot analysis are listed in Suppl ...
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bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
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bioRxiv - Physiology 2021Quote: ... the concentration of protein in the whole homogenates was determined with the DC protein assay kit (Bio-Rad, Hercules, CA). Equal amounts of protein from each sample were dissolved in Laemmli buffer ...
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bioRxiv - Physiology 2020Quote: ... Ten micrograms of protein were subjected to SDS-PAGE on 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad) and transferred to polyvinylidene diflouride (PVDF ...
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bioRxiv - Physiology 2020Quote: ... equal amounts of protein (20 ug) were loaded in 4-12% - Criterion XT Bis-Tris protein gels (Bio-Rad, CA) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: After the protein concentration of cell lysates and homogenates had been determined using the DC Protein Assay Kit (Bio-Rad) 20 µg of each sample were dissolved in NuPAGE LDS Sample buffer (including 2% β-mercaptoethanol) ...
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bioRxiv - Immunology 2021Quote: ... Total protein content was measured by the Pierce BCA Protein Assay Kit (Thermo) and equivalent protein levels were loaded into an polyacrylamide gel (BioRad). Samples were transferred onto a nitrocellulose membrane using a semi-dry transfer apparatus before blocking with a 5% skim milk solution for thirty minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The amount of protein in each tissue sample was quantified using detergent compatible protein assay (Bio-Rad, Hercules, CA, USA). Then ...
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bioRxiv - Physiology 2019Quote: ... Lysates were separated from beads by pipet and an aliquot was preserved for protein quantification (Bio-Rad, DC Protein Assay). Lysates were then washed of lipids and pigments in 200µL petroleum ether (40-60°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Clarified protein lysates were measured according to standard methods using a DC protein assay kit (Bio-rad, Mississauga, Ontario, Canada). Bradford assays (Bio-Rad ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were clarified by centrifugation at 6,000 rpm for 7 min and protein concentration was determined using a DC protein assay kit (Bio-Rad). Samples containing equal amounts of protein were brought to a total volume of 1 ml with appropriate buffer.
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were loaded on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-rad, Hercules, CA) and transferred onto PVDF membranes (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... precast protein gels in sodium dodecyl sulfate (SDS) buffer followed by electrophoretic transfer of proteins to nitrocellulose membrane (BioRad, #2895). After blocking in 5% nonfat dried milk diluted in tris-buffered saline and 1% tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μg of protein was loaded on 4-20% Mini-PROTEAN TGS Precast Protein Gels (Cat. No. 4561094, Bio-Rad) from each condition and ran at 100 V for 1 hour followed by transferring the protein on a PVDF membrane ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were then loaded (15-20 μg of protein per well) in a 4–20% Precast Protein Gel (Biorad) and resolved by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared for 10 min at 14000 rpm at 4°C and protein concentration of the supernatant was determined by using Protein Assay (BioRad). The co-immunoprecipitation was performed using GFP trap® beads (Chromotek ...
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bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Cat. 5000006).
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bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Cat. 5000006).