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Citations for Bio-Rad :
1901 - 1950 of 2583 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally boiled for five min at 95ºC in Laemmli sample buffer (Bio-Rad, Hercules, CA, USA) supplemented with 100mM DTT.
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bioRxiv - Genetics 2023Quote: ... 1 ml 2xYNB with 2% glucose was added to the sorted cells in sheath fluid (PBS, BioRad #12012932) and they were transferred to a shaking incubator and grown at 30 °C ...
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bioRxiv - Immunology 2023Quote: ... We analyzed cells on the same day using a Bio-Rad Ze5 Analyzer (Bio-Rad, Hercules, CA, USA). Single color controls enabled compensation and spectral unmixing of samples ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lysates were separated on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, USA) and transferred to a nitrocellulose membrane (Cell Signalling Technology) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µl was transferred to each chamber in a cell counting slide with dual chambers (BioRad, #145-0011)) ...
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresis was performed at a constant current of 150 mV in a Mini-PROTEAN Tetra cell (BIO-RAD). Proteins were then transferred onto nitrocellulose membrane (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... resuspended in cell suspension buffer and embedded in agarose before they were transferred into plug molds (Biorad; #1703591). After a 30min incubation at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... After taking a sample from the cell-free extract (CFE) the chromatography column (BioRad, Poly-Prep Chromatography Column) was prepared by adding 1 ml of resin (His-select nickel affinity gel) ...
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bioRxiv - Plant Biology 2024Quote: ... it was placed into a Mini-Sub® Cell gel tank (Bio-Rad Laboratories Ltd., Hemel Hempstead, UK) and submerged in 0.5x TBE buffer ...
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bioRxiv - Immunology 2023Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer or directly into 2X XT Sample buffer (Bio-Rad #1610791) + 1X XT Reducing Agent (Bio-Rad #1610792 ...
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bioRxiv - Physiology 2023Quote: ... cell or tissue lysates were resolved by SDS-PAGE and transferred to nitrocellulose membrane (Bio-Rad Laboratories, USA). Blots were blocked with 5% nonfat milk in phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2024Quote: ... The cell/plasmid mixture was transferred to a chilled Gene Pulser 0.1 cm Cuvette (Bio-Rad 165-2089) and electroporated in a Gene Pulser Xcell (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: The cell proliferation was evaluated after 24 hours of transfection using the AlamarBlue proliferation assay (BioRad Laboratories, USA). AlamarBlue was added at 1:10 ratio to the cells and quadruplicates of each condition was measured every hour on the infinite M200 fluorescence plate reader set to 37°C (Tecan Systems ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cells was extracted using the Aurum™ Total RNA Mini Kit (Bio-Rad, CA, USA), and 100ng of RNA was used with iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: Normalized lysate samples were resolved with 12% SDS-PAGE gels using Mini-PROTEAN® Tetra Cell (Bio-Rad) and blotted to poly(vinylidene difluoride ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants of cell lysates were passed through a nickel bead column (Profinity IMAC Ni-Charged Resin, Bio-Rad) to allow binding of lm_ApbE-6xHis ...
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bioRxiv - Cell Biology 2024Quote: Cells were washed in Dulbecco’s phosphate buffered saline (DPBS) and boiled in 1x Laemmli sample buffer (Bio-rad). Samples were separated on a 10% acrylamide gel and transferred to nitrocellulose membrane ...
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bioRxiv - Microbiology 2020Quote: ... or blotted onto nitrocellulose or PVDF membranes using a Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad) at 15V for 15 min ...
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bioRxiv - Biophysics 2021Quote: ... Tetranucleosome template was purified from the backbone by preparative acrylamide electrophoresis using a Model 491 Prep Cell (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... 20 μg of protein for each cell lysate was run on a 4-15% SDS gel (Bio-Rad – 4561083DC) and electrotransferred onto a PVDF membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Western blotting was performed in 20% ethanol/MOPS-SDS buffer in a Mini Trans-Blot electrophoretic cell (Bio-Rad) using Immobilon-FL PVDF membrane (Merck Millipore ...
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bioRxiv - Developmental Biology 2020Quote: ... These cell extracts were run on a 10% acrylamide gel before being transferred to a PVDF membrane (Bio-Rad). The membranes were blocked in 5% milk prior to incubation with primary antibodies overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were electroporated in 2-mm cuvettes (600 V, 50 μF, 200 Ω) by using Gene Pulser Xcell (Biorad). Immediately after electroporation ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 μl aliquots of the resulting competent cells were electroporated using a Gene Pulser Xcell Electroporation System (Bio-Rad) with 50 – 200 ng plasmid with the following settings ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from infected cells at various times after infection using an Aurum total RNA minikit (Bio-Rad). For RT-qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were quantified for protein concentrations using the Bio-Rad DC™ Protein Assay kit (Cat.5000112, BioRad) and loaded to a 10% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in TE buffer and electroporated into cells using an Eppendorf Electroporator 2510 in 1mm electroporation cuvettes (Bio-Rad).
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bioRxiv - Pathology 2019Quote: ... by SDS-PAGE at 100 V for 2 hours in a Mini-PROTEAN® Tetra cell apparatus (Bio-Rad). The Kaleidoscope commercial standard Precision Plus Protein Standard (Bio-Rad ...
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bioRxiv - Systems Biology 2020Quote: Whole cell fractions were isolated using RIPA buffer and protein concentrations were determined using a Bradford Assay (Bio-Rad). 20µg whole cell lysate were separated on 8-12% SDS-PAGE gels (depending on the molecular weight of the target proteins) ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in approximately 10 μl M9 medium and spotted onto 1% low-fluorescence agarose pads (Bio-Rad) prepared in M9 medium and covered with a no1.5 glass coverslip ...
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bioRxiv - Cell Biology 2019Quote: ... The cell distribution and morphology were analyzed by SEM and confocal laser scanning microscopy (CLSM) (Bio-Rad MRC 600).
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bioRxiv - Genetics 2021Quote: ... Fifty microliters of cells were added to a chilled Gene Pulser cuvette with 0.2 cm electrode gap (Bio-Rad) and subjected to a pulse of 1.5 kV ...
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bioRxiv - Immunology 2021Quote: ... yeast (24 h post-induction) were counted using the TC20™ automated cell counter (Bio-Rad Hercules, CA, USA). Cells were centrifuged at 4,000 × G for 10 min and resuspended in PBS (Thermofisher) ...
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bioRxiv - Bioengineering 2021Quote: Equivalent number of T cells (1×10e6) were lysed in Laemmli Sample Buffer with β-mercaptoethanol (Bio-Rad, CA). Total cell lysate for each sample were resolved on 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were then used to isolate the total RNA using the Aurum Total RNA Mini Kit (Biorad, #7326820) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Then the cells and DNA mixture were transferred to cuvette and inserted into Gene Pulser Xcell Electroporation System (Biorad) using following settings:
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates and sEV/exosome samples (derived from 1.6×107 cells) were subjected to 12 % SDS-PAGE and Western blotting (Biorad) or to 4-12 % (Bis-Tris gel ...
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Functional characterization of a DNA-dependent AAA ATPase in SimranZ1, a F cluster mycobacteriophagebioRxiv - Biochemistry 2021Quote: ... The protein was then transferred to a PVDF membrane by using Mini-PROTEAN Tetra Cell (Bio-Rad, United States). The membrane was blocked overnight by using 5% BSA at 4 °C followed by incubation with mouse anti-His6 antiserum (Santacruz Biotech ...
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bioRxiv - Plant Biology 2021Quote: ... Particle bombardment of onion peel cells was performed using biolistics particle delivery system PDS-1000/He (Bio-Rad, USA) as per the protocol of Lee et al ...
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bioRxiv - Plant Biology 2020Quote: Barley epidermal cells were transiently transformed by biolistic particle bombardment using the PDS-1000/HE (Biorad, Hercules, CA; USA). For this 7d old primary leaves of barley were cut and placed on 0.8% water-agar ...
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bioRxiv - Microbiology 2020Quote: ... an equal number of transduced and puromycin-selected HeLa cells or PBMCs were lysed with RIPA buffer (Bio-Rad), mixed with sample buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Debris was inspected on a brightfield microscope and nuclei were counted on an TC20 automated cell counter (Bio-Rad) after the addition of Trypan Blue.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein concentrations in the cell lysates (mg/mL) were assayed using the Bradford protein assay (Bio-Rad, Hercules, CA) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then transferred at room temperature (RT) onto a nitrocellulose membrane using a semi-dry transfer cell (Biorad) (20 V ...
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bioRxiv - Microbiology 2020Quote: ... cells were trypsinized and 2.0×104 cells were resuspended and lysed per 10µL 1x Laemmli Sample Buffer (161-0747, Bio-Rad). 10µL cell lysate was separated by SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of the epithelial cell proteins in the resulting supernatant was determined using the Bradford method (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates and lipid raft protein isolates were prepared by sonication in 4x Laemmli sample buffer (Bio-Rad, 1610747) and then loaded into 10% SDS-polyacrylamide gels for electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... signals were obtained with peroxidase-conjugated secondary antibodies (Cell Signalling Technology) and revealed with Clarity Western ECL kit (Biorad) and a ChemiDoc ™ XRS Imaging system (BioRad).
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bioRxiv - Synthetic Biology 2022Quote: Protein concentrations of the cell lysates were determined using a Bio-Rad Protein Assay Dye Reagent (Bio-Rad, 5000006). Volumes of lysates containing equal amounts of total protein were boiled at 100°C for 5 minutes after addition of 1/4 volume of 4x SDS-PAGE Loading Solution (250 mM Tris ...