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Citations for Bio-Rad :
1751 - 1800 of 2723 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... at 120 volts for 1.5 h using Criterion™ vertical electrophoresis cell (BioRad, Cat# 1656001, USA). The resolved proteins were transferred from the gel to Immobilon®-P PVDF membranes (Millipore Sigma ...
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bioRxiv - Cell Biology 2024Quote: Purified RNAs from cells and tissues were mixed with iScript Reverse Transcription Supermix (Bio-Rad, 1708841) and reverse transcription was performed according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... Total proteins (20 μg) of whole cell extracts were resuspended in 1x LAemmli Buffer (BioRad #1610747), boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations for the whole cell extracts were determined using a Bradford protein assay (Bio-Rad). The extracts were aliquoted ...
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bioRxiv - Microbiology 2024Quote: ... 0.45μm (ThermoFischer # 88585) using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad #1703940). The membrane is then saturated in 5% BSA (GoldBio #A-420 ...
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bioRxiv - Cancer Biology 2021Quote: RNA was isolated from melanoma cells using the Aurum Total RNA Mini Kit (Bio-Rad Cat# 7326820) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were then resuspended in PBS and 2X Laemmli Sample Buffer (Bio-Rad, Cat. No. 1610737) containing 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatants as whole-cell lysates (WCLs) were quantified using a DC Protein Assay kit (Bio-Rad). Then ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins in the gel were then transferred to a nitrocellulose membrane in wet trans-blot cell (Biorad). The membranes were blocked in blocking buffer (5% Nonfat Dried Milk in PBS + 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed in cold PBS and lysed in 2x Laemmli Sample Buffer (Bio-Rad Laboratories, 1,610,737) supplemented with DDT ...
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bioRxiv - Immunology 2021Quote: ... Total cell lysate was extracted using RIPA buffer and quantified using the DC protein assay (Bio-Rad). 15–30 μg of protein was loaded and run on 4%–12% SDS PAGE gels ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD) at 20 V for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were incubated with APC (Lynx Rapid APC antibody conjugation kit, Cat # LNK031APC, Bio-Rad Laboratories, Inc.)-conjugated cytokeratin 6 (CK6 ...
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bioRxiv - Physiology 2022Quote: ... and viability confirmed to be >85% using a TC20™ Automated Cell Counter (BioRad, Cat. No. 1450102).
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bioRxiv - Cell Biology 2022Quote: Unstained SDS gels were transferred to a nitrocellulose membrane using a semi-dry transfer cell (Bio-rad) for 2 hours at 12 volts ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then transferred onto nitrocellulose (NC) membrane using an electrophoretic transfer cell (Bio-Rad Laboratories, USA) at RT (28) ...
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bioRxiv - Genomics 2020Quote: ... Cells were embedded in thin low-melting-point agarose plugs (CHEF Genomic DNA Plug Kit, Bio-Rad). Subsequent handling of the DNA followed protocols from Bionano Genomics using the Bionano Prep Blood and Cell Culture DNA Isolation Kit ...
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bioRxiv - Genetics 2019Quote: ... and transferred to nitrocellulose membranes using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were blocked in 5% non-fat milk and incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... 1D separation was run according to the O’Farrell protocol63 (adapted for Bio-Rad Mini-PROTEAN Tube Cell). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... 2D separation was run according to the Laemmli protocol64 (adapted for Bio-Rad Mini-PROTEAN Tetra Cell). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were transferred onto a nitrocellulose membrane utilizing a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad) in Tris-Glycine Buffer (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... mobilis competent cells were prepared as previously described (30) and transformed with plasmids by electroporation (Bio-Rad Gene Pulser ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then pulsed with 1.8 kV in a 1-mm-gap cuvette (Bio-Rad, USA).
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bioRxiv - Pathology 2019Quote: ... the cells were incubated with anti- MNV VP1 rabbit polyclonal antibody labeled by Dylight 488 (LNK221D488, BioRad) for 30 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... and cell lysates were analyzed by SDS-PAGE using 4–20% gradient Tis-HCl gels (BioRad, #3450034). Proteins were transferred onto 0.2-μm nitrocellulose membrane ...
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bioRxiv - Bioengineering 2021Quote: ADMSC metabolic activity was tracked over 14 days using the alamarBlue® Cell Viability Assay (Bio-Rad). Media was removed from samples of S-50 or P-0 (n = 5 each) ...
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bioRxiv - Cancer Biology 2020Quote: Cell extracts were harvested by direct addition of 2x SDS sample buffer (1610747, BioRad Laboratories, Alcobendas, Spain) containing 100 mM DTT to the culture plate and incubation on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 μl of the cells suspension were mixed with 10 μl of Trypan Blue (Bio-Rad, #1450021) and the number of the cells and viability were analysed using BioRad TC20 cell counter ...
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bioRxiv - Physiology 2021Quote: ... and proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 70 volts for 2 hours at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The corresponding constructs were transiently co-transformed in onion epidermis cells using the particle bombardment (Bio-Rad) method as described previously (Lee et al. ...
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bioRxiv - Microbiology 2020Quote: ... cells were pelleted and lysed directly in 1x Laemmli sample buffer with fresh ß-mercaptoethanol (Bio-Rad), sonicated to break up DNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM EDTA) using the Mini-PROTEAN Tetra Vertical Electrophoresis cell (gel size 10×8 cm, BioRad). Each run was preceded by a 30 min pre-run at 300 V to warm the gel ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from equal number of cells were separated on TGX Stain-Free SDS-PAGE gel (Bio-Rad), transferred to polyvinylidene difluoride membrnaes (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... The gels were then blotted using the wet-transfer Trans-Blot® Cell system from Bio-Rad. Proteins were transferred to Hybond-P polyvinylidene difluoride membranes (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin fractions or cell lysates were separated on 4-15% Mini-PROTEAN TGXTM Precast Protein Gels (BioRad). Proteins were transferred onto PVDF membranes (0.45µm ...
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bioRxiv - Biophysics 2021Quote: Cells were mounted on a 2% agarose pad containing resuspension medium using a gene frame (Bio-Rad). Cells were concentrated by centrifugation (3300g for 30 s ...
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bioRxiv - Cell Biology 2021Quote: ... 20% methanol) for 15 minutes before assembly in the Mini Trans-Blot transfer cell (Bio-Rad, 1703935). The transfer was done for 16 h at 4°C and at 110 mA constant current ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatant was discarded and the RFP+ cells were frozen (−80°C) in PureZol (Bio-Rad. #732-6890). Triplicates of RNA from RFP+ cells were purified as described above.
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bioRxiv - Molecular Biology 2022Quote: ... 1 × 107 cells were electroporated (Gene Pulser Xcell System, Bio-Rad: 250 V, 500 mF, 0.4cm cuvettes) with the following plasmid vectors ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates from 3×106 cells were separated on 4-20 % gradient Tris-glycine polyacrylamide gels (BioRad 4568094), electroblotted onto a PVDF membrane (Pierce 88518) ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid DNA was purified by agarose gel electrophoresis using the Model 491 Prep Cell (Bio-Rad). All DNA concentrations are expressed in moles of molecules.
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed in RIPA buffer and protein concentrations were determined using Bradford reagent (Bio-Rad Laboratories). Equal amounts of protein were applied to SDS-PAGE gels which were run at 150 V initially and 200 V thereafter ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein concentrations of cell lysates were measured with the Protein DC assay (Bio-Rad, 500-0111). Phosphorylation of histone H3 and Aurora kinases A ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice and resuspended in wash buffer for data acquisition at ZE5 cytometer (Biorad).
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were separated with 12% precast polyacrylamide gels and Tris/Glycine/SDS running buffer (Bio-Rad). Following protein transfer ...
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bioRxiv - Immunology 2020Quote: ... The fixed and permeabilized cell monolayer was stained with anti-nucleoprotein (Clone: AA5H, Bio-Rad Antibodies, UK) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed with TCA buffer and protein concentrations were determined using DC protein assay kit (BioRad). Protein separation was performed with a 7.5% SDS-PAGE and transferred to nitrocellulose at 100 V for 90 mins.
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bioRxiv - Microbiology 2020Quote: ... and transferred to Hybond-N+ membrane (Amersham) by electroblotting using the Trans-blot cell from Bio-Rad in 1x TBE buffer (89 mM Tris-base ...
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bioRxiv - Cell Biology 2020Quote: Cell lysates were run on BioRad 4-10% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA) and transferred to PVDF membranes ...
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bioRxiv - Microbiology 2021Quote: ... 25 mL of log-phase Sodalis cells (OD600= 0.3∼0.5; SmartSpec Plus spectrophotometer; Bio-Rad, Hercules, CA) were washed consecutively in 25 mL ...