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Citations for Bio-Rad :
2151 - 2200 of 2723 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... The embedded cells were then lysed and DNA was separated using a CHEF Mapper® XA System (BioRad, Munich, Germany) essentially as previously described (Swiatek et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...
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bioRxiv - Cancer Biology 2019Quote: ... total RNA was extracted from dissociated single cells using QIAGEN RNease Mini Kit and cDNA was prepared using 1µg RNA with iScript cDNA Synthesis Kit (Bio-Rad). SYBR green-based QPCR was performed using murine primers listed as below (GLI1 ...
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bioRxiv - Immunology 2019Quote: ... Donor CD103+ KLRG1-CD8 T cells from LP of transplanted tissue were identified following the gating strategy in FigureS11 and single cells were sorted into 96-well plates (BioRad) containing 5 µL capture buffer (20 mM Tris-HCl pH8 ...
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Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cellsbioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4– 15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and 25-30 μg of protein were loaded on a 7.5% separating gel using Mini Trans-Blot Cell (Bio-Rad). Nitrocellulose membranes were stained with primary antibodies ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h and then incubated with primary overnight and secondary antibodies for 2 h ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Microbiology 2019Quote: Approximately 1 × 105 bacterial cells were suspended in PBS containing 1% (w/v) PBS-BSA and incubated with fH (Biorad) at a final concentration of 5 μg/mL for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h in nonfat-milk ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 2-10 μl of unmethylated DNA (>50 ng/μl) was added to cells and transferred to a 0.4 cm gap electroporation cuvette (Bio-Rad). Cells were exposed to 2.5kV for 4-6 msec ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in 1X PBS and mechanically lysed by sonication before protein standardized by DC assay (BIO-RAD) and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR) ...
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bioRxiv - Plant Biology 2020Quote: Transient expression in onion cells (bulb sliced to ~16 cm2) was conducted using the Biolistic PDS 1000/He Particle Delivery System (Biorad) (1100 psi ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: ... DGF-298 electrocompetent cells were transformed with 300 ng of the purified PCR product (described above) by electroporation in a Genepulser XCell (Biorad) at 1800 V ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were separated by SDS-PAGE using a Mini-Protein Tera Cell system (Bio-Rad Laboratories, Inc., Hercules, USA, CA). Proteins were visualized by CBB staining as described elsewhere.
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bioRxiv - Biochemistry 2020Quote: ... by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany). Proteins were transferred to a nitrocellulose membrane (Millipore ...
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bioRxiv - Neuroscience 2021Quote: MV pellets were sonicated three times for 10 s at 20 Hz (Vibra cell VCX130) in 2% SDS and heated at 56°C in Laemmli loading buffer (Biorad). The protein content was measured using the Pierce 660 nm protein assay kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The quantification of protein content in exosome suspensions and whole-cell lysates was undertaken by a modified Lowry assay (DC Protein Assay, BIORAD). For immunoblotting ...
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bioRxiv - Cancer Biology 2021Quote: ... based on the manufacturer’s protocol and 30 μg of protein lysate of each sample was separated by electrophoresis in 7.5% or 12% SDS-PAGE gel and transferred into 0.45 μm PVDF transfer membrane using a Semi-Dry transfer cell (Trans-Blot SD, Bio-Rad). After transfer ...
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bioRxiv - Cancer Biology 2019Quote: RNA was isolated from cell lines or flash-frozen tissue as described above was reverse-transcribed using iScript (Bio-Rad). Real-Time PCR was conducted using VeriQuest SYBR Green qPCR Master Mix (Affymetrix) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were separated by 10% SDS-PAGE followed by electrophoretic transfer to polyvinylidene difluoride membranes (Bio-Rad, Hercules CA). Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween 20 and incubated at 4°C overnight with the TRPM4 antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were Western blotted overnight with constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA). Membranes were destained from residual Coomassie traces by washes in methanol for several minutes ...
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bioRxiv - Microbiology 2020Quote: ... The slides were read under a fluorescence microscope and photographs were taken with a ZOE™ fluorescent cell imager (BioRad).
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bioRxiv - Microbiology 2021Quote: ... Lysates were run on 12% polyacrylamide gel and transferred to a nitrocellulose membrane using the Trans-blot SD semi-dry transfer cell (Biorad). To represent the same number of cells in each lane ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were in-gel rehydrated for 16 hrs and isoelectrically focused on 7 cm pH 3-10 IPG strips to 10,000 Vh on a Protean® IEF Cell (BioRad). After focusing ...
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bioRxiv - Microbiology 2019Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad) per the manufacturer’s instructions and blocked with 100 mg/mL skim milk in 1x TBS/Tween 20 ...
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bioRxiv - Cancer Biology 2020Quote: HEL 92.1.7 cells were transfected by electroporation at various conditions using the Gene Pulser Xcell™ Electroporation System (BioRad, USA). In brief ...
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bioRxiv - Cancer Biology 2021Quote: ... RNP complexes were added to 200,000 cells in 200µL electroporation buffer (0.2 cm cuvette) and electroporated via the Gene Pulser Xcell™ Electroporation System (Bio-Rad) using exponential decay pulses (145V ...
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bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
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bioRxiv - Cell Biology 2020Quote: ... Each DNA construct was validated by sequencing and transfected into cells using an electroporator (GenePulser xCell, Bio-Rad— Hercules, CA). Clonal cell lines were created by selection and limiting dilution ...
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bioRxiv - Biochemistry 2022Quote: ... by loading samples onto a 0.5X TBE 5% polyacrylamide gel prepared for a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad) using ProtoGel acrylamide (National Diagnostics) ...
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bioRxiv - Immunology 2022Quote: Live CD3+CD8-CD4+ cells were sorted (for gating strategy see supplemental figure 1A) into 384-well hard shell plates (Biorad) with 5 μl of vapor-lock (QIAGEN ...
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bioRxiv - Immunology 2022Quote: Live CD3+CD4+CD25+CD127low cells were sorted from fresh SF (Supplementary figure 1a) into 384-well hard shell plates (Biorad) with 5 μl of vapor-lock (QIAGEN ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... samples were separated by size on 12.5% SDS-PAGE gels run at 150V for 60 minutes using the Mini-PROTEAN Tetra Cell system (Bio-Rad). The proteins were transferred to a polyvinylidene fluoride membrane (PVDF ...
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bioRxiv - Systems Biology 2022Quote: ... Protein extracts derived from OBC1 cells were electrophoretically transferred onto nitrocellulose membranes using a Trans-blot Turbo transfer system (up to 25V, 7min) (BioRad). Membranes were probed with primary antibodies at 1:1000 dilution in 5% nonfat milk or BSA according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... and cDNA (1.0 μL) of cell or EV were prepared in 20 μL reaction and run on CFX96 qPCR machine (Bio-Rad). Gene expression level were first normalized to housekeeping gene GAPDH and then calculated with ΔΔCt method ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were transferred to polyvinylidene difluoride membrane (Milipore, Billerica, MA, USA) using a semi-dry chamber (Trans blot SD semi-dry transfer cell, Biorad) and blocked with 4% semi-skimmed milk in PBS (blocking buffer ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lysates were cleared by centrifugation and run on 10% SDS-polyacrylamide gels in a Mini-PROTEAN 3 cell apparatus (Biorad). Proteins were electroblotted onto Immobilon P membranes (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... snap frozen tumoroid cell pellets were lysed in Phosphatase-substituted RIPA buffer and run on a 10 % precast gel for P53 detection (BioRad). Protein levels of P53 (1:1.000 ...
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bioRxiv - Cell Biology 2022Quote: ... The whole cell lysate and crude cytoskeletons were resolved on 4-20% Mini-PROTEAN tris-glycine polyacrylamide gels (Bio-Rad), and Western blots were stained with 1:5000 diluted anti-beta-actin mouse monoclonal antibody (Cell Signaling Technology ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast concentration was adjusted to 4 × 108 cells/ml and added to the same amount of 1.2% low melting agarose gel (Bio-Rad) solution ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast suspensions (2 × 108 cells/ml) containing 0.6% low melting agarose gel were added to 50-well dispensable mold plates (Bio-Rad). Plugs were immersed in 10 ml of NDS (1% N-lauroyl sarcosinate sodium salt solution ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed and normalised for protein concentration using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Inc. # 5000001) and a spectrophotometer ...
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bioRxiv - Neuroscience 2021Quote: ... We ran 40 µg of protein on 10% gels using the Mini-PROTEAN Tetra Cell western blotting system (Bio-Rad). Anti-NaV1.1 (Ab5204a ...
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bioRxiv - Biophysics 2021Quote: ... 100 ng of pTrcHis plasmid (with or without transcribed complementary target sequences) were added to 50 μl of competent cells in a 0.2 cm-gap Gene Pulser® electroporation cuvette (BioRad) on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were transferred to 0.45 μM nitrocellulose membranes using Trans-Blot® Turbo™ Transfer System Cell system (Bio-Rad). The membrane was then blocked in 5% nonfat dry milk TBS 0.05% Tween 20 and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were prepared using an iScript qRT-PCR sample preparation reagent (50 μL/well) (Bio-Rad Labs, California, CA) [31 ...