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Citations for Bio-Rad :
1801 - 1850 of 2583 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). For RT-qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of whole cell extracts and tissue lysates were determined using a Bradford Protein assay (Bio-Rad). 50μg of sample proteins per well were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cell debris were spun down and the supernatant loaded onto a 1D SDS min-gel (BioRad Laboratories, US). We visualized the gel by Coomassie staining and excised the region corresponding to the molecular weight of Tdrd7 protein for subsequent MS-Western analysis.
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bioRxiv - Cancer Biology 2021Quote: 2 ⨯ 106 CAR T cells collected after 24-day expansion were resuspended with 1 ⨯ Laemmli sample buffer (BIORAD) containing 2.5% β-mercaptoethanol before sonication with 50% amplitude in Sonic Dismembrator (FisherBrand) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by electrophoresis at 17 V for 45 min at 4 °C (Mini-Sub® Cell GT, BIORAD). Slides were transferred to DNA precipitation solution for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates and virion lysates were resolved on 8 to 16% Mini-Protean TGX precast gels (Bio-Rad), transferred onto nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... or 12% SDS polyacrylamide gel electrophoresis (SDS-PAGE) using Mini- PROTEAN® Tetra Cell (Bio-Rad, CA, USA). After migration ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were washed with ice-cold PBS and lysed by using 100 μL lysis buffer (Bio-Rad). After confirming lysis by light microscopy ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were labeled with a mouse monoclonal anti-Chlamydia LPS antibody (BioRad CF 6J12, Hercules, CA, USA), rinsed with 1x PBS ...
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bioRxiv - Plant Biology 2021Quote: Twenty-five μL of electrocompetent Agrobacterium tumefaciens strain LBA4404 cells were electroporated in an electroporator (Bio-Rad Micropulser) according to the manufacturer’s instructions with 1 μL of Golden Gate assembled plasmid prep.
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bioRxiv - Molecular Biology 2020Quote: ... IPG strips were run upon SDS PAGE (12.5% acrylamide) using the Protean® II 2-D Cell (BioRad). Gels were then Coomassie blue stained (Phastgel Blue R ...
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bioRxiv - Cancer Biology 2020Quote: ... or a Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad; for mTOR signaling Western blots). Membranes were blocked in 5% non-fat dry milk or 5% BSA for phospho-epitope antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... for 16 h at 15 V using a Mini Trans-Blot® Cell (Art. No. 1703930, Bio-Rad) and 1x NuPAGE™ Transfer Buffer (Art ...
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bioRxiv - Microbiology 2022Quote: ... flow cytometry analysis was carried out to determine >55% of cells expressing the maturation marker CD35 (E11, Biorad) and >12% of cells expressing the proliferation marker CD71 (DF1513 ...
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bioRxiv - Cell Biology 2022Quote: Total protein concentration of cell or liver lysate was determined using the Lowry assay (Bio-Rad Laboratories, 5000112JA). Equal amounts of protein were loaded onto Tris-glycine sodium dodecyl sulfate-polyacrylamide gels ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were mixed with 4x Laemmli sample buffer and run on a 4–20% gradient gel (BioRad) and transferred to nitrocellulose filter (BioRad) ...
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bioRxiv - Bioengineering 2022Quote: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) was performed with MiniPROTEAN® Tetra Vertical Electrophoresis Cells (BioRad) using mini-PROTEAN® TGX™ precast gels (BioRad) ...
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bioRxiv - Systems Biology 2022Quote: ... Transfer was done using PVDF membranes and a Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad). Imaging was done with an Odyssey Fluorescence scanner (Li-COR ...
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bioRxiv - Neuroscience 2020Quote: ... Electrophoresis and protein transfer was achieved using Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad Laboratories, Inc. USA). After the transfer the blots were blocked for 2 h in 1 x Animal-Free Blocker (SP-5030 Vector Labs ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in agarose insert buffer and mixed 1:1 with 2% low-melting agarose (Bio-Rad). Gel inserts were solidified at 4°C for overnight and were stored in agarose insert buffer until analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... For cell lysis and further cDNA synthesis and Pre-Amplification we used the protocol supplied by Bio-RAD (http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6777.pdf ...
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bioRxiv - Plant Biology 2021Quote: ... Electro-competent Psa cells were transformed on the Gene Pulser Xcell™ Electroporation System (Bio-Rad, New Zealand), supplemented with sterile ...
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bioRxiv - Microbiology 2021Quote: ... Fixed cells were labeled with a mouse monoclonal anti-Chlamydia LPS antibody (BioRad CF 6J12, Hercules, CA, USA), rinsed with 1x PBS ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 0.4 V cm−1 in a Mini-PROTEAN® Tetra Cell (Bio-Rad, CAD). His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Cytokines from cell culture supernatants were quantified using Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-rad), IFN-β ELISA kit (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates (30 µg) were run approximately one inch into a 4-15% bis-acrylamide gel (Bio-Rad) and processed for in-gel digestion as previously described (60) ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates and virion lysates were resolved on 8 to 16% Mini- Protean TGX precast gels (Bio-Rad), transferred onto nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Systems Biology 2022Quote: Cell extracts (15 ug) were resolved by SDS-PAGE using 4-15% TGX precast gels (Bio-Rad #4561086) with tris/glycine/SDS buffer and transferred onto PVDF membranes using the Bio-Rad Mini-PROTEAN Electrophoresis System ...
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bioRxiv - Cancer Biology 2022Quote: An Aurum RNA mini kit was used to purify total RNA from the cells (Bio-Rad, Hercules, CA). For real time PCR experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed in PBS and fixed for 20 min in a 0.2% Coomassie Brilliant Blue (Bio-Rad) solution containing 50% methanol (Merck ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subjected to a single 1.8 kV pulse using the EC1 setting on a MicroPulser (Bio-Rad). Time constants were ∼ 4-5 msec ...
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bioRxiv - Cell Biology 2019Quote: ... Transfected parasites were then sorted using flow cytometry with a S3 Cell Sorter (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2019Quote: ... The RNA–protein complexes were transferred to nitrocellulose membrane using a Mini Trans-blot electrophoretic transfer cell (BioRad). The protein marker bands were overlaid with radioactive ink ...
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bioRxiv - Synthetic Biology 2020Quote: ... 25 μl of the resuspended cells were mixed with 25 μl of 2x Laemmli sample buffer (Biorad, CA) and boiled for 5 minutes at 95 T ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were harvested and subsequently subjected to subcellular fractionation as per manufacturer’s protocol (BIO-RAD, Hercules, CA, USA). Nuclear and cytoplasmic protein fractions were measured by Bradford protein assay (BIO-RAD ...
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bioRxiv - Microbiology 2019Quote: ... Whole cell lysates were resolved by SDS-PAGE in 4-20% gradient Mini-PROTEAN TGX gels (Bio-Rad) and transferred to 0.2 μm pore size nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Genomics 2019Quote: ... Single-cell ATAC-seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad) and SureCell ddSEQ Index Kit (12009360 ...
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bioRxiv - Bioengineering 2021Quote: ... was loaded with samples and run at 120V for 60 minutes with a Mini-PROTEAN Tetra Cell (BIORAD). The gel was included in a sandwich with a methanol activated PVDF membrane ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were sonicated using a cup sonicator and the protein concentration was determined using BioRad Protein Assay (BioRad). SDS dye was added to a 1X concentration and lysates (equal µg of protein ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified from the lysate of the bacterial cells by NGC Chromatography system (BioRad, CA, USA) with nickel column ...
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bioRxiv - Microbiology 2021Quote: ... proteins were transferred onto a blotting membrane (Immobilon-P) with a wet mini trans-blot cell (Bio-Rad). Blots were blocked for an hour in Tris-buffered saline with Tween 20 and 5% skim milk ...
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bioRxiv - Genomics 2020Quote: ... Electrophoresis was run for 15 minutes at 21V in a Mini-Sub Cell GT electrophoresis tray (Bio-Rad). Subsequently ...
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bioRxiv - Physiology 2021Quote: ... proteins were transferred from the gel onto a PVDF membrane using a Mini Tans-Blot Cell (Bio-Rad) overnight ...
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bioRxiv - Systems Biology 2019Quote: ... SDS PAGE separated 60µg of PC3 cell lysate per well with Precision Plus Protein Standards ladder (1610374, Biorad). Gel pieces were cut by razor blade ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5×106 cells per plug: plugs have been treated with Proteinase K (CHEF genomic DNA plug kit, Biorad) and restriction-digested by BamHI (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... in DPBS for 10 minutes at room temperature and imaged on a ZOE Fluorescent Cell Imager (Bio-Rad).
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bioRxiv - Microbiology 2019Quote: ... The resulting cell extract (INPUT) was used to determine the total protein concentration using Bradford assay (Biorad, USA). 10 mg of total proteins were mixed with Magnetic anti-FLAG beads (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Cell extracts were separated by SDS-PAGE on 7.5% polyacrylamide gel and transferred to nitrocellulose membrane (Bio-Rad) for immunodetection ...
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bioRxiv - Genomics 2021Quote: ... Single-cell ATAC-seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad) and SureCell ddSEQ Index Kit (12009360 ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysate protein samples (40 µg) were resolved on Criterion 4–20% gradient denaturing gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad ...