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Citations for Bio-Rad :
1601 - 1650 of 2583 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: BN-PAGE gels were transferred to PVDF membranes using a Mini Trans-Blot Cell (Bio-Rad). After wet-transfer ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed twice with cold wash buffer and analyzed using a ZE5 cytometer (Biorad) with acquisition chamber T= 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell extracts were sonicated 10 s and protein concentration determined using DCTM Protein Assay (Bio-Rad). Ten micrograms of proteins were loaded and run on NuPAGE 3-8% Tris-Acetate (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in monomer solution (1 x PBS, 2 M NaCl, 2.5% acrylamide (Bio-Rad), 0.15% Methylenbisacrylamide (Santa Cruz) ...
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bioRxiv - Physiology 2020Quote: ... conditioned media from cells was passed through columns containing AG1-X8 Anion Exchange Resin (Bio-Rad) and collected in scintillation vials ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cell lysate protein was quantified with a modified BCA protein assay using manufacturers protocol (Bio-Rad). Samples (20-30 μg ...
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bioRxiv - Microbiology 2019Quote: ... Electroblotting was carried out using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) for 2 h at a constant current of 200 mA ...
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bioRxiv - Microbiology 2020Quote: ... Gels were run at 4°C in a Protean II xi Cell electrophoresis system (Bio-rad) at 40 mA/gel with a running buffer consisting of 100 mM Tris-tricine pH 8.2 until the bromophenol blue loading dye was near the bottom of the gel ...
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bioRxiv - Immunology 2020Quote: cDNA was generated from sorted B cells by adding 4µl of iScript (Bio-Rad Cat# 1708891) and cycling according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the plate was placed on ice and the cells were lysed in 1X Laemmli buffer (Biorad) containing 5% β-mercaptoethanol (VWR) ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to PVDF (GE-Healthcare) membranes by Trans-Blot Semi-Dry Transfer Cell (Bio-Rad). Membranes were blocked with 5% BSA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: Whole cell lysates were collected in standard RIPA buffer and quantified using a Bradford assay (BioRad). Protein lysates (50µg ...
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bioRxiv - Genomics 2019Quote: ... Cell lysates (20 μg) were separated on 4–20% precast polyacrylamide gels (Bio-Rad, Hercules, CA) and were transferred to nitrocellulose membranes ...
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bioRxiv - Immunology 2020Quote: ... and single-cell sorted into 96-well PCR plates (free of DNase and RNase, Bio-Rad). The Plates were stored at −80 °C until BCR Cloning ...
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bioRxiv - Physiology 2021Quote: ... into the lanes of a 5% stacking-and 10% separating polyacrylamide gel (Biorad, MiniProtean Tetra cell). The proteins were separated at 60 V for 30 min and 150 V until the Hb fraction (∼16 kDa ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were transferred into ice-cold 2 mm electroporation cuvettes (Bio-Rad, Hercules, United States) and electroporated (2500 V ...
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bioRxiv - Developmental Biology 2021Quote: ... cultures were dissociated with Accutase and analyzed by flow cytometry using an S3 cell sorter (Biorad). Undifferentiated ESCs or iPS cells ...
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bioRxiv - Cell Biology 2021Quote: Measurements of cell density and viability were performed using trypan blue (Bio-Rad, Hercules, CA, USA) and an automatic cell counter (model TC20 ...
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bioRxiv - Microbiology 2020Quote: ... The cell suspension containing plasmid DNA was electroporated using a Gene Pulser electroporator (Bio-Rad, USA) with the following parameters ...
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bioRxiv - Cell Biology 2021Quote: ... Single cells were index sorted using a BD FACS Influx into 384-well hardshell plates (BioRad) that were pre-filled with 5 µL of light mineral oil (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with cold wash buffer and analyzed using a ZE5 cytometer (Biorad) with acquisition chamber T= 4°C ...
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bioRxiv - Genomics 2022Quote: ... Gels were ran at 100-200V in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Biorad 1658004). Proteins were transferred to Immobilon-FL PVDF (Millipore IPFL00010 ...
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bioRxiv - Cell Biology 2022Quote: ... Bands were visualized by chemiluminescence using enhanced chemiluminescent (ECL) substrate (BioRad, #170-5061/Cell Signaling, #12757S).
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bioRxiv - Microbiology 2022Quote: ... stained with Trypan blue using standard procedures and analyzed with a TC20TM Automated Cell Counter (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... 0.3% SDS and 20% methanol) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) set at 100 mA constant power with a volt limit of 25 V for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... the samples (cells with plasmid) were added to a 2mm-gap electroporation cuvette (Bio-Rad, USA). The sample was pulsed at 140V and 1,000 μF with an exponential decay on a Bio-Rad GenePulserXcell module ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were stained with the fluorescent inhibitor probe FAM-YVAD-FMK (FAM 660, BioRad, Germany) for 30 minutes at 37°C according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and cell lysates were resolved on Mini-PROTEAN TGX Stain-Free™ precast gels (Bio-Rad). For immunoblotting ...
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bioRxiv - Neuroscience 2022Quote: ... Isolated hBMSCs-derived cells were transfected using the Gene Pulser-II Electroporation System (Bio-Rad Laboratories). Electroporation was performed in a sterile cuvette with a 0.4-cm electrode gap (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2023Quote: ... 30μg whole-cell extracts were separated by SDS–PAGE and transferred onto Nitrocellulose membrane (Bio-Rad). After blocking in 5% milk in TBS-T ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stained with trypan blue and counted using Automated Cell Counter (BioRad, Hercules, California, USA). PBMCs were then cryopreserved in a cryovial in cell recovery freezing medium containing 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Molecular Biology 2022Quote: ... We dissociated the cells and extracted RNA using the Aurum Total RNA mini kit (Biorad, 7326820). We measured RNA concentration using a spectrophotometer and only included RNA that had an A260/280 >1.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Identical number of cells from each sample was collected and electrophoresed on polyacrylamide gels (Bio-Rad). Antibodies used were as follows ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the cell extracts was determined using the BCA assay kit (Bio-Rad). Protein concentration for lysates was adjusted to 2 μg/μl before adding 4X loading buffer (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... with BD FACS Melody instrument for the first round library and S3e Cell Sorter (Bio-Rad) for the second round library ...
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bioRxiv - Synthetic Biology 2023Quote: ... Equal amounts of whole cell protein were separated on a 15% SDS-polyacrylamide gel (Bio-Rad) by SDS-PAGE ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were transformed into onion (Allium cepa) epidermal cells using a particle delivery system (BIO-RAD) as previously described (Yang et al. ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates were loaded onto Any kD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad) and separated by electrophoresis ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: 25 μg of EVs or cell lysates was mixed with Laemmli SDS sample buffer (Bio-Rad), incubated 10 min at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... Specific DPCs in cells were detected using a dot blot (Bio-Rad, Bio-Dot Apparatus 1706545) and transferred directly to a nitrocellulose membrane by vacuum aspiration ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was then transferred to membrane via Trans-blot SD Semi-Dry transfer cell (Bio-Rad) and ran according to gel size ((2*gel area)/1hr = mA) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration in total cell lysates was determined using Protein Assay Dye Reagent Concentrate (Bio-Rad), normalized before denaturing in 4x LDS sample buffer (Thermo ...
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bioRxiv - Biochemistry 2023Quote: ... by electro-transfer (30 V constant overnight) in the Mini Trans-Blot cell module (Bio-Rad). The membrane was blocked for 1 h at 4 °C with 3% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: Cell lysates with 30 µg protein were mixed with 2x Laemmli sample buffer (Biorad, catalog #1610737) in a 1:1 ratio and heated at 95°C for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... and transferred onto a nitrocellulose membrane using Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, #1703930). Membranes were stained with Ponceau S ...
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bioRxiv - Biochemistry 2023Quote: ... Each sample was blank subtracted and zeroed at 600 nm using a TC20 cell counter (BioRad). The samples were prepared in biological triplicates ...
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates or recombinant protein were loaded on a 4-20% Criterion gel (BioRad, 3450032) for SDS-PAGE and transferred to PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lysate was resolved using SDS-PAGE and transferred to the nitrocellulose membrane (Biorad Laboratories) using a transfer system (Trans-Blot Turbo ...
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bioRxiv - Cell Biology 2023Quote: ... EVs and ENPs secreted by 20×106 cells were resuspended in Laemmli sample buffer (Bio-Rad), boiled 10 min at 95°C and run in 4% - 15% Mini-Protean TGX Stain-Free gels (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was transferred onto a nitrocellulose membrane using a Mini Trans-Bolt Cell (Bio-Rad Laboratories). Membranes were then stained with Ponceau S Staining Solution (Life Technologies ...