Labshake search
Citations for Bio-Rad :
1701 - 1750 of 1820 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Total protein (mg/mL) was determined for each sample using Bradford assay reagent (Catalogue # 5000002, Bio-Rad, Hercules, California, USA). Liver mitochondria (0.175 mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... software in 0.2 ml 96-well PCR plates (dot scientific inc.) sealed with transparent Microseal B Adhesive Sealer (Bio-Rad). Data were analyzed using the comparative Ct (2-ΔΔCt ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3x with 10 mL of TBS-T and developed with Clarity Max ECL substrate (Bio-Rad). Western blot images were taken with an Azure Biosystems C400 imager.
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bioRxiv - Bioengineering 2024Quote: ... The gels were stained under constant shaking at 80 rpm on an Orbital Shaker D0S-10L (NeoLab) using 50 mL Coomassie stain (BioRad) overnight and imaged using a c600 gel imager (Azure Biosystems).
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bioRxiv - Neuroscience 2021Quote: ... Samples were clarified by a brief centrifugation (10,000 g for 5 min at 4°C) and the supernatants were collected and diluted with 2× reducing Laemmeli buffer (Bio-Rad, Hercules, CA, USA, cat. #1610737). For preparation of cell culture lysates ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were run in a 2% agarose slab gel and stained with ethidium bromide for visualization by UV shadowing (Bio-Rad Molecular Imager Gel Doc XR+).
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bioRxiv - Biophysics 2021Quote: To extract lipids, purified SaPgsA (5.82 mg ml-1, 200 μl) was mixed with 40 mg SM2 Biobeads (Bio-rad, USA) and gently shaking overnight at 4 °C to remove detergent ...
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bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer B + 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer D ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer C + 400 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Each well was then coated with 150 µl of blocking buffer consisting of PBS and 40 mg/ml blotting-grade blocker (Bio-Rad). The plates were incubated with blocking buffer for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM β-mercaptoethanol) until the washes indicate a protein concentration of less than 0.1 mg/mL by Bradford assay (Bio-rad). The protein was eluted with elution buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Additional one-ml samples were used for total protein determination using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). β-galactosidase assays were performed as described previously (38) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected after centrifugation at 15,000 ×g for 15 min at 4°C and applied to a 20 mL open column (Poly-Prep Chromatography Columns; BIO-RAD) containing 500 µL of Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
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bioRxiv - Biochemistry 2022Quote: ... cell density was between 4-5 × 106 cells/mL with >95% viability as determined using a TC20 automated cell counter (Bio-Rad). Cells were diluted with warm Expi293 Expression Media to 2 × 106 cells/mL in 42.5 mL ...
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bioRxiv - Biophysics 2022Quote: ... Aqueous solutions in BRB80 pH 6.9 were introduced in the chamber in the following sequence: 20 μL of 0.05 mg/mL anti-tubulin antibody (Bio-Rad Laboratories) for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 ml of supernatant was diluted with 3 ml of distilled H2O and applied to freshly prepared Dowex columns (AG1-X8; Bio-Rad). Columns were washed twice with distilled H2O ...
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bioRxiv - Biochemistry 2021Quote: ... The cell extract was clarified by filtering through 0.4 µm and 0.22 µm filters prior to loading onto ~4 mL of Ni-NTA resin (Profinity™ IMAC resin, Bio-Rad). To allow the His-tag protein to bind the resin it was incubated overnight at 4°C on a rolling shaker ...
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bioRxiv - Microbiology 2022Quote: ... Each well was then coated with 150 µl of a blocking buffer consisting of PBS and 40 mg ml-1 blotting-grade blocker (Bio-Rad). The plates were incubated with the blocking buffer for 1 h at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... The fractions were analyzed by SDS-Page and fractions containing the protein of interest were pooled and loaded onto a 5 mL Mini-profinity IMAC column (Bio-Rad). Metal affinity chromatography was performed as previously described for the TEV protease ...
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bioRxiv - Microbiology 2019Quote: ... The gel was stained with 5 μg/ml ethidium bromide for 20 min and imaged using a Chemidoc XRS+ (Bio-Rad).
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bioRxiv - Synthetic Biology 2020Quote: ... 560 µl of FBP-sepharose were mixed with 8.4 mL of 20 nM CAL Fluor Red 610-labelled RNAs in a Econo-Column Chromatography Column (BioRad, Hercules,CA), and the column washed with CB until no fluorescence change was detected in the flow-through ...
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bioRxiv - Neuroscience 2020Quote: ... Bovine Serum Albumin (BSA) with concentrations 0.125 to 2.0mg/mL from a standard set served as protein standards (BioRad, Cat. Number 5000207). Then ...
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bioRxiv - Synthetic Biology 2020Quote: ... The samples were run with 5 mM H2SO4 at 0.6 mL/min on an Aminex HPX-87H (Biorad Inc., CA, USA) column at 50°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Immunology 2020Quote: ... Iodixanol was removed from samples by size exclusion gel filtration using a 20 ml column (Bio-Rad Laboratories, Hercules, CA, USA) packed with 15 ml Sephadex g100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: Polymerizing gel solution was made by combining 15 mL of 9.5% gel solution with 133 uL of 10% Ammonium Persulfate Solution (APS) and 13.3uL TEMED (BIO-RAD #161-0700) into a beaker under a fume hood ...
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bioRxiv - Biochemistry 2020Quote: ... was applied to Sephadex G-10 column (85 ml, fractionation range <700 Da) fitted with Biologic duo flow instrument (Bio-Rad) at a flow rate of 6 ml/hr ...
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bioRxiv - Microbiology 2020Quote: ... The gel was stained with 5 μg/ml ethidium bromide for 20 min and imaged using the ChemiDoc XRS+ system (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Each well was then coated with 150 µl of a blocking buffer consisting of PBS and 40 mg ml-1 blotting-grade blocker (Bio-Rad). The plates were incubated with the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... ethidium bromide at a final concentration of 0.46 μg mL−1 was used and the DNA was visualized under UV light using a GelDoc XR+ system (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... on ice for 24–36 h and then removed through glutathione sepharose 4B beads (5 mL bed volume) packed twice in Poly-Prep® Chromatography Columns (BioRad). UtrCH-sfGFP and LifeAct-sfGFP were further purified through gel filtration column chromatography (HiLoad 16/600 Superdex 75 pg ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant proteins lacking the 6His tag were further purified via reverse IMAC using 0.8 ml Ni2+- loaded Chelating Sepharose Fast Flow resin packed into poly-prep® chromatography columns (cat. Number 7311550 – Bio-Rad) and prepared as above ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked by incubating in 15 mL of blocking solution (5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... gene were electroporated on 8×106 Vero-E6 cells resuspended in 0.8 mL of Mirus Bio™ Ingenio™ Electroporation Solution (#MIR50114) using the Gene Pulser Xcell Electroporation System (#1652660, Biorad) with a pulse of 270V and 950 μF ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
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bioRxiv - Molecular Biology 2023Quote: ... LAMP reaction products were analysed on 2% agarose gels (65V for 3 hr) stained with ethidium bromide (10 mg/mL) and viewed on Gel Doc XR Imaging System (BioRad, USA).
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bioRxiv - Neuroscience 2022Quote: ... Gel was rinsed once in ultrapure water (AmericanBio) and incubated in ∼50 mL of Coomassie blue R-250 staining solution (Bio-Rad) for 1 hour incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... A final concentration of 0.1 μg/mL DAPI was then added for 10 mins and digital images were captured using ZOE Fluorescent Cell Imager (Bio-Rad). For negative controls ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplification products were analysed on 1% agarose gel (80V for 1 hr) stained with ethidium bromide (10 mg/mL) and viewed on Gel Doc XR Imaging System (BioRad, USA).
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bioRxiv - Molecular Biology 2023Quote: ... gels were stained with ethidium bromide (5 µL of 10 mg/mL ethidium bromide (EtBr) solution in 100 mL TAE) and imaged in a Gel Doc XR + System using the Image Lab software (Bio-Rad). Each experiment was carried out in triplicate.
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bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2023Quote: Hydrazine synthase α-subunit was purified by applying the 200 mM NaCl fraction from the Q-sepharose column on a 50 ml hydroxyapatite type II 40 μm (Bio-rad) column (XK 26/20 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing ADDoCoV were pooled and further purified by ion exchange chromatography (IEX) using a 5 ml Bio-Scale Mini Macro Prep High Q (Bio-Rad) equilibrated in Buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 20 minutes) and the clear lysate was purified on HisTrap HP 1 ml affinity column (Cytiva) by FPLC system (NGC™, BioRad). The protein was eluted from the column using a linear gradient (Elution buffer ...