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Citations for Bio-Rad :
1601 - 1650 of 1820 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... membrane was incubated 1 min in 1 ml enzyme solution (Clarity™ Western ECL, BIORAD, Les-Ulis, France) and 1 ml Luminol/enhancer solution (Clarity™ Western ECL ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies were used: mouse anti-talin1 (clone 97H6, Bio-Rad: MCA4770, Lot#:1803, 1 μg/ml), rabbit anti-zyxin (B71 ...
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bioRxiv - Immunology 2022Quote: ... incubated for five days at 4°C in 40 mL of hydrogel monomer solution [4% acrylamide (Bio-Rad), 0.05% bis-acrylamide (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... by concentration and redilution and then concentrated to 25 mg/ml as determined using Bradford’s reagent (Bio-Rad). BsDHFR was supplemented with NADP+ (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a 1 ml HisTrap (Cytiva) column connected to NGC Quest 10 (Bio-Rad), previously equilibrated with Buffer A (25 mM Tris–HCl pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR was performed using 3.5 μl of diluted cDNA and 5.5 μM primers listed in Table 2 (C.-M. Lee and Thomashow, 2012; Wu et al., 2008)using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, cat# 1725121) with the CFX 384 TouchTM Real-Time PCR Detection System (Bio-RAD) ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted from SSLs using 350 μL of RIPA buffer and was purified using the Ready Prep 2-D cleanup kit (Bio-Rad, Hercules, CA, USA); the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... This mix was transferred to a pre-chilled 2 mm electroporation cuvette and electroporation was performed (2.0 kV, 25 μF, 200 Ω) using a Gene Pulser Xcell (Bio-Rad Laboratories, Hercules, CA) electroporation sys-tem ...
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bioRxiv - Biochemistry 2019Quote: ... Three hexasaccharides with unsaturated uronic acid at the non-reducing end were chemically synthesized using modified reported procedures and the reaction products were desalted over a Bio-Gel P-2 column (Bio-Rad, Hercules, CA, USA) without further purification ...
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bioRxiv - Biochemistry 2020Quote: ... were incubated together at RT for 5 min in the presence and absence of 5 mM 2-OG prior to loading on the analytical ENrich 650 column (BioRad Laboratories, Inc., Hecules, USA). The column was equilibrated with 50 mM Tris/HCl and 150 mM NaCl and supplemented with 5 mM 2-oxoglutarate when the complexes were preincubated in the presence of 5 mM 2-oxoglutarate and the applied proteins isocratic eluted with the respective buffer with a flow rate of 1 ml min-1 ...
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bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
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bioRxiv - Biochemistry 2020Quote: To visualize protein expression 1 μL of the TXTL reactions was mixed with 2 μL of water and 3 μL of 2x Laemmli loading dye (Bio-Rad, Hercules, CA, USA) and incubated at 90°C for 3 min ...
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bioRxiv - Cell Biology 2019Quote: ... Separation in the second dimension was accomplished using an 10% SDS-PAGE in a Protean II xi 2-D Cell (Bio-Rad, Hercules, CA, USA) at 50 mA until the bromophenol blue reached the bottom of the gel ...
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bioRxiv - Synthetic Biology 2020Quote: The constructs were transformed into Agrobacterium tumefaciens strain pGV3850 by electroporation (2 mm cuvette, 2.5 kV, 400 Ω, and 25 μF) in a Bio-Rad GenePulser (Bio-Rad, Hercules, CA, USA). Cells were incubated at 28°C for 2 h after 200 μl LB media was added ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of cells were mixed with PCR products and transferred to a precooled 2 mm gap electroporation cuvette (Bio-Rad Laboratories, Hercules, CA). A BTX Gemini X2 instrument was used for electroporation with the following settings ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Neuroscience 2022Quote: ... Solid phase specificity was confirmed with dot blots of serial antigen dilutions (2 μL) applied to nitrocellulose membrane (Bio-Rad 0.45 µm; Fig. 1b). Membranes were allowed to air dry (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of total RNA was reverse-transcribed to single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad Hercules, CA, USA). RT-qPCR was run on Lightcycler 480 (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... We mixed 10 μL of each fraction with 2 μL of denaturing RNA loading dye and loaded it into a 1.0 cm well PAGE casting plate (Bio-Rad Laboratories, Mississauga, ON, Canada). Urea-PAGE (7.5% ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... DNA was diluted to 10 ng/μl and 2 μl were used in each RT-qPCR reaction with 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) and 400 nM of forward and reverse primers ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg of dsDNA were added to the competent cells and then transferred to chilled 2 mm gap electroporation cuvettes (Bio-Rad Laboratories GmbH, Germany). Electroporation transformation was done with a single pulse at 1.8 kV ...
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bioRxiv - Developmental Biology 2023Quote: ... under reducing conditions using 2-ME and transferred onto polyvinylidene difluoride (PVDF) membrane using the Trans Blot Turbo system (Bio-Rad, Hercules, CA, USA). After blocking with 10% skim milk (Becton Dickinson ...
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bioRxiv - Physiology 2020Quote: ... Overall protein concentration (mg/ml) was measured according to the Bradford method (Bradford, 1976) using BioRad stock (BioRad, Finland) diluted with dH2O (1:5 ...
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bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the appropriate resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively with buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The reconstitution sample was nutated for 1 h at 4°C before addition of 0.2 g/mL SM2-BioBeads (BioRad), and the reconstitution sample was further nutated overnight at 4°C before removal of the biobeads ...
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bioRxiv - Synthetic Biology 2020Quote: ... Samples were loaded in MicroAmp™ Fast Optical 96-Well Reaction Plate (0.1 mL) and the experiment was carried out with a CFX96 Touch Real-Time PCR Detection System (BioRad) machine ...
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bioRxiv - Biophysics 2019Quote: ... Solutions were introduced in the chamber in the following sequence: 20 μL of 0.05 mg/mL anti-tubulin antibody (BioRad) for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... The BRPF1 bromodomain protein was purified on glutathione agarose resin in a 25 mL Econo-Column® (Bio-Rad) by first washing the beads extensively with wash buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Corresponding fractions for each chromatogram between 12 and 18 ml with an interval of 0.5ml were analyzed by SDS-PAGE (Biorad) and Instant blue coomassie staining (Expedeon).
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bioRxiv - Cancer Biology 2021Quote: ... Lysates prepared at a concentration of 1 mg/ml and supplemented with 4x Laemmli Sample Buffer (Bio-Rad: 1610747) supplemented with fresh 2-mercaptoethanol (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein concentrations in the cell lysates (mg/mL) were assayed using the Bradford protein assay (Bio-Rad, Hercules, CA) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... purified on Ni-NTA resin (G-Biosciences, #786-940 in 5 ml Bio-Scale™ Mini Cartridges, BioRad #7324661), then dialyzed ...
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bioRxiv - Microbiology 2020Quote: ... and 150 ml Protein A Sepharose (GE, 17-5138-01) in a Poly-Prep Chromatography Column (BioRad, 731-1550). Fabs were generated by Papain cleavage and purified by the use of the Pierce Fab Preparation Kit according to the manufacturers’ instructions (Thermo Scientific - #44985) ...
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR analysis was performed in a total volume of 15 mL containing 1x iQSYBR GreenSupermix (Bio-Rad), 500 nM concentration of each PCR primer and 2 μL of 15x diluted cDNA templates using CFX96 real-time PCR system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... Stock concentration of SB225002 was diluted to a working concentration of 0.2mg/ml with 0.25% (v/v) Tween 20 (Bio-Rad) in 1xPhosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2019Quote: ... β-galactosidase activity units were normalized to total proteins per ml as determined using Bradford assay reagents (Bio-Rad). Assays were performed in biological triplicates.
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bioRxiv - Microbiology 2020Quote: ... 20 mL blocking buffer supplemented with 5 μL of horseradish peroxidase (HRP) conjugated rabbit anti-mouse secondary antibody (BioRad) was added and incubated for 2 hours at room temperature with agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the Amylose resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively ...
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bioRxiv - Cell Biology 2019Quote: ... the gels were stained with 0.4 µg/mL ethidium bromide in water for 10 min and visualized using the ChemiDocTM XRS+ (Biorad) imaging system ...
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bioRxiv - Microbiology 2022Quote: ... A 15% SDS-Tricine gel was made by mixing: 2.5 mL 30% acrylamide/Bis solution 37.5:1 (BioRad #1610158), 1.25 mL gel buffer (1.5 M Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... in water and 3.75 μL of sample was mixed with 0.18 μL of 25 mg/mL primers and 7.5 μL of iQ SYBR master mix (Bio-Rad). 15 μL reactions were analyzed with a CFX connect real-time PCR detection system in technical duplicate and at least biological triplicate ...
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bioRxiv - Microbiology 2022Quote: ... reagent in 0.2 mL PCR 8-well strips and heated to 100 °C for 10 min (T100; BioRad, Australia) and used immediately ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was normalized to 1 mg/mL using 0.1% Azo buffer by the Bradford assay (Bio-Rad). Samples were reduced with 30 mM DTT for 1 h and subsequently alkylated with 30 mM iodoacetamide for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... A conjugate pad (Ahlstrom, A6614) was soaked in a solution of 30 µg/mL IRDye 800CW Streptavidin (Bio-Rad), 2.5% BSA ...
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bioRxiv - Microbiology 2023Quote: ... We added 1.0 mg of various CRISPRi plasmids to the 0.2 ml fusobacterial competent cells in a chilled cuvette (0.1 cm electrode gap, Bio-Rad), allowing it to rest on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... aureus strains were grown for 18 hours overnight in TSB before diluting them to an OD of 0.05 (A600) in 7.5 mL of Chelex (Bio-Rad)-treated TSB in a 30 mL capacity culture tubes as described previously (44) ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was loaded onto a 5 mL Bio-Scale Mini Nuvia IMAC Ni-charged Cartridges (Bio-Rad) which was pre-equilibrated with 5 column volumes (CV ...
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bioRxiv - Genetics 2023Quote: ... followed by purification and size selection on 1% agarose gel with .6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked for 1hr in PBS with 1mg/ml and 0.1% triton and then stained overnight with anti-alpha Tubulin antibody (1:100, MCA78G, BioRad). Cells were then washed ...