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Citations for Bio-Rad :
1301 - 1350 of 1820 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells and plasmids in 10% glycerol were first heat-shocked at 56°C for 2 min followed by immediate electroporation in 0.1-cm cuvette (Bio-Rad) using electroporation program of 2.5 kV and time constant of 2.3-2.5 ms on the Bio-Rad Micropulser ...
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bioRxiv - Microbiology 2024Quote: ... 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad). Samples and plasmid standards were amplified using the following protocol ...
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bioRxiv - Biophysics 2023Quote: ... and yeast cell growth was monitored by measuring the OD600 at 2 h increments in a microplate reader (Benchmark Plus, BioRad). The remainder of the 10 ml cultures was used to assess Pgp expression by Western blot analysis of microsomal membrane preparations using the monoclonal C219 anti-Pgp antibody.
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bioRxiv - Molecular Biology 2020Quote: ... the total protein concentration was adjusted to 1.8 mg/ml using Bradford reagent (Bio-Rad) and 1 ml aliquots were separated over a Superdex 200 16/60 equilibrated with 20 mM Tris-HCl pH 7.5 and 50 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... at a 1 ml flow rate using a NGC Quest 10 chromatography system (Bio-Rad). Buffer A was 5% acetonitrile 0.01M ammonium bicarbonate ...
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bioRxiv - Synthetic Biology 2019Quote: ... The nickel sepharose-lysate suspension was poured onto a 10 mL disposable column (Bio-Rad), and the settled resin washed with 20 column volumes of a first wash solution (50 mM KH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: A 10% Tris/Glycine/SDS Buffer solution was prepared with 100 mL buffer (Bio-Rad) in 900 mL tissue culture grade water ...
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bioRxiv - Biochemistry 2020Quote: ... The collected beads were poured into a 25 mL Econo-Column Chromatography Column (Bio-Rad) and washed with four column volumes of wash buffer (20 mM Tris–HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The last wash was conducted in a disposable 10 ml Poly-prep column (Bio-RAD) and the protein was eluted from the beads with elution buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... Detergent was removed from the reaction by addition of 0.1 g/mL Biobeads (Bio-Rad) and incubation at 4 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The 3 mg/mL protein sample was injected onto NGC Liquid Chromotography System (Bio-Rad) equipped with a HiPrep Sephacryl S-500 HR column equilibrated in FPLC buffer and separated into 156 ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... Sly1-bound resin was collected in a 25 mL disposable Econo-Pac column (Bio-Rad) by gravity and washed with 25 mL of SLY1 buffer supplemented with 50 mM imidazole at pH 7 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The slurry was applied to a 10-mL Poly-Prep chromatography column (Bio-Rad, USA), which was then washed with 10 mL of purification buffer B (purification buffer A with 50 mM imidazole) ...
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bioRxiv - Biochemistry 2020Quote: ... the beads was transferred to a gravity-flow Econo column (49 ml volume; Bio-Rad) and washed with 2 column volumes (CV ...
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bioRxiv - Bioengineering 2019Quote: ... generated droplets or Gelbeads were extracted into PCR tubes (0.2 mL individual PCR tubes, BioRad) and incubated in a thermal cycler (T100 ...
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bioRxiv - Biophysics 2022Quote: ... lysate-resin suspension was loaded onto a 20 ml polypropylene gravity-flow column (Bio-Rad). After collecting the flow through ...
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bioRxiv - Biophysics 2022Quote: ... lysate-resin suspension was loaded onto a 20 ml polypropylene gravity-flow column (Bio-Rad). After collecting the flow through ...
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bioRxiv - Biochemistry 2022Quote: ... Detergents were removed by the sequential addition of 60 mg/mL Biobeads SM2 (Bio-Rad) for three times within 3 h ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His6 was then purified using a pre-packed 5 ml IMAC cartridge (Bio-rad) on a Bio-rad FPLC ...
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bioRxiv - Microbiology 2022Quote: ... Membrane pellets were resuspended at 11 mg/ml (as determined by Bradford assay, Bio-Rad) in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... Detergents were removed by incubation with 0.8 mg ml-1 Bio-Beads SM2 (Bio-Rad) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Detergents were removed by incubation with 0.8 mg ml-1 Bio-Beads SM2 (Bio-Rad) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with PBST containing 1% BSA and 22.5 mg/mL glycine (Bio-Rad 1610718) for 30 minutes at 23°C ...
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bioRxiv - Cell Biology 2023Quote: ... and cell density (cells/ml) was measured by an automated cell counter (TC20, Bio-Rad). The culture was diluted by medium lacking nitrogen and carbon sources to a density of 1×106 cells/ml ...
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bioRxiv - Biophysics 2022Quote: ... UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD). VDAC1 Porin was used as a mitochondrial loading control and detected by a mouse anti-VDAC1 0.1 μL/mL (16G9E6BC4 - Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification of cell number per ml was measured on a TC20 Cell Counter (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA) and 4 ml 1:6 diluted cDNA template along with 200 nM forward and reverse primers in a total volume of 10 ml per reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... with 0.2 ml Flat PCR Tube 8-Cap Strips (Bio-Rad™, optical, ultraclear, #TCS0803). Optimum amplification conditions were determined for each by amplifying six concentrations of a 5× dilution series of MG1655 pJK14-Tn4rev pZA31-mCherry-tnpB using two-step amplification with a thermal gradient of 55–75°C ...
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bioRxiv - Physiology 2023Quote: ... Matching slides per animal were immunofluorescence (IF) stained for cd68 (5 µg/mL, MCA1957, BioRad) followed by donkey anti-rat CF750 (2 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Protein-bound resin was then applied to a 10-mL chromatography column (BioRad, Hercules, CA) and washed twice with 10 mL wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2023Quote: Overnight cultures in TSB were diluted 100-fold into 1 mL of Chelex (Bio-Rad)- treated TSB with the addition of 25 μM zinc acetate ...
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bioRxiv - Biochemistry 2023Quote: ... before rinsing quickly with 2x10 mL TBS before treating with clarity ECL substrate (Bio-Rad). Signals were detected using a Bio-Rad ChemiDoc Imaging System ...
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bioRxiv - Biochemistry 2023Quote: ... The eluent was then applied to a 10-mL self-assembled HAP column (Bio-Rad), washed with 50 mL resuspension buffer ...
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bioRxiv - Microbiology 2024Quote: ... at 4 °C for 30 min in a 10 ml poly-prep column (Bio-Rad). The column was then stabilized for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and counted in the presence of Trypan Blue using the TC20™ Automated Cell counter (Bio-Rad) (24h floating cell count) ...
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bioRxiv - Cell Biology 2020Quote: Cells cultured in each well of a 6-well plate at 80% confluency were washed three times with PBS and harvested/resuspended in 150–200 μl of 2× Laemmli buffer (Bio-Rad). The cell lysate was boiled for 10 min and loaded onto an SDS gel ...
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bioRxiv - Cell Biology 2020Quote: ... Relative mRNA levels of the indicated genes were calculated by the 2-DDCT method (Bulletin 5279, Real-Time PCR Applications Guide, Bio-Rad), using GAPDH expression as endogenous control ...
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bioRxiv - Genetics 2021Quote: ... qPCR was carried out using BioMaster HS-qPCR SYBR Blue (2×) reagent kit (Biolabmix; https://biolabmix.ru/en/) and CFX96™ Real-Time PCR Detection System (Bio-Rad). We used the following thermal cycling conditions ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 2-3 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then diluted 1:10 and 2 µL of diluted cDNA were used for RT-qPCR along with 5 µL of iTaq universal SYBR mix (Bio-Rad), 1.5 µL of primers (1.5 µM mix of forward and reverse ...
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bioRxiv - Molecular Biology 2021Quote: ... was used with the primers listed in Supplementary Information Table 2 and samples were run on CFX96 Real-Time PCR Detection System (Bio-rad).
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bioRxiv - Microbiology 2020Quote: ... concentration of total aerobic GNB and of MDR-GNB were determined by plating serial dilutions (pure, 10−2, 10−4) of initial faeces or EA sample onto Drigalski agar (Bio-Rad) with or without 1mg/L cefotaxime ...
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bioRxiv - Immunology 2021Quote: ... Lysates were finally adjusted to a final concentration of 2 µg/µl with 4X Laemmli buffer (Bio-Rad, Hercules, CA, USA) containing 10% β-Mercaptoethanol.
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bioRxiv - Microbiology 2019Quote: ... 250 µl packed cells were combined with 250 µl of 1 × Cytomix in a 2 mm transfection cuvette (Bio-Rad Laboratories). The precipitated and resuspended DNA was added to the cell suspension in the cuvette ...
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bioRxiv - Biochemistry 2019Quote: ... MenD mutants were generated using the pYUB28b-menD construct and oligonucleotide primers (Supplementary Table 2, Integrated DNA Technologies) with iProofTM high-fidelity DNA polymerase (Bio-Rad). The PCR products were then treated with DpnI and ligated using T4 DNA ligase (Roche) ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with 1:200 rabbit-anti NF-B p65 polyclonal antibody (Pierce) (2 h) and 1:5000 Goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) (1 h) ...
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bioRxiv - Microbiology 2019Quote: ... was determined for different genes (primers are listed in Table 2) by quantitative PCR (qPCR) using iTaq SYBR Green (Bio-Rad) with 400 pg of cDNA and 0.25 μM each primer in 10 μL reactions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We then performed quantitative PCR (qPCR) using 2 ng of cDNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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bioRxiv - Biophysics 2021Quote: ... The mixture was diluted to ~30 mL with sample buffer and stirred at 4°C for 30 min prior removal of C12E8 by adding 4 g of Bio-Beads SM-2 (Bio-Rad) in stages of 0.5 ...