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Citations for Bio-Rad :
1651 - 1700 of 1820 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The enzyme was purified using a 5-mL Ni-charged EconoFit Nuvia IMAC Column (Bio-Rad, Hercules, CA, USA) and a linear gradient of 10 - 250 mM imidazole in binding buffer (100 mL in total at 2.5 mL/min flow rate) ...
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bioRxiv - Molecular Biology 2024Quote: 0.2 – 1 mL (sufficient to cover the sample) of 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA, USA) with 0.2% Tween20 and 0.1 mg/mL proteinase K (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed in a Bio-Rad Criterion TM Vertical Electrophoresis Cell at 90-120V for 1-2 hours and then transferred to a PVDF Transfer Membrane (#88518, Bio-Rad, Hercules, CA, United States) using the Bio-Rad Trans-Blot Turbo Transfer System ...
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bioRxiv - Plant Biology 2021Quote: ... the Agrobacterium-infiltrated leaves were visually inspected at 2-5 days post infiltration and/or the fluorescence of the infiltrated leaves were monitored at 1-2 d post infiltration under the Pro-Q Emerald 488 in the ChemiDoc™ Gel Imaging System (Bio-Rad, Hercules, CA, USA) as described previously (Yoon and Rikkerink 2020).
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 h at room temperature in HRP conjugated IgG anti-rabbit secondary antibody (Bio-Rad 170-65-15, 1:3000) or Cy5 conjugated IgG anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories Europe Ltd. ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using 2 × T5 Fast qPCR Mix (SYBR Green I) with a Bio-Rad CFX Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: The pure cultures were grown in TSAYE plates for 24 h and DNA was extracted using 2% Chelex solution (Chelex 100 resin, Bio-Rad laboratory, Hercules, California, USA). The colonies (2-3 mm ...
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bioRxiv - Microbiology 2020Quote: ... in oral swabs of positive and negative subjects for SARS-CoV-2 using magnetic bead-based multiplex immunoassays (Bio-Plex Pro™ human cytokine 27-plex panel, Bio-Rad Laboratories, Milan, Italy) according to the pre-optimized protocol 15 ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Genomics 2021Quote: ... Quantitative PCR was performed in triplicate and the 2−ΔCt method was used to calculate the expression of RUNX3 relative to β-actin (ID Assay qHsaCED0036269, Bio-Rad Laboratories, Kidlington, UK).
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bioRxiv - Microbiology 2019Quote: ... for 5 min in 2 ml screw-cap vials with 2 g of glass beads (2mm) and 200 μl of buffer containing 1 × TE buffer and Chelex®-100 (Bio-Rad Laboratories, CA, USA). Samples were then incubated overnight at 56 °C with 10 μl of Proteinase K (20mg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... and EV/HSP27 mixtures containing 2 µg of HSP27 at the indicated w/w ratios were mixed with native sample buffer (cat#1610738, Bio-Rad Laboratories Inc., Hercules, CA) and loaded into the gel lanes ...
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bioRxiv - Immunology 2023Quote: Plasma from control and infected mice was used at a 1:2 and 1:4 dilution with the Bio-Plex Pro Mouse Cytokine 23 Plex Immunoassay (Bio-Rad Laboratories, Inc., CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked in serum and then incubated in primary antibody for 2 hr at RT (CD-68: 1:500, Bio-Rad clone FA-11, MCA1957GA), and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG ...
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bioRxiv - Biochemistry 2023Quote: ... The eluate was incubated for 60 min at room temperature with 50 mg of prewashed SM-2 Bio-Beads (Bio-Rad Laboratories, Hercules, CA, USA) under overhead rotation to ensure detergent removal.
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bioRxiv - Immunology 2023Quote: ... was performed using 2×RealStar Green Fast Mixture (GenStar, Beijing, China) in a CFX384 Real-Time System C1000 Touch Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, USA). The relative transcription levels of the genes of interest were normalized against the expression of GAPDH and calculated using the ΔΔCT method ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were co-encapsulated with Drop-Seq beads (ChemGenes: lot 083117) in 1 nL droplets using 15000 mL/hr oil (QX200, Biorad), 4000 mL/hr cell suspension and 4000 mL/hr bead flow rates (droplet generation frequency 2 kHz) ...
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bioRxiv - Developmental Biology 2022Quote: ... the gel was incubated in 1x TBE for 10 min and after was stained with Green Safe reagent dye (5 µL /100 mL of TBE 1x) for 30 min and visualized on ChemiDoc XRS+ system (BioRad).
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bioRxiv - Molecular Biology 2020Quote: Polymerase Chain Reaction (PCR) was carried out in 0.2 mL volume PCR in a thermal cycler (T100 Thermal cycler, Biorad, USA). HF Phusion Master mix (M0531S ...
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bioRxiv - Plant Biology 2019Quote: ... The exchanged sample was diluted to either 0.8 or 0.4mg/ml via the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, CA). Samples were divided into 100µL aliquots and flash frozen with liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Gel was stained for 1 h with 0.1 μg/ml ethidium bromide and visualized by ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... The membrane was let dry and thereafter blocked on a shaker for 10 min in 15 ml blocking buffer (1% casein in TBS, BioRad). The blocking solution was discarded ...
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bioRxiv - Biochemistry 2020Quote: ... The resin was resuspended in 5 mL of extraction buffer and transferred to a gravity flow column (Biorad, Hercules, CA). After loading ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
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bioRxiv - Molecular Biology 2020Quote: ... Mean fluorescence intensity was measured to calculate final concentration in pg/mL using Bioplex200 and Bioplex Manager 5 software (Biorad).
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bioRxiv - Cancer Biology 2022Quote: ... and the pellet was resuspended in 1 ml of BM1* and cells were counted using a TC20 Automated Cell Counter (BioRad). Single-cell suspensions were mostly re-seeded at the same density as their parental/previous generation/passage.
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bioRxiv - Microbiology 2020Quote: ... Protoplast concentration was adjusted to 4 × 108 cells/ml and added to the same amount of 1.2% low melting agarose gel (Bio-Rad) solution ...
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bioRxiv - Microbiology 2020Quote: ELISA plates were coated overnight at 4°C with 0.1 µg/mL of mouse anti-human IgG (human CH2 domain with no cross-reactivity to rhesus macaque IgG; clone R10Z8E9; BioRad) and then blocked for 2 hours ...
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bioRxiv - Biophysics 2019Quote: ... Residual Ca2+ from this solution was removed by passing 100 mL of TLK buffer over 5 g of pH-neutralized Chelex 100 resin (BioRad) in a column three times at a flow rate of ~3 mL/min ...
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bioRxiv - Microbiology 2021Quote: ... Cells were diluted into 5 ml 1X PBS and sorted and analysed with a S3e(tm) Cell Sorter and ProSort(tm) Software (BioRad). Fluorescence intensities of cells were measured using 488 nm and 561 nm lasers ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Biochemistry 2021Quote: A 9.5% gel solution was prepared by combining 47.5 mL 30% Bis/Acrylamide solution 29:1 (BIO-RAD, #161-0156) with 41mL MilliQ Water ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli Mach1-T1 cells was collected in 1.5 mL centrifuge tubes and then visualized under bright field and imager (ChemiDoc MP Imaging System, Bio-Rad). For imaging the fluorescence of mCherry ...
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bioRxiv - Cell Biology 2021Quote: ... Mean fluorescence intensity was measured to calculate final concentration in pg/mL using Bioplex200 and Bioplex Manager 5 software (Biorad).
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated in a 5 ml 1:1 A:B solution of Clarity Western ECL substrate (BioRad cat. #170-5060) for 5 minutes at room temperature with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants were transferred to pre-chilled low-adhesion 1.5 mL tubes and quantified by Bradford (cat. no. 5000006, Bio-Rad). Prior to loading ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants were transferred to pre-chilled low-adhesion 1.5 mL tubes and quantified by Bradford (cat. no. 5000006, Bio-Rad). After quantification ...
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bioRxiv - Bioengineering 2022Quote: ... or ~7.5 million cells/mL (Fig S18c) before loading into wells of preformed phantoms consisting of 1% agarose (Bio-Rad) in PBS.
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bioRxiv - Plant Biology 2022Quote: ... The resin was resuspended in 5 mL of extraction buffer and transferred to a gravity flow column (Biorad, Hercules, CA). After loading ...
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bioRxiv - Plant Biology 2022Quote: ... A total of 3 ml of Ni2+- loaded resin was packed into Econo-Pac columns (cat. Number 7321010 – Bio-Rad) and equilibrated with 3 column volumes (CV ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... detergents and undigested protein were removed using a Bio-Scale Mini Macro-Prep High S 1 mL cartridge (Bio-Rad) on an Agilent 1100 HPLC system at 1 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... Any residual BioBeads were removed by passage of the solution through a Micro Bio-Spin chromatography column (0.8 ml; Bio-Rad). The proteoliposome sample in the 2 ml Eppendorf tube was placed on ice in preparation for the lipid flipping assay.
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bioRxiv - Microbiology 2023Quote: ... Protein was produced from 300-600 ml of LB and purified on a 1ml HisTrap column connected to the NGC Chromatography System (BioRad). After lysate loading column was washed with 10CV of buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied to an equilibrated 1-ml StrepTrap HP chromatography column connected to the NGC Chromatography System (BioRad), washed with 20 CV of StrepA and elution was performed by applying a gradient concentration of StrepB (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants containing the protein extracts were transferred into fresh 1.5 ml tubes and protein concentration was measured by Bio-Rad Protein Assay Kit using BSA as protein standard (5000002 ...
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bioRxiv - Molecular Biology 2023Quote: ... The column was washed with 50 mL of wash buffer and then transferred onto a Fast protein liquid chromatography system (BioRad). The column was further washed until the UV trace was flat ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was rinsed three times for 10 minutes in TBS followed by the addition of 1 mL ECL solution (BioRad) to the membrane and visualized on the ImageQuant LAS 500.
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bioRxiv - Evolutionary Biology 2023Quote: ... Total protein (mg/mL) was determined for each sample using Bradford assay reagent (Catalogue # 5000002, Bio-Rad, Hercules, California, USA). Liver mitochondria (0.175 mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... software in 0.2 ml 96-well PCR plates (dot scientific inc.) sealed with transparent Microseal B Adhesive Sealer (Bio-Rad). Data were analyzed using the comparative Ct (2-ΔΔCt ...
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bioRxiv - Biochemistry 2023Quote: ... It was first clarified by centrifugation at 10,000 g for 30 min and loaded onto a 10-mL self-assembled Affi-Gel Blue gravity column (Bio-Rad). The column was sequentially washed with 50 mL resuspension buffer containing 0.3 M NaCl ...