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Citations for Bio-Rad :
1651 - 1700 of 4563 citations for Monobenzyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The wash was repeated and microparticles resuspended in wash buffer for analysis on a Bio-Plex®100 Luminex machine (Bio-rad).
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bioRxiv - Microbiology 2020Quote: ... or lacking (non-reducing) 100 mM β-mercaptoethanol and resolved by SDS-PAGE in a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD ...
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bioRxiv - Microbiology 2021Quote: ... protein pellets were resuspended in 100 µL of 6M urea in 50mM ammonium bicarbonate for DC® protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Cell Biology 2021Quote: ... Samples were then centrifuged (2500 × g for 5 min) and pellets were resuspended in 100 μL of PAGE Laemmli Sample Buffer (Bio-Rad, 1610747) mixed with 50 μL TE 1X pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using TRIzol and 100 ng of RNA were reverse transcribed using Quantitect followed by qPCR using iTaq SYBRgreen (Bio-rad, 1725122).
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bioRxiv - Genetics 2023Quote: ... final concentration 100 ng of cDNA was used for expression analysis with iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 1725121). qPCR was performed in a CFX96 Bio Rad reader with following cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... were loaded on 10 % or 4–20 % Mini-PROTEANR TGX™ Precast Protein Gels and separated at 100 V in Tris/Glycine/SDS buffer in Mini-Protean Tetra System (Bio-Rad). The proteins were transferred to a polyvinylidene difluoride membrane (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μg of cell lysates were separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), transferred to a PVDF membrane for analysis using anti-FLAG (Sigma Aldrich #F3165 ...
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bioRxiv - Microbiology 2023Quote: ... 20 µL reaction mixes containing 100 ng template RNA were added to a 96-well PCR plate (Bio-Rad Cat. No. MLL9601) and sealed with adhesive Microseal ‘B’ PCR Plate Sealing Film (Bio-Rad Cat ...
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bioRxiv - Cell Biology 2023Quote: 5 μg DNA/106 cells or 100 nM siRNA /106 cells were transfected by electroporation (200 mV, 950 μF and 480 Ω; Bio-Rad). Expression was measured 24-48 h post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were eluted from sepharose beads by boiling at 95 - 100 °C for 5 min with 40 µl sample buffer diluted from 4x sample buffer (Bio-Rad, 1610747) with Lysis Buffer ...
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bioRxiv - Microbiology 2023Quote: ... 100 µL of the electrocompetent cells were aliquoted into pre-chilled 0.2 cm gap Gene Pulser Electroporation Cuvettes (Bio-rad Laboratories #1652086) and kept on ice ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total protein amount of 100 μg for each sample was separated on 12% Criterion gels (Bio-Rad Laboratories, Hercules, Ca, USA), following the manufacturer’s instructions ...
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bioRxiv - Zoology 2024Quote: ... and 100 ng of RNA were used for cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA), resulting in a final concentration of 5 ng/μl for each sample.
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bioRxiv - Molecular Biology 2024Quote: ... The remaining 30 μL sample was run on a 10% acrylamide SDS-PAGE gel at 100 V for 15 minutes and stained with Coomassie (Bio-Rad #P1610786) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted protein was subjected to an additional purification step using fast protein liquid chromatography (FPLC) and a Superdex 200 Increase 10/300 GL column (Cytiva, 28990944) in Chelex 100-treated (Bio-Rad, 1422832) dialysis buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804, anti-β-Tubulin; 1:1000 dilution, Bio-Rad #12004166). Then membranes were washed with 1X TBST 3 times (10mins each wash ...
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bioRxiv - Plant Biology 2023Quote: ... followed by addition of primary antibodies at 4 °C overnight with rotation (mouse anti-ϕ3-actin [Proteintech, 66009-1-Ig], 1:1000, and rat anti-χξ-tubulin [YOL1/34, MCA78G, Bio-Rad], 1:1000). The specimen was washed three times with PBST and incubated with secondary antibodies (anti-mouse ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were anti-mouse (Bio-Rad, #170-6516, dilution 1:1000 or 1:2000) and anti-rabbit (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... 1:5000 and 1:10000 dilutions of the libraries in triplicate on iQ5 (Bio-Rad) and CFX96 Touch qPCR machines ...
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bioRxiv - Plant Biology 2021Quote: ... 0.05% SDS) and a semi dry transfer cell (Bio-Rad, 1 mA/cm2, 1 h). Membrane was blocked with protein free blocking solution T20 (Pierce ...
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bioRxiv - Microbiology 2019Quote: ... was diluted 1:5000 in 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Blots were incubated at room temperature for 2 h ...
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bioRxiv - Immunology 2020Quote: ... A total of 50μg protein was diluted 1:1 with 2x Laemmli buffer (Bio-Rad), resolved by SDS-PAGE and transferred into PVDF membranes ...
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bioRxiv - Biochemistry 2021Quote: Purified protein was mixed in 1:1 ratio with 2X laemmli loading dye (Bio-rad) prepared according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1× iTaq Universal SYBR Green Supermix or 1× iTaq Universal Probe Supermix (Bio-Rad, USA), 300 nM each of forward primer (late RT product ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with the HRP-conjugated secondary antibodies (1:2500-1:10000, Bio-Rad) for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were mixed 1:1 with 2X Laemmli Sample Buffer (Bio-Rad, Hercules, CA) (5% β-mercaptoethanol ...
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bioRxiv - Physiology 2023Quote: ... prepared at a 1:1 ratio and acquired using the ChemiDoc MP System (Bio-Rad).
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bioRxiv - Genetics 2022Quote: Samples taken from prior assays were mixed 1:1 with Laemmli Sample Buffer (Bio-Rad) with 10% 2-mercaptoethanol and vortexed to ensure adequate mixing ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with Laemmli Sample Buffer (1610737, BIO-RAD, Hercules, CA) and heated to 80°C for 30 min.
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bioRxiv - Immunology 2024Quote: Samples were prepared in 1 x Laemmli buffer with or without 1 x dithiothreitol (Biorad). Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... or BIC (20 μM) were added to the culture and cells were harvested with a 1:1 mixture of 2X Laemmeli buffer (BioRad, 1610737, 1:20 beta-mercaptoethanol) and radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...
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bioRxiv - Neuroscience 2019Quote: ... samples were prepared on ice (to a final volume of 40 microliters) in a mixture of Laemmli Sample Buffer and 2-Mercaptoethanol (BioRad) and then vortexed and denatured for 10 min at 70°C ...
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bioRxiv - Microbiology 2019Quote: ... boiled (95°C, 10 min), centrifuged (1,000 × g, 2 min) and loaded on 10% or 4-20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Plant Biology 2019Quote: ... 2% of samples were used as templates to perform quantitative PCR (qPCR) using SYBR Green PCR Master Mix (Bio-Rad) with primers recognizing the Ds element or an unrelated intergenic region in soybean chromosome 7 ...
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bioRxiv - Developmental Biology 2019Quote: ... monomeric ATP-bound 2 μM rabbit muscle actin was treated overnight at 4°C with analytical grade anion exchange resin (BioRad), hexokinase (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: ... Peak fractions from SEC purification were pooled and 2-mercaptoethanol was removed with Econo-Pac 10DG desalting column (Bio-rad) which was pre-equilibrated with buffer C ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was diluted to 10 ng/µland 2 µl was used in each RT-qPCR reaction with 10 µl iTAQ Universal SYBR green supermix (BioRad) and 400 nM primers ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto 0.2 µm pore nitrocellulose membranes for 10 min at 2.5 V using the Trans-blot turbo transfer system (Bio-Rad). For more sensitive detection of neurexin levels ...
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bioRxiv - Molecular Biology 2020Quote: ... 95°C), and run (loaded in 2 wells, 20 μL per well) on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Biochemistry 2020Quote: ... Plates were imaged together with a 1 cm2 reference area on black background (see example in Supplementary Data 2) using a Gel-Doc XR + gel documentation system (BioRad). Images were analysed in MATLAB using the code in Supplementary Note 2 to determine the growth area (in cm2 ...
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bioRxiv - Neuroscience 2021Quote: MV pellets were sonicated three times for 10 s at 20 Hz (Vibra cell VCX130) in 2% SDS and heated at 56°C in Laemmli loading buffer (Biorad). The protein content was measured using the Pierce 660 nm protein assay kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The input (15 µg) and eluted proteins (∼2% of IPT) were fractionated in 4−15 % SDS-PAGE gels (Bio-rad) and analyzed by immunoblotting ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 2 μl of five times diluted cDNA and monitored by CFX Connect Real-Time PCR Detection System (Bio-Rad). The following cycling parameters were used ...
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bioRxiv - Microbiology 2020Quote: ... at 1/10,000 dilution in TBE buffer for 2 h with rocking and visualised using a Gel Doc EZ Imager (Bio-Rad). Band intensity was quantified using ImageJ software ...
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bioRxiv - Plant Biology 2021Quote: ... Quantification of relative gene expression was performed using the comparative CT method (2-ΔΔCt) (Schmittgen & Livak, 2008) on a CFX96 Real-Time System (BioRad). Primers used are listed in Supplemental Table 1.