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Citations for Bio-Rad :
1451 - 1500 of 4847 citations for Monobenzyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The detergent was then removed by four successive additions of Bio-Beads SM-2 Resin (Bio-Rad) over the course of ∼24 h at 4 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μL of the diluted samples were used as input in a ddPCR supermix (Bio-Rad, 1863023). Primers and probes ...
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bioRxiv - Biochemistry 2024Quote: ... A hydroxyapatite column was prepared by resuspending 2 g Bio-Gel HTP Hydroxyapatite (Bio-Rad, 130-0420) in 12 ml CDC6 HTP-wash buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4-15% Tris-Glycine gel (Bio-Rad Cat#5678084) using Tris-Glycine running buffer (0.2M Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... with integration time set to 2 s and imaged using a Gel Doc XR system (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Lysis was performed by directly adding 2x Laemmli sample buffer (supplemented by 2-mercaptoethanol, Bio-Rad Laboratories) at a ratio of 1 µL per 0.1 mg of cell pellets and pipetting up and down ...
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bioRxiv - Microbiology 2023Quote: ... The relative gene expression was analysed by the comparative 2-ΔΔCt method using CFX 96 software (BioRad). Data were normalized with the endogenous control ...
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bioRxiv - Neuroscience 2023Quote: ... SARS-CoV-2 RT-qPCR reactions (10 µL) using the iTaq Universal Probe One-Step Kit (BioRad) and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713 ...
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bioRxiv - Systems Biology 2023Quote: ... Time points were measured every 2-3 days by flow cytometry analysis of >10,000 cells (BioRad ZE5). For experiment with BRM014 (MedChemExpress #HY-119374) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed in sample buffer (2× Laemmli sample buffer, No. 1610737, Bio-Rad, Hercules, California, USA). Whole-cell lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-rat TCRαβ antibody (2 µg/rat dissolved in saline solution, clone R73, Bio-Rad #MCA453GA) from day 9 to day 14 to deplete DRG T cells as previously described 66 ...
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bioRxiv - Physiology 2023Quote: ... Captured complexes were dissociated from the beads in the ×2 Laemmli buffer (Bio-Rad Laboratories, Hercules, CA) and analyzed by SDS-PAGE/western blotting using chemiluminescent substrate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining was done in 2% milk-TBST (+ 0.01% sodium azide) and detected using ChemiDoc (Biorad).
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bioRxiv - Biochemistry 2024Quote: ... Detergent removal was carried out at 4°C via incubation with Bio-Beads SM-2 (Bio-Rad) at a concentration of 200 mg ml-1 for three times with two hours for each time ...
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bioRxiv - Plant Biology 2024Quote: ... centrifuged for 2 minutes before running through 4-20% mini Protean TGX Stain Free Gel (BioRad, USA). The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore ...
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bioRxiv - Systems Biology 2024Quote: ... containing 2 μl of cDNA template was prepared and analyzed using CFX Connect Real-Time instrument (BioRad) with the following thermocycling conditions ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended to OD 10 in 1X Laemmeli Buffer supplemented with 2-mercaptoethanol (10%) (Bio-Rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with horseradish peroxidase conjugated secondary antibodies (1:5,000 or 1:10,000 in non-fat dry milk, 1 h, room temperature; Bio-Rad Laboratories). Secondary antibodies were visualized using enhanced chemiluminescence (ECL Plus ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was washed three times with T-TBS for 3 x 5 min and incubated for 1 h with 1/1000 solution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (1:1000; Biorad) and washed 3 times with T-TBS and 1 time with TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
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bioRxiv - Molecular Biology 2021Quote: ... 1:3000 or 1:10000 (for H3) dilutions of secondary antibodies (BioRad) (1 h at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... according to 1:1 by adding appropriate amount of ECL (Bio-Rad) color solution in the dark conditions and photographed using an EZ-ECL chemiluminometer Detection Kit for HRP (Biological Industries ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was diluted 1:1 with 2X Laemmli sample buffer (BioRad, 1610737) under reducing conditions and boiled for 5 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:1000 mouse anti-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360), or 1:10,000 mouse anti-actin (C4 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein sample were diluted 1:1 with 2X SDS Laemmli buffer (Biorad) and incubated for 30 mins at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... estrogen receptor (Santa Cruz sc-542, 1:20; BioRad MCA1799T, 1:25), Ltf (Bioss bs-5810 ...
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bioRxiv - Physiology 2021Quote: ... Samples were mixed 1:1 with Laemmli’s sample buffer (BioRad 161-0737) containing 10% 2-Mercaptoethanol (Sigma M3148 ...
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bioRxiv - Cell Biology 2024Quote: ... Rat anti-F4/80 (Bio-Rad, clone CI:A3-1, #MCA497GA, 1:50), Rabbit anti-CD45 (Abcam ...
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bioRxiv - Immunology 2021Quote: ... samples were transferred to FACS polystyrene tubes using a disposable bacterial filter (50 μm, Sysmex) and macrophages were sorted into 100 μL of PBS using a S3TM Cell Sorter (BioRad). Isolated cells were verified by fluorescence microscopy and differential interference contrast.
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was obtained by incubating the samples (whole embryos or adult caudal fin fragments) in TE buffer supplemented with 5% Chelex-100 (BioRad) and 10 μg/ml Proteinase K (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... and electrophoresed at 100-200 V for one to two hours in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (BIORAD). Proteins were transferred onto PVDF membranes (TransBlot Turbo Mini PVDF Transfer Packs ...
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bioRxiv - Molecular Biology 2020Quote: Whole flies or human primary cell pellet was homogenised in 2x Laemmli loading sample buffer (100 mM Tris pH 6.8, 20% glycerol, 4% SDS; Bio-Rad) containing 50 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA from 100 ng of RNA from each fractions is synthesized using iScript™ Reverse Transcription Supermix (Bio-Rad, #1708841) in a 10 μL volume with a longer incubation time of 30 mins for reverse transcription step ...
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bioRxiv - Immunology 2019Quote: ... Real-time PCR was performed using 90 well-plates with 50-100 ng of cDNA per well containing the SsoFast EvaGreen Supermix (Biorad) and gene-specific primers ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were dialyzed in HEPES sodium salt-HCl (75 mM, pH 7.0) with Chelex-100 resin (5 g/L, Bio-Rad) to remove any divalent metal contaminants ...
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bioRxiv - Bioengineering 2019Quote: ... was used for qPCR with 100 ng cDNA in a CFX96 Touch Real-Time time PCR Detection System (Bio-Rad). All reactions were performed on three biological and technical replicates ...
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bioRxiv - Biochemistry 2020Quote: ... cell lysates (50 - 100 μg) or plasma membrane fractions (80 - 100 μg) were resolved on SDS-PAGE gel and transferred to PVDF membrane (BIORAD). The membranes were blocked with 5% BSA and immunoblotted using primary anti-phospho-β2AR antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... that was analyzed using the flow-based Luminex™ 100 suspension array system (Bio-Plex 200; Bio-Rad Laboratories, Inc.).
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bioRxiv - Biochemistry 2022Quote: ... transferred to PVDF membrane (Amersham Hybond 0.2 um PVDF) at 100 V for 1hr 10 min with mini-protean tetra system (Bio-Rad) at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Intact cells were divided into 100-μl aliquots and heated individually at different temperatures for 3 minutes in a PCR machine (Biorad), followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The absorbance of 100 μl of the medium was then measured in a 96-well plate using a BenchmarkPlus microplate spectrophotometer (Biorad). The absorbance at 690 nm ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed and then precipitated in 0.1 M glycine pH 3.5 and neutralized with 0.5 M Tris?Cl and 1.5 M NaCl pH 7.4 before SDS-PAGE electrophoresis (8%, at 100 V for 90 min) followed by transfer to a PVDF membrane (BioRad). Membranes were washed in Tris-buffered saline (TBS ...
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bioRxiv - Physiology 2022Quote: ... in TBE buffer against a 100 bp DNA ladder (GeneRulerTM, Fermentas) using a Model 200/2.0 Power Supply (Bio-Rad). Gels were imaged using a Genius Bio Imaging System and GeneSnap software (Syngene).
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 57 DNA from whole mosquitoes was extracted using 200 µL of 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA) and 3 μL of Proteinase K (20 mg/ mL ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA recovery from this sample applied Chelex 100 Resin (Bio-Rad, 10% w/v in 500 μL in ddH2O) with the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Multiplexed PCR reactions using 100 ng cDNA were prepared with the ddPCR supermix for probes (No dUTP, Bio-Rad 1863024) and fractionated into >20,000 droplets using the Bio-Rad QX200 droplet generator with droplet generation oil for probes (Bio-Rad 1863005) ...
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bioRxiv - Cell Biology 2019Quote: ... Complementary DNA (cDNA) was made by converting at least 100 ng RNA with the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2020Quote: ... After loading the lysate into the packed volume of 100 µL of FLAG agarose on a gravity column (Bio-Rad) and reloading the flow through twice ...
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min at 100°C in SDS-PAGE sample buffer and separated on Tris-glycine 4-20% SDS-PAGE gels (BioRad). Detected protein bands for each blot were quantified using ImageJ software for gel densitometry measurements of NSP4:GAPDH.
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... with the main exception that the standard square curve electroporation (two pulses at 30V for 3 ms with an interval of 100 ms) was performed once or four times with an interval of 3s Gene Pulser Xcell (Biorad). Zygotes were cultured to two-cell embryos and transferred into pseudopregnant females ...