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Citations for Bio-Rad :
1751 - 1800 of 4563 citations for Monobenzyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% non-fat milk (Bio-Rad, Hercules, CA, USA) or 5% bovine serum albumin (BSA ...
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bioRxiv - Systems Biology 2023Quote: ... and total protein abundance was normalized to 2 μg using the Detergentcompatible Protein Assay (Biorad, Hercules, CA, USA, Cat. # 5000111). 2 μg α-RelA antibody (Cell Signaling ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a 0.2 µm or a low-fluorescence 0.45 µm polyvinylidene difluoride (PVDF) transfer membrane using a Trans-Blot Turbo (Bio-Rad). Following antibody incubation ...
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hour and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was generated from ∼2 ng of total RNA from each sample using iScript Reverse Transcription Supermix for qRT-PCR (170-8891; BioRad) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Protein samples were loaded on 4-15% polyacrylamide gels (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... 390 μl of cells were mixed with 10 μg of in vitro transcribed RNA and added to a 2 mm electroporation cuvette (BioRad). BHK-21 cells were electroporated with 1 pulse at 1.2 kV ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad). Duplicates of samples and plasmid standards were amplified by using the following protocol ...
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bioRxiv - Microbiology 2023Quote: ... 10 min), centrifuged (1,000 x g, 2 min) and loaded on 10% or 4–20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Cell Biology 2023Quote: ... then the pellet was resuspended in 212 µL of Laemmli sample buffer with 2-mercaptoethanol added (Bio-Rad, Hercules, CA). To neutralize the residual TCA ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was determined using the 2−ΔΔCT method129 as part of the CFX Maestro for Mac 1.0 software package (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... The mixture was allowed to stand at room temperature in the dark for 30 min before the detergent was removed by successive addition of BioBeads SM-2 (BioRad). Nanodiscs were concentrated using Vivaspin® 500 centrifugal filters (10 kDa MWCO PES membrane).
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bioRxiv - Microbiology 2023Quote: ... DD-HA-BFD2Δeif1.2 were subjected to electrophoresis using 10% polyacrylamide SDS-PAGE gels and transferred onto 0.45-µm nitrocellulose membranes (Bio-Rad) with wet/tank transfer system (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were resuspended by addition of ice-cold PBS supplemented with 2% FBS and counted using a TC-20 cell counter (BioRad). 2.5-5x105 cells were harvested ...
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bioRxiv - Neuroscience 2024Quote: ... The samples (2 μg) were loaded onto a 15% SDS-PAGE gel and transferred to PVDF membranes (0.2 μm, Bio-Rad). After blocking with 5% BSA in TBST at room temperature for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... mixed with 25 μl 1.5 mM CaCl2 and 42 μl ddH2O using 2 mm gap cuvettes with 3.5 msec/500 volt in BIORAD Gene Pulser Xcell Electroporation System (BioRad, USA). Transfected cells were immediately transferred into 5 mL prewarmed medium in 25 cm2 flasks and left to recover overnight at 26 °C ...
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bioRxiv - Microbiology 2023Quote: ... cells and plasmids in 10% glycerol were first heat-shocked at 56°C for 2 min followed by immediate electroporation in 0.1-cm cuvette (Bio-Rad) using electroporation program of 2.5 kV and time constant of 2.3-2.5 ms on the Bio-Rad Micropulser ...
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bioRxiv - Microbiology 2024Quote: ... 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad). Samples and plasmid standards were amplified using the following protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were run on 2% TBE agarose gel and post-stained with ViSafe Red Gel Stain (Vivantis Technologies) and visualised using GelDoc (BioRad). The relative intensity of the bands was quantified using ImageJ ...
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bioRxiv - Biophysics 2023Quote: ... and yeast cell growth was monitored by measuring the OD600 at 2 h increments in a microplate reader (Benchmark Plus, BioRad). The remainder of the 10 ml cultures was used to assess Pgp expression by Western blot analysis of microsomal membrane preparations using the monoclonal C219 anti-Pgp antibody.
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bioRxiv - Molecular Biology 2020Quote: ... the total protein concentration was adjusted to 1.8 mg/ml using Bradford reagent (Bio-Rad) and 1 ml aliquots were separated over a Superdex 200 16/60 equilibrated with 20 mM Tris-HCl pH 7.5 and 50 mM NaCl ...
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bioRxiv - Synthetic Biology 2019Quote: ... The nickel sepharose-lysate suspension was poured onto a 10 mL disposable column (Bio-Rad), and the settled resin washed with 20 column volumes of a first wash solution (50 mM KH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... The collected beads were poured into a 25 mL Econo-Column Chromatography Column (Bio-Rad) and washed with four column volumes of wash buffer (20 mM Tris–HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The last wash was conducted in a disposable 10 ml Poly-prep column (Bio-RAD) and the protein was eluted from the beads with elution buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... Detergent was removed from the reaction by addition of 0.1 g/mL Biobeads (Bio-Rad) and incubation at 4 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The 3 mg/mL protein sample was injected onto NGC Liquid Chromotography System (Bio-Rad) equipped with a HiPrep Sephacryl S-500 HR column equilibrated in FPLC buffer and separated into 156 ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... Sly1-bound resin was collected in a 25 mL disposable Econo-Pac column (Bio-Rad) by gravity and washed with 25 mL of SLY1 buffer supplemented with 50 mM imidazole at pH 7 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The slurry was applied to a 10-mL Poly-Prep chromatography column (Bio-Rad, USA), which was then washed with 10 mL of purification buffer B (purification buffer A with 50 mM imidazole) ...
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bioRxiv - Biochemistry 2020Quote: ... the beads was transferred to a gravity-flow Econo column (49 ml volume; Bio-Rad) and washed with 2 column volumes (CV ...
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bioRxiv - Bioengineering 2019Quote: ... generated droplets or Gelbeads were extracted into PCR tubes (0.2 mL individual PCR tubes, BioRad) and incubated in a thermal cycler (T100 ...
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bioRxiv - Biophysics 2022Quote: ... lysate-resin suspension was loaded onto a 20 ml polypropylene gravity-flow column (Bio-Rad). After collecting the flow through ...
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bioRxiv - Biophysics 2022Quote: ... lysate-resin suspension was loaded onto a 20 ml polypropylene gravity-flow column (Bio-Rad). After collecting the flow through ...
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bioRxiv - Biochemistry 2022Quote: ... Detergents were removed by the sequential addition of 60 mg/mL Biobeads SM2 (Bio-Rad) for three times within 3 h ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His6 was then purified using a pre-packed 5 ml IMAC cartridge (Bio-rad) on a Bio-rad FPLC ...
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bioRxiv - Microbiology 2022Quote: ... Membrane pellets were resuspended at 11 mg/ml (as determined by Bradford assay, Bio-Rad) in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and cell density (cells/ml) was measured by an automated cell counter (TC20, Bio-Rad). The culture was diluted by medium lacking nitrogen and carbon sources to a density of 1×106 cells/ml ...
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bioRxiv - Biophysics 2022Quote: ... UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD). VDAC1 Porin was used as a mitochondrial loading control and detected by a mouse anti-VDAC1 0.1 μL/mL (16G9E6BC4 - Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification of cell number per ml was measured on a TC20 Cell Counter (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA) and 4 ml 1:6 diluted cDNA template along with 200 nM forward and reverse primers in a total volume of 10 ml per reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... with 0.2 ml Flat PCR Tube 8-Cap Strips (Bio-Rad™, optical, ultraclear, #TCS0803). Optimum amplification conditions were determined for each by amplifying six concentrations of a 5× dilution series of MG1655 pJK14-Tn4rev pZA31-mCherry-tnpB using two-step amplification with a thermal gradient of 55–75°C ...
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bioRxiv - Physiology 2023Quote: ... Matching slides per animal were immunofluorescence (IF) stained for cd68 (5 µg/mL, MCA1957, BioRad) followed by donkey anti-rat CF750 (2 µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... before rinsing quickly with 2x10 mL TBS before treating with clarity ECL substrate (Bio-Rad). Signals were detected using a Bio-Rad ChemiDoc Imaging System ...
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bioRxiv - Microbiology 2023Quote: ... Protein-bound resin was then applied to a 10-mL chromatography column (BioRad, Hercules, CA) and washed twice with 10 mL wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluent was then applied to a 10-mL self-assembled HAP column (Bio-Rad), washed with 50 mL resuspension buffer ...
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bioRxiv - Microbiology 2024Quote: ... at 4 °C for 30 min in a 10 ml poly-prep column (Bio-Rad). The column was then stabilized for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories, Hercules, CA). Separated proteins were transferred onto nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... Suspension array analyte concentrations were determined using a Bio-Rad Bio-Plex System Luminex 100 and Bio-Plex manager 5.0 (Bio-Rad Laboratories, Hercules, CA)40 ...
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bioRxiv - Bioengineering 2021Quote: ... The obtained DNA extract (100 μL) was stored at −20 °C until further investigation and subjected to qPCR analysis using 96-well plates (Bio-Rad, Hercules, USA), the Thermocycler CFX connect system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled at 100°C for 5 min prior to SDS-PAGE separation on 12% acrylamide Mini-protean TGX precast gels (Bio-Rad Laboratories, US) and Western blot analysis.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A total of 100 μg brain lysate was run on a Bio-Rad Mini-Protean TGX 4-20% polyacrylamide gel (4561094, Bio-Rad, Hercules, CA) at 200V for approximately 40 minutes ...