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Citations for Bio-Rad :
1601 - 1650 of 4847 citations for Monobenzyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 25 μL of lysate mixed with sample buffer (1:1 ratio) (Bio-Rad) was loaded per lane in a 10% SDS-PAGE gel (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... and rat anti-F4/80 (1:500, Clone A3-1, MCA497GA, Biorad, USA). After 3×5 min washes ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... Phalloïdine 647N ATTO (1/200) and DAPI nuclear dye (Bio-rad,1/1000) were diluted in the blocking solution ...
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bioRxiv - Microbiology 2022Quote: ... and mixed 1:1 with 2x Laemmli Buffer (Bio-Rad Laboratories, 161-0737). 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were mixed 1:1 with 2X Laemmli sample buffer (Bio-Rad) with 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and rabbit anti-alpha actinin-1 at 1:1000 (Bio-Rad Laboratories #VPA00889). Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was decanted and the cell pellet was washed twice with cold cPBS (PBS treated with Chelex-100 resin (Bio-Rad) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was then added and the solution was mixed at 4°C for 10 minutes before addition of 100 mg of Biobeads SM2 (Bio-Rad). Biobeads were washed into methanol ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 9.0 running buffer and visualized using the FITC channel set at 100 µM resolution in low intensity mode on a GelDoc Pharos imager (BioRad Laboratories). ImageQuant software was used to analyse the fraction of probe bound in each lane and GraphPad Prism version 8 software was used to calculate the Kd ...
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bioRxiv - Molecular Biology 2020Quote: ... 100-500 ng of total RNA was reverse-transcribed into complementary DNA (cDNA) by using iScript™ Reverse Transcription Supermix (Biorad). Quantitative real-time PCR was performed using iTAQ™ SYBR Green PCR Kit (Biorad ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 μg of total RNA sample (100 ng/μl) was retrotranscribed in stable cDNA with iScriptTM Advanced cDNA Synthesis Kit (Bio-Rad). Genes of interest were amplified with Itaq UniversSYBR Green (Bio-Rad) ...
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bioRxiv - Biophysics 2019Quote: ... Temperature was increased at a rate of 1°C per minute from 4°C to 100°C and fluorescence was measured every minute with a qPCR thermocycler in FRET mode (Bio-Rad). Aggregation temperature was determined by locating the global minimum of the second derivative of the raw data ...
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bioRxiv - Physiology 2019Quote: ... in TBE buffer against a 100 bp DNA ladder (GeneRuler™, Fermentas) using a Model 200/2.0 Power Supply (Bio-Rad). Gels were imaged using a Genius Bio Imaging System and GeneSnap software (Syngene).
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was generated from 100-300 ng of total RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad). cDNA was diluted 2X with water ...
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bioRxiv - Cell Biology 2019Quote: ... Intact cells were divided into 100-μl aliquots and heated individually at different temperatures for 3 minutes in a PCR machine (Thermal cycler, BIO-RAD), followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... All washes were done with 100 µl PBST (PBS with 0.05% Tween 20) using a handheld magnetic washer (Bio-Rad #171020100). The incubation time on the magnet was always 2 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sample (100 μL) was analyzed using an Agilent 1200 Series HPLC instrument equipped with an Aminex HPX-87H column (Bio-Rad). Water containing 5 mM sulphuric acid was used as the mobile phase ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 µg of total protein was loaded onto 8 to 15 % SDS-PAGE minigels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... Between 50 and 100 µg of total protein was loaded onto a gradient (4-15%) polyacrylamide gel (BioRad, Hercules, CA, USA). Proteins were then transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2021Quote: ... gel containing 8 M urea (48% (w/v)) and 1X TBE was prerun for 30 min at 100 V in a vertical mini- PROTEAN tank (Bio-Rad). Gels were flushed and loaded with 10 μg of total RNA in 2X TBE/Urea sample buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... 100 µg of protein were loaded per lane and electrophoresed on a 10% TGX precast SDS PAGE gel from Bio-Rad. Protein was transferred onto a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: A total of 100 prophase-I arrested oocytes were pooled and mixed with Laemmli sample buffer (Bio-Rad, cat #161-0737) and denatured at 95°C for 10 min ...
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bioRxiv - Bioengineering 2022Quote: Samples were washed in PBS and fixed in 3% paraformaldehyde (PFA) for 15 minutes at room temperature (RT) and permeabilized by 0.5% Triton x-100 (Bio-Rad, USA) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: 100 µL of well mixed anti-FLAG M2 Affinity Gel aliquots were loaded on columns (Micro Bio-Spin Columns, Bio-Rad) and washed two times with 1 mL of cell lysis buffer by gravity flow ...
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bioRxiv - Cell Biology 2022Quote: ... and run at room temperature with 100 V (constant) before being transferred to polyvinylidene fluoride or nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad) for 10 minutes at 2.5 A ...
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bioRxiv - Genetics 2019Quote: ... Oil Red O stain was extracted with 100% isopropanol and the absorbance was measured at 492 nm by a multi-well spectrophotometer (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... the cells were divided into 100 μl aliquots and heated individually at different temperatures for 3 minutes (Thermal cycler, BIO-RAD) followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Each gradient was then separated into several fractions (i.e., bottom-to-top) of equal volumes (about 100 μl) using a fraction collector (BioRad, Hercules, USA). RNA from all fractions was prepared following standard TRI Reagent® protocols (Molecular Research Center Inc. ...
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bioRxiv - Genetics 2021Quote: ... according to the instructions of the manufacturer or extracted using Chelex® 100 resin (Bio-Rad Laboratories Inc., Hercules, CA USA). Two final elutions of DNA were made from the kit extractions with the first being used for construction of genomic libraries for a related study and the second being used for screening of Vssc mutations after being diluted 1:5 with water ...
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bioRxiv - Neuroscience 2020Quote: ... The beads were then analyzed using flow-based Luminex™ 100 suspension array system (Bio-Plex 200; Bio-Rad Laboratories, Inc.). Standard curves were generated by Bio-plex Manager software to determine unknown sample concentration ...
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bioRxiv - Neuroscience 2020Quote: ... The bead sets were analyzed using a flow-based Luminex™ 100 suspension array system (Bio-Plex 200; Bio-Rad Laboratories). A five-parameter model was used to calculate final concentrations ...
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bioRxiv - Microbiology 2020Quote: ... whole cell lysates were incubated at 100°C for 10 minutes in 50 μl SDS loading buffer (950 μl Bio-Rad® Laemmli buffer ...
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bioRxiv - Microbiology 2021Quote: ... The protein contents of 100-µl cell samples were measured using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). Uptake rates were calculated from retained activity and total added activity and expressed in pmol amino acid µg-1 of protein.
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bioRxiv - Immunology 2022Quote: ... 10 mM sodium bicarbonate in iron-free HEPES-buffered saline (HEPES 20 mM, NaCl 150 mM, treated with Chelex-100 chelating resin [Bio-Rad, Hercules ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Plant Biology 2022Quote: ... Infiltrated leaves were infiltrated with 100 µM D-luciferin and LUC activity was quantified after 48 h using a low-light CCD imaging apparatus (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Microbiology 2024Quote: ... 80µl of ice-cold bacterial cells were mixed with 1µl plasmid (pBAD28 == pEMPTY or pHND10 == pAPY, 100-200ng, key resources table) in chilled 0.1cm cuvettes (Bio-Rad, cat# 1652089) and immediately electroporated (Voltage = 1.8kV ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extractions containing 5 µg of Chl with 1 x SDS loading buffer were boiled at 100℃ for 5 min and loaded onto a 4 - 20% polyacrylamide gel (Mini Protean TGX, Biorad Laboratories). Proteins were transferred to a PVDF-FL membrane on a Biorad semidry blotting system ...
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bioRxiv - Microbiology 2023Quote: ... qPCRs were performed on 1 μl diluted cDNAs in 10 μl scale in technical duplicates amplifying regions of 100 – 150 bp with the iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions using primers indicated in Table 8 ...
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bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was set up in a 20-μl volume with 100 - 200 ng of RNA and was conducted using the CFX Connect Real-Time PCR System (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... an equal number of cells were lysed by resuspension in an equal volume (100 μL per 1x106 cells) of hot Laemmli buffer (Bio-Rad). If cells could not be counted upon collection ...
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was amplified using KAPA HiFi Hotstart ReadyMix PCR kit (Nippon Genetics) with T-100 Thermal Cycler (Biorad, Hercules, CA). The temperature profile of the reaction was 95 °C for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... Lactose and salts were removed by gel filtration chromatography over a BioRad P2 column (100 cm x 316 mm, Bio-Rad) using a semi-automated fast protein liquid chromatography (FPLC ...
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bioRxiv - Cell Biology 2022Quote: ... 5% sorbitol synchronized ring stage Pf NF54 parasites at 5-8% parasitemia were electroporated with 100 µg of pFC-LINUP plasmid at 0.31 kV and 950 µF using a BioRad Gene Pulser (BioRad, Hercules, CA). Positive selection with 8 nM WR99210 (WR ...