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Citations for Bio-Rad :
1501 - 1550 of 1612 citations for IL 15RA&IL 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Experiments were run using 26-well gels (Criterion TGX Precast Gels, 15 μl, 1.0 mm, 4-20%, Bio-Rad Laboratories, Inc. Cat No 5671095). 13 wells were loaded by one researcher and the other set of 13 wells were loaded by another researcher for each set of experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... Reducing SDS sample buffer was added and proteins resolved on 4%-15% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad, California, United States) or 3%-8% NuPAGE Tris-Acetate Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... and equal amounts of total protein (15 or 20 μg) were loaded in different wells on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad, Australia). A stain-free system (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... and purity assessed by 15% SDS-PAGE using Bio Rad Precision Plus Protein All Blue Standards (10-250 kDa) as molecular weight ladder (Bio-Rad; Hercules, CA, USA).
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bioRxiv - Plant Biology 2020Quote: ... Proteins extracts were boiled at 95°C and then separated onto Mini-PROTEAN ® TGX™ precast protein SDS-PAGE (4-15% gradient) gels (BIO-RAD, #4561083). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2019Quote: ... Protein extracts were mixed with loading buffer containing SDS and dithiothreitol (DTT) and loaded onto Mini-PROTEAN® TGX™ Precast Gels (4-15% gradient; Bio-Rad 4561084) in Tris-Glycine buffer ...
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bioRxiv - Cancer Biology 2021Quote: Westerns were performed by loading 30-50ug of total protein per well into 15 well polyacrylamide gels (Bio-Rad, California, USA, Cat #456-1086) and run for 30min at 200V ...
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bioRxiv - Biophysics 2020Quote: ... Pure ClyA-AS type-I (12-mer) nanopores were obtained using native PAGE on a 4-15% gradient gel (Bio-Rad, Veenendaal, The Netherlands) and subsequent excision of the correct oligomer band.
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bioRxiv - Neuroscience 2021Quote: ... total protein was extracted and 15-20µg from each sample was separated on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Biorad, Hercules, CA., USA) adjacent to the Li-COR Chameleon ladder (Li-Cor ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins from recovered fractions were analysed by Western blotting (4-15% Mini PROTEAN® TGX Stain-Free™ Gels & Trans-blot® Turbo™ Nitrocellulose Transfer Pack, BioRad). The bait proteins were probed using a His Tag HRP-conjugated antibody (Ozyme ...
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bioRxiv - Cell Biology 2019Quote: ... Resulting cDNA is used in PCR reactions (15 μl) performed in 96-well format in triplicate contained 1X SYBR green reagent (BioRad iQ SYBR Green Supermix), 0.4 μM gene specific primers and 0.5 μl of first strand reaction product (diluted 1:2 ...
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bioRxiv - Genetics 2020Quote: ... diluted samples were boiled for 5min at 95 °C and subsequently separated on a 4-15% Criterion™ TGX Stain-Free™ Protein Gel (Bio-Rad Laboratories) and ran in a Criterion™ Cell geltank (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad Mini-PROTEAN® TGX™ 4-15% Gel) according to manufacturer’s protocols for electrophoresis at 110 V for 70 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 to 30 μg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Immunology 2021Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-Rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... the indicated ESA supernatant was boiled in SDS-PAGE sample buffer and 3 μg of each sample was run with a 4-15% TGX gradient gel (Bio-Rad cat. no. 4561086), then transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2022Quote: ... 30μg protein for iPSC-derived optic cups or 15μg for iPSC-LESCs were loaded onto 4-15% Mini-PROTEAN ® TGX ™ gels (BioRad Inc., CA, USA) and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc. ...
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bioRxiv - Developmental Biology 2023Quote: Samples were run on 10% or 4-15% TGX pre-cast gels with 10 wells with 50 μL well volume (Bio-Rad, #4561034 and #4561084). Proteins were transferred overnight onto PVDF membrane (wetted briefly with methanol ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 20 μg of protein extracts were loaded per well in 4-15% Mini-PROTEAN® TGXTM Precast Protein gels (Bio-Rad cat# 4561084). After electrophoresis ...
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bioRxiv - Physiology 2023Quote: ... 5 μg of protein homogenate was separated on Criterion™ XT Bis-Tris (RyR, SERCA, Actin) or 4–15% Mini-PROTEAN® TGX™ Precast stain-free (CaMKIIδ) gels (Bio-rad) and transferred at 4°C for 16 hrs at 30 V onto 0.45 µm supported nitrocellulose membrane (Bio-rad) ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting proteins were separated by polyacrylamide gel electrophoresis (4%–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, Bio-Rad, California, USA) and then transferred to a nitrocellulose membrane (Reference ...
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bioRxiv - Genetics 2023Quote: ... The purity of viral preparations was determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis on a 4– 15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Hercules, CA, USA) in Tris-glycine- SDS buffer (Figure S3).
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bioRxiv - Developmental Biology 2024Quote: Protein lysates (40 µg per lane) from uteri or oviducts were loaded on a 4-15% SDS-PAGE gel (Bio-Rad, Hercules, CA, USA), separated in 1X Tris-Glycine Buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: Approximately 15 μg of synaptosome protein was separated on a precast gradient gel (4-15% Mini-PROTEAN® TGX™ Precast Protein Gels, 4561086, BioRad, United Kingdom) and transferred to nitrocellulose membrane (Amersham™ ProtranÒ Western Blotting Membrane ...
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cytokine levels were measured using the magnetic bead-based Bio-Plex Pro Human cytokine 27-Plex (Bio-Rad) according to manufacturer’s instructions and measured the cytokines using the Bio-Plex 200 instrument ...
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bioRxiv - Microbiology 2023Quote: ... The samples were analyzed in duplicates using the Bio-Plex Pro™ Human Cytokine panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Twenty-fold concentrated viral stocks or purified rgp120 were separated by SDS-PAGE under reducing conditions using a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc. Hercules, CA), and then transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified at 13,200 rpm for 15 min at 4°C prior to quantification by Lowry assay (Bio-Rad cat # 5000113 and cat # 5000114). Whole cell lysates were loaded into 4-20% Criterion TGX Precast 18 well gels (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 h at room temperature in HRP conjugated IgG anti-rabbit secondary antibody (Bio-Rad 170-65-15, 1:3000) or Cy5 conjugated IgG anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories Europe Ltd. ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of whole-cells and tissue extract (20-60 µg) were separated on 15% SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Mississauga, ON, Canada). The membranes were first blocked with 5% milk [w/v] in TBS/0.05% Tween 20 [v/v] for 60 min at room temperature and subsequently blotted overnight at 4º C with primary antibodies (see Table 2) ...
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bioRxiv - Microbiology 2019Quote: ... Sample preparations were run on a 15% separating gel with a 4% stacking gel under reducing conditions using Tris-glycine running buffer (mini-PROTEAN system, Bio-Rad Laboratories, Hercules, CA, USA). Silver staining using sodium thiosulphate sensitisation and silver nitrate was performed according to established methods (22).
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE (4-15% acrylamide, 150V) and transferred to PVDF membranes using the Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories, CA). Membranes were incubated for 1 hr in 5% milk (in TBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels, Bio-Rad, Hercules, USA) and transferred onto a membrane (nitrocellulose or PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels, Bio-Rad Laboratories, CA) and transferred onto PVDF membranes (Amersham Hybond ...
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bioRxiv - Biophysics 2022Quote: ... from Sigma-Aldrich (St. Louis, MO, US). Goat anti-human IgA (IgG, Catalog No. STAR141) was purchased from BIO-RAD (Hercules, CA, US). Human colostrum IgA (Catalog No ...
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bioRxiv - Immunology 2022Quote: ... Primary antibody incubation was performed by incubating slides with diluted monoclonal mouse α-human pan-cytokeratin antibody (Bio-Rad MCA1907T) overnight at 4 °C followed by secondary antibody incubation with HRP-labeled α-mouse antibody for 30 minutes RT.
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bioRxiv - Immunology 2021Quote: Multiplexed cytokine assays were performed according to the manufacturer instructions by using a Bio-Plex Pro Human cytokine screening panel (Biorad) containing 48 human cytokines ...
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bioRxiv - Cancer Biology 2021Quote: ... 48 cytokines were quantified in the cell supernatant using a Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad, #12007283) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: 17 cytokines secreted from the HCCoC were analyzed using Bio-Plex Pro Human Cytokine Group-I Panel (M5000031YV, BioRad, CA). HCCoCs were exposed to physiological WSS for 6 hours after completion of the preconditioning protocol ...
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bioRxiv - Microbiology 2020Quote: The assay was validated through a combined effort of six veterinary or human diagnostic laboratories by using the following qPCR platform and chemistry combinations: i) CFX 96 (BioRad) and LC480 I (Roche)-QuantiNova Probe RT-PCR Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatant was collected and analyzed by Bio-Plex ProTM Human Cytokine Th1/Th2 Assay according to the manufacturer’s instructions (Bio-Rad). All tests were performed in duplicates ...