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Citations for Bio-Rad :
1401 - 1450 of 1720 citations for IL 15RA&IL 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 7µg of total protein was incubated in loading buffer as described above and loaded into AnykD Mini-PROTEAN TGX Precast Protein Gels (15 wells, 15uL; Bio-Rad). Electrophoresis and protein transfer were performed as above ...
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bioRxiv - Cell Biology 2024Quote: ... clarified at 20,000 g for 2 minutes and 15 µL was loaded onto a 4-20% tris-glycine SDS-PAGE gel (Biorad #5671094). Proteins were then transferred to nitrocellulose (Sigma #10600001 ...
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bioRxiv - Cancer Biology 2024Quote: ... submerged in blocking buffer (40% ethanol and 10% acetic acid in distilled water) with shaking for 15 min and stained with coomassie blue dye (Bio-Rad) by gentle shaking overnight ...
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Self-assembled DNA-collagen bioactive scaffolds promote cellular uptake and neuronal differentiationbioRxiv - Bioengineering 2024Quote: ... The macrostructure was assembled by heating the primers at 95 °C for 30 min and then cooling them to 5 °C with a step decrease of 5 °C every 15 min using a PCR instrument (BioRad, USA). The resulting macrostructure was then stored at 4 °C until required ...
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bioRxiv - Bioengineering 2024Quote: ... SDS-PAGE was performed using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (#4561084, Bio-Rad Laboratories, Inc, USA). Before and after the laser exposure ...
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bioRxiv - Physiology 2024Quote: ... Blotted bands were detected using HRP conjugated secondary antibodies goat anti-rabbit conjugated with HRP (#1706515, Bio-Rad, 1:15, 000). ImageJ was used to calculate the fluorescence density of each band ...
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bioRxiv - Immunology 2024Quote: ... and 10 µL of this suspension was loaded into a well of a 15-well polyacrylamide gel of the Mini-PROTEAN II electrophoresis apparatus (Bio-Rad). The percentage of polyacrylamide was 10% for the separating gel and 4% for the stacking gel.
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bioRxiv - Neuroscience 2024Quote: ... Samples were heated to 95 °C for 5 min and separated on 15% TBE with urea polyacrilamide gel (BioRad Cat# 3450093) at 200 V for 60 min ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad) and transferred to a nitrocellulose membrane using TurboBlot (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.02% SDS (w/v)] for 15 min and transferred at 100 V onto a nitrocellulose membrane in a liquid transfer system (MiniProtean, Bio-Rad). Membranes were blocked in Tris-buffered saline solution (50 mM Tris pH 7.6 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured for 10 min at 95°C and then 10 µl were loaded onto a precast 4-15% protein gel (Bio-Rad). Electrophoresis was performed by applying a constant voltage of 200 V in SDS running buffer (Tris/Glycine/SDS ...
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bioRxiv - Biochemistry 2024Quote: ... using gels with a concentration of 8%, 10%, 14% or 15% acrylamide (Carl Roth, 3029.1) in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell system (Bio-Rad, 1658029FC) with running buffer containing 0.2 M glycine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083). After blocking step with 10% non-fat milk for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 15μg of protein was loaded for each sample in precast 4-15% Mini-Protean TGX Stain-Free 30μl 10-well polyacrylamide gels (Bio-Rad #456803) along with Kaleidoscop protein ladder (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μL of whole tissue lysate preps) were applied to 4−15% SDS-polyacrylamide gels (Bio-Rad; Hercules, CA, USA) and subjected to electrophoresis at 180 volts for 50 minutes in a preformulated 1× SDS-PAGE buffer (VWR) ...
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bioRxiv - Molecular Biology 2024Quote: ... was added to all samples and the products were separated on 15% polyacrylamide denaturing urea gels (19:1 acrylamide-bisacrylamide, Bio-Rad). The gels were fixed in fixing solution (40% methanol ...
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bioRxiv - Immunology 2021Quote: ... followed by detection with mouse anti-human secondary antibody conjugated to HRP (1721050, Bio-Rad Laboratories). HRP binding was determined based on reaction with TMB substrate (34028 ...
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bioRxiv - Immunology 2020Quote: Supernatants were thawed and immediately analyzed with Bio-plex human cytokine assays (Bio-Rad Laboratories, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Immunology 2024Quote: ... 17 human cytokines were analysed simultaneously using the kit and run on the Luminex analyser (BIORAD), standard curves for each cytokine created and per cytokine concentrations calculated using the BioRad software.
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bioRxiv - Immunology 2024Quote: Cytokines were measured in cell culture supernatants using luminex analysis (Biorad human 17-plex Bioplex kit) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into K562s using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured protein extracts were resolved using 4-9% SDS-PAGE (4–15% Mini-PROTEAN® TGX™ Precast Protein Gelscat# 4561083, Biorad) and then transferred to a nitrocellulose membrane.
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bioRxiv - Molecular Biology 2019Quote: ... Denatured proteins (20-40 µg) were electrophoresed in 9% SDS-PAGE gels or MiniPROTEAN® TGX™ 4-15% Precast gels (BIORAD), transferred onto a nitrocellulose membrane and probed with specific antibodies ...
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bioRxiv - Neuroscience 2019Quote: Purified CXCL12 and CXCL12 Ch/DS NC were resolved by 15% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (Bio-Rad, Hercules, USA) and electro transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2019Quote: ... Optical density was measured at a wavelength of 450 nm for 15 min with a densitometer (Microelisa Stripplate Reader, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2019Quote: ... 40µg of protein from each sample was loaded onto the 4– 15% Mini-PROTEAN® TGX™ Gel (BioRad, Catalog number: 4561083) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Equal volumes of protein were loaded onto pre-cast BioRad Mini-Protean TGX precast Gel 4-20% (15-wells BioRad cat# 4568096). The gels were run according to the manufacturer’s guidelines (110Volts for 3 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of proteins were separated by denaturing electrophoresis on a 4– 15% Criterion™ TGX™ Precast Midi Protein Gel (Biorad) and then electrotransferred to nitrocellulose membranes using the Trans-blot Turbo Transfer System (Biorad) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of the cleared lysate were separated by SDS-PAGE using 4%-15% Mini-PROTEAN TGX Precast Protein gels (Bio-Rad, #4561085) and blotted onto a Amersham Protran Premium Western blotting nitrocellulose membrane (Sigma ...
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bioRxiv - Plant Biology 2021Quote: Protein bands were run in a 15% polyacrylamide gel and transferred to a PVDF membrane using a mini trans-blot cell (Bio-Rad, USA). The membrane was stained with Coomassie blue R-250 and the bands were cut with a sterilized scalpel ...
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bioRxiv - Cell Biology 2020Quote: ... a part of each sample was run on a 4-15% Criterion TGX Stain-Free Protein Gel (26 wells, Bio-Rad, #5678085) in 1x SDS running buffer at 200 V for 32 min ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were loaded on 10% or 4-15% sodium dodecyl sulfate polyacrylamide gels (Fast Cast TGX Stain-Free or 4-15% Mini-Protean TGX Stain-Free gels, BioRad, Hercules, CA). Blotting was done on PVDF membranes using a BioRad Transblot system ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein per sample were loaded and separated using SDS-PAGE on 4-15% gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 4°C for 15 minutes at 16,000 g and protein concentration was measured by the Bradford method (Bio-Rad, Hercules, USA). Next ...
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bioRxiv - Microbiology 2021Quote: ... All qPCR amplifications were performed in duplicate and each contained 15 µL total volume of 1 × SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA), 0.3 µM of each primer ...
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bioRxiv - Microbiology 2022Quote: ... or in-house prepared 15% polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories). The membranes were incubated in blocking buffer [5% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: 15 µg of nuclear extract in SDS loading buffer or 30 µg of total protein extract were loaded on a 4-15% precast polyacrylamide gel (Bio-Rad #4561083EDU) and Color-coded Prestained Protein Marker ...
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE of the re-oxidized samples was performed on a 4-15% Criterion TGX precast midi protein gel (Bio-Rad, 5671085) under non-reducing conditions ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 μg of total protein was loaded onto 8 to 15 % SDS-PAGE mini gels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then boiled at 95°C for 10 mins and resolved in 4-15 % Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) for immunoblot analysis ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Immunology 2020Quote: ... 40 cycles of 15 s 95°C and 1 min 60°C) was carried out using a CFX 96 (BioRad, Hercules, USA) qPCR machine ...
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bioRxiv - Neuroscience 2022Quote: ... 40 mg of total protein was loaded onto a 12-15% polyacrylamide gradient gel (Mini-PROTEAN TGX Precast Mini Gel; Bio-Rad Laboratories) and transferred to a nitrocellulose membrane following separation ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amount of protein from different samples and 5-μl of Odyssey One-Color Protein Molecular Weight Marker (LiCore Cat# 928-40000) were resolved on 4—15% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE, Biorad Cat#4561086) then the protein was transferred to a nitrocellulose membrane at 4 °C overnight at 30 volts ...
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bioRxiv - Biophysics 2019Quote: ... was utilized to monitor the elution of the proteins and fractions were analyzed after separation on 4– 15% precast gradient TGX Stain-FreeTM gels (Bio-Rad Laboratories).
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bioRxiv - Genomics 2019Quote: The whole genome amplification was monitored in real time by detection of SYTO13 fluorescence every 15 minutes for 16 h using a Chromo4 real-time PCR instrument (Bio-Rad, USA) or a FLUOstar®Omega plate reader (BMG Labtech ...
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bioRxiv - Physiology 2019Quote: ... PCR reactions (15 μL) were carried out as previously described using a CFX Connect Real-Time PCR System (Bio-Rad, Hercules, CA) (Brady et al. ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... using 7.5 % polyacrylamide gels for Rad53 detection or a gradient of 4-15 % polyacrylamide for all other proteins in Mini-PROTEAN® electrophoresis chambers (Bio-Rad). Proteins were transferred to 0.45 µm pore size nitrocellulose membranes (GE Healthcare ...