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Citations for Bio-Rad :
1201 - 1250 of 1612 citations for IL 15RA&IL 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed three times with PBS-T (15 min, RT) and chemiluminescence was detected after 10 min incubation with freshly prepared Clarity Western ECL Substrate (Bio-Rad) with a LAS-4000 gel scanning station (Fujitsu Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg proteins were loaded on 4-15% Mini-PROTEAN® TGX Precast Protein Gels Stain Free™ (Bio-Rad®) for separation (200 V ...
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bioRxiv - Biophysics 2020Quote: ... Inputs (2 μl) and elutions (10 μl) samples were analyzed by 4-15% SDS-PAGE (MINI-PROTEAN TGX stain-free, Bio-Rad) and staining with Quick Coomassie (Generon ...
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... All qPCR reactions were run for 94°C for 2 min followed by 94°C for 15 s and 60°C for 1 min in a BioRad CFX384 Real Time System (Biorad, USA) qPCR machine ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 µg of total protein was loaded onto 8 to 15 % SDS-PAGE minigels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Microbiology 2020Quote: ... or the eluates were separated on a 4-15% gradient sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pre-casted gel (Bio-Rad). For Mn2+-Phos-tag SDS-PAGE gel ...
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bioRxiv - Physiology 2021Quote: ... Protein aliquots were denatured in Laemmli sample buffer at 100°C for 5 min and then loaded (10– 15 μg protein per lane) on 4–20% Criterion TGX Stain-free gels (Bio-Rad) and run for 45 min at 200 V ...
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bioRxiv - Physiology 2020Quote: ... 15 μg of protein was separated on a 10% Criterion® TGX™ Precast Protein Gel (BioRad Laboratories, 56671034, Hercules, CA) and transferred to a nitrocellulose membrane using a Trans-Blot TurboTransfer System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2021Quote: ... Then the samples were treated at 60oC for 1 h and then enzyme deactivated 95oC for 15 min in a thermocycler (Bio-rad). After the treatment ...
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bioRxiv - Microbiology 2020Quote: ... The proteins of an 0.1 OD600 equivalent quantity of cells were separated by SDS-polyacrylamide denaturing gel electrophoresis using precast TGX 4-15% gradient gels (Bio-Rad). The proteins were then transferred to a 0,2µm nitrocellulose membrane using Trans-Blot Turbo RTA Transfer Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Ref: 4568085) at 160V ...
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bioRxiv - Cancer Biology 2020Quote: ... Between 50 and 100 µg of total protein was loaded onto a gradient (4-15%) polyacrylamide gel (BioRad, Hercules, CA, USA). Proteins were then transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected after centrifugation at 15,000 ×g for 15 min at 4°C and applied to a 20 mL open column (Poly-Prep Chromatography Columns; BIO-RAD) containing 500 µL of Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: Whole-cell extracts obtained from 2·105 cells for HEK293 and 5·105 cells for Hela and A549 at 15 h post-transfection (p.t.) were separated by SDS-PAGE in 12% TGX Stain-Free gel (Bio-Rad, USA). After electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... Equivalent total protein amounts from BH and EVs were separated on 4−15% stain-free pre-cast SDS-PAGE gradient gels (Bio-Rad) under non-reducing conditions and transferred onto PVDF membranes (Sigma Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... The DDM/CHS detergent was then removed by overnight incubation with Bio-Beads SM-2 (15 mg per 1 ml protein/detergent/amphipols mixture, Bio-Rad) at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... ICP1’s genome was amplified with primers Zac68/69 and PLE replication with primers Zac14/15 using iQ SYBR Green Supermix (Bio-Rad) and the CFX Connect Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on 12% or 15 % SDS-PAGE gels and transferred onto PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad). Blocking was performed with 3% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... then 40 cycles of 15 seconds at 95°C and 60 seconds at 60°C using CFX96 Touch Real-Time PCR Detection System (Bio-rad). A standard curve was derived from the serial dilutions of DNA template following concentrations ...
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bioRxiv - Cell Biology 2022Quote: ... 192 mM glycine and 20% of methanol) for 15 min before assembly in the Mini Trans-Blot transfer cell (BioRad 1703935). The transfer was done at 4°C for 16 h at 110 mA constant current ...
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bioRxiv - Bioengineering 2022Quote: Samples were washed in PBS and fixed in 3% paraformaldehyde (PFA) for 15 minutes at room temperature (RT) and permeabilized by 0.5% Triton x-100 (Bio-Rad, USA) for 10 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Small RNAs were obtained by gel size selection from total RNA using 15% Mini-Protean TBE-Urea Gel (Bio-Rad, #4566056). The gel was run at 300V for 50 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were washed three times with T-TBS for 15 min and incubated with goat anti-rabbit HRP-conjugated secondary antibodies (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were run using MES [2-(N-morpholino)ethane sulfonic acid] running buffer at 150-160 V (110 V for the first 15 min) and then were electro-transferred to nitrocellulose membrane (Bio-Rad), submerged in transfer buffer (20% methanol ...
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bioRxiv - Molecular Biology 2020Quote: Samples were separated using 10-15% SDS-PAGE gels or 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad, 5678095). Gels were run at 100 V using 1 X SDS-PAGE Running Buffer [100 mL of 10 × 1 L Stock [30.2 g Tris Base (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Enriched CCV fractions were resolved via 1D SDS-PAGE on a 4-15% Tris-HCl gradient gel (BioRad cat# 161-1158) at a constant 200V for ∼90 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Each cDNA sample was diluted 1:15 and used directly for qPCR analysis using specific primers and the iTaq Universal SYBR green mastermix (Bio-Rad). Primers for qPCR were designed using the Primer3 software44 ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... then separated via SDS-PAGE and transferred onto a PVDF membrane (18 min, 15 V) by Trans-Blot® SD semi-dry Western blot (BioRad). The membrane was exposed as described before overnight to a PhosphoImager screen ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty micrograms of each sample were mix with sample buffer loaded and separated using Mini-PROTEAN TGX 4-15% polyacrylamide gels (BioRad: 4561083) and Tris/Glycine/SDS buffer (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... 10µg of protein per sample was loaded into 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and run at 105V for 90 minutes ...
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bioRxiv - Microbiology 2022Quote: ... added to a 15 μL reaction mixture containing 500 nM primers (sequences in Table 1) and iTaq Universal SYBR Green Supermix (Biorad, # 1725124) using a thermocycler (Mastercycler ep realplex2 ...
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bioRxiv - Pathology 2020Quote: ... proteins were subjected to primary antibody recognition using an anti-P1 antibody raised in rabbit (Siré et al, 2008) and purified by Affi-Gel 15 immnunoaffinity chromatography (BIO-RAD) using the recombinant P1 protein as the ligand (used at a 1:1000 dilution) ...
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bioRxiv - Neuroscience 2019Quote: ... Final purification was performed by size-exclusion chromatography and the purity of the proteins was assessed by 4%-15% SDS-PAGE gels (Bio-Rad) under reducing conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... The samples were then resolved using native PAGE (15% w/v polyacrylamide) and stained with ethidium bromide before visualization using the Gel Doc XR System (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts (15 µg) of protein lysates were heat denatured and separated on a 4–10% Mini-Protean Precast Gel (Bio-Rad), followed by transfer on a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The boiled samples were centrifuged at 14,000 rpm for 10 minutes and 20 μg of total protein per sample was then loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA) and ran at 140 V ...
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bioRxiv - Biophysics 2019Quote: ... The efficiency of labeling the oligonucleotides to α-actinin was estimated by a gel-shift assay (4%-15% gradient gel, Biorad) and determined as >95%.
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bioRxiv - Physiology 2020Quote: Electrophoresis was performed using a BioRad Mini-PROTEAN cell with 4-12% or 4-15% precast TGX gels (BioRad, Hercules, CA) in TGS running buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 to 25 μg for experiments that were imaged digitally) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run either at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Physiology 2020Quote: ... and cleared lysates were then denatured for 15 min at room temperature in SDS sample buffer and electrophoresed in parallel with fluorescent mass markers (Dual Color, Bio-Rad) on 10% SDS polyacrylamide gradient gels ...
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bioRxiv - Cancer Biology 2020Quote: ... The lysates were loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Cat# 4561086, Bio-Rad, Hercules, CA), and proteins were subsequently transferred onto a polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... Samples were checked for purity and presence of protein using 15% SDS-PAGE (Polyacrylamide gel electrophoresis, Mini Protean 3 System, Bio-Rad) and Coomassie Brilliant Blue (Merck ...
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bioRxiv - Microbiology 2020Quote: ... The protein samples were separated by either 15% SDS-glycine-PAGE or SDS-tricine-PAGE including the Precision Plus Protein Dual Xtra molecular weight marker (Bio-Rad). To run samples under nonreducing conditions ...
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bioRxiv - Biophysics 2020Quote: ... 15 μg of proteins were loaded onto gels prior to conducting SDS-PAGE and transferring onto polyvinylidene difluoride membranes (Bio-Rad). Membranes were then incubated with monoclonal antibodies against Cyclin B1 (PC133 ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1× TBE buffer at 1.32 V/cm for 14 hr at room temperature with buffer circulation in a Sub-cell GT electrophoresis system (15 × 20 cm gel, Bio-Rad). The gel was stained with 1× TBE buffer containing 0.3 μg/ml of ethidium bromide and photographed ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX, Biorad, Munich, Germany) and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2019Quote: ... and lysed then immunoprecipitated as described above using homemade rabbit anti-PGRN antibodies bound to Affi-Gel 15 (Bio-Rad Laboratories). Samples were then mixed and boiled 5 minutes with 10mM DTT followed by alkylation by treating samples with a final concentration of 28 mM iodoacetamide ...