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Citations for Bio-Rad :
1101 - 1150 of 1612 citations for IL 15RA&IL 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... either on 7-12% Tris-glycine gels or gradient NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4-15%) (BioRad). List of antibodies and their working dilution used in this work are reported in (Table S4) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then proteins were transferred from the gel to polyvinylidene fluoride (PVDF) membrane for 52 minutes at 15 V in Trans Blot Semi Dry Transfer Cell (BioRad). The membrane was cut horizontally along the 25 kDa marker band to allow for probing with two different antibodies ...
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bioRxiv - Physiology 2021Quote: ... Membranes were washed with 4 x 15 min TBS-T washes prior to incubation with secondary antibody (goat anti-rabbit-HRP diluted 1:10,000, Bio-Rad) for 1 h on a shaker at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... a total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Microbiology 2019Quote: 45 µL of each IP elution was combined with 15 µL of 4X Laemmli sample buffer supplemented with BME (BioRad), boiled for 10 min at 95 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... gels were submerged into 0.5 µg/ml ethidium bromide in water for 15 minutes and imaged with Gel Doc XR+ system (Bio-Rad). Banding pattern of Mlh1+/− samples were compared to the controls to identify the allele with and without Mlh1 promoter methylation.
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bioRxiv - Cell Biology 2019Quote: ... Immunoblotting was performed by standard methods using 4-15% Mini-PROTEAN TGX precast polyacrylamide gels and nitrocellulose membranes (Bio-Rad). Ponceau S staining of membranes was routinely used to assess equal sample loading and transfer efficiency ...
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bioRxiv - Biochemistry 2021Quote: ... The gel was stained 15 minutes in 1x SYBR gold in TBE with gentle shaking and visualized using a Gel Doc EZ Imager (Biorad). Fragments between 26-34 nucleotides were excised and purified by Small-RNA PAGE recovery kit (Zymo Research ...
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bioRxiv - Cell Biology 2021Quote: ... ECM and RPE cell lysate (15-20 μg protein) were run separately on precast Mini-Protean TGX gels (Bio-Rad) and transferred onto PVDF membrane (Immobilon) ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were then loaded (15-20 μg of protein per well) in a 4–20% Precast Protein Gel (Biorad) and resolved by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Physiology 2021Quote: ... and proteins were stripped from beads by incubation in gel loading buffer with beta-mercaptoethanol and loaded to 4-15 % gels (Criterion; BioRad) for SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 4-15% SDS-PAGE gel (Bis-Tris Criterion™ TGX™ pre-cast gels, Bio-Rad) and transferred to PVDF membranes (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoblotting was performed with 4-15% gradient Mini-PROTEAN TGX precast polyacrylamide gels and nitrocellulose membranes (Bio-Rad, Hercules, CA). Membranes were blocked with 5% milk in TBS with 0.1% Tween 20 (TBST ...
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bioRxiv - Cancer Biology 2021Quote: ... thawed and centrifuged at full speed in an Eppendorf microcentrifuge for 15 minutes and protein content was assessed by Bradford assay (Biorad). Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins in loading buffer were boiled for 5 min before loading on to 12-15% SDS-PAGE gels using the Mini-Protean III gel system (BioRad). A protein transfer apparatus (BioRad ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein cellular lysates (20μg) were analysed by 4-15% gradient SDS-PAGE and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... Antibodies were affinity purified using either the peptides linked to SulfoLink beads (Pierce) or the Ascaris proteins (without GST) linked to a mix of Affigel-10/15 (BioRad).
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The other two lanes were incubated with 1xTris/glycine/SDS buffer for 15 min and then incubated with 2 x Laemmli sample buffer (BioRad) for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged at 14,000g for 15 min at 4°C and supernatant protein concentrations were determined by Bradford assay (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 30 and 36 hpi respectively on a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (4561083, Bio-Rad). Blotting was performed using the Trans-Blot Turbo Mini 0.2 μm Nitrocellulose Transfer Pack (1704158 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x with 1X TBST for 15 minutes and developed using Clarity Western ECL Substrate (Bio-Rad, #1705060) and Molecular Imager Gel Doc (Bio Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515 ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein was determined directly on the membrane using the 4-15% Mini-PROTEAN TGX-Stain Free Protein Gel system (BioRad). Antibodies used for Western blotting analysis were ...
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bioRxiv - Neuroscience 2023Quote: Three biological exosome samples (20 μg/sample) were separated on 4-15% mini-protein TGX precast protein gels (Bio-Rad) and subsequently stained with Coomassie Blue ...
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bioRxiv - Synthetic Biology 2022Quote: ... the hybrid RNA/DNA origami was run on 1.5% agarose gel in TAE buffer (75 mL were poured into a 15 cm x 10 cm gel tray, Biorad) and analysed by in-gel imaging using the specific dye DFHBI-1T as described above with few modifications due to the different gel composition (agarose instead of polyacrylamide ...
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bioRxiv - Neuroscience 2022Quote: ... were resolved by SDS-PAGE using stain-free precast gels (4-15% Mini-Protean Tgx Stain-Free Protein Gels, Biorad). Gels were then activated by Ultraviolet light with the ChemiDocTM Touch Imaging System (Biorad ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
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bioRxiv - Physiology 2022Quote: Equal amounts of protein extracts were resolved by SDS–PAGE using 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to PVDF membrane ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 15 minutes in 1x TBST solution and incubated for 5 minutes at room temperature with either Clarity Western ECL (BioRad) or Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) ...
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bioRxiv - Pathology 2023Quote: ... Proteins were resolved on 4-15% SDS-PAGE gels and transferred to nitrocellulose membrane (Trans-Blot Turbo RTA transfer kit, #1704271, Biorad). Membranes were blocked for 1h at room temperature in 5%BSA-TBS-T (TBS-0.1%Tween 20 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were resolved on 4-15% SDS-PAGE gels and transferred to nitrocellulose membrane (Trans-Blot Turbo RTA transfer kit, #1704271, Biorad). Membranes were blocked for 1h at room temperature in 5% BSA-TBS-T (TBS-0.1%Tween 20 ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenized protein samples were combined with 4x sample buffer and 25μM DTT and incubated at 100°C for 10 minutes followed by loading into a 4-15% gradient pre-cast gel and transferred onto a nitrocellulose membrane through the Trans-Blot Turbo system (BioRad). The membrane was blocked in 5% milk in tris buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... 5uL of protein sample was loaded onto a 4-15% Mini-PROTEAN TGX Precast polyacrylamide gel from Bio-Rad (4561086). Protein samples were resolved and transferred to a PVDF membrane using the Trans-Blot STurbo Transfer System ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant fractions were normalized according to the OD600 of the bacterial culture at the time of harvest and samples were loaded on a 4-15% polyacrylamide gradient gel (BioRad), subjected to SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... Samples were run on a 15% polyacrylamide gel (LpxC western blots) or 4–20% Mini-PROTEAN gels (BioRad cat# 4568095) and transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2023Quote: The previously obtained plasma (1 µl) and gut mucus (30 µl) were separated on a 4-15% Mini-PROTEAN TGX gel (BioRad) under non-reducing conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... 10-20 µg/lane total protein was electrophoresed on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) with SDS running buffer and transferred to Polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2022Quote: ... Protein lysates were combined with Laemmli buffer and separated on a 4-15% Mini-PROTEAN TGC precast gel (Bio-Rad) by electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... The protein sample was denatured and run on 4-15% Mini-PROTEAN TGX Precast Protein Gels as per the manufacturer’s instructions (BIO-RAD). Blots were probed overnight at 4°C with antibodies recognizing GAD67 (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... 30 µg of protein were diluted in Laemmli buffer and loaded in a 4 % to 15 % gradient mini-protean TGX gel (BioRad). After electrophoresis run ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2023Quote: ... diluted in PBS for 15 mins and then permeabilized with 0.1% Triton X-100 (PBST, BioRad Laboratories, Hatfield, PA, USA) in PBS for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... were resolved on SDS 8-15% polyacrylamide gels and transferred onto nitrocellulose membranes using the Trans-Blot turbo transfer system (BioRad). Membranes were blotted with the primary antibodies against ADMA (Epicypher ...
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bioRxiv - Molecular Biology 2023Quote: ... Obtained samples were denatured in a 1xLaemmli buffer at 95°C for 10 min and proteins separated using electrophoresis on a 4-15% mini-protean-TGX-precast-gel (BioRad). Proteins were then transferred on nitrocellulose membranes by a semi-dry method using transblot turbo transfer system (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cell Biology 2022Quote: ... 10-50 μg protein was loaded on 10% acrylamide home-made or gradient (4-15% acrylamide) commercial (BIO-RAD, 4561086) Tris-Glycine-SDS gels along a protein ladder (PageRuler prestained protein ladder ...
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bioRxiv - Cancer Biology 2022Quote: ... 95°C for 2 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min (BioRad). The following primers from Integrated DNA Technologies (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... Purified GST-KIN-EC-ter fusion protein from pooled gel filtration fractions was cross-linked to Affi-gel 15 beads (BioRad) in MOPS ...