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Citations for Bio-Rad :
1501 - 1550 of 2025 citations for 7 CHLOROTHIENO 3 2 B PYRIDINE 6 CARBOXAMIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 2-ul of cDNA was added to qPCR reaction containing iQ SYBR green master mix (Bio-Rad) and 10-pmol of gene-specific primers ...
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bioRxiv - Microbiology 2022Quote: ... 400 Ω) was performed in a 2-mm cuvette using the Gene Pulser Xcell Electroporation System (BioRad). After electroporation ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 minutes at 72°C using the C1000 Touch Thermal Cycler (Bio-Rad, Los Angeles, CA). Primer sequences are listed in the Supplemental Methods.
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bioRxiv - Cell Biology 2022Quote: ... The detergent was then removed by four successive additions of Bio-Beads SM-2 Resin (Bio-Rad) over the course of ∼24 h at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was mixed with sample loading buffer supplemented with 1% v/v 2-mercaptoethanol (BioRad, Cat #1610710). Sample was not heated to prevent aggregation of the GPCR ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μL of the diluted samples were used as input in a ddPCR supermix (Bio-Rad, 1863023). Primers and probes ...
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bioRxiv - Biochemistry 2024Quote: ... A hydroxyapatite column was prepared by resuspending 2 g Bio-Gel HTP Hydroxyapatite (Bio-Rad, 130-0420) in 12 ml CDC6 HTP-wash buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4-15% Tris-Glycine gel (Bio-Rad Cat#5678084) using Tris-Glycine running buffer (0.2M Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 or 2 % agarose gel solutions (50 mL total volumes) were prepared with agarose powder (Bio-Rad) using 0.5× TBE buffer (45 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... or at 100 V for 2 h at 4 °C using the wet transfer method (Bio-Rad Mini Trans-Blot Electrophoretic Cell 170-3930) ...
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bioRxiv - Biochemistry 2022Quote: ... with integration time set to 2 s and imaged using a Gel Doc XR system (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Lysis was performed by directly adding 2x Laemmli sample buffer (supplemented by 2-mercaptoethanol, Bio-Rad Laboratories) at a ratio of 1 µL per 0.1 mg of cell pellets and pipetting up and down ...
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bioRxiv - Microbiology 2023Quote: ... The relative gene expression was analysed by the comparative 2-ΔΔCt method using CFX 96 software (BioRad). Data were normalized with the endogenous control ...
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bioRxiv - Biophysics 2022Quote: ... DDM was removed by addition of a 100-fold excess (w/w) of biobeads SM-2 (Biorad) compared to protein for 4 h at 4 °C under gentle agitation ...
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bioRxiv - Neuroscience 2023Quote: ... SARS-CoV-2 RT-qPCR reactions (10 µL) using the iTaq Universal Probe One-Step Kit (BioRad) and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed in sample buffer (2× Laemmli sample buffer, No. 1610737, Bio-Rad, Hercules, California, USA). Whole-cell lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-rat TCRαβ antibody (2 µg/rat dissolved in saline solution, clone R73, Bio-Rad #MCA453GA) from day 9 to day 14 to deplete DRG T cells as previously described 66 ...
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bioRxiv - Physiology 2023Quote: ... Captured complexes were dissociated from the beads in the ×2 Laemmli buffer (Bio-Rad Laboratories, Hercules, CA) and analyzed by SDS-PAGE/western blotting using chemiluminescent substrate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining was done in 2% milk-TBST (+ 0.01% sodium azide) and detected using ChemiDoc (Biorad).
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bioRxiv - Biochemistry 2024Quote: ... Detergent removal was carried out at 4°C via incubation with Bio-Beads SM-2 (Bio-Rad) at a concentration of 200 mg ml-1 for three times with two hours for each time ...
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bioRxiv - Plant Biology 2024Quote: ... centrifuged for 2 minutes before running through 4-20% mini Protean TGX Stain Free Gel (BioRad, USA). The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore ...
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bioRxiv - Systems Biology 2024Quote: ... containing 2 μl of cDNA template was prepared and analyzed using CFX Connect Real-Time instrument (BioRad) with the following thermocycling conditions ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with 5 μg/ml anti-Igκ plus anti-Igλ F(ab’)2 antibodies (BioRad). After 5 min of data acquisition ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended to OD 10 in 1X Laemmeli Buffer supplemented with 2-mercaptoethanol (10%) (Bio-Rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR (qPCR) was performed with primer sets corresponding to primer list (Table 3) and using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 172-5125) on an Applied Biosystems 7500 Fast Real-Time PCR system or ThermoFisher Scientific QuantStudio 3 or 5 Real-Time PCR system ...
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bioRxiv - Biochemistry 2022Quote: ... The plate was centrifuged at 2000rpm (3 minutes at room temperature) and placed in the CFX Touch™ Real Time PCR Detection System (Bio-Rad; Hercules, California, USA) set for an initial denaturation (95°C ...
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bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of clarified lysate (3 x 106 parasite equivalents) were resolved by SDS-PAGE (Mini-PROTEAN®TGX protein gel; Bio-Rad, Hercules, California, USA), transferred to PVDF membrane (Immobilon®-FL ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed again with 1x PBS-T (0.075 %) 3 times 10 minutes each and incubated with 1:1 ECL chemiluminescence solution (Clarity Western ECL, #170-5061, Bio-Rad Laboratories, Hercules, CA, USA). Signal was detected using an Amersham AI600 imager (Supplementary Table 5).
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bioRxiv - Cell Biology 2020Quote: ... A reaction solution of 20 Δl comprising 10 μl 2× Sso Advanced universal SYBR Green Supermix (Bio-Rad), 1 μl cDNA template (25 ng) ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... protein extracts were prepared in lysis buffer with 2% SDS and resolved in a 4-12% gel (Biorad). Membranes were probed overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR experiments were performed according to an absolute quantification design on the Opticon 2 PCR machine (Biorad). Β2Microglobulin (B2M ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 μl ddPCR reactions containing 10 μl of 2× QX200™ ddPCR™ EvaGreen® Supermix (Bio-Rad), 1 μl of 5-500 fold TET buffer-diluted stool DNA or 1 μl of TET buffer (for no-template controls ...
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bioRxiv - Cancer Biology 2021Quote: 2 ⨯ 106 CAR T cells collected after 24-day expansion were resuspended with 1 ⨯ Laemmli sample buffer (BIORAD) containing 2.5% β-mercaptoethanol before sonication with 50% amplitude in Sonic Dismembrator (FisherBrand) ...
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bioRxiv - Bioengineering 2021Quote: ... First strand cDNA was synthesized utilizing 2 µg of total RNA and iScriptTM cDNA Synthesis Kit (Bio-Rad). A fraction (0.75 μg ...
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bioRxiv - Bioengineering 2021Quote: ... 2 uL of bacterial culture was mixed with 10 uL 2X Sso Fast Evagreen qPCR mixture (BioRad USA). The primers were mixed into an equal forward/reverse (F/R ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA synthesis kit (Bio-Rad). A 1/10 dilution of cDNA was used for qRT-PCR analysis using the FastStart Universal SYBR Green Master Mix (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a nitrocellulose membrane for 2 hours in a wet blot tank system (Bio-Rad), and membranes were blocked with 5% skim milk for 30 min at room temperature before incubating with primary antibody ...
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bioRxiv - Microbiology 2021Quote: ... using primers specific for fathead minnow β-actin (Table 2) with iTaq universal SYBR green supermix (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... IPG strips were run upon SDS PAGE (12.5% acrylamide) using the Protean® II 2-D Cell (BioRad). Gels were then Coomassie blue stained (Phastgel Blue R ...
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bioRxiv - Bioengineering 2022Quote: ... ddPCR reactions were set up as follows: 11 μL 2×ddPCR™ supermix for probes (no dUTP) (BioRad), 0.2 μL FWD primer (100 μM) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products were confirmed by 2% agarose gel electrophoresis stained with GelRed using a 50 bp marker (BioRad). PCR products were normalized using Mag-bind Total Pure NGS ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×106 cells (extracted tissues or cell culture) were suspended in 50 μL RIPA (Radio ImmunoPrecipitaion Assay; BioRad) lysis buffer containing 200 mM PMSF (Alpha-Phenyl Methyl Sulfonyl Fluoride ...
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bioRxiv - Genomics 2022Quote: ... These products were analyzed on a 2% agarose gel and was imaged with Gel Doc imaging system (BioRad). The observation of multiple bands implied successful targeting of the sequences by the sgRNA.
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bioRxiv - Microbiology 2022Quote: ... Quantitative PCR was performed on 2 μl with the SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and the following primer pairs ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in agarose insert buffer and mixed 1:1 with 2% low-melting agarose (Bio-Rad). Gel inserts were solidified at 4°C for overnight and were stored in agarose insert buffer until analysis ...
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bioRxiv - Systems Biology 2019Quote: ... 272 mM sucrose) were mixed with 2 µg of DNA in a 1mm gapped electroporation cuvette (Bio-Rad). Cells were incubated on ice for 15 min and electroporated using the Gene Pulser XCell™ electroporation system (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Both PK-treated and untreated samples were then mixed 1:2 with 4X Laemmli sample buffer (Bio-Rad) containing DTT ...
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bioRxiv - Genetics 2019Quote: ... The resulting cDNA was PCR amplified and run on a 2% Certified Low Range Ultra Agarose (Bio-Rad) gel with subsequent extraction using the QIAquick® Gel Extraction Kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... and then the WBC solutions were embedded into 2% agarose plugs (CHEF Genomic DNA Plug Kit, Bio-Rad) to avoid fragmentation of long DNA molecules ...