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Citations for Bio-Rad :
1451 - 1500 of 2025 citations for 7 CHLOROTHIENO 3 2 B PYRIDINE 6 CARBOXAMIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... stained for >2 hours with Bio-Safe™ Coomassie Stain (#1610786, Bio-Rad Laboratories, Inc, USA), and de-stained in water for > 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... smegmatis MC2155 at 2.5 kV in a 2 mm cuvette using a MicroPulser device (Bio-Rad). Liquid starter cultures were grown in 7H9 Middlebrook medium (7H9 Middlebrook base [HiMedia] ...
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bioRxiv - Systems Biology 2024Quote: ... and run on precasted gels (Criterion 12+2 well comb, 45 µl, Bio-Rad Laboratories Inc). The gel was run at (Criterion ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... were heated at 99 °C for 3 minutes and subjected to 4-20% gradient SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad Laboratories, Inc.). Membranes were then blocked for 1 hour with 5% (w/v ...
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bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
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bioRxiv - Cell Biology 2021Quote: ... Band density was normalized according to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) content and analyzed using Image Lab software (Bio-Rad, California, USA) (29).
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 3% H2O2 for 15 min at room temperature and then incubated with goat anti-rabbit IgG-HRP (Bio-Rad 170-6515) secondary antibody diluted 1:300 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Genomics 2024Quote: ... 4.5 µl of sample were used to generate single cell RNA-Seq libraries by following the Illumina Bio-Rad SureCell WTA 3’ Library Prep Guide (Illumina, San Diego CA and Bio-Rad, Hercules, CA) and using the ddSEQ Isolator for Single Cell Sequencing (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for DpnII digested DNA or primers 3 and 4 (table S2) for NlaIII digested DNA on a thermal cycler (Bio-Rad C1000 Touch). PCR amplified samples were purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were denatured at 95 °C for 3 minutes prior to loading onto a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Biorad Cat. No. 4563066). Gels were transferred to a nitrocellulose membrane (Millipore Sigma Cat ...
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bioRxiv - Microbiology 2023Quote: ... were detected as dark spots after a 10-min reaction with 5-bromo-4-chloro-3-idolyl phosphate and nitro blue tetrazolium using an alkaline-phosphatase-conjugate substrate (Bio-Rad, CA, USA). SFUs were counted using the AID ELISpot Reader System (Autoimmun Diagnostika) ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell suspension then was divided equally and heated for 3 min at indicated temperatures on T100™ Thermal Cycler (Bio-Rad Laboratories). Cells were snap-frozen and thawed twice and centrifuged at 17.000 g for 15 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... for the selected clinical isolates using the indicated concentrations of antibiotics via an alamar blue reduction assay completed as described but without shaking.8 After 3 days of incubation with the alamar blue reagent (BioRad, Hercules, CA, USA), the MIC was identified as the lowest concentration of drug that inhibited the reagent color change as determined visually.
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bioRxiv - Biophysics 2023Quote: ... Migration of the DNA in the gel was visualized via the fluorescent Cy-3 label (ChemiDoc™ MP Imaging System, BIO-RAD, USA). Protein-DNA complexes were transferred from the gel to the PVDF membrane (Immun-Blot® PVDF Membrane ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for DpnII digested DNA or primers 3 and 4 (table S3) for NlaIII digested DNA on a thermal cycler (Bio-Rad C1000 Touch) with the block settings shown in table 1 ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL primer 556R (5’ CTTTACGCCCARTRAWTCCG 3’) at 10 µM and 10 µL SYBER® Green Master Mix (Bio-Rad, Veenendaal, The Netherlands). The DNA extraction estimated an average rRNA yield corresponding to 5×10^8 bacterial cells/mL.
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bioRxiv - Molecular Biology 2024Quote: ... 3-10 μg total protein was loaded and run on AnykD™ Criterion™ TGX™ Precast acrylamide protein gels (Biorad cat#5671125) then transferred to nitrocellulose (Biorad cat#1704159) ...
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bioRxiv - Microbiology 2024Quote: ... and R-tot (5′-CTGCTGCCTCCCGTAGGAGT-3′) in the Rotor Gene 6000 thermocycler (Corbett Research, Sydney, Australia) with the iTaq Universal SYBR Green Supermix (Bio-Rad, Reinach, Switzerland).
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bioRxiv - Bioengineering 2021Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 4°C during the LC-MS measurement ...
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bioRxiv - Microbiology 2020Quote: ... The plate was washed 3 times prior to adding 50 μl of 1:1000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Bio-Rad, Cat# 172-1011) to the plate and incubated for 1 h at RT ...
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: Frozen muscle biopsies were homogenised in modified Laemmli sample buffer as described previously.37 Samples were run in Bio-Rad Criterion 3–8 % tris-acetate gradient gels (Bio-Rad Laboratories, CA, USA) at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the membranes were washed 3 times for 10 min each with TBST buffer at 24 °C and visualized using enhanced chemiluminescence reagents (Bio-Rad Laboratories, CA, USA). The images were captured using Image Quant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... and O’nyong yong virus (ONNV) were subjected to a thermal gradient treatment from 30 to 60 °C for 3 h with a thermocycler (Bio-Rad T100 Thermal Cycler), after which samples were immediately titrated on Vero cells ...
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bioRxiv - Microbiology 2022Quote: ... the indicated ESA supernatant was boiled in SDS-PAGE sample buffer and 3 μg of each sample was run with a 4-15% TGX gradient gel (Bio-Rad cat. no. 4561086), then transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, 1725121). Each reaction proceeded at 50 °C for 2 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and the miR200c primers (upstream primer, 5’- TAATACTGCCGGGTAATGATGGA-3’) (Eurofins Genomics) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). U6 primers (TAKARA ...
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bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 seconds at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 10°C during the LC-MS measurements ...
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bioRxiv - Microbiology 2023Quote: ... One microliter of each sample was mounted on a layer of 1.2% (w/v) agarose in 1:3 (vol/vol) TSB/PBS placed on a glass plate (Bio-Rad Mini-PROTEAN Short Plate) with a coverslip placed on top of each sample ...
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bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180 °C using a plate sealer (PX-1; Bio-Rad, Hercules, CA, USA) and maintained at 10 °C during the LC-MS measurements ...
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bioRxiv - Immunology 2019Quote: ... All patients were HIV tested using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Microbiology 2019Quote: ... Relative fluorescence was measured at 5-minute intervals for 2 hours in a CFX96 system (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... containing 2 ml of anion exchange resin AGMP-1M (100-200 mesh, chloride form) (BIO-RAD, 1411841). The resin was first cleansed three times with 10 ml 18.2Ω water followed by 7 ml 0.5N HNO3 ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 μl of gene-specific primer pair and 5 μl iTaq Universal SYBR Green Supermix (Bio-Rad). The relative transcript abundance of each gene (normalized to rpl4 ...
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bioRxiv - Microbiology 2021Quote: ... fixed with methanol supplemented with 2% H2O2 and incubated with a biotinylated anti-RSV antibody (Bio-Rad) in PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... ramp rate set at 2 °C/sec] was performed in a thermal cycler (Bio-Rad C1000 Touch). Droplets were quantified in a QX200 Droplet Reader (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: Cells were mounted on a 2% agarose pad containing resuspension medium using a gene frame (Bio-Rad). Cells were concentrated by centrifugation (3300g for 30 s ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at room temperature before signal development with Western ECL Substrate (Bio-Rad Laboratories, #1705061) and imaging under ChemiDoc XRS+ System (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2022Quote: ... 2 μl of diluted vRNP mixtures was loaded onto a 5% polyacrylamide native TBE gel (Bio-Rad) and run at 125 V for 80 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... boiled for 2 min and then analyzed on a 4–20 % SDS-PAGE gradient gels (Bio-Rad). The protein bands were then transferred to a nitrocellulose membrane ...
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bioRxiv - Immunology 2019Quote: ... fluorescence images were acquired before and after a 2 min incubation after trypan blue (0.1%; Bio-Rad), which was added carefully to minimize sample movement ...
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bioRxiv - Cell Biology 2019Quote: ... RT-qPCR was performed on 2 μl of 1/25 diluted cDNA with a CFX-96 (Biorad) thermocycler ...
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bioRxiv - Immunology 2019Quote: ... IL-2 mRNA amounts were determined with a SYBR Green PCR master mix from Life Technologies (4344463) relative to mRNA for β-2 microglobulin on a DNA Engine Opticon II System (Bio-Rad) using the following oligonucleotides ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.009% H2O2) for 2 min and images were taken with a ChemiDoc Imaging System fitted (Bio-Rad). Densitometry was performed with Image Lab 5.0 software (Bio-Rad) ...
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bioRxiv - Genomics 2021Quote: ... RT-PCR amplicons were electrophoresed on 2 % agarose gels and imaged on a ChemiDoc MP (Bio-Rad). The resulting images were analyzed using ImageJ (v1.52a) ...