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Citations for Bio-Rad :
1601 - 1650 of 2025 citations for 7 CHLOROTHIENO 3 2 B PYRIDINE 6 CARBOXAMIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Each qRT-PCR reaction of CZ and ZC was performed by the manufacturer’s instructions of EvaGreen Express 2×qPCR MasterMix (abm, Cat. No.MasterMix-ES, http://www.abmGood.com/) and BioRad®CFX96 Real-Time PCR system (Bio-Rad). Relative expression was quantified with the geometric mean of internal reference genes ETIFI (eukaryotic translation initiationfactor 1) ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 detection was performed on the CFX96™ Real-Time PCR Detection System (Bio-Rad, California, USA), using the NeoPlex™ COVID-19 Detection Kit (Genematrix ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad). The threshold for detection of fluorescence was manually adjusted to 40 Relative Fluorescent Units to obtain the same threshold for each plate ...
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bioRxiv - Cell Biology 2020Quote: ... Total numbers of viable cells were determined after 2 and 4 days using the TC10 cell counter (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR reactions were conducted using 10 µL 2× SYBR-Green Universal Master Mix (Bio-Rad, Hercules, CA, USA), 2 µL of forward primer (9 mM) ...
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bioRxiv - Immunology 2021Quote: ... Bound PRRSV-2-specific IgG was detected using goat anti-pig IgG conjugated with horseradish peroxidase (HRP; Bio-Rad) diluted at 1:10,000 in blocking buffer for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: Frozen PBMCs were thawed, washed in FACS buffer (2% FBS, 1mM EDTA in PBS) and counted on TC20 (Biorad) before surface staining using the following panel ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL of cDNA was mixed with 2 μL of forward and reverse primers (2.5 μM each, Table S1,) and 1x SYBR Green Supermix (BioRad) for a final reaction volume of 20 μL ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein levels were quantified using BCA Assay (Pierce) and supplemented with 4x Laemmli Buffer with 2-mercaptoethanol (Biorad). Samples were boiled and sonicated ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells:DNA mixture was transferred to an ice-cold 2 mm gap electroporation cuvette (Molecular Bioproducts) and electroporated at 2.5 kV with a MicroPulser (BIORAD). One ml of Super Optimal broth with Catabolite repression (SOC ...
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bioRxiv - Plant Biology 2022Quote: ... Each RT-qPCR reaction (10 μl) included 2 × iTaq™ Universal SYBR®Green Supermix (Bio-Rad, CA, USA), 500 nM forward and reverse primers (Supplementary File 1C) ...
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bioRxiv - Molecular Biology 2022Quote: ... The SARS-COV-2 NTD expression was analysed with SDS-PAGE followed by Western-blot on PVDF membrane (Biorad) using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences) ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitants were then gently washed three times with KPBS and eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis.
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bioRxiv - Molecular Biology 2024Quote: ... 2 µL of cDNA was added to 10 µL of Bio-Rad ddPCR Supermix for Probes (1863024; Bio-Rad), followed by the addition of 1 µL of TaqMan miR-146a-5p 20X primer mix (4427975 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT reactions were diluted 2-fold and qPCR was performed in duplicate with SsoAdvanced SYBR Green Supermix (Bio-Rad) according to manufacturer’s protocols with qPCR_FWD and qPCR_REV primers from IDT (see below) ...
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bioRxiv - Developmental Biology 2024Quote: ... centrifuged at 14,000rpm at RT for 2 minutes and separated on precast 10% SDS-PAGE gels (4561036, Bio-Rad) at 100V for 70 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 μL cDNA template were performed in technical duplicate wells on a C1000 Touch Thermal Cycler (Bio-Rad) with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... 2 % fetal bovine serum and 8 % chelated fetal bovine serum using Chelex® 100 Resin (BioRad, Ref. 142-1253). To differentiate HaCaT cells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1×108 concentrated fungal protoplasts (37 μL) (64) were mixed with 2% CleanCut Agarose (63 μL, Bio-Rad, #1703594). The mixture was then loaded into a plug mold (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... the concentration was calculated by the Bradford assay using a Quick Start Bradford Protein Assay Kit 2 (Bio-Rad).
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bioRxiv - Immunology 2023Quote: Diluted mouse serum (1:100 in 0.1% BSA in PBS) was incubated on HEp-2 slides (MBL or BioRad) for 1 hour in the dark at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... The heart tissue samples were then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 20% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Microbiology 2023Quote: ... using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Plasmid sequences were confirmed by sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... Each 100 μl aliquot was transformed with 0.1 pmol of EZ-Tn5
Tnp Transposome using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Each reaction was allowed to recover in 500 μl SOC (2% tryptone ... -
bioRxiv - Microbiology 2023Quote: ... 2 min at 72°C for final extension in a T100 Thermal Cycler (BIO-RAD Lab. Inc., Hercules, CA). The sizes of PCR products were checked by running agarose gel electrophoresis ...
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bioRxiv - Biophysics 2023Quote: ... slides were prepared with unlabeled microtubules attached to the slide surface via an anti-tubulin antibody (BioRad YL1/2). Kinesin-liposome complexes were added to the flow cell and imaged in TIRF ...
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bioRxiv - Biophysics 2023Quote: ... Bio-Beads SM-2 (#152-3920) and the enhanced chemiluminescence (ECL) western blotting reagent (#170-50001) from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... MEC samples were directly homogenized using 2x Laemmli Sample Buffer and supplemented with 2-mercaptoethanol (β-mercaptoethanol) (BioRad Hercules). Proteins were separated by electrophoresis on a polyacrylamide gradient gel (BioRad Hercules ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated at 60°C for 2 h on a Xenium thermocycler adaptor plate (BioRad, C1000 Touch Thermocycler), followed by deparaffinized through a series of immersions in xylene ...
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bioRxiv - Biochemistry 2024Quote: A 22ul reaction mixture was prepared comprising 11ul 2×ddPCR Supermix for probes (no dUTP) (Bio-Rad, CA, USA), 0.55 ul of TRECs primers (36uM stock ...
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bioRxiv - Microbiology 2022Quote: ... and 1’,3’-bis[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho]-glycerol (16:0-18:1 Cardiolipin) were spotted using a Hamilton syringe onto a nitrocellulose membrane (Biorad trans-blot turbo RTA Midi 0.2 µm) to yield 10 ...
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bioRxiv - Pathology 2019Quote: ... Capillary collection was performed by fingerstick with a lancet (Unistick 3, Owen Mumford, 30G, 1.5mm) using the Biorad variant II capillary collection device (Bio-Rad catalog number 196-2050, BioRad 510(k) K142448) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were pelleted and washed once with 0.75 ml of ice cold 0.5% TCA and resuspended in 2× Laemmli sample buffer (Bio-Rad Laboratories). Western blot was performed as described (Hsu et al. ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...
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bioRxiv - Neuroscience 2019Quote: ... samples were prepared on ice (to a final volume of 40 microliters) in a mixture of Laemmli Sample Buffer and 2-Mercaptoethanol (BioRad) and then vortexed and denatured for 10 min at 70°C ...
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bioRxiv - Microbiology 2019Quote: ... boiled (95°C, 10 min), centrifuged (1,000 × g, 2 min) and loaded on 10% or 4-20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Plant Biology 2019Quote: ... 2% of samples were used as templates to perform quantitative PCR (qPCR) using SYBR Green PCR Master Mix (Bio-Rad) with primers recognizing the Ds element or an unrelated intergenic region in soybean chromosome 7 ...
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bioRxiv - Developmental Biology 2019Quote: ... monomeric ATP-bound 2 μM rabbit muscle actin was treated overnight at 4°C with analytical grade anion exchange resin (BioRad), hexokinase (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: ... Peak fractions from SEC purification were pooled and 2-mercaptoethanol was removed with Econo-Pac 10DG desalting column (Bio-rad) which was pre-equilibrated with buffer C ...
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bioRxiv - Cancer Biology 2020Quote: ... ran at 120 V for ∼2 h and transferred at 100 V for 1 h onto nitrocellulose membranes (Bio-rad) using the wet blot method ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was diluted to 10 ng/µland 2 µl was used in each RT-qPCR reaction with 10 µl iTAQ Universal SYBR green supermix (BioRad) and 400 nM primers ...
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bioRxiv - Cell Biology 2020Quote: ... Detergent was removed by three addition of BioBeads SM-2 adsorbent resin (25 mg per 1 mL of lipid mixture) (BioRad) for 2 h each at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto 0.2 µm pore nitrocellulose membranes for 10 min at 2.5 V using the Trans-blot turbo transfer system (Bio-Rad). For more sensitive detection of neurexin levels ...
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bioRxiv - Cell Biology 2021Quote: ... 15% for FKB-2) and transferred onto nitrocellulose membranes for 1 hour at 200 mA (SemiDry transfer blot, Bio-Rad). After incubation with blocking solution (LICOR Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... 95°C), and run (loaded in 2 wells, 20 μL per well) on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mg/ml Orange G), denatured (2 min, 80°C) and run on a 10 well 10% TBE-urea gel (Biorad) (200 V ...