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Citations for Bio-Rad :
1301 - 1350 of 2025 citations for 7 CHLOROTHIENO 3 2 B PYRIDINE 6 CARBOXAMIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... LAMP reaction products were analysed on 2% agarose gels (65V for 3 hr) stained with ethidium bromide (10 mg/mL) and viewed on Gel Doc XR Imaging System (BioRad, USA).
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were washed in 1X PBS with 0.1% Tween 3 times for 5 minutes each before scanning using the ChemiDoc™ MP Imaging System (Bio-Rad). Note ...
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bioRxiv - Molecular Biology 2022Quote: ... The 10 µL cDNA reaction was diluted to 200 µL and 3 µL was used with iTaq Universal SYBR Green Supermix (Bio-Rad) and 670 nM of primer pairs (Table S4 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The relative amounts of the resulting products were analyzed by scanning the gels and determining the intensities of ethidium bromide stained bands using Image Lab software Version 1.36b (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA from individual cells were barcoded and libraries prepped according to the 10X chromium version 3 chemistry using the C1000 Touch thermal cycler (Bio-Rad). Quality of prepped cDNA and resultant libraries were assessed with the Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2024Quote: ... Detection was performed by incubating the membranes with ECL Select Western Blotting Detection Reagent (GERPN2235 – Cytiva) for 3 minutes before detection using a ChemidocMP (Bio-Rad) or Alliance LD2 Imaging System (UviTec Cambridge).
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bioRxiv - Molecular Biology 2024Quote: ... then incubated with ProSignal Pico Spray chemiluminescence substrate (Prometheus) for 1-3 minutes at room temperature and imaged using a ChemiDoc Imaging System (Bio-Rad). Contrast was occasionally adjusted to improve visualization of bands ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were washed 3 times for 10 minutes each and then developed with the Clarity or Clarity Max ECL Western Blotting Substrates (Bio-Rad). The chemiluminescence detection was performed using the Chemidoc MP system (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then exposed to the ECL substrate kit (Pierce 34850 and 34095) for 3 minutes before imaging with the ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... Dialysis was repeated against another 1 L of low-salt buffer for 3 h before application to a MonoQ (MonoQ HR 10/10, Pharmacia) FPLC column (Biologic Duoflow system, Bio-Rad). The sample was eluted over 80 mL in 20 mM piperazine by applying a gradient from 0% to 30% of a high-salt buffer (20 mM piperazine ...
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bioRxiv - Microbiology 2023Quote: ... The gel was run at 125 V for 3 to 4 hours and the RNA was then transferred to a Zeta-Probe GT membrane (Bio-Rad) by capillary action with 20 x SSC buffer (3 M NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed a further 3 times in TBS-T and bound antibodies were visualised using the Clarity Western ECL substrate (BioRad; #1705061) with a ChemiDoc MP system (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... The slurry was gently stirred for 3 h to allow binding and then loaded into a glass Econo-Column (Bio-Rad). The column was washed with 10 Column Volumes (CV ...
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bioRxiv - Biochemistry 2023Quote: ... pre-run for 1 hour at 4 ° C and run for 1 hour at 120 volts on Mini-Protean 3 gels (Bio-Rad).
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times for 5 min with TBS and were subsequently incubated with Clarity Western ECL substrate working solution (Bio-Rad) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... and resolved for 4 hours at 120V for LapA and 3 hours at 115V for MapA in Mini-PROTEAN Tetra Vertical Electrophoresis Cells (Bio-Rad) with 1x Tris/Glycine/SDS buffer (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... This step was repeated for 3 times and the samples were concentration-matched at OD500nm = 10 and mixed 1:1 with 2x Laemmli buffer (Bio-Rad), containing SDS and 2-mercaptoethanol ...
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bioRxiv - Biophysics 2023Quote: ... Gels were run on ice at 140V for 3 hours using Bio-Rad Mini-Protean TGX precast gels in 1X Tris/Gly/SDS running buffer (BioRad #1610732). Gels were then equilibrated with transfer buffer (BioRad #1610734 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed 3 times with TBST for 5 min each and then the membrane was incubated with Clarity Western ECL Substrate (Bio-Rad) for 2 min before exposure to film and development in a darkroom.
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bioRxiv - Cell Biology 2024Quote: ... The resulting cDNA was diluted 1:20 in RNase-free H2O and 3 µl dilution were used for RT-qPCR analysis using iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions and using the following cycling protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3 times for 10 min with TBS-T and developed with an enhanced chemiluminescence (ECL Bio-Rad 1705061) substrate ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out with 3-5 μL of the linear PCR product (metH homology extension-flanked kan cassette) at 2.5 kV (Bio-Rad MicroPulser), and cells were immediately recovered with 1 mL SOC and cultured at 37°C for one hour ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was then eluted in 3 mL buffer A containing 300 mM imidazole before applying the eluate onto Econo-Pac 10DG column (Bio-Rad) equilibrated with HBS to remove imidazole ...
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bioRxiv - Immunology 2024Quote: ... After final wash and supernatant removal, 1x Loading Buffer (40 μL, 3:1 Lysis Buffer:4x Laemmli Loading Buffer (Bio-Rad)) was added to the sample and the sample boiled (10 min ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 μg of eluted RNA was used for cDNA synthesis employing iScript (Bio-Rad). Samples were analyzed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: Cell lysates were harvested by directly lysing the cells with 2 × sampling buffer (Bio-Rad). Proteins were separated by SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 x 106 cKit enriched cells were resuspended in 150 µl Laemmli buffer (BioRad 1610737) supplemented with 1:10 β-mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The amplified samples were run and cut from 2% low-melt agarose gels (Bio-Rad) and followed by sRNA sequencing with NextSeq500 (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rat Ly-6B.2 alloantigen (1:100, MCA771GA, Bio-Rad Laboratories, Hercules, CA) for neutrophils ...
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bioRxiv - Molecular Biology 2021Quote: Purified SARS-CoV-2 N protein was electrophoresed on 4-10% SDS-PAGE (Bio-Rad) and stained with Coomassie Brilliant Blue G-250 (CBBG-250) ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted fraction was precipitated with Ready Prep 2-D cleanup kit (Bio-Rad, USA). The precipitate was dissolved in O’Farrell lysis buffer and analyzed with 2D polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2019Quote: ... with a 5μl reaction mixture containing 2.5μl 2× SYBR Green MasterMix (Bio-Rad, Hercules, USA), 0.25μL of both 10μM forward and reverse primers and 2μl cDNA (5ng/μl) ...
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Interaction of a sarcolipin pentamer and monomer with the sarcoplasmic reticulum calcium pump, SERCAbioRxiv - Biophysics 2019Quote: ... Detergent was slowly removed by the addition of SM-2 biobeads (Bio-Rad, Hercules, CA) over a 4-hour time course (final ratio of 25 biobeads ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were transferred onto 0.2 µm (Aβ) or 0.45 µm (NUO-2) nitrocellulose membranes (BioRad) for 40 minutes at 70V ...
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bioRxiv - Biophysics 2021Quote: ... we added 60 mg Bio-beads SM-2 (Bio-Rad, Hercules, CA, USA, No. 1523920) and dialyzed the samples in detergent overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted proteins were mixed with a premade 2× Sample Buffer (Biorad, Hercules, CA, USA), omitting β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... The cell monolayers were then blocked with blocking buffer (2% Blotting-Grade Blocker [Bio-Rad] supplemented with 2% goat serum [Gibco] in PBS-Tween ...
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bioRxiv - Biochemistry 2022Quote: ... Mass calibration was done by using 2 mg/ml BSA standard (Bio-Rad, cat.5000206). PBS pH 7.4 was used as isocratic buffer ...
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bioRxiv - Physiology 2021Quote: ... The ddPCR reaction mixture consisted of 10 microliters of a 2× EvaGreen Supermix (Bio-Rad), 2 microliters of primers ...
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bioRxiv - Microbiology 2021Quote: ... Detergent was removed by incubation with 0.6 g/ml Bio-Beads SM-2 (Bio-Rad) at 4 °C for 2 hours ...
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bioRxiv - Bioengineering 2021Quote: ... samples were mixed 1:1 with 2x Laemmli sample buffer with 2-mercaptoethanol (Bio-Rad) and boiled for 10min ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM CaCl2) and blotted directly onto 0.45 μm pore size nitrocellulose membranes (Bio-Rad) using a BioDot SP slot-blot apparatus (BioRad ...
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bioRxiv - Molecular Biology 2019Quote: ... set at 2 copies (CNV2) on the Bio-Rad QX200 ddPCR system (Bio-Rad, CA) as per Codner and colleagues (Codner et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Unilamellar proteoliposomes were spontaneously generated by removing the detergent using SM-2 Biobeads (Bio-Rad). The average radii of the prepared proteoliposomes for the proton transport assays and CD measurements were 76.87±2.29 nm and 31.46±1.68 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred onto .2 um nitrocellulose membranes using the semi-dry turbo transfer system (Biorad). One hour incubation in 5% NFDM in tris-buffered saline .1% tween20 (TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of cDNA were analyzed using a CFX Connect real-time PCR machine (BioRad) with SensiFAST SYBR Lo-ROX Kit (Bioline) ...