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Citations for Bio-Rad :
1251 - 1300 of 2194 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Immunology 2021Quote: ... 1/10 dilutions were used in triplicates with 0.2 μM primer and 10 μL LightCycler 480 SYBR Green I Master in a 20 μL reaction and qPCR executed in a 98- well block on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Absolute transcript levels for TDP-43 ...
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bioRxiv - Cancer Biology 2019Quote: ... The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories, Inc.) and 0.25 µmol/L each primer ...
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was subjected to affinity chromatography at 4°C composed of 75 μL of amylose resin bound to 200 pmol of MS2-MBP protein in a Bio- Spin disposable chromatography columns (Bio-Rad). After washing ...
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bioRxiv - Bioengineering 2022Quote: ... Approximately 20 μL of the denatured phage was loaded in a 4-20 % gradient gel (Mini-PROTEAN® TGX Stain-Free gel, Biorad) and separated by electrophoresis at 120 V ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... isolates with a meropenem minimum inhibitory concentration (MIC) greater than 0.125 mg/L had rapid phenotypic confirmation of carbapenemase production using the β-carba test (Bio-Rad). In addition ...
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bioRxiv - Immunology 2020Quote: ... A chemiluminescent signal visualized by Chemi-Lumi One L (07880-70, Nacalai Tesque) was detected with a ChemiDoc Touch imaging system (Bio-Rad) and analyzed with Image Lab software version 6.0.1 (Bio-Rad).
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bioRxiv - Cancer Biology 2019Quote: ... This 20-μL reaction mix was added to a DG8 cartridge together with 70 μL of Droplet Generation Oil for Probes (Bio-Rad) and used for droplet generation ...
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bioRxiv - Biophysics 2020Quote: ... the dynamics of PEN reactions were recorded in 20 μL solutions inside 150 μL qPCR tubes using a Qiagen Rotor-Gene qPCR machine or a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). For biological relevance ...
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bioRxiv - Microbiology 2020Quote: ... Icecold freshly prepared competent cells (25 μl) were mixed with 4.5 μg of linear DNA and transferred to 0.1 cm-gap electroporation cuvettes (Bio-Rad, CA, USA). A pulse of 1.5 kV was applied to the cuvette with a MicroPulser electroporation apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... equipped with a diode array detector (L-7450) and separated on an AMINEX HPX-87H column (7.8 x 300 mm; BioRad, Munich, Germany) with a cation H guard pre-column ...
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bioRxiv - Biophysics 2022Quote: ... 5 µL of the soluble fractions of cell lysates were run on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad) and stained using Gelcode Blue (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: LTA extracts (10 μl) were run on 15 % polyacrylamide gels and transferred to PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad). After blocking with 5 % milk and 1 % BSA in TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... The 10 µL cDNA reaction was diluted to 200 µL and 3 µL was used with iTaq Universal SYBR Green Supermix (Bio-Rad) and 670 nM of primer pairs (Table S4 ...
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bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was set up in a 20-μl volume with 100 - 200 ng of RNA and was conducted using the CFX Connect Real-Time PCR System (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: ... 1x DreamTaq buffer and 1 μL sample DNA in a total reaction volume of 10 μL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then passed over 20 ml of Poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were then stained with Coomassie Brilliant Blue G (80 mg dissolved in 1 L water with 30 mM HCl) and destained with water before visualising using a ChemiDoc MP Imaging System (Bio-Rad). For mNeonTP-loaded encapsulins ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then passed over 20 ml of Poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was then passed over 20 ml of poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2024Quote: ... 1x Dream Taq buffer A and 1 μL sample DNA in a total reaction volume of 10 uL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Immunology 2024Quote: ... 20 µl of each reaction mix and 70 µl of oil were converted to droplets with the QX200 droplet generator (Bio-Rad). Droplet-partitioned samples were then transferred to a 96-well plate ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad, Hercules, CA, USA). After washing 3 column volumes with wash buffer (50 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... and O’nyong yong virus (ONNV) were subjected to a thermal gradient treatment from 30 to 60 °C for 3 h with a thermocycler (Bio-Rad T100 Thermal Cycler), after which samples were immediately titrated on Vero cells ...
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bioRxiv - Genetics 2020Quote: ... fitted with a Bio-Rad Aminex HPX-87H ion exclusion column (Hercules, CA) fitted with a micro-guard cation-H refill cartridge (Bio-Rad cat #125-0129) and UV/VIS diode array detector monitoring at 210 nm ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentration was assessed in cell culture supernatants 48 h post transfection by using immunoassays formatted on magnetic beads (Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y) (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol via Luminex 100 technology (Luminex ...
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bioRxiv - Developmental Biology 2023Quote: ... The membranes were washed with TBST three times and then incubated with a secondary antibody for 1 h before using Luminol/enhancer solution and ClarityTM Western ECL Substrate (Bio-Rad Laboratories, Inc. US). Western blot images were scanned by using Gel Doc XR system (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A solution of rabbit αFITC antibody (1 μg/mL, Bio-Rad 4510-7804) in 2% Milk-PBS was added to each well (100 μL/well ...
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bioRxiv - Neuroscience 2022Quote: ... Secondaries for immunoblots were HRP-conjugated rabbit antibodies (Bio-Rad Laboratories, 1:10000).
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were a-mouse or α-rabbit-HRP (1:5,000; Bio-Rad). Blots were developed using Clarity Western ECL Blotting Substrate (BioRad ...
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bioRxiv - Bioengineering 2020Quote: ... anti-infliximab (HCA213G) and anti-adalimumab (HCA205) were ordered from BioRad. Similarly ...
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bioRxiv - Immunology 2024Quote: ... Anti-F4/80 (rat anti-mouse MCA497A647, Biorad, dilution 1:500) and anti-Parp14 (sc377150 ...
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bioRxiv - Neuroscience 2021Quote: ... The blotted membranes were rinsed three times with 0.05% Tween 20 in Tris-HCl-buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against mouse IgG (Bio-Rad Laboratories) or guinea pig IgG (Bioss Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no. 1705061, Bio-Rad, Gladesville ...
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bioRxiv - Molecular Biology 2019Quote: ... The IgG fraction was purified from hybridoma culture medium by Protein A chromatography on a Profinia™ protein purification system (Bio-Rad) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were visualized with goat anti-mouse or goat anti-rabbit IgG secondary antibodies (1:5000) diluted in 2% Omniblot milk (AmericanBio) in 1X TBST using a chemiluminescence detection system (BioRad ChemiDoc MP).
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Immunology 2023Quote: Serum SARS-CoV-2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS-CoV-2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... proteins within the gel were partially transferred to polyvinylidene difluoride (PVDF) membrane (250 mA current flow, 1.5 h) using wet transfer apparatus (Bio-Rad Laboratories Inc., Hercules, CA, USA). Blocking of the PVDF membranes were done for overnight at 4°C temperature in shaking condition using blocking buffer (0.019 M Tris ...
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bioRxiv - Microbiology 2020Quote: The pure cultures were grown in TSAYE plates for 24 h and DNA was extracted using 2% Chelex solution (Chelex 100 resin, Bio-Rad laboratory, Hercules, California, USA). The colonies (2-3 mm ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA extracted from mouse tail tips (H. Liu et al., 2019) was subjected to PCR performed in a thermal cycler (T100, Bio-Rad Laboratories, Hercules, CA, USA). The WT and Ssh2 KO alleles were assayed by primers ...
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bioRxiv - Microbiology 2020Quote: ... smegmatis cells were transformed with 2 µL of the mycobacterial expression vector pNIT cloned with the gene Rv1630 (RpsA) using electroporation with a gene pluser (Bio-Rad, USA). Electrocompetent M ...
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bioRxiv - Molecular Biology 2019Quote: ... in a cartridge containing 20 µL of the reaction mix and 70 µL of Droplet Generation Oil for Probes (ddPCR™ 96-Well Plates #12001925, Bio-Rad). The entire emulsion volume was transferred from the cartridge to a 96-well PCR plate (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... TMEM87A in buffer 1 was exchanged into soybean l-α-phosphatidylcholine (Soy PC, MillaporeSigma) with the addition of Biobeads SM2 (Bio-Rad) and an hour incubation at a protein:lipid ratio of 1:10 (corresponding to 0.4 mg purified TMEM87A and 4 mg of Soy PC lipid or 1:50 in buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was soaked with Chemi-Lumi One L or Chemi-Lumi One Super (Nacalai Tesque) for the signal detection using ChemiDoc XRS+ (Bio-Rad Laboratories).