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Citations for Bio-Rad :
1151 - 1200 of 2194 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Proteins were reduced with 1 mM TCEP for 1 h then passed through a 10DG desalting column (Bio-Rad, Hercules, CA) to remove the TCEP ...
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bioRxiv - Plant Biology 2022Quote: ... Infiltrated leaves were infiltrated with 100 µM D-luciferin and LUC activity was quantified after 48 h using a low-light CCD imaging apparatus (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... conditioned culture medium was collected after 24 h and assessed by Luminex assay (Bio-techne) and a Bioplex-200 machine (Bio-Rad). Bioplex manager 6.1 software was used to determine analyte concentrations.
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bioRxiv - Biochemistry 2022Quote: ... Dialysis was repeated against another 1 L of low-salt buffer for 3 h before application to a MonoQ (MonoQ HR 10/10, Pharmacia) FPLC column (Biologic Duoflow system, Bio-Rad). The sample was eluted over 80 mL in 20 mM piperazine by applying a gradient from 0% to 30% of a high-salt buffer (20 mM piperazine ...
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bioRxiv - Molecular Biology 2023Quote: ... blots were incubated with secondary antibodies for 1 h and were consequently developed using Clarity Western ECL and Clarity Max Western ECL substrates (Bio-Rad). Protein bands were visualized on the ChemidocTM MP imaging system (Bio-Rad).
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were run at 4V for 16 h with a 1 – 10 running ratio in TBE buffer using a CHEF-DRII system (Bio-Rad). The gel was stained by 0.005% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... for 2 h (for the ERGIC-53 ΔΗ34 mutant) The beads were transferred into a gravity flow column (Econo-column, Bio-Rad) and washed with 20 CV of buffer A supplemented with 0.02% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Bio-Rad #1706515) at room temperature for 1 h on a shaker before being developed by Western Lightning Plus-ECL (Bio-Rad) at 1:1 ratio ...
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bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
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bioRxiv - Cell Biology 2023Quote: ... before being re-incubated with the secondary antibodies at room temperatures for 2 h and then subjected to the imaging of immunoblots by Bio-Rad instrument ...
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bioRxiv - Biochemistry 2023Quote: ... The slurry was gently stirred for 3 h to allow binding and then loaded into a glass Econo-Column (Bio-Rad). The column was washed with 10 Column Volumes (CV ...
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bioRxiv - Bioengineering 2022Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Cell Biology 2022Quote: ... The OD595 for each strain was recorded at the beginning and end of an 11-h incubation period at 30°C using an iMark Microplate Absorbance Reader (Bio-Rad). A detailed outline of the SGA procedure is included in Table S4.
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Cell Biology 2023Quote: ... plates were incubated at 25 °C for 30 h before imaging on a scanner and transilluminator (Bio-Rad Gel Doc XR+).
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad). The membranes were then washed three times (15 min each at room temperature) ...
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bioRxiv - Molecular Biology 2023Quote: ... embryos surviving for 24 h post- injection were incubated in 100 µl of 5 % Chelex® 100 Molecular Biology Grade Resin (BioRad) and 50 µl of Proteinase K Solution (20 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with primary antibodies at 4°C overnight and subsequently incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and all images were acquired using a ChemiDoc XRS+ system (Bio-Rad). Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated at RT for 1 h with HRP-linked secondary antibodies (Bio-Rad 170-6515 and 172-1011, 1:5000), imaged via chemiluminescence on a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
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bioRxiv - Biophysics 2024Quote: ... containing 20 mM MgCl2 and exposed to a thermal annealing ramp (65 °C, 15’; 58-53 °C, 1 h/°C; stored at 20 °C) in a Tetrad2 (Bio-Rad) thermocycler ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then separated by 20% Urea-PAGE gel at 250 V for 1 h and the fluorescent signal was captured by the ChemiDoc System (Bio-Rad). For protein detection ...
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bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
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bioRxiv - Cell Biology 2023Quote: ... 1mg cell lysate or 500 µg cytosolic or nuclear fraction were incubated with 2μg antibody or normal IgG and pulled down by 5µL protein G-magnetic beads (Bio-Rad). Samples were then pulled down by protein-G-magnetic beads and subjected to WB or proteomics analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were probed with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:10,000, 52200336 and 52200341, Kirkegaard & Perry Laboratories) and detected by enhanced chemiluminescence using a Chemidoc (BioRad) gel imaging system ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse-HRP and rabbit-HRP secondary antibodies were purchased from BioRad Laboratories (Hercules ...
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bioRxiv - Microbiology 2020Quote: ... probed with goat α-rabbit HRP-conjugated secondary antibody (Bio-Rad) at a 1:2000 dilution in TBS-T for 45 min at room temperature with shaking ...
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bioRxiv - Microbiology 2023Quote: ... pneumoniae strains previously incubated in 25% of baby rabbit complement (BioRad) at 37°C for 30 mins were added to fresh human neutrophils extracted from blood from healthy volunteers using MACSxpress kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... HRP-labeled α-rabbit secondary 1:10,000 (BioRAD Catalog #170-6515); and/or HRP-labeled α-mouse secondary (Cell Signaling Technology Catalog #7076S) ...
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bioRxiv - Neuroscience 2021Quote: ... PCR reactions were carried out in a final volume of 20 ◻ l reactions containing iQ Supermix (Bio-Rad), 5 nM FITC dye ...
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bioRxiv - Genetics 2019Quote: ... 10 mM L-serine) to a UapA concentration of 20 μM using Micro Bio-Spin 6 columns (Bio-Rad). UapA was loaded into gold coated capillaries and the protein sprayed into a Synapt G2-Si (Waters ...
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bioRxiv - Microbiology 2021Quote: ... SOB motility plates (containing 10-4 M IPTG and 2.4 g/L MgSO4) at 37°C and pictures were taken using a Gel Doc (Biorad) imager at the beginning and the end of the linear swimming motility period (from 5 to 8 hours) ...
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bioRxiv - Cell Biology 2023Quote: ... IgM-containing Capto L fractions were pooled and subjected to cation exchange chromatography using Nuvia HR-S (Bio-Rad) with an equilibrating and wash buffer (20 mM acetate ...
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bioRxiv - Neuroscience 2021Quote: ... were mixed with filtered monomeric α-syn WT (30 μM) and ThT (10 μM) and were incubated at 55°C for 5 h in a PCR machine (Bio-rad, C1000) for amplification.[3] Fractions comprised of ≥ 76% of its original volume were mixed with α-syn and ThT to reach a final volume of 20 μL per reaction ...
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bioRxiv - Plant Biology 2019Quote: ... A Bio-Rad Aminex HPX-87H (300 x 7.8 mm, 9 μm particle size) column with a Micro-Guard Cation H guard column (Bio-Rad, Watford, UK) was used to achieve separation with an isocratic gradient of 5 mM sulfuric acid ...
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bioRxiv - Physiology 2020Quote: ... Blood amino acids were extracted from 500 uL of supernatant by cation exchange chromatography (Dowex AG 50W-8X, 100–200 mesh H+ form; Bio-Rad Laboratories). Phenylalanine enrichments were determined by gas chromatography–mass spectrometry (GC-MS ...
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bioRxiv - Microbiology 2021Quote: ... the plates were incubated at 37°C for 24 h in ambient air and growth was assessed at OD595 using microplate reader (iMark, Bio-Rad, Japan). The lowest concentration that completely prevented bacterial growth was categorized as MIC.
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Genomics 2019Quote: The whole genome amplification was monitored in real time by detection of SYTO13 fluorescence every 15 minutes for 16 h using a Chromo4 real-time PCR instrument (Bio-Rad, USA) or a FLUOstar®Omega plate reader (BMG Labtech ...
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bioRxiv - Microbiology 2019Quote: ... pH 8 for 1 h at 37°C and then loaded into the wells of 1% pulse-field certified agarose gel (Bio-Rad Laboratories) in 0.5X TBE (45mM Tris HCl ...
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bioRxiv - Microbiology 2020Quote: ... The development of the colonies of the fluorescently labeled Trichoderma strains was monitored by making regular (every 24 h) images in a Bio-Rad ChemiDoc MP system (Bio-Rad, USA) equipped with multiplex fluorescent channels ...
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bioRxiv - Microbiology 2020Quote: ... and purified from remaining salts using Dowex 50 W X 8 50-100 mesh (Bio-Rad, 10 x 1 cm, H+ form) equilibrated with water ...
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bioRxiv - Immunology 2019Quote: ... Upfront tagmentation of ~35,000 nuclei was performed for 1 h at 37°C in a C1000 Touch thermal cycler with 96-Deep Well Reaction Module (Bio-Rad, 1851197). Tagmented nuclei were mixed the master mix and loaded onto a Chromium Chip with Gel beads and partitioning oil on different wells ...
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Synthetic Biology 2022Quote: ... The solid cultures were grown at 30ºC without shaking for 48 h and pictures of cell plates were taken using the colorimetric mode of Chemidoc Touch Imaging Systems (Bio-Rad, USA). All halo assays were conducted in three biological replicates.
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bioRxiv - Neuroscience 2022Quote: ... The gel was subject to 100 V for 3.5 h and the gel underwent semi- dry transfer by a Trans-Blot® Turbo™ Transfer System (Bio-Rad) onto a 0.2 µm polyvinylidene fluoride membrane at 1.3 A for 11 min ...
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bioRxiv - Cell Biology 2023Quote: ... wre incubated for 1 h at RT and membranes were revealed by using ECL reagents with the ChemiDoc imager (Bio-Rad Laboratories).