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Citations for Bio-Rad :
1201 - 1250 of 2194 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: Protein was separated by electrophoresis on 12% SDSCPAGE and transferred to a PVDF (polyvinylidene difluoride) membrane at 100 mA for 2 h using a Mini-PROTEAN Tetra system (Bio-Rad, USA). For detection of PpSerpin1 isoform proteins or PrPAP1 ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated for 1 h in water with 3X GelRed (Phenix Research Products) and imaged on ChemiDoc XRS imaging system (Bio-Rad Laboratories) and Quantity One imaging software (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2023Quote: ... wre incubated for 1 h at RT and membranes were revealed by using ECL reagents with the ChemiDoc imager (Bio-Rad Laboratories).
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bioRxiv - Pathology 2019Quote: ... Bands corresponding to EPCR and the housekeeping protein β-actin (mouse IgG #NB600-501, Novus, at 1:40,000) were detected by the chemiluminescence method (Clarity Western ECL Bio-Rad) and finally densitometrically measured by ImageJ 1.6.0 software.
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad; 1:2000) and goat ⍰ -rabbit IgG HRP conjugate (170-6515 from Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... was used at a dilution of 1:2,000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:2,000) followed by a chemiluminescence reaction (Clarity Western ECL Substrate; Bio-rad). For the SS-TOM lines analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Goat α-mouse secondary IgG-HRP antibody was used at a 1:5,000 dilution (BioRad Laboratories, Hercules, CA, 170-6516). Phospho-MAPK antibodies were incubated in 1X TBST (10 mM TRIS-HCl pH 8 ...
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bioRxiv - Microbiology 2019Quote: ... The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences) for 2h at 4°C using a disposable chromatography column (BioRad). The IgG-Sepharose column was washed with 35 ml of protein A binding buffer (10 mM Tris–HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and Mouse IgG HRP-linked whole Ab (Cytiva), then imaged using (ECL Select, Cytiva) with a ChemiDoc MP (Bio-Rad). For gel quantification ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Bioengineering 2024Quote: ... Each column was calibrated using 660 kDa to 1.35 kDa molecular weight protein standards (thyroglobulin, IgG, ovalbumin, myoglobin, and vitamin B12) (Bio-Rad). Following peak analysis by SDS-PAGE ...
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bioRxiv - Bioengineering 2020Quote: ... A 10 μL cell-free sample was injected into a BioRad chromatographic column (Aminex HPX-87H, 7.8 mm 300 mm, Biorad, USA). The HPLC was equipped with a P680 pump ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized using 600 ng input total RNA per 40 ¼l reaction with the iScript cDNA Synthesis Kit (BioRad), cycling for 5 min at 25°C ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were dialyzed in HEPES sodium salt-HCl (75 mM, pH 7.0) with Chelex-100 resin (5 g/L, Bio-Rad) to remove any divalent metal contaminants ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... The 20 μL reaction mix contained 5 μL sample or standard DNA together with 10 μL 2x SsoAdvanced Universal Probes Supermix (Biorad), 1 μL of each primer (5 μM stock solution) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of the supernatant was used to normalize the level of total protein in each sample via BCA assay (BioRad) and the supernatant was then flash frozen on dry ice ...
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bioRxiv - Biophysics 2021Quote: ... folding was quenched by taking 10 μL of sample and adding it to 10 μL of 2x Laemmli SDS gel-loading buffer without reducing agent (BioRad), vortexed and stored at 4°C until analysis ...
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bioRxiv - Bioengineering 2019Quote: ... Triplicate PCR reactions were performed in 20 µl final volume with 10 ng of fragmented gDNA using the ddPCR Supermix for Probes (No dUTP) master mix (Biorad) with 11-11 pmol primers and 50-50 pmol of TaqMan probes (for sequences of primers and probes see Table 1 below) ...
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bioRxiv - Bioengineering 2020Quote: 50 μL of PCR mastermix solution in Nuclease-free ultrapure water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
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bioRxiv - Immunology 2021Quote: ... 200 µL of bead mix solution were added to all wells and washed using a Bio-Plex Pro Wash Station (BIORAD). 25 µL of Universal Assay Buffer were added to all wells ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 μL of PCR1 reaction (2 ng) were combined for the qPCR reaction using CFX 96 Touch Biorad qPCR thermocycler (Biorad). Δct was calculated by subtracting the mean Cq of each sgRNA to a non-targeting control ...
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μl of the sample were heated at 95°C for 5 min and resuspended in 4x Laemmli sample buffer (BioRad)/50 mM DTT before SDS-PAGE.
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bioRxiv - Bioengineering 2024Quote: ... Fully expanded leaves from in vitro cultured Nicotiana tabacum plants (L. cv. Petite Havana) were bombarded with 50 μg of 0.6-μm gold particles (Bio-Rad) coated with 10 μg of plasmid DNA using 1,100 psi rupture discs (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated for 24 h in an incubator and the optical density was read at 595nm in a microtitre plate reader (BioRad i-Mark, Japan). Growth inhibition percentage was calculated by using the formula:
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bioRxiv - Microbiology 2021Quote: ... we filter-sterilized 250 µl of the supernatant fluid into 1-ml HPLC vials and measured their organic acid content in a Shimadzu 20A HPLC equipped with an Aminex HPX-87H column and a Micro-Guard cation H+ guard column (Bio-Rad, Hercules, CA) at 55°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% skimmed milk in TBST for 30 min at room temperature then incubated with primary antibodies overnight at 4°C and secondary antibody conjugated with horseradish peroxidase (HRP) for 1 h at room temperature before visualization by ECL Plus Western blotting detection reagent (Bio-Rad, Hercules, CA). Semi-quantification of the bands were performed using NIH Image J software.
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bioRxiv - Microbiology 2021Quote: Supernatant glucose and arabinose concentrations were analyzed by high-performance liquid chromatography (Waters Acquity UPLC® H-Class System) using an ion-exchange Aminex HPX-87H column (7.8 × 300 mm, Bio-Rad Laboratories N.V.). The analysis was carried out with an isocratic flow rate of 0.6 mL min−1 for 25 min at 50°C ...
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bioRxiv - Cell Biology 2020Quote: ... fixed in 4% paraformaldehyde for 2 h at 4 °C and embedded in PBS containing 3% low-melting point agarose (Bio-Rad, California, USA). The agarose gels containing the fixed organoids were processed in a standard automated tissue histology processor ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4°C and for 1 h at room temperature (RT) with secondary horseradish peroxidase conjugated antibodies (Bio-Rad, 1:3000). Membranes were then developed using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were resolved by SDS-PAGE and transferred to a PVDF membrane by tank blotting at 150 mA for 2 h using the Miniprotean III System from Bio-Rad (Hercules, USA). The membrane was blocked for 1 h with 5% skim milk in PBS containing 0.05% Tween-20 ...
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bioRxiv - Microbiology 2020Quote: ... the extent of bacterial growth after 18 h incubation was also determined by obtaining OD595nm measurements using a Bio-Rad iMark microplate absorbance reader (Bio-Rad Laboratories, USA).
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bioRxiv - Cell Biology 2022Quote: ... Expression of tGFP was monitored for 5 h at 30°C using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).
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bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... The neutralized sample was then loaded onto a packed ion exchange resin (DOWEX H+) in a poly-prep chromatography column (Bio-Rad, 731-1550). The column was then washed with 5% acetic acid ...
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bioRxiv - Neuroscience 2021Quote: ... followed by secondary peroxidase-conjugated antibodies produced in rabbit (1:10,000; Biorad). Immunoreactivity was detected by enhanced chemiluminescence (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated antibodies against mouse and rabbit were purchased from Bio-Rad Laboratories (1:5000)
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and RBD-specified single memory B cells were gated by IgG+RBD+ followed by single-cell sorting into 96-well PCR plates (free of DNase and RNase, Bio-Rad) containing 10μl lysis buffer (1X reverse transcriptase reaction buffer containing RNAse inhibitor) ...
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bioRxiv - Biochemistry 2022Quote: ... Input and MOV10/IgG IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed through Western blot using specific antibodies.
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bioRxiv - Biochemistry 2022Quote: ... MOV10/IgG IP-ed samples were eluted from the beads in 30 µL of XT buffer (Cat. No. 1610791, Bio-Rad) through heating at 95 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Neuroscience 2019Quote: ... The concentration of IgG present after dialysis was determined using a modified Lowry assay (DC protein assay, BioRad, Hemel Hempstead, UK) and adjusted by dilution with Hartmann’s or by dialysis against a sucrose solution (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2019Quote: ... was used at a dilution of 1:1.000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:10.000) followed by a chemiluminescence reaction (Clarity ™ Western ECL Substrate, BIO-RAD).
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (rat (IgM)/LM20 and LM19 mouse (IgG)/2F4 (in 5% milk PBS) and after washing chemiluminescence induced with Clarity Western ECL Substrate (Biorad, #1705061) and detected with ChemiDoc imaging system (Biorad).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and counted in the presence of Trypan Blue using the TC20™ Automated Cell counter (Bio-Rad) (24h floating cell count) ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... 1.5 μl for bound franction for GFP detection) were loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.